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Method for detoxifying culture of anthurium andraeanum

An anthurium and culture medium technology, applied in the field of plant tissue culture, can solve the problems of difficult operation, low detoxification efficiency, long production cycle and the like, and achieve the effects of low pollution rate, shortened production cycle and easy operation.

Active Publication Date: 2015-04-29
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of difficult operation, low detoxification efficiency and long production cycle in the production of anthurium detoxification tissue culture seedlings, the present invention proposes a method for anthurium detoxification cultivation, which is a simple and easy-to-operate, detoxification Virus-free culture method for anthurium seedlings with high material acquisition rate and fast speed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take the young leaves and petioles newly exhibited by Anthurium Alabama as explants, which are sterilized: rinse with running water for 30 minutes, then soak with 70% alcohol for 1.0 minute, rinse with sterile water, and then use 0.1% liter Soak in mercury solution for 8 minutes, then rinse with sterile water 5 times. Then the explants were sliced ​​and sectioned and inoculated on the induction medium MS+6-BA 1.0 mg / L+2.4-D 0.5 mg / L. After 60 days, yellow granular callus tissue can be seen on the explants. Inoculate the rice grain-sized callus with explants on the proliferation medium MS+6-BA 2.0mg / L. After 80 days of proliferation and culture, there are several callus clumps with a diameter of 0.5-2 cm and differentiation on the surface. little buds. The small buds were removed, and the callus was placed in an artificial climate box at 38°C for 8 hours, and then placed in an artificial climate box at 28°C for 16 hours, and the cycle was like this for 30 days. After ...

Embodiment 2

[0031] Take the young leaves and petioles newly exhibited by Anthurium Alabama as explants, and undergo sterilization treatment: first rinse with running water for 30 minutes, then soak with 70% alcohol for 0.5 minutes, rinse with sterile water, and then use 0.1% liter Soak in mercury solution for 10 minutes, then rinse with sterile water 7 times. Then the explants were sliced ​​and sectioned and inoculated on the induction medium MS+6-BA 1.0 mg / L+2.4-D 0.5 mg / L. After 60 days, yellow granular callus tissue can be seen on the explants. Inoculate the callus with the size of rice grains together with explants on the proliferation medium MS+6-BA 1.0mg / L. After 80 days of proliferation and culture, there are several callus masses with a diameter of 0.5 to 2 cm and differentiation on the surface little buds. The small buds were removed, and the callus was placed in an artificial climate box at 37°C for 8 hours, and then placed in an artificial climate box at 32°C for 16 hours, an...

Embodiment 3

[0033]Take the young leaves and petioles of Anthurium powder Champion Xinzhan as explants, after sterilization: wash with running water for 30 minutes, soak in 70% alcohol for 0.5 minutes, rinse with sterile water, and then use 0.1% mercuric chloride The solution was soaked for 10 minutes, and then washed 7 times with sterile water. Then the explants were sliced ​​and sectioned and inoculated on the induction medium MS+6-BA 1.0 mg / L+2.4-D 0.6 mg / L. After 80 days, yellow granular callus tissue can be seen on the explants. Inoculate the rice grain-sized callus with explants on the proliferation medium MS+6-BA 1.5mg / L. After 110 days of proliferation and culture, there are several callus clumps with a diameter of 0.5 to 2 cm and differentiation on the surface. little buds. The small buds were removed, and the callus was placed in an artificial climate box at 36°C for 12 hours, and then placed in an artificial climate box at 26°C for 12 hours, and the cycle was like this for 28 ...

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PUM

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Abstract

The invention relates to the technical field of plant tissue culture, and provides a method for detoxifying culture of anthurium andraeanum to solve the problems that a detoxified tissue culture seedling is high in operation difficulty, low in detoxification efficiency, long in production cycle and the like in production. The method comprises the following steps: explant preparation, induced culture, enrichment culture, variable-temperature heat treatment, differentiation culture, induced culture of a quasi detoxification stock plant, proliferation and differentiation of the quasi detoxification stock plant, growth of a quasi detoxification strain, virus detection, proliferation and differentiation of detoxification materials, and rooting and transplantation of detoxified seedlings. The method for detoxifying culture of the anthurium andraeanum is simple and convenient to operate, high in detoxification material yield and high in speed.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for virus-free cultivation of anthurium seedlings. Background technique [0002] Anthurium andraenum, also known as Anthurium and Anthurium, is a perennial herbaceous plant of the genus Anthurium in the family Araceae. In many tropical countries and regions, it is the main cut flower species next to tropical orchid, and it is also the main potted flower. After more than 20 years of development in my country's anthurium industry, it has also become the main potted flower and cut flower. However, with the expansion of production scale and the increase of planting years, the proportion of virus-infected plants in greenhouses and fields is also increasing year by year. Most of the seedlings produced on anthurium scale are tissue culture seedlings, but most of the domestic tissue culture seedlings are not virus-free seedlings. [0003] The key to the production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 潘晓韵田丹青葛亚英刘晓静潘刚敏
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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