Markers and kit for implementing screening, diagnosis and therapeutic efficiency evaluation of chronic periodontitis

A technology for chronic periodontitis and efficacy evaluation, applied in the field of molecular biology, can solve the problem of not being able to timely and accurately indicate the occurrence, development and changes of periodontitis

Active Publication Date: 2018-12-04
东莞市第五人民医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the clinical evaluation indicators for the severity of periodontitis mainly include: periodontal probing depth (PD), sulcus bleeding index (SBI) and X-ray, etc. However, these indicators can only represent the final result of a single lesion, and are not Can not timely and accurately indicate the occurrence and development of periodontitis

Method used

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  • Markers and kit for implementing screening, diagnosis and therapeutic efficiency evaluation of chronic periodontitis
  • Markers and kit for implementing screening, diagnosis and therapeutic efficiency evaluation of chronic periodontitis
  • Markers and kit for implementing screening, diagnosis and therapeutic efficiency evaluation of chronic periodontitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. The relative expression levels of p19mRNA, p40mRNA, p35mRNA, and EBI3mRNA in patients with chronic periodontitis were significantly higher than those in healthy volunteers

[0035] Tissue source: 30 cases of periodontitis patient group (including 13 cases of severe chronic periodontitis, 17 cases of moderate chronic periodontitis, and medium The teeth with high-degree periodontitis were extracted for implantation or restoration. The normal gingival tissue of 30 patients with impacted tooth extraction was the healthy volunteer group. The periodontitis patient group and the healthy volunteer group were in gender, age, white blood cell There was no significant difference in the content and the content of IgG antibody against Porphyromonas gingivalis.

[0036] Real-time PCR detects the relative expression of p19mRNA, p40mRNA, p35mRNA and EBI3mRNA in the gingival tissues of patients with chronic periodontitis and gingival tissues of healthy volunteers. The specific ste...

Embodiment 2

[0046] Example 2. The relative expression levels of p40mRNA and EBI3mRNA in patients with severe chronic periodontitis were significantly higher than those in patients with moderate chronic periodontitis

[0047] According to the severity of periodontitis involving the teeth, chronic periodontitis can be divided into mild, moderate and severe. In order to explore the influence of markers on the course of chronic periodontitis, the test results of Example 1 are further analyzed. The relative expression levels of p19mRNA, p40mRNA, p35mRNA and EBI3mRNA in the gingival tissue of patients with severe chronic periodontitis are as follows: figure 2 Shown: the relative expression of p40mRNA and EBI3mRNA in the gingival tissue of patients with severe CP was significantly higher than that of patients with moderate CP (P 0.05); it can be seen that p40mRNA and EBI3mRNA can be used as markers for the diagnosis of chronic periodontitis. Especially used to distinguish severe and moderate chroni...

Embodiment 3

[0048] Example 3. Use of multiple markers in the diagnosis of chronic periodontitis

[0049] Based on the above findings, 50 gingival tissues of patients with chronic periodontitis and 50 gingival tissues of healthy volunteers were collected, and the relative expressions of p19mRNA, p40mRNA, p35mRNA and EBI3mRNA were detected using the Real-time PCR primers and method of Example 1 (GAPDH is the internal reference ), perform single index diagnostic analysis and multiple index combination diagnostic analysis on the test results to evaluate diagnostic accuracy; among them, the criteria for determining chronic periodontitis are: relative expression of p19mRNA≥0.9, relative expression of p40mRNA≥ 0.9, relative expression of p35mRNA≥0.8, relative expression of EBI3mRNA≥2.0, accuracy = correct number of index test samples / total number of tests. The results are shown in Table 2 below. It can be seen that the marker of the present invention can be improved to a certain extent as a combinat...

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Abstract

The invention discloses markers and a kit for implementing screening, diagnosis and therapeutic efficiency evaluation of chronic periodontitis. The invention discovers the molecular markers which arerelated to diagnosis and severe disease course classification of chronic periodontitis, wherein the molecular markers include p19mRNA, p40mRNA, p35mRNA and EBI3mRNA, as well as quantitative detectionof the molecular markers and a combination of the molecular markers. The markers, which are applicable to diagnosis of the chronic periodontitis, are expected to be used for preparing the kit for implementing screening, diagnosis and therapeutic efficiency evaluation of the chronic periodontitis.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and more specifically, relates to markers and kits for chronic periodontitis screening, diagnosis, and efficacy evaluation. Background technique [0002] Periodontitis is a chronic non-specific infectious disease caused by periodontal bacterial infection, which leads to the destruction of periodontal attachment tissue and alveolar bone. At present, periodontitis has a high incidence and prevalence rate all over the world. 5-20% of adults worldwide suffer from severe periodontitis. However, there are various types of periodontitis in children and minors, mainly invasive. Periodontitis, chronic periodontitis (Chronic periodontitis, CP) and periodontitis complicated by various systemic diseases are the main ones. Periodontitis is extremely harmful, leading to inflammation of the periodontal tissue, formation of periodontal pockets, progressive loss of attachment and alveolar bone resorption, and eventua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2800/18
Inventor 曾今诚蔡树玉梁艳芳林晓悦林碧华
Owner 东莞市第五人民医院
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