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Method for acquiring zinnia elegan virus-free seedling

A method of obtaining virus-free seedlings, which is applied in the field of agricultural science, can solve the problems of cumbersome operation and incomplete detoxification, and achieve the effect of simple process, easy operation and simple operation

Inactive Publication Date: 2015-12-09
陈丁龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the deficiencies existing in the existing Zinnia detoxification technology, and provide a sustainable, easy-to-operate and large-scale production method for obtaining Zinnia detoxification seedlings. The problem of incomplete detoxification and cumbersome operation of detoxification technology can meet the needs of factory detoxification production

Method used

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  • Method for acquiring zinnia elegan virus-free seedling
  • Method for acquiring zinnia elegan virus-free seedling
  • Method for acquiring zinnia elegan virus-free seedling

Examples

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Embodiment 1

[0054] In 2012, the young leaves of Zinnia domestica variety Fangfei No. 2 were collected, detoxified according to the technical route of the present invention, and excellent virus-free seedlings were obtained. The detailed steps and results are as follows:

[0055] 1) Collect young leaves of Fangfei No. 2 and dedifferentiate to produce callus:

[0056] Collect the young leaves of the healthy and disease-free zinnia variety Fangfei No. 2 that have grown to the budding stage, wash them with tap water, rinse them with running water for 30 minutes, soak them in 70% alcohol for 15 seconds, add a few drops of Tween-20 solution to 0.1% mercury liter Soak for 15 minutes, stir with a glass rod, rinse 4-5 times with sterile water, blot dry with filter paper, punch holes with a hole puncher with a hole diameter of 4 mm, and inoculate the beaten leaves into callus induction medium (the formula of the medium is: MS+2.0mg / L6-BA+0.5mg / LNAA+0.3g / LCH+3% sucrose+6.0g / L agar, pH 5.8) cultured i...

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Abstract

The invention discloses a method for acquiring a zinnia elegan virus-free seedling. The method comprises the following steps: (1) collecting zinnia elegan tender leaves, and carrying out the dedifferentiation to obtain callus; (2) thermally treating the callus, then continuously inducing the callus to have an adventitious bud, and carrying out rooting culture for the adventitious bud to obtain tissue culture seedling; (3) further stripping a stem tip of the tissue culture seedling, and carrying out the dedifferentiation to obtain callus; (4) carrying out the thermal treatment on the callus again, and inducing to form a seedling; (5) randomly extracting the tissue culture seedling in the step (4), carrying out the virus detection, if the seedling is qualified in the detection, determining the seedling to be the zinnia elegan virus-free seedling, if the seedling is unqualified, repeating the steps (1) to (4) until the qualified zinnia elegan virus-free seedling is acquired. The callus is generated by virtue of twice dedifferentiation, then differentiation is carried out after the thermal treatment, the embryonic callus induction and stem tip induction detoxification are integrated, the final zinnia elegan virus-free seedling is obtained, so that the difficulty that the existing detoxification technology is non-thorough in detoxification and complicated in operation can be solved, and the factory-like detoxification production requirement can be met.

Description

technical field [0001] The present invention relates to a method for obtaining virus-free zinnia seedlings, in particular to a method for obtaining virus-free zinnia seedlings by generating callus tissue twice through dedifferentiation and heat-treating the callus tissue to obtain the final virus-free zinnia seedlings. The field of agricultural science and technology. Background technique [0002] Zinnia, Asteraceae, Zinnia genus, native to North America, Mexico and South America and other places, because of rising steadily, the flowers bloom more and more luxuriantly, and have won many good names, such as Zhuangyuanhong, Huoqiuhua, Zinnia, Backgammon and more. Zinnia likes warmth, is not cold-resistant, and is resistant to drought. It should be sunny, and the tongue-shaped flowers will increase under long-day conditions. Zinnia has a long flowering period and a large amount of flowers. Each plant blooms 30-40 flowers from the first flower to the frost. Most of the excell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 陈丁龙
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