59 results about "Heterozygous genotype" patented technology

The invention discloses a broiler growth character-associated molecular marker and an application thereof. A nucleotide sequence of the molecular marker is as shown in SEQ ID NO:1, and a T / C mononucleotide mutation is arranged at the 1834th bp of a second intron in the SEQ ID NO:1. The molecular marker can be applied to breeding of a medium-speed broiler and the application methodcomprises the steps of extracting and amplifying DNA of a to-be-detected broiler; carrying out sequencing and sequence alignment and typing on an amplified product; and selectively reserving an individual which is judged as a heterozygous genotype during breeding. The body weight of the heterozygous genotype individual screened through the molecular marker disclosed by the invention at D70, D77 and D84 is significantly higher than that of a homozygote individual, and the shin length at 77D and the shank circumference at 70D are also significantly higher than those of the homozygote individual. The molecular marker is beneficial to breeding of a dominant heterozygote broiler, and the production performance of the broiler is improved.

The invention discloses a genome breeding value estimation method for integrating dominant effect, which relates to the technical field of livestock and poultry genetic selection. The method comprisessteps identifying a reference group and a candidate group, the phenotype of the target traits of the reference population was determined, Genome-wide marker typing of reference population, quality control of gene marker of reference population, statistics of heterozygous marker deviation of reference population, formulation of genome marker re-coding rules, genome-wide marker typing of candidatepopulation, quality control of gene marker of candidate population, re-coding of genome marker and estimation of genome breeding value, etc. Based on the deviation degree between the phenotype of theheterozygous genotype and the phenotype of the homozygous genotype, the invention formulates coding rules, starts from the genomic marker end, re-codes the heterozygous genotype, causes the gene marker coding to include dominant effect, and then estimates the genomic breeding value. The invention is adapted to the needs of livestock and poultry genetic breeding, and can greatly improve the accuracy of genome estimation breeding value without increasing the complexity of the model.

The invention discloses a structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs. The SV177 is characterized in that the SV177 is positioned at chrX: 100561156-100562159 of a pig reference genome Sscrofa 10.2, missing genes of the SV177 are determined as D, and normal genes which are not missed are determined as A. When a detection technology provided by the invention is used for detecting the SV177 genotype of a sample, the large white pigs have three genotypes and give priority to the genes A which are not missed, and the Chinese indigenous pigs are free from a heterozygous genotype (DA) and give priority to the missing genes D; therefore, the SV177 can be used for distinguishing the varieties of the large white pigs and the Chinese indigenous pigs as a molecular marker.

The invention provides application of an SNP molecular marker in detecting the skin color character of a black-bone chicken. The nucleotide sequence of the molecular marker is shown as SEQ ID No.1, and the base mutation type at the 171st bp is A / G. The invention further provides a primer sequence for detecting an SNP locus and a PCR-RFLP method. The genotype of the locus can be distinguished based on the difference of an electrophoretic band after DraI enzyme digestion, and then the homozygosis and heterozygosis of a sample Fm gene can be judged. If the 342 bp band appears, the sample Fm gene is a GG homozygosis-based gene; if the two bands of 171 bp and 342 bp appear, the sample Fm gene is an AG heterozygosis-based gene; if the 171 bp band appears, the sample Fm gene is an AA recessive-homozygosis-based gene. By means of the SNP molecular marker and the detection method, the individual genotype of the black-bone chicken can be judged in the chickling stage, early selection and early elimination of the skin color character can be achieved, and thus significant practical value can be achieved on improving selection efficiency and saving breeding cost.

The invention relates to an animal cultivation method, in particular to a cultivation method for a green-shelled-egg chicken pure line. The method adopts Jiuyuan black chickens as materials, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity. The method comprises the following steps: 1) selection of parents; 2) production of homozygosis green shell gene individuals; 3) expanding propagation of colony. The method has the advantages that the construction of green-shelled-egg chicken homozygosis colony is realized, homozygosis genotype green-shelled-egg chickens can be bred by screening heterozygosis genotype at the natural conditions and adopting a scientific breeding scheme, quick expanding propagation can be realized, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity.

