1536 results about "Cladodes" patented technology

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

The invention discloses a pasania fungus culture substrate consisting of solid materials and water. The solid materials comprise the following components by weight percentage: 35 to 40 percent of summer cut mulberry twig chips, 35 to 40 percent of cotton straw chips, 18 to 20 percent of bran coat, 2.5 to 3 percent of corn flour, 1 to 1.2 percent of sugar, and 1.5 to 1.8 percent of gesso. The dosage ratio of the solid materials to the water is 1: (1.2-1.3). The substrate is packed by bags with the bag diameter of between 18 and 22 centimeters. The substrate is used for culturing pasania fungus, and can make full use of local discarded crop resources in accordance with local conditions without consuming wood. The substrate can reduce the cost and effectively improve the quality and yield of the pasania fungus. The economical benefit is improved by 15 to 20 percent than that of the prior pasania fungus culture substrate; therefore, the substrate has important popularization value.

The invention discloses an artificial cultivation method of hemsley rockvine root. The artificial cultivation method comprises the following steps of: 1) selecting an annual or biennial branch which vigorously grows and is well developed and not damaged by diseases or pests, cutting the branch with the length of 4 nodes, keeping 2 leaves on the upper part of the cutting, shearing a lower cut of the cutting into a smooth diagonal plane, and soaking in 500mg/L rooting powder solution; 2) selecting field sandy soil, obliquely cutting the cutting at an angle of 35 to 45 DEG with the ground with the depth of 2 nodes of a lower part, slightly compacting the surrounding substrate and immediately watering after the cutting is completed, keeping the cutting soil humid and keeping shading and ventilation within 15 days after the cutting; and 3) transplanting the cut seedlings to fields in March and April each year, controlling cultivation density, shading by using a sunshade screen in the cultivation period, and applying a calcium fertilizer after 15 days of transplantation. By the technical scheme, the hemsley rockvine root is successfully bred and cultivated, so that a wild hemsley rockvine root resource is protected, artificial excessive collection and digging are avoided, and the requirement of clinical and scientific research medication can be met.

The invention provides a systematic and standard method for cultivating Dragon fruit in greenhouses in North China. The method comprises the steps of selection of sunlight greenhouses with good thermal insulation performance, fielding planting, field management and the like. The method provided by the invention is appropriate to standardized dragon fruit cultivation in greenhouses in North China, and has the benefit that the existing greenhouse conditions of North China are utilized to cultivate tropical and subtropical fruit trees so as to enrich the variety of crops cultivatable to facilities in North China. Dragon fruit grown according to the method provided by the invention has plump branches and smooth and juicy flesh, and has the advantages of elegant shape, excellent color, sweet flavor, good taste and high output.

The invention discloses a method for rapidly raising high-quality 'three-current' (raising stocks, grafting and moving seedlings out of nurseries in the current year) well-bred seedlings of Carya illinoensis. The method disclosed by the invention comprises the following steps of: quickly breaking the dormancy of seeds through soaking the seeds into gibberellin, and carrying out seed stratification in a hotbed three months ahead of schedule; cutting the taproot growing points of sprout-stocks so as to carry out sprout-stock root breaking; carrying out container seedling-raising by using customized containers and a self-made matrix, placing the customized containers in a greenhouse and keeping for half a month, and after the customized containers are moved out of the greenhouse, completely burying the containers into soil; during the fast growing period of stock seedlings, daubing a gibberellin solution on the basal parts of stems, so that the ground-diameter thickening is effectively promoted, and a large number of high-quality stock seedlings meeting the requirements of grafting are raised in early July; and about 10-15 days after the grafting is successful, cutting out stock parts 1cm above graft buds, daubing gibberellin on the graft buds, and strengthening nutrient and water management, so that the graft buds grow to branches of 30-40 cm, and the heights of grafted seedlings in the current year meet the out-of-nursery standards of 50-60 cm, thereby raising a large number of healthily-growing 'three-current' well-bred seedlings of Carya illinoensis. Compared with the traditional seedling raising method, two years are shortened.

