29 results about "Heterozygous genotype" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for early identification of the cold resistance of an actinidia chinensis tree body in the dormancy period, and the SNP molecular marker is located at the Chr69297479 position on the No.6 chromosome of the actinidia chinensis and is closely associated with the cold-resistant phenotype median lethal temperature (LT50) of the actinidia chinensis. The method can be used for identifying the tree body dormancy period cold resistance of natural population and hybrid filial generation of actinidia chinensis. The homozygous genotype (CC) of the SNP locus corresponds to a high-cold-resistance individual in the dormancy period of an actinidia chinensis tree body, and the heterozygous genotype (TC) of the SNP locus corresponds to a low-cold-resistance individual. The cold resistance prediction accuracy in the F1-generation hybrid population reaches 94.94%, and the prediction accuracy in the natural population reaches 96.15%. The molecular marker detection method disclosed by the invention is convenient and rapid, has good specificity and high accuracy, is not influenced by climate, environment and the like, can rapidly and effectively evaluate the cold resistance of natural resources and hybrid filial generations of the actinidia chinensis, and provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis.

Mouse models of obesity associated with human MC4R missense mutations are provided. Human mutations introduced with CRISPR / Cas9 into the mouse MC4R gene lead to an severely obese mouse phenotype in mice with a heterozygous genotype.

The application relates to a co-dominant molecular marker of a potato long-day adaptation gene StCDF1.4 and application thereof. The molecular marker can identify a StCDF1.4 homozygous or heterozygous genotype alone, and makes up the shortcoming that an existing molecular marker can distinguish StCDF1.4 genotype types only by combining multiple pairs of primers.

The invention relates to a CAPS (cleaved amplified polymorphic sequence) molecular marker for identifying the content of rosa chinensis aroma leaf acetate and application of the CAPS molecular marker, and relates to the technical field of molecular breeding, the marker is located at the 27954680 site of the third chromosome of a rosa chinensis genome, and the nucleotide sequence of the CAPS molecular marker for identifying the content of rosa chinensis aroma leaf acetate is shown as SEQ ID No.1. DNA fragments with the length of 447 bp are obtained through amplification of a specific primer pair, and genotypes are distinguished by combining DdeI enzyme digestion analysis: after enzyme digestion, the generated 447 bp and 274 bp fragments are low-content heterozygous types (T / A), and a 447 bp single band is retained as a high-content homozygous type (T / T). The genetic difference of the leaf acetate content of the Chinese rose can be rapidly identified, the flower fragrance character screening efficiency is remarkably improved, the breeding cost is reduced, an efficient molecular tool is provided for high-fragrance Chinese rose variety breeding, and the method has important application value.

The invention relates to the technical field of plant molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to a pitted surface of pepper and application. The SNP molecular marker can stably and accurately distinguish pitted surface (G / G), smooth surface (C / C) and heterozygous (G / C) genotypes of capsicum fruits, the marker typing and phenotype consistency is high, the accuracy rate reaches 90% or above, and the SNP molecular marker is remarkably superior to an existing phenotype-dependent observation method. Moreover, the SNP molecular marker can be used for identifying the pitted surface character of the fruit in the early stage of the plant, so that character judgment after fruit ripening is avoided, the breeding period can be effectively shortened, and the breeding efficiency can be improved. The SNP molecular marker can be directly applied to molecular breeding of pepper pitted surface characters, can provide reliable technical support for breeding of high-quality varieties, and has high practical value and popularization and application prospects.

The application discloses a SNP molecular marker related to a white spot syndrome virus (WSSV) tolerance trait of Procambarus clarkii, which is located in a 3rd intron region of a TRIM gene, specifically, a T>C type mutation at 585bp of a DNA sequence shown in SEQ ID NO. 1, wherein a T / C heterozygous genotype has significant correlation with the WSSV tolerance trait of the Procambarus clarkii. The application provides a favorable marker for molecular selection of the Procambarus clarkii, and can accelerate the selection process of the disease-resistant variety of the Procambarus clarkii.