The invention relates to a method for simultaneously positioning multiple genes. The method comprises the steps of creating a BC1F1 group and an F2 group, screening a codominant polymorphic molecule mark, performing genotyping on the F2 group by means of the molecule mark, then classifying the materials with the same genotype to one group, investigating the phenotype difference between different groups and performing statistical analysis, performing verification by means of a hybrid genotype F2 single plant progeny, namely positioning a certain phenotype trait gene next to the specific molecule mark. The method of the invention has advantages of the positioning the quantitative character and qualitative character, improving group utilization efficiency and realizing large-scale gene positioning by emans of a phenomics-related facility.

The invention belongs to the technical field of rape breeding and molecular biology, and discloses a molecular marker closely related to the rape flower color traits and application of the molecular marker. The molecular marker comprises a forward primer and a reverse primer. With total DNA as a template, PCR amplification is performed on the molecular marker and electrophoretic separation is performed; a single plant only having a single stripe of 180 bp is a homozygous yellow-flower individual, a single plant only having a single stripe of 177 bp is a homozygous white-flower individual, anda single plant having the two stripes at the same time is a heterozygous individual. The molecular marker developed by the invention can be applied to identification and prediction of a white-flower variety and a yellow-flower variety of rape; the operation is simple and convenient; a method is easy to implement; the problem of identification between a heterozygous genotype and a homozygous genotype can be effectively solved; offspring selection is more pertinent; the selection efficiency is improved.

The invention provides a method for improving the Sunite sheep prolificacy by using GDF9 genes. The linkage of three sites -332, -534 and -779 is unbalanced. The method uses a PCR method for amplifying the Sunite sheep GDF9 gene GenBank Accession Number into 41771049 to 41771267 site nucleotide fragments (-534 site) of NC_019462; an amplification product is subjected to RFLP detection; if a striponly occurs in the 214bp position, the result proves that the genotype is CC; if the strips occur in 214bp, 129bp and 85bp positions, the result shows that the genotype is the heterozygous genotype CT; if the strips only occur in the 129 and 85bp positions, the result proves that the genotype is TT; the prolificacy of the Sunite sheep with the genotype of CT(-332 site), AG(-534 site) and CT(-779 site) is higher than that of the Sunite sheep with the genotype of CT(-332 site), AA(-534 site ) and CC(-779 site). The PCR-RFLP is used for fast, simply and conveniently detecting the haplotype genetic marker polymorphism; an accurate simple and convenient method is provided for improving the Sunite sheep prolificacy by using a molecular marker auxiliary breeding technology.

The invention belongs to the technical field of fish genetic marker screening and preparation, and more specifically relates to a genetic marker-related with immunity traits of hemorragic disease of grass carp. The genetic marker is obtained by screening C7N1 gene. The genetic marker is obtained by steps of grass carp individual level resistant colony and sensitive colony transcriptome sequencing, cell level resistance and sensitive cell transcriptome sequencing, correlation analysis, and infection validation, the genetic marker is shown as SEQ ID NO:1, a base substitution mutation of C7N1 gene at 2145th bit base, and the change of 715th amino acid of grass carp C7N1 gene is generated. The grass carp reovirus infection test shows that heterozygous gene-type grass carp mortality is obviously lower than the homozygotic-type grass carp mortality, and shows that the grass carp C7N1 gene base mutation is related with the grass carp hemorragic disease property. The novel genetic marker resource can be provided for seed selection for immunity traits of hemorragic disease of grass carp.