The invention relates to an edible fungi culture medium, in particular to a medium for growing pleurotus eryngii by using grape branches. The medium for growing the pleurotus eryngii by using the grape branches is characterized in that: each 100 weight parts of culture medium comprises 63.89 to 79.69 weight parts of grape branches, 15.27 to 26.66 weight parts of bran, 3 to 8.16 weight parts of bean pulp and 3 to 6.85 weight parts of corn meal; and each 100g of culture medium comprises 68.03g of grape branches, 20.41g of bran, 8.16g of bean pulp and 3.40g of corn meal. The grape branches are taken as a main material and properly added with other biological nitrogen sources to form the medium for producing wooddecay fungi; and the culture medium can degrade cellulose, hemicelluloses and lignin in the grape branches, and produce high-quality edible fungi.

The invention belongs to the technical field of plant propagation, in particular to a Chinese rose or rose rapid propagation method by utilizing a non-thorn multiflora for no-root grafting. The method includes 1, shearing a branch of an annual non-thorn multiflora, cutting the branch with the length of 15 to 20 cm, and keeping 1 to 2 buds to serve as grafting rootstocks; 2, selecting an annual or less annual Chinese rose or rose to serve as scions, cutting the Chinese rose or rose with the length of 10 to 15 cm, and keep 1 to 2 buds; 3, grafting the scions to the rootstocks by the grafting method, tying through a plastic film tightly, and cutting cuttings into growing matrix; 4, shielding, moisturizing and preventing the cuttings from freezing injury; when buds sprout in the next year, keeping the buds sprouted on the scions, and removing the buds sprouted on the rootstocks; 5, transplanting obtained cutting seedlings to the field after 45 d. By the aid of the method, survival rate is high, growth potential of the seedlings is strong, and the seedlings can be propagated annually.

The invention discloses a method for cultivating grapes yielding twice per year. The method specifically includes the steps of trimming short branches in the first ten days and the middle ten days of January, and covering a greenhouse to raise the temperature half a month after trimming; spraying a 2% hydrogen cyanamide solution for accelerating germination after the temperature per day in the greenhouse reaches 10 DEG C; cultivating the grapes instead in a rain-shielding mode in the middle ten days of May; dressing a magnesium and potassium mixed fertilizer on the first batch of fruits once in the expansion period, wherein the first batch of fruits ripen in July and August; conducting secondary trimming half a month after the first batch of fruits are harvested, reserving four to six buds at the base part of each mother branch, and dispersing and removing leaves; smearing two buds at the top end of each branch with a 2% hydrogen cyanamide solution for accelerating germination after trimming; applying an organic fertilizer once before the second batch of fruits sprout, wherein the amount of the consumed organic fertilizer accounts for one third the total amount of consumed fertilizers in the whole year; dressing a magnesium and potassium mixed fertilizer on the second batch of fruits once in the expansion period, covering the greenhouse for preserving the heat in the last ten days of October and the first ten days of November, wherein the second batch of fruits ripen before and after the New Year Day. By means of the method, the yield of the grapes is effectively increased by about 30% to 50%, quality is improved, the supply period of fresh grapes is adjusted, and economic benefits of grape plantation in regions in the south of Yangtze River are remarkably improved.

The invention discloses a citrus scion pretreatment technology for promoting removal of composite infection pathogens. The citrus scion pretreatment technology includes acquiring citrus branches, pruning the citrus branches to remove leaves and leaf stalks and disinfecting the surfaces of the citrus branches by the aid of sodium hypochlorite (NaClO) aqueous solution; detecting molecules of scions of the citrus branches with the disinfected surfaces; performing detoxification treatment on scion materials by the aid of insecticides if the detected citrus branches are infected by HLB (huanglongbing) viruses and CTV (citrus tristeza viruses), acquiring virus-free shoots and completing the citrus scion pretreatment detoxification step. The citrus scion pretreatment technology has the advantages that the citrus branches are treated by the aid of small-molecule bactericide triclosan (TCL) and a plurality of antibiotics, so that virus-free stem tip materials without the citrus tristeza viruses (CTV) and huanglongbing (HLB) pathogens can be acquired; the detoxification efficiency is high, and a good effect can be realized; the virus-free stem tip materials can be provided for further stem tip micro-shoot grafting or tender-shoot grafting.

