The invention belongs to the field of potato stock seed culture and particularly relates to a method for culturing potato
virus-free test-tube plantlets in a water culture mode. The method comprises the steps of
seedling exercising, rooting treatment,
nutrition liquid preparation, field planting, culture and
transplanting. In aspects of variety, culture density,
nutrition liquid concentration and rooting agent concentration, the propagating factor and the strong
seedling quantity are increased, corresponding measures are adopted for handling in each link, the propagating factor of
virus-free seedlings is improved, healthy and strong
virus-free seedlings are obtained, and the soilless culture mode requirements of
fog culture, substrates and the like are met, so the healthy and efficient potato stock seed production is reached. The original virus-free, germless and
insect-free characteristics of the plantlets are maintained, the characteristics of simple technology, low cost, strong germchits and the like are also realized, in addition, the
survival rate of the water culture potato seedlings transplanted to the substrates or other soilless culture (such as
fog culture) is very high and can sometimes reach 99 percent, and the production efficiency of potato stock seeds is improved.