The invention proposes a method for increasing induction frequency of new inter-subspecific tetraploid rice germplasm. The method comprises the steps of by means of induction technique of rice genomepolyploidization, firstly obtaining an inter-subspecific hybrid population (F1) with a heterozygous genetic basis through sexual hybridization among cultivated rice subspecies; obtaining a heterozygous population (F2) with various genotypes through self-crossing; and inducing and creating the new inter-subspecific tetraploid rice germplasm, thereby improving the induction efficiency of the new inter-subspecific tetraploid rice germplasm. In a same experiment, if the concentration (0.1%) of the inducing agent and the induction time (72h) are specific values and the adopted experimental materialis of a heterozygous genotype, i.e., a genetic heterozygote, the probability of successfully inducing the genome polyploidization is relatively big, that is, in a same induction experiment, when thegenome polyploidization induction is simultaneously carried out on various genotype materials, the probability of successful induction is greatly increased.

The invention discloses a new soft rice waxy gene wx-C39 functional marker and application thereof, and belongs to the field of agricultural biological engineering. A molecular marker primer is WxC39-F:CGTCCCAGCTCGCCACCT and WxC39-R:CCACGCTGCACCGACCG, and if an amplification product is 173bp, it is indicated that wx-C39 homozygous genotype is carried, wherein the amylose content is about 5%; if 184bp is obtained through amplification, it is indicated that a homozygous genotype of Waxy is carried, wherein the amylose content is about 15%; if two bands are amplified simultaneously, it is indicated that a heterozygous genotype exists, wherein the amylose content is about 15%. The marker and a method can be used for filial generation genotype detection, and the soft rice breeding process can be accelerated conveniently. The method is high in accuracy, easy to operate, high in detection efficiency and low in cost, and has very high practical value.

The invention provides a method for rapid breeding of maize leafless tongue inbred lines with assistance of molecular markers. By virtue of two SSR molecular markers closely linked to a maize leaflesstongue gene lg1, a maize leafless tongue inbred line is obtained by hybridization, three backcrossing and molecular marker assisted foreground and background selection, and selfing. In the method, the heterozygous genotype Lg1lg1 is identified and screened in the process of backcrossing, thereby eliminating the step of selfing of backcross descendants, and compared with conventional breeding methods, the accuracy of selection is enhanced, and nearly half of the line selection time can be saved. At the same time, in view of the problem that the background recovery cycle of a traditional backcrossing method is long, the method, in combination with a method in which molecular markers assist in background selection, can be used to obtain a leafless tongue improved line with genetic backgroundbasically recovering to recurrent parent after 3 generations of backcrossing and 1 generation of selfing only.

The invention provides a method for detecting high temperature resistant genes TT1 of rice and a dCAPS marker thereof. The primer sequence is: TT1-dcaps-F:5'-AGTCGAAAGCAAGCACAACAGGATTATC-3', and TT1-dcaps-R:5'-AGCAGAAACACCGATTCGGAATCAC-3'. The PCR product is digested by restriction endonuclease BsaBI, and detected by 8% polyacrylamide gel electrophoresis and silver staining. A 143 bp band is detected as a rice material containing a high temperature resistant TT1CG14 gene. The marker TT1-dCAPS is verified by using a high temperature sensitive three-line hybrid indica rice line retention line 9B, a near-isogenic line NIL (CG14) containing a high temperature resistant allele TT1CG14, and hybrid F1 and F2 generation populations between the two, and the results show that the marker can accurately identify different TT1WYJ, TT1CG14 and heterozygous genotypes of rice TT1 genes, and can be used for molecular marker-assisted breeding of the TT1 genes.

The invention provides SNP molecular markers, primer pairs and a kit of FABP4 gene related to beef quality in Yanbian yellow cattle, and application of the SNP molecular markers, the primer pairs andthe kit, and belongs to the technical field of beef quality screening in yellow cattle. The SNP molecular markers of the FABP4 gene contain a nucleotide sequence of a polymorphism at the 3496 bp as anA / C site, a polymorphism at the 3533 bp as an A / T site, a polymorphism at the 3711 bp as a G / C site, a polymorphism at the 3745 bp as a T / C site and a polymorphism at the 3767 bp as a T / C site of theFABP4 gene. The above five polymorphic sites are located in the third exon, and are in a strong linkage disequilibrium state with similar genetic effects. When the SNP molecular markers are applied to screen breeding fat cattle, fat content and marble pattern are selected as the detection indicators for screening breeding fat cattle while the genotype is heterozygous.