The invention relates to a method for batch preparing golden leaf elm Caoshu dish garden, comprising the following steps: sowing with annual white elm, shaping with iron wire un-intertwining method, producing mass Caoshu shape tree trunk, cultivating in the soil for more than one year, intercepting the white elm trunk in position 5-10 cm from the Caoshu character in June to August of the second year, grafting the golden leaf elm onto the new growing branch of the white elm with bud grafting method after new branch growing out, making the survival golden leaf elm form into golden leaf elm new tree crown, making the tree crown of the golden leaf elm become cloud slice shape with 1-5 layers through brown method or intertwining with metal wire, then cultivating it in basin for shaping. The invention is characterized in that: the tree species for dish garden is colorful golden leaf elm, which shortens the producing cycle and is good for mass production.

The invention discloses a cultivation method of harvesting south grapes twice a year. The cultivation method comprises the following specific operations of trimming the grapes in mid-to-late January, carrying out bud forcing treatment when the temperature is stabilized to be more than 10 DEG C in late February and mid-March, spraying a tip-control inhibitor and pinching to promote blade ageing and flower bud differentiation, blooming in April and May, applying a first compound fertilizer after first fruits are fully matured; trimming the grapes in June and July to force winter buds to germinate, harvesting the first fruits in July and August, causing branches to bloom to obtain second fruits, applying a second compound fertilizer, and harvesting the second fruits in October and November. Through the scheme, the grapes can be harvested twice a year, so that the yield of the grapes is effectively improved by about 10%-20%, the fresh grape supply period is prolonged, and the economic benefit for planting the south grapes is remarkably improved.

The invention discloses a tree form control-based cherry culturing method and belongs to the field of fruit growing. The method comprises the following steps: planting a cherry seedling, controlling the stem height before the seedling sprouts in the first year, and maintaining 10 to 12 main branches on the central stem; in the spring of the second year, cutting exuberant stems at the root of the central stem, bending branches in spring and autumn, and increasing fertilizer; in the third year, pinching two-year-old young sprouts and side branches, and culturing fruiting branch sections; and in the fourth year, continuously pinching the young sprouts and the side branches, and performing open-center on the central stem when the central stem reaches a preset height. According to the method, the central stem and more main branches are bent and trimmed, side branches are removed, and the bent main branch is used as a fruiting branch section and is open-center molded in the fourth year. According to the method, overlap among branches can be effectively prevented, the crown can be prevented from over luxuriant by removing side branches, bore lighting can be improved by open-center, and the fruiting rate and fruit quality of a cherry tree can be improved.

The invention relates to a method for rapid propagation and timely flowering of China rose, and relates to the technical field of flower planting. The method comprises the following steps: in January and February, scissoring annual stingless wild rose branches, and reserving one or two bud points to be taken as stocks for grafting; selecting annual China rose branches as scions, reserving one or two bud joints, cutting the upper ends of the scions to be flat, cutting the lower ends of the scions to be inclined planes with the angles of 30-45 degrees, wherein the distances between pruning wounds and axillary buds are 1 cm; putting the cut stocks and scions in nutrient solution and soaking for 10-20 minutes, then grafting the soaked stocks with the scions together, tightening grafting ports with plastic films, and then daubing rooting powder on the roots of the stocks and inserting the roots of the stocks in soil; beginning to transplant two months later after the stocks are alive through cottage, irrigating and fertilizing according to a conventional management method 15-20 days later, wherein the China rose can be flowered in May. The method is simple and feasible; compared with a traditional grafting method, the survival rate can reach more than 90%, nursery stocks grow strongly, the China rose can be flowered timely, the planting cost is reduced, and the economic benefit is improved.

The invention provides a 'propagation method for the hard wood cutting of 'golden common vetch''. The propagation method is characterized by comprising the following steps of: selecting the annual 'golden common vetch' branches free of plant diseases and insect pests and with the coarseness within the range of 0.2-0.6cm in March, shearing into cutting slips with the lengths of 5-10cm, and soaking in water for 12-24 hours; dipping the roots of the cutting slips at 1.5-3.0cm for 10-20s by 75% ethyl alcohol, washing by clean water for 2-3s, inserting into 800-1000mg.L<-1> IBA (iso butyl alcohol) solution for 30-50s, and inserting into a matrix mixed by mire soil and perlite according to the proportion of 1:1, wherein the cottage depth is 1/2 of the length of the cutting slips, and one golden common vetch is inserted into every one hole disc; spraying water after cottage, so that the branches are tightly contacted with the matrix; and entering a seedling exercising period when the rooting percentage of the cutting slips reaches up to 60-70% of a sword, and planting when the seedling exercising time is 10-15d. The survival percentage of the cutting slips of 'golden common vetch' propagated by the method disclosed by the invention can reach to be more than 80% of the sword under the condition of a fog system, and the method is ordered in nursery stock, enough in delayed effect, and extremely good in commodity.

The invention relates to a high-yield mulberry cultivation method. A local 'Qinzhou mulberry' variety in Guangxi province serves as a rootstock and the 46CO19 bred in Taiwan serves as a scion. The cultivation method includes soil preparation, seedling culture and grafting, nursery stock processing, planting, pruning and cutting, field management, pest control, mulberry management in blossom and fruiting periods and the like. The mulberries cultivated through the method are developed in root system, cultivate in moisture and multiple in branches and fruits, the sugar content is 9.2-14.5%, the acidity is 4.5-6g/L, the pH is 3.7-4.4, the juice yield is 72-78%, the mulberries can be eaten freshly and processed, can cultivate silkworms after fruit picking, and are suitable for being planted in barren soil in mountain areas in Guangxi province to improve restore ecological environments and increase incomes of mass peasants in poverty-stricken areas.

The invention discloses a method for producing cattle and sheep complete feed with paper mulberry branches and leaves as a raw material through secondary fermentation. The feed is prepared from, by weight, 30-55 parts of paper mulberry branches and leaves, 25-40 parts of plant material, 8-10 parts of corn flour, 5-8 parts of rice bran, 3-5 parts of soybean meal, 3-4 parts of sweet potatoes, 0.1-1 part of fermentation strain and 0.1-0.3 part of microelement. The paper mulberry branches and leaves are fresh leaves and tender branches cut from paper mulberry; the plant material comprises one or a mixture of sugarcane tail ends, corn straw, fresh pasture, wheat straw, straw, paper mulberry, peanut vine and weeds. The cattle and sheep feed prepared from the paper mulberry branches and leaves through fermentation is simple in manufacturing method, low in cost, has comprehensive nutrition, is fragrant and sweet and improves cattle and sheep appetite, and cattle and sheep like to eat the feed and grow fast.

The invention discloses a high-vertical-rack grape cultivation method, which comprises the following steps that after young shoots grow out of a grape seedling, a robust shoot is selected, guided and tied on a vertical bar and is used as a trunk to cultivate, and when the trunk grows to an extent exceeding a bearing line, the top end of the trunk is removed and pinched; side shoots below pinchingpositions are selected as a main vine to cultivate along the direction of the bearing line and are tied on the bearing line; medium and short shoot branch groups are cultivated on the two sides of the main vine in a staggered way, buds on the branch groups are cultivated as bearing branches or foliage branches, the foliage branches and preparatory branches are not specially cultivated, all bearing branches and foliage branches on the branch groups are not artificially tied and bearing branches which bear fruits in the same year are used as permanent bearing branches to cultivate; and when theyoung shoots of the medium and short shoot branch groups are semi-lignified, the young shoots are kept to grow horizontally or obliquely downwards through artificial shoot twisting. Compared with theexisting grape cultivation rack cultivation method, the high-vertical-rack grape cultivation method has the advantages that more labor and line materials are saved, the mechanical pruning is facilitated, the growth vigor is naturally alleviated, the development of branches is good, the quality of fruits is high, the high yield and the stable yield are facilitated and the popularization and the implementation are facilitated.

The invention discloses a clear type sea-buckthorn fruit drink and a production method of the clear type sea-buckthorn fruit drink. The production method is characterized by comprising the following steps of: taking sea-buckthorn fruit with branch as the raw material; preprocessing; juicing; carrying out enzymolysis and debitterizing; coarsely filtering; deactivating enzyme; fine filtering; blending; sterilizing; filling and sealing the port; inverting bottle and sterilizing; cooling; sleeving a label; spraying the code; packing; and thus obtaining the clear type sea-buckthorn fruit drink which remains the unique flavor of the sea-buckthorn fruit and tastes sweet and sour. The sea-buckthorn fruit drink processed by the production method disclosed by the invention is bright in color and luster and free from bitterness of tannin, and effectively remains the taste and flavor of the sea-buckthorn fruit.

The invention discloses a cuttage propagation method for styrax tonkinensis. The cuttage propagation method has the characteristics of being low in cost, simple and convenient to operate, good in effect, simple, feasible, economical and practical. The cuttage propagation method comprises the following steps of: (1) mixing perlite and turf to form a cuttage matrix; (2) using semi-lignified shoots on a seed tree as cutting shoots, wherein the cutting shoots are 10-15cm in length, and each cutting shoot is provided with three or more buds; (3) promoting the growth of the roots of the cutting shoots by a plant growth hormone; (4) carrying out cuttage, wherein 1/2-2/3 of the length of each cutting shoot is embedded into the cuttage matrix; (5) when the cuttage is finished, thoroughly watering so that the cutting shoots are in full contact with the matrix; and (6) rooting and transplanting, wherein the shoots can be transplanted after being subjected to cuttage for three months.

The invention relates to a method for decomposing and fermenting garden dry branches and fallen leaves into an organic fertilizer, aiming at piling and fermenting the dry branches and fallen leaves to be converted into the organic fertilizer. The method comprises the following steps: collecting the dry branches and fallen leaves of garden plants from thick green areas in autumn, such as a road green space and a park; cutting and crushing the collected dry branches; mixing with the collected fallen leaves; adding raw cattle excrements to the mixture of the dry branches and fallen leaves in order to enhance the fertility of the organic fertilizer; mechanically agitating; uniformly mixing; adding a fermentation bacteria agent; and finally piling, decomposing and fermenting. By adopting the organic fertilizer production method, the waste in a garden can be converted into the organic fertilizer; moreover, the cost is low, the operation is simple, the prepared organic fertilizer is high in nutrient content, the physicochemical properties of soil can be improved well, and the pollution of the garden waste to environment can be reduced effectively.

The invention discloses a method for propagating Taxus madia nursery stocks. The method comprises the steps of (1) preparing a sterile fine-sand seed bed and setting up a shading shed; (2) mixing turf with perlite in the proportion of 6:4 to prepare matrix nutrition cups; (3) matrix nutrition cups are evenly spaced and placed on the seed bed and sterilized fully; (4) selecting branches of Taxus madia plants of three to four year olds in spring or in autumn for cuttage in nutrition cups; and (5) keeping the temperature of the seed bed to be within a range of 18-30 DEG C and the humidity to be 65-70%, spraying foliage fertilizer for the first time after 9-10 days, spraying the foliage fertilizer once every 7-10 days during the fast-growing period of nursery stocks, cutting roots by air or breaking roots through movement of nutrition cups, stopping spraying the foliage fertilizer when most of roots grow, and then hardening seedlings till the next spring to conduct large field transplantation. According to the method, propagated Taxus madia nursery stock roots are balanced, neat and robust, the rate of survival exceed 96%, the seedling rate exceed 95%, the survival rate of transplanting can achieve 98%, and industrialized production can be achieved.

The invention relates to a production method of a sweet osmanthus tree vase, comprising the following steps: S1, branch selection and cottage; S2, branches transplanting: selecting flourishing and strong sweet osmanthus tree seedlings, and then carrying out determination and planting on the seeldings; S3, seedling cultivation; S4, knitting and modeling: selecting seedlings with the same height and size from the cultured sweet osmanthus tree seedlings, ensuring multiple strains of the sweet osmanthus tree seedlings to be distributed and arranged according to circumference with a certain number so as to form a cluster of the sweet osmanthus trees, managing and cultivating the cluster of the sweet osmanthus trees to let the sweet osmanthus tree seedlings grow and survive; and after the sweet osmanthus tree grows and survives, knitting and modeling the setting mould of the sweet osmanthus tree to enable the branches of a plurality of the sweet osmanthus tree seedlings to be curved and enveloped into a vase structure, and carrying out manual treatment on the trunk crossing section of the sweet osmanthus tree to ensure the natural growth and coalescence of the trunks so as to be connected together. The invention has the beneficial effects of enabling the joint of the sweet osmanthus tree to naturally heal and grow into a whole, thus the sweet osmanthus tree vase formed in the way is firmer.

The invention discloses a clonal cutting method of camellia arborescens seedling culture. The clonal cutting method comprises the steps of seed selecting, seedbed building, cutting, shed building for shading and film covering for thermal insulation. According to the method, the rate of survival of branches is 98%, more camellia arborescens seedlings can be planted continuously and cultured into camellia arborescen black teas like gold fir tea. The gold fir tea of the camellia arborescen black teas has a plurality of effects such as thirst quenching, summer-heat relieving, digestion helping, distention eliminating, detoxifying, detumescence, refreshing, intelligence reinforcing, eyesight improving, stomach invigorating, stasis dissipating, diarrhea checking and hiccup checking.

The invention discloses a method for planting salvia officinalis in hardness adherance, which belongs to the technical field of algae propagation. The method comprises the following steps of: pre-processing the adherance, preparing a seedling collection pond, preparing to plant algae, collecting seedlings, cultivating young seedlings, putting in a sea area and managing on the sea. According to the method disclosed by the invention, the salvia officinalis are directly planted on artificial reefs, stones or cement components, can be firmly combined on the surfaces of the artificial reefs, the stones or the cement components through adhesion accentuation trainings in stages and then can be put into sea water in a appropriate time, new branches of the salvia officinalis can be grown on the adherance year by year, the problems that the salvia officinalis are in resource exhaustion due to man-made damage and the year-to-year propagation for maintaining algae resources in the sea area are solved, the method plays a great role in environment modification, resource recovery and the like, and simultaneously, the operation is simple and convenient.

The invention discloses a cuttage propagation method for anisetree bark. The method comprises the following steps: a, selection and treatment of cutting slips: selecting robust branches, cutting into cutting slips, soaking the base parts of the cutting slips into disinfecting liquid for 30 minutes, and soaking in a rooting inductive agent for 2 hours; b, preparation of a substrate and a seedbed: selecting a uniform mixture of peat soil, perlite, vermiculite and fine river sand for serving as a cutting substrate, disinfecting the cutting substrate with disinfecting liquid, and making a seedbed of 15 centimeters in thickness; c, cottage, obliquely cutting the cutting slips into the seedbed substrate by 7-10 centimeters; d, management of the seedbed after cuttage: spraying water onto the seedbed in the morning and in the evening each day, spraying 500-time thiophanate methyl seven days later once every week for two times in total, spraying 3/4MS nutrient liquid one week later, and culturing till rooting and seedling emergence. By adopting the cuttage propagation method in breeding of anisetree bark seedlings, the breeding speed of the anisetree bark is increased, and the seedling breeding and scale planting of the anisetree bark are ensured greatly.