65results about How to "High detoxification efficiency" patented technology

The invention discloses a mycotoxin detoxification agent for feed and a preparation method and a feed additive thereof, and belongs to the field of feed additives. The mycotoxin detoxification agent can be used for detoxification of feed raw materials and compound feed of mycotoxin polluted corn, grains and the like. The mycotoxin detoxification agent consists of modified montmorillonite, yeast cell wall, chitosan and the like; and the preparation method has simple process. The novel mycotoxin detoxification agent for feed can effectively remove pollution of multiple mycotoxins such as aflatoxin, zearalenone, T2 toxin, vomitoxin and the like in the feed raw materials and the feed of the corn, the grains and the like, and prevent various diseases of livestock and poultry because of eating the mycotoxin polluted feed. The mycotoxin detoxification agent has simple preparation process, stable and controllable quality, convenience in use and low price, meanwhile has the advantages of supplementing nutrients, improving the disease resistance of cultured animal bodies and avoiding adsorbing nutrient elements in the feed, and guarantees the health and safety of the cultured animals.

The method includes following steps: to filter and clear alge in water by sand to reduce quantity of suspended substances and microcap frustule in water; next is plant absorbing process, a section of organic substances and microcystim are absorbed and decomposed by sandy soil plate which hygrophytes are planted on; the third is to further get rid of suspended substances and microcap frustule in water by active carbon adsorbing installation; the fourth is ozone process by blowing in high-density ozone containing air; the fifth is to detect content of microcystim in drinking water processed by method of high-effective liquid phase chromtograph detection.

The invention relates to the field of enzyme engineering, and in particular discloses acid-resistant zearalenone detoxifying enzyme and an encoding gene thereof, and application of the enzyme and the gene. The amino acid sequence of the zearalenone detoxifying enzyme is optimized, 8 amino acid residues (with 8 amino acid difference from zlhy-6 detoxifying enzyme) of the detoxifying enzyme are changed, the optimal reaction pH of recombinant expression zearalenone detoxifying enzyme is reduced from 8.5 to 6.5 and is very identical with the pH of pig and chicken intestinal canals, and the enzyme activity of the detoxifying enzyme still maintains 40 percent or more when the pH is reduced to 5.0, so the detoxifying efficiency of the zearalenone detoxifying enzyme serving as a feed additive to actual application is greatly improved. The zearalenone detoxifying enzyme gene is subjected to codon optimization, and the copy number of the gene in a recombinant yeast genome is increased to be 4, so the expression quantity of the zearalenone detoxifying enzyme is greatly increased.

The invention relates to 'a method for removing strawberry mild yellow edge virus (SMYEV) by the ultralow temperature technique', which belongs to the field of bioengineering. During the virus removal process, the concentration of pre-cultured sucrose is 0.5 mol per liter, and 3d is treated; the 3d is subjected to loading treatment for 60 minutes at a temperature of 25 DEG C; the 3d is subjected to vitrification treatment for 120 minutes at a temperature of 0 DEG C; and the 3d is subjected to liquid nitrogen treatment for 60 minutes and water bath treatment for 2 minutes at a temperature of 40 DEG C, wherein the survival rate of strawberry stem tips is 76 percent, and the detoxication rate of the strawberry mild yellow edge virus reaches 95 percent. The virus can be only removed by means of the liquid nitrogen treatment, and the detoxication rate of the vitrification treatment before the liquid nitrogen treatment is 0 percent. Compared with the prior detoxication method such as the stem tip culture detoxication method, the heat treatment detoxication method, and so on, the ultralow temperature detoxication method not only has quite high detoxication rate, but also is simple and feasible, convenient to operate, and does not require expensive instruments, so as to provide strong technical support for demonstration and popularization and industrialization of nontoxic strawberry seedlings.

The method for producing detoxified seed seedling by combining garlic shoot tip culture with cold treatment includes the following several steps: (1), cold storage treatment of garlic hop; (2), selecting material and sterilization; (3), shoot tip culture; (4), successive transfer culture; (5), test-tube plantlet rooting; and (6), training plantlet and transplating. Said invention also provides three cuture media for above-mentioned shoot tip culture, successive transfer culture and rooting culture.

The invention belongs to the technical field of the plant tissue culture, and particularly relates to a cultivating method for acquiring a Zengcheng honey chrysanthemum virus-free seedling through micro-stem tip culture combined with heat treatment. The provided cultivating method for acquiring the Zengcheng honey chrysanthemum virus-free seedling through the micro-stem tip culture combined with the heat treatment comprises the following steps: breeding a good strain, establishing a tissue culture sterile tissue culture vessel seedling, pre-eradicating viruses by heat treatment culture, eradicating viruses by the micro-stem tip culture, establishing a virus-free stem tip tissue culture sterile breeding system, detecting a virus-free stem tip tissue culture seedling virus and industrially growing the seedling through virus-free seedling tissue culture and the like. The provided method for acquiring the Zengcheng honey chrysanthemum virus-free seedling is capable of using the micro-stemtip tissue culture combined with heat treatment virus eradication, and cooperating with an improved culture medium, is high in virus-free seedling survival rate, rapid in breeding speed, and capable of industrially breeding the growing the seedlings through the large-scale tissue culture, and high in economic benefit.