The invention relates to a molecular marker closely linked with the myzus persicae resistance character of a cultivar source, a primer, application and a variety breeding method. In the research process of a novel myzus resistance type, namely a QMR myzus resistance material, of a kind of cultivar source, the molecular marker closely linked with the myzus persicae resistance character is found, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and the 72<nd>-91 sites from the 5' end are inserted or deleted. The molecular marker is closely linked with the myzus persicae resistance character, and when the 72<nd>-91 site from the 5' end of the sequence shown in SEQ ID NO.1 is an insertion homozygous genotype, a to-be-detected sample has a myzus sensing character; and when the 72<nd>-91 site from the 5' end of the sequence shown in SEQ ID NO.1 is a deletion homozygous genotype or an insertion / deletion heterozygous genotype, the to-be-detected sample has the myzus resistance character. By detecting the genotype of the molecular marker, whether a plant to be detected has myzus resistance or not can be effectively predicted in the early stage. The invention furtherprovides the primer for detecting the molecular marker and a myzus persicae resistance variety breeding method.

The present invention provides methods for diagnosing an immune-mediated disease, e.g., an autoimmune disease, an allergy or an inflammatory disease. Diagnosis is made by detecting a heterozygous or homozygous genotype of haptoglobin 2 or by detecting and quantifying pre-haptoglobin 2 mRNA or protein. After diagnosis, the disease may be treated by decreasing cell permeability leading to increased transepithelial electrical resistance, for example, by administering an antibody directed against single chain zonulin thereby inhibiting epidermal growth factor receptor and inhibiting proteinase-activated receptor 2 (PAR2). Also provided is a single step method for determining severity of an immune-mediated disease in a subject by identifying a genotype for haptoglobin 2 in a biological sample from the subject. A homozygous genotype correlates to 2 copies of zonulin and a more severe disease than a heterozygous genotype correlating to 1 copy of zonulin.

The invention discloses a rapid plant breeding method. The invention provides a method for obtaining a plant DH line, which comprises the following steps: hybridizing by taking a haploid induction line as a male parent and taking a heterozygous genotype dominant male sterile material or a photo-thermo-sensitive male sterile material or a sterile material subjected to chemical emasculation treatment as a female parent; and identifying and separating haploid from the F1 generation, doubling, and selecting a fertile doubled plant to obtain the target DH line. According to the rapid DH creation method provided by the invention, the period of age of pure line breeding of crops can be remarkably shortened, the workload is reduced, and the workload and cost of breeding are greatly reduced, so that the breeding efficiency of varieties is improved, and a technical support is provided for variety upgrading of new varieties.

The invention relates to a general codominance molecular marker for resisting nilaparvata lugens Stal BPH9 multiple alleles of rice, and a detection method and application of the general codominance molecular marker. The general molecular marker for resisting nilaparvata lugens Stal multiple alleles BPH1 / 10 / 18 / 21, BPH2 / 26, BPH7 and BPH9 of the rice, provided by the invention, is located at (22874098bp)th-(22874099bp)th position of a 12th chromosome of the rice, and the polymorphism is 5'-TG-3' / 5'-CC-3' / 5'-CA-3'. The general codominance molecular marker for resisting nilaparvata lugens Stal multiple alleles of rice provided by the invention can be generally used for identification of homozygous genotypes or heterozygous genotypes of the multiple alleles BPH1 / 10 / 18 / 21, BPH2 / 26, BPH7 and BPH9, and efficient accurate identification of BPH1 / 10 / 18 / 21, BPH2 / 26, BPH7 and BPH9 genotypes of nilaparvata lugens Stal resisting rice recourse is realized.