The present application belongs to the technical field of sample pollution evaluation, and discloses a sample cross-contamination evaluation method, device, equipment and medium, comprising: performing quality control on batch sequencing data, and performing mutation site detection and genetic marker typing according to a human reference genome sequence and a site interval file; according to the genetic marker typing result and the site interval file, the number of heterozygous genotype sites is counted and the sample heterozygosity is calculated, according to the sample heterozygosity and a preset threshold, the sample contamination state is determined; according to the copy number estimation of the sample, the allele frequency is diploid standardized correction; based on the allele frequency in the genetic marker typing result and the mutation site detection result, the cross-contamination relationship between each two samples is analyzed by using identification rules to determine the pollution source; the site for calculating the pollution proportion is selected, and the weighted average algorithm is used to calculate the pollution proportion. The sample pollution degree can be accurately estimated and the pollution source sample can be identified.

This invention provides a rice gene Bph54 for resistance to brown planthopper and its application. The rice provided by this invention... Bph54 Genes are of great significance for breeding new rice varieties resistant to brown planthoppers. This invention successfully cloned the rice Bph54 gene using map-based cloning, and performed functional annotation and identification. This gene exhibits complete dominance, and both homozygous and heterozygous genotypes confer equal resistance to brown planthoppers in rice. This characteristic is beneficial for the application of this gene in breeding. Based on this, the Bph54 gene can be introduced into brown planthopper-susceptible plant varieties through traditional breeding techniques such as hybridization and backcrossing, or through genetic engineering, to cultivate new varieties of plants resistant to brown planthoppers with stable genetic traits. This invention provides important germplasm resources for effectively reducing insect damage and achieving increased and stable crop yields.

The invention provides development and application of an anti-pyrithiobac-sodium KASP molecular marker, and belongs to the technical field of genetic typing. The invention provides a KASP typing primer group for detecting the SNP site of the millet anti-pyrithiobac-sodium gene, and verifies that the resistance of millet to pyrithiobac-sodium herbicide is caused by single SNP mutation of an ALS target site. The KASP primers are designed on the basis of SNP loci, the primer pair can be used for accurately distinguishing resistance, susceptibility and heterozygous genotypes, and the method has the advantages of being stable and reliable in result, easy and convenient to operate, rapid and high in automation degree. By adopting the molecular marker to assist breeding, the breeding of the pyrithiobac-sodium-resistant millet matching variety can be accelerated, the breeding period can be shortened, the breeding efficiency can be improved, and the high-throughput identification of the pyrithiobac-sodium-resistant herbicide genotype and the molecular marker to assist the breeding of herbicide-resistant millet are significant.

The invention relates to the technical field of plant genetic engineering, and discloses a rice brown planthopper resistant gene Bph56, a molecular marker closely linked with the same and application of the rice brown planthopper resistant gene Bph56. The rice Bph56 gene provided by the invention is of great significance for cultivating a new variety of brown planthopper resistant rice. The rice Bph56 gene is successfully cloned through a map-based cloning method and is subjected to function annotation and identification, the gene is completely dominant, the homozygous and heterozygous genotypes endow rice with equal brown planthopper resistance, and the characteristic is beneficial to application of the gene in breeding. On the basis, the Bph56 gene can be introduced into a plant variety susceptible to brown planthopper through traditional breeding technologies such as hybridization, backcross and the like or genetic engineering means, and a novel brown planthopper-resistant plant variety with stable hereditary characters is obtained through cultivation. The invention provides important germplasm resources for effectively reducing insect pests and realizing yield increase and stable yield of crops.

This invention discloses a SNP marker, detection primers, and applications for genes related to nitrite resistance in Litopenaeus vannamei. The SNP molecular marker is located at the 193bp site of the sequence shown in SEQ ID NO.2, with mutation types of A / A homozygous, G / G homozygous, and A / G heterozygous. Litopenaeus vannamei with the A / A homozygous SNP molecular marker exhibits significantly higher nitrite resistance than those with the G / G homozygous and A / G heterozygous genotypes. This invention accelerates the breeding process of superior nitrite-resistant Litopenaeus vannamei varieties by identifying SNP markers associated with nitrite resistance and applying these markers to establish a marker-assisted breeding method for nitrite resistance in Litopenaeus vannamei.

This invention belongs to the field of apple molecular genetics and breeding technology, and specifically relates to a gene that increases GABA content in apples, its SNP molecular marker, and its application. This invention, for the first time, utilizes GWAS analysis to identify the GABA-related regulatory gene MdHVA22g in apple fruit. The gDNA sequence of this gene is shown in SEQ ID NO.1, and the CDS sequence is shown in SEQ ID NO.2. A corresponding SNP molecular marker was developed by associating the SNP with position 678 of the gene's gDNA, with polymorphisms of A or C. Apples with a homozygous AA genotype at this site have higher GABA content than apples with homozygous CC and heterozygous AC genotypes. Furthermore, this invention utilizes this SNP to design dCAPS primers, which can effectively and rapidly distinguish the GABA content of apples from different germplasms, facilitating the breeding of high-GABA apple varieties.

A method of genotyping one or more genetic rare variants in a plurality of nucleic acid samples is described. The method comprises running one or more assays of the plurality of nucleic acid samples using at least one microarray comprising a plurality of probesets and generating assay data. Heterozygous genotypes in the plurality of nucleic acid samples are called and evaluated to identify any rare heterozygous genotype calls that are from a probeset having at least one probe, wherein the number of heterozygous genotype calls for the probeset does not exceed a maximum threshold value. Each identified rare heterozygous genotype call is evaluated to determine whether the identified rare heterozygous genotype call comprises a true rare heterozygous genotype call using a support vector machine prediction model or supervised machine learning classification model comprising a plurality of predictor values for identifying a true rare heterozygous genotype call.

The application discloses a method for creating a DH line of eggplant by using anther culture and application thereof, and relates to the technical field of plant breeding. The application establishes a morphological index of a flower bud based on a calyx lobe separation state, replaces a time-consuming microscope examination procedure, and optimizes a combination of heat treatment and low-temperature pretreatment, so that the embryoid induction rate is significantly improved. Finally, through strict ploidy identification and self-pollination purification, a DH line with consistent traits and stable heredity can be obtained, an efficient pure line source is provided for eggplant hybrid breeding, and efficient conversion from a heterozygous genotype plant to a homozygous DH line is realized.

Co-dominant molecular markers for long-day adaptation gene StCDF1.4 in potato and the use thereof, which belongs to the technical field of molecular biology. The co-dominant molecular marker alone may identify homozygous or heterozygous genotypes of StCDF1.4, thus overcoming the limitation of existing molecular markers that require combinations of multiple pairs of primers to distinguish genotypes of StCDF1.4.

The invention discloses a molecular marker related to the high lysine character of corn as well as a primer group and application of the molecular marker, and belongs to the technical field of plant molecular breeding. On the basis of whole genome re-sequencing comparison, an InDel molecular marker closely linked with a corn high-lysine key gene is found, and the marker is located in a corn chromosome 7. The invention provides a specific primer group for detecting the marker, PCR (Polymerase Chain Reaction) amplification is carried out by utilizing the primer group, and identification can be carried out through agarose gel electrophoresis according to the length polymorphism of an amplified fragment: an individual amplified with a 835bp band is a high-lysine genotype, an individual amplified with a 610bp band is a common genotype, and an individual amplified with the 610bp band is a high-lysine genotype. And the individual with the 835bp band and the 610bp band simultaneously appearing is a heterozygous genotype. Therefore, the invention provides an efficient and reliable technical tool for molecular marker-assisted selective breeding of high-lysine corn.

The invention relates to the technical field of molecular biology and genetic breeding, and discloses a PARMS primer of an SNP site significantly associated with brassica napus germination period drought tolerance and application of the PARMS primer, the SNP marker provided by the invention is used for identifying rape germplasm resources, and it is found that when the rape germplasm resources are treated for seven days under 22.5% PEG-6000 simulated drought stress conditions, the drought tolerance of the brassica napus can be significantly improved. The average germination rate (59.12%-66.37%) of an AA genotype material is remarkably higher than the average germination rate (29.84%-37.60%) of GG genotype and AG heterozygous genotype materials. The molecular marker can be used for early identification of the drought-enduring character of the rape and molecular marker-assisted selective breeding, the selection efficiency is improved, and the drought-enduring breeding process of the rape is accelerated.

The invention relates to the technical field of genome information analysis, in particular to a local pig optimal heterosis prediction method based on genome information. According to the method, pure parents with clear genetic backgrounds are accurately identified and screened out by utilizing high-density SNP marker information covering a whole genome, a reference population is constructed, large-scale hybridization propagation is simulated, structured virtual population data is formed, and multi-character phenotype data of filial generations are standardized and weighted, so that the genetic backgrounds of the filial generations are optimized, and the genetic backgrounds of the filial generations are optimized. The method comprises the following steps of: dividing a plurality of characteristic groups such as a production performance type and a reproductive performance type, and analyzing the correlation between phenotypic deviation and genotype in each group, so as to separate and quantify dominant effect caused by heterozygous genotype and recessive effect caused by homozygous genotype, construct explicit and recessive weight parameters, and determine the genetic relationship between the dominant effect and the recessive effect; finally, the non-additive effect parameters are superposed to a traditional additive breeding value model, direct quantitative prediction of heterosis is achieved, and therefore the accuracy and efficiency of hybrid combination matching are greatly improved.

The present invention provides a molecular marker identification method related to porcine sperm motility and its application, relating to the field of biotechnology. This application involves applying a substance that detects the SNP molecular marker rs3476761356 to the preparation of a product for predicting porcine sperm motility. The SNP molecular marker rs3476761356 is associated with porcine sperm motility, with the TA genotype being the dominant genotype. Selecting pigs with the TA heterozygous genotype for breeding can effectively improve porcine sperm motility and accelerate the genetic improvement of boar semen quality, providing important insights into pig performance evaluation and genetic breeding.

Provided are a codominant molecular marker of a potato long-day adaptive gene StCDF1.4 and a use of the codominant molecular marker, relating to the technical field of molecular biology. The codominant molecular marker alone is sufficient to identify the homozygous or heterozygous genotype of StCDF1.4, overcoming the shortcoming that existing molecular markers need combination of a plurality of pairs of primers to identify the genotype of StCDF1.4.

The invention belongs to the technical field of biotechnology and livestock breeding, and relates to an SNP molecular marker influencing the breeding traits of goats and application, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, the 102bp site in the SEQ ID NO.1 is G or C, and the breeding traits refer to the multiparous lambing number. According to the invention, the effective SNP molecular marker influencing the lambing number of the goats is found on the LGR4 gene for the first time and is used for identifying the multiparous lambing number of the goats. The goats are screened through the molecular marker, GG homozygous genotype individuals are reserved, CC homozygous genotype individuals and CG heterozygous genotype individuals are eliminated, and the reproductive performance of the goats can be improved.

The application belongs to the field of biotechnology and livestock breeding technology, and relates to a SNP molecular marker affecting goat reproductive traits and application, wherein the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO. 1, the 102th site in the sequence of SEQ ID NO. 1 is G or C, and the reproductive trait refers to the number of offspring of a goat. LGR4 The application finds an effective SNP molecular marker affecting the number of offspring of a goat, and is used for identifying the number of offspring of a goat. By screening goats through the molecular marker, GG homozygous genotype individuals are retained, and CC homozygous genotype individuals and CG heterozygous genotype individuals are eliminated, so that the reproductive performance of the goat can be improved.

The invention relates to the technical field of germplasm resources and F1 group identification, in particular to a primer combination for identifying the hardness of apple fruits, a product and application of the primer combination. A specific primer (a first primer pair) is designed to carry out common PCR verification on a sample, and the result shows that only small bands can be amplified from 40 F1 offspring with extremely high hardness and extremely low hardness, and the small bands are not consistent with the expected result of parent genetics. Therefore, the second primer pair is innovatively designed, whether the variation site exists or not is judged by judging whether a target band appears in a PCR product of the second primer pair or not, and if no band exists in the second round of PCR amplification, it can be judged that the site is a homozygous deletion genotype in a filial generation genome; and if a target band appears in the second round of PCR amplification, judging that the site is a hybrid genotype in the filial generation genome. Therefore, double PCR verification results of the two pairs of primers are innovatively put forward to jointly confirm the accurate genotype of the test sample.

The invention provides an STR molecular marker for identifying rapid growth type litopenaeus vannamei and application of the STR molecular marker. A nucleotide sequence of the STR molecular marker is shown as SEQ ID No.1, and the STR molecular marker is located in a 287126 region of a litopenaeus vannamei genome NW020870315.1; the hybrid genotype litopenaeus vannamei with the AGAT unit repeated for 6 times and the AGAT repeated for 7 times of the STR molecular marker is the rapid growth type litopenaeus vannamei. The STR molecular marker is obviously associated with the growth traits of the litopenaeus vannamei, and the repetition times of the STR molecular marker are obviously and positively associated with the growth phenotype of the litopenaeus vannamei, that is, the growth of the litopenaeus vannamei with a high copy number is faster, and the STR molecular marker [AGAT] n can be used for selecting individuals growing rapidly from the molecular level, so that the breeding efficiency of the litopenaeus vannamei is improved, and the breeding cost of the litopenaeus vannamei is reduced. The method is not limited by the growth stage, and can be used for early selection, so that the selection accuracy and efficiency are improved, and the breeding process of the growth speed is accelerated.

The present application relates to the technical field of plant genetic engineering, and discloses a rice brown planthopper resistance gene Bph56 and a closely linked molecular marker and application thereof. The rice gene provided by the present application has important significance for cultivating new rice varieties resistant to brown planthopper. The present application successfully clones the rice gene by a map-based cloning method, and performs functional annotation and identification on the gene. The gene exhibits complete dominance, and both homozygous and heterozygous genotypes of the gene confer the rice with equal resistance to brown planthopper, which is beneficial to the application of the gene in breeding. Based on this, the gene can be introduced into plant varieties susceptible to brown planthopper by traditional breeding techniques such as hybridization and backcrossing or genetic engineering means, and a new variety of plants resistant to brown planthopper with stable genetic traits can be cultivated. The present application provides important germplasm resources for effectively reducing insect pests and realizing crop yield increase and yield stability. Bph56 Bph56 Bph56 ​​​

The invention belongs to the technical field of molecular biology and molecular genetic identification, and particularly relates to a functional molecular marker of a sorghum grain weight gene HGW4 and application. The invention provides a molecular marker HGW4 for identifying the sorghum grain weight gene HGW4, and also provides a method for identifying the sorghum grain weight gene HGW4 by using the molecular marker HGW4, and the gene marker can be used for distinguishing a small grain homozygous genotype, a large grain homozygous genotype and a heterozygous genotype of sorghum.

This invention relates to the field of plant molecular breeding technology, specifically to a Kasp marker CC-1-KASP-147 closely linked to maize cob color and its application. The SNP locus corresponding to the Kasp marker is located at position 47834083 on maize chromosome 1, with a polymorphism of C / A. The primer set includes forward primer 1, forward primer 2, and reverse primer. The identification method involves extracting maize genomic DNA, performing PCR amplification using this primer set, and performing KASP genotyping on the amplified products. Genotypes are determined by clustering relative fluorescence values: C / C homozygous genotype corresponds to a white cob, A / A homozygous genotype corresponds to a pink or red cob, and C / A heterozygous genotype corresponds to a white, pink, or red cob. This invention has been validated in multiple environments, and the genotyping results show 100% agreement with the cob color phenotype. It eliminates the need for gel electrophoresis, can be detected at the seedling stage, significantly shortening the breeding cycle. It provides an efficient molecular auxiliary tool for the directional improvement of maize cob color and the breeding of mechanized grain-harvesting varieties, and has significant practical value.

This invention provides a method for detecting genes that resist bacterial blight in rice. Xa21 The InDel marker for genotypes and its application belongs to the field of rice breeding technology. The upstream primer sequence for the InDel marker is Xa21-InDel-F: 5'-TCTGCTCTACACTGCAATATGAAATGA-3', and the downstream primer sequence is Xa21-InDel-R: 5'-TAGTACCAAGTATTTTTCATGGTCAATG-3'. The Xa21-InDel marker was used to detect IRBB21 and 37 hybrid japonica rice restorer lines. The results showed that the InDel marker designed in this invention can accurately identify whether different varieties contain genes for resistance to bacterial blight. Xa21 And heterozygous genotype. This detection method has high accuracy and specificity. Xa21 Molecular marker-assisted selection of genes has strong practical applications in breeding.

The application provides application of a reagent for detecting SNP site rs11196224 of a TCF7L2 gene in preparation of a product for predicting a brain stroke morbidity risk of an individual. Specifically, there are three genotypes of the TCF7L2 rs11196224 site, which are CC type, CT type and TT type; and the prediction product is used for predicting that the morbidity risk of the individual suffering from brain stroke is reduced when the genotype of the TCF7L2 rs11196224 site of the individual is detected to be mutated from the wild genotype CC to the hybrid genotype CT and the homozygous genotype TT. The application firstly finds that the TCF7L2 rs11196224 has a significant correlation with the morbidity risk of brain stroke, and can realize early evaluation of a brain stroke population, thereby realizing early, rapid, accurate and large-scale screening of the brain stroke population.