The invention discloses a detoxification method of cottonseed cake or cotton seed meal, which is a method for grinding and detoxifying the cottonseed cake or the cotton seed meal containing free gossypol in a high-energy grinder under the effect of the grinding auxiliary agent, and comprises the following steps that: 1) the cottonseed cake or the cotton seed meal containing the free gossypol is measured, and then the grinding auxiliary agent with the weight being 1 to 10 percent of that of the cottonseed cake or the cotton seed meal is measured to be uniformly mixed with the cottonseed cake or the cotton seed meal; 2) the above mixture is added into the high-energy grinder to be ground for 5 to 60 minutes so as to obtain the detoxified cottonseed cake or the detoxified cotton seed meal. The method overcomes the weaknesses of the traditional physical and chemical detoxification technology that the high temperature and high-moisture treatment is required, and the energy consumption is high, has simple process, less equipment investment, convenient operation, high detoxification efficiency, is free from three wastes during the production process, and is applicable to the large-scale industry. The prepared detoxified cottonseed cake or the detoxified cotton seed meal has good palatability, high digestibility and low content of anti-nutrition factor.

The invention discloses a method for using a rimantadine hydrochloride drug to culture lily under a virus-free state and belongs to the fields of plant tissue culturing fast propagation technique and biologic technique. The method comprises the following steps of: inspecting lily mother bulb virus, preparing a culture medium and reserving, sterilizing and inoculating explants, culturing the explants, inspecting virus of tissue culturing seedlings, and the like, thereby quickly obtaining lots of tissue culturing seedlings. According to an orthogonal method, a formula of the best induced culture medium of buds and root-less tissue culturing seedlings directly differentiated from lily is obtained; a rimantadine hydrochloride antiviral drug with a series of concentration gradients is prepared and an optimum detoxifying concentration is selected, so that the detoxifying efficiency can reach 93%; a period of induced culturing is shortened, and meanwhile, the detoxifying efficiency is high and the enhancement factor is high; and a technical basis for researching on the culturing for lily virus-free plants and the popularization for virus-free culturing is provided.

The invention relates to the technical field of plant tissue culture, and provides a method for detoxifying culture of anthurium andraeanum to solve the problems that a detoxified tissue culture seedling is high in operation difficulty, low in detoxification efficiency, long in production cycle and the like in production. The method comprises the following steps: explant preparation, induced culture, enrichment culture, variable-temperature heat treatment, differentiation culture, induced culture of a quasi detoxification stock plant, proliferation and differentiation of the quasi detoxification stock plant, growth of a quasi detoxification strain, virus detection, proliferation and differentiation of detoxification materials, and rooting and transplantation of detoxified seedlings. The method for detoxifying culture of the anthurium andraeanum is simple and convenient to operate, high in detoxification material yield and high in speed.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a blueberry tissue culture seedling detoxification method and culture method. The detoxification method comprises the following steps: (1) heat treatment: treating a blueberry tissue culture seedling at 35-50 DEG C for 3-3.5 days; and (2) chemical agent treatment: cutting off a stem apex of the tissue culture seedling treated by the step (1), putting the stem apex in a virazole-containing culture medium, and continuing culture at 21-25 DEG C for 45-55 days, wherein the concentration of the virazole is 0.2-0.3 mg/L. On such basis, the invention also provides a blueberry tissue culture seedling culture method containing the detoxification method. According to the method, the two-stage detoxification treatment is performed to greatly enhance the detoxification rate of the blueberry tissue culture seedling, and the survival rate of the tissue culture seedling is basically not influenced. The detoxified blueberry seedling can not mutate after being transplanted to the field. Therefore, the methods provided by the invention have broad market prospects and important application and popularization values.

The invention discloses a method for detoxicating chrome leaching residues through a sintering technology. The method comprises the steps that the chrome residues are screened and fully mixed with water-quenched slag and coke powder through a blender mixer, the mixture is prepared into particles by adding water, the particles are uniformly distributed on a sintering trolley, the effect that the mixture particles are spread flatly and completely distributed on the sintering trolley to be sintered is ensured, and the ignition temperature is controlled to be 1100-1200 DEG C; the negative pressure of a sintering wind box is controlled to be 12-13 kpa; the vertical sintering speed is less than 18 mm/min; high-temperature finished product sintered ore is subjected to air blast cooling; and screening is conducted, and finished product sintered ore is obtained. The method has the advantages that the process is reasonable, the production energy consumption is low, the cost is reduced, harmless treatment of the chrome residues is achieved, and the detoxication efficiency is high; and the index of Cr6+ in a water leaching sample is lower than the national standard, and the method is suitable for industrial large scale treatment.

The invention provides a coating for adsorbing and inactivating viruses. The coating comprises porous material powder, polyacrylate, hydroxypropyl methyl cellulose, polyethylene glycol and water, a shell of a porous material particle is composed of an oxygen storage material SiO2-CeO2, and a core of the porous material particle is a hierarchical pore molecular sieve. The coating disclosed by the invention can be used for adsorbing and inactivating viruses, and further, the coating is coated on an indoor wall surface and is used for purifying air in large public places of closed spaces such as hospitals, civil aviation, high-speed rails, subways, buses, office buildings and the like.

The invention discloses a method and device for continuously treating heavy metal polluted sludge, belongs to the field of solid waste treatment. The method comprises the following steps: S1, closinga cathode chamber and the like, and opening an underwater electromagnetic valve and the like; S2, turning on an ultrasonic generator; S3, switching on a power supply, and starting electrolysis; S4, reacting for precipitation; S5, performing in a filtering chamber; S6, starting a first sewage pump to start feeding and discharging; S7, opening a gas valve to release hydrogen; S8, closing the underwater electromagnetic valve, a third sewage pump, a leaching valve and a drainage valve, and opening a second sewage pump and a backwashing valve; and S9, closing the backwashing valve, and opening thedrainage valve. Heavy metals adsorbed and wrapped by sludge can be effectively treated, sludge odor can be removed, and sludge harmless treatment is achieved; metals such as copper and zinc in the sludge are recycled; and harmless treatment of the heavy metal polluted sludge can be realized without additional chemicals.

The invention discloses a cottonseed-meal detoxification method. The cottonseed-meal detoxification method includes the following steps that 1, cottonseed meal subjected to oil expression is put into a tea fixing machine and parched for 4 minutes to 6 minutes; 2, dried cottonseed meal is put into a nylon ball-milling tank, and under the condition of the rotating speed of 140 turns/min, the dried cottonseed meal is continuously subjected to ball milling for 2 hours, and cottonseed-meal powder is obtained; 3, the cottonseed-meal powder is wetted till the water content is 40% to 42%, and a wetted material is obtained, sent to a microwave treating device and subjected to interval microwave treating under the condition that the microwave frequency is 2,450 MHz, and the power is 500 W; 4, the cottonseed-meal powder subjected to microwave treating is sent to a stirrer, zymophyte liquid with the weight accounting for 0.5%-1% of that of cottonseed-meal powder subjected to low-temperature cold-air drying, cortex dictamni extract with the weight accounting for 0.2% of that of cottonseed-meal powder subjected to low-temperature cold-air drying, salt with the weight accounting for 0.5% of that of cottonseed-meal powder subjected to low-temperature cold-air drying, zinc sulfate with the weight accounting for 0.02% of that of cottonseed-meal powder subjected to low-temperature cold-air drying, baijiu with the weight accounting for 0.6% of that of cottonseed-meal powder subjected to low-temperature cold-air drying and corn peptide with the weight accounting for 0.05% of that of cottonseed-meal powder subjected to low-temperature cold-air drying are added into the stirrer at the same time, the mixture is continuously stirred and mixed for 50 minutes or above, and standing is carried out for 3 days to 5 days; 5, the stood powder is taken out and put into a drying device, the temperature is 90 DEG C to 95 DEG C, baking is carried out till the water content of the powder is lower than 15%, and detoxification is completed.

The invention discloses a method for acquiring a zinnia elegan virus-free seedling. The method comprises the following steps: (1) collecting zinnia elegan tender leaves, and carrying out the dedifferentiation to obtain callus; (2) thermally treating the callus, then continuously inducing the callus to have an adventitious bud, and carrying out rooting culture for the adventitious bud to obtain tissue culture seedling; (3) further stripping a stem tip of the tissue culture seedling, and carrying out the dedifferentiation to obtain callus; (4) carrying out the thermal treatment on the callus again, and inducing to form a seedling; (5) randomly extracting the tissue culture seedling in the step (4), carrying out the virus detection, if the seedling is qualified in the detection, determining the seedling to be the zinnia elegan virus-free seedling, if the seedling is unqualified, repeating the steps (1) to (4) until the qualified zinnia elegan virus-free seedling is acquired. The callus is generated by virtue of twice dedifferentiation, then differentiation is carried out after the thermal treatment, the embryonic callus induction and stem tip induction detoxification are integrated, the final zinnia elegan virus-free seedling is obtained, so that the difficulty that the existing detoxification technology is non-thorough in detoxification and complicated in operation can be solved, and the factory-like detoxification production requirement can be met.

The invention discloses an efficient lily detoxification method which comprises the following steps: 1) selecting healthy and non-rotten lily scales to extract RNA, and carrying out virus detection through reverse transcription; 2) selecting lily scales with healthy middle and inner layers for explant disinfection, and culturing to obtain a large number of aseptic seedlings with good growth vigor;3) cutting off small scales of the aseptic seedlings, and inducing to obtain compact calluses; 4) performing differentiation culture on the calluses and inducing the calluses into seedlings; and 5) cutting the leaves in the step 4) for virus detection, wherein the lily virus-free seedlings are qualified. According to the lily detoxification method, the non-toxic seedlings are rapidly obtained byinducing the calluses, the problems that an existing lily detoxification process is complex, high in technical requirement and long in consumed time are solved, the lily detoxification method has theadvantages of being high in detoxification efficiency, simple in process and low in production equipment requirement, and the industrial production requirement can be met.