The invention relates to the technical field of agriculture, in particular to a system for culturing a regenerated haploid plant from winter jujube anther and a culture method thereof. The system comprises an anther culture medium, a primary callus culture medium and a secondary callus culture medium, wherein the anther culture medium consists of 1 / 2 of MS, 0.8-1.2mol / L of 2, 4-D, 18-22g / L of maltose and 3.2-3.7g / L of agar; the primary callus culture medium consists of MS, 0.8-1.2mg / L of TDZ, 18-22g / L of maltose and 3.2-3.7g / L of agar; the secondary callus culture medium consists of MS, 1.8-2.2mg / L of triacontanol, 18-22g / L of maltose and 3.2-3.7g / L of agar. The system is simple in composition and facilitates culture; through haploid breeding, jujube trees can also adopt seed propagation to substitute asexual propagation, and a pure material can be provided for genetic research on a jujube variety with a highly heterozygous genotype, so that the breeding period is shortened and the research on the genetic theory of the jujube trees is promoted.

The invention discloses a KASP marker primer group for detecting waxy genes of wheat, and belongs to the technical field of molecular breeding. Wx-A1 gene, Wx-B1 gene and Wx-D1 gene in wheat can be detected by arranging three KASP marker primer groups, fluorescence detection of the Wx-A1 gene and the Wx-D1 gene molecular markers of the wheat waxy genes is achieved by utilizing a KASP technical system, compared with gel electrophoresis detection, the detection efficiency is improved, batch and automation can be achieved, the advantages of being simple, convenient, capable of saving time and high in throughput are achieved, the codominant detection of the Wx-B1 gene is also realized, the generation of false negative detection results is avoided, and meanwhile, the distinguishing of dominant pure and heterozygous genotypes is realized.

The invention provides a molecular marker of wheat recessive nuclear male sterility mutant gene ms1 and an application of the molecular marker, and discloses a detection method of the molecular markerof the wheat recessive nuclear male sterility mutant gene ms1 and the application. Molecular markers of the four mutants ms1d. 1, ms1h, ms1m and ms1p are designed, and are all functional markers of the MS1 gene. The markers can specifically detect the wheat mutant ms1, the wild Ms1 gene and the mutant ms1 gene, and homozygous and heterozygous genotypes can be differentiated. The allelotype of thenuclear male sterility mutant gene ms1 or the fertile gene ms1 can be detected and marked accurately by the designed functional markers, and the molecular marker can be used for identification of wheat nuclear male sterile plant lines and screening of target single plants, and has important application value in transformation of the wheat male sterile line and sterile line hybrid seed productionand molecular marker-assisted breeding.

The invention discloses a functional marker AS-ALS for anti-imazethapyr herbicide genotype detection and an application of the functional marker AS-ALS. The marker comprises a primer combination F1N (AS-ALS-F1 and AS-ALSn-R2-2) and a primer combination F1M (AS-ALS-F1 and AS-ALSm-R2-1), the F1N can amplify a stripe sample, namely, an ALS-G genotype, the F1M can amplify a stripe sample, namely, an ALS-A genotype, both the F1N and the F1M can amplify a stripe sample, and namely, a heterozygous genotype of the ALS-G and the ALS-A. The marker and a method can be used for genotype detection of variety resources and filial generation and are beneficial to accelerating an herbicide-resistant breeding progress. The method is high in accuracy and detection efficiency, simple to operate and low in cost and has a high practical value.

The invention discloses a dCAPS (derived cleaved amplified polymorphic sequences) molecular marking method for rapidly screening imidazolinone herbicide resistant rice. Sequencing comparison proves that mutation of amino acid at a 627 locus of each ALS (Acetolactate synthase) gene into N (Asparagine) is a primary cause of resistance of 'Jinjing 818' to imidazolinone herbicides, the ALS gene carrying a resistance locus is named as ALS627N, and the ALS gene without carrying the locus is named as ALS627S. According to DNA (deoxyribonucleic acid) sequence differences, a novel functional markerdC-ALS-627 is developed. The marker can effectively distinguish ALS627N homozygotic type, ALS627S homozygotic type and hemizygous lines. In addition, herbicide spraying tests prove that the ALS627N is a dominant herbicide resistant gene. Rice varieties in different regions are detected by the marker to prove that the marker can be widely used for marker-assisted breeding of the ALS627N gene, canaccelerate culture of new imidazolinone herbicide resistant varieties and has important practical application values.

The invention discloses a specific CAPS marker for identifying a wild type, a heterozygous mutant and a homozygous mutant of a cucumber male sterility gene Csa3M006660. The specific CAPS marker is characterized in that the specific CAPS marker comprises a marker JD-CsaMS-CAPS2.0 for detecting a wild type locus, and a the nucleotide sequence of the marker JD-CsaMS-CAPS2.0 is shown as SEQ ID NO:1; and wild type genome DNA is shown as SEQ ID NO:2, and a mutant genome is shown as SEQ ID NO:3. By designing a method of combination of primer amplification, enzyme digestion and agarose gel electrophoresis, two characters of male sterility and male fertility in a cucumber 'YL-5' strain can be efficiently screened at the seedling stage. A method has the characteristics of short period, low cost andhigh efficiency, identifying can be conducted during on crossbreeding and, backcross segregation of colonies and the like, and a heterozygous genotype and a homozygous genotype are distinguished accurately, and the breeding efficiency is improved.

The invention relates to the technical field of plant genetic breeding, in particular to an SNP molecular marker associated with the hemi-cellulose content of raw ramie and application thereof. The invention provides an SNP molecular marker associated with the hemi-cellulose content of raw ramie. The molecular marker is positioned on chromosomes 1 of ramie. Through identification, significant correlation exists between the marker and ramie hemi-cellulose. The content of TT genotype hemi-cellulose is remarkably higher than that of CC genotype hemi-cellulose, and the content of heterozygous genotype hemi-cellulose is between those of the two genotypes. By identifying the genotype of the SNP site of the ramie variety, the hemi-cellulose level of raw ramie can be predicted. Therefore, the marker can be used for identifying ramie germplasm conforming to the breeding target in the early growth stage of ramie, and has important significance for early prediction and screening of the hemi-cellulose content level of raw ramie. The breeding efficiency is improved, and the breeding process is accelerated.

The invention discloses a molecular marker of a rice gelatinization temperature gene ALK as well as a primer and application of the molecular marker. Nucleotide sequences of the molecular marker are shown as SEQ ID NO.3 and SEQ ID NO.4. The nucleotide sequence of the high-alkali elimination rice amplification product is as shown in SEQ ID No.3, the nucleotide sequence of the medium-alkali elimination rice amplification product is as shown in SEQ ID No.4, and the heterozygous genotype rice material is simultaneously amplified into bands as shown in SEQ ID No.3 and SEQ ID No.4. According to the invention, the identification of the rice molecular marker assisted breeding high / medium alkali elimination value material can be rapidly detected by using a simple and reliable method.

The invention relates to an animal cultivation method, in particular to a cultivation method for a green-shelled-egg chicken pure line. The method adopts Jiuyuan black chickens as materials, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity. The method comprises the following steps: 1) selection of parents; 2) production of homozygosis green shell gene individuals; 3) expanding propagation of colony. The method has the advantages that the construction of green-shelled-egg chicken homozygosis colony is realized, homozygosis genotype green-shelled-egg chickens can be bred by screening heterozygosis genotype at the natural conditions and adopting a scientific breeding scheme, quick expanding propagation can be realized, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity.