16 results about "Virus free" patented technology

The invention relates to the technical field of blueberry tissue culture and virus-free seedling culture, and particularly discloses an efficient culture method for blueberry virus-free tissue culture seedlings, which comprises the following steps: selecting and disinfecting explants: selecting virus-free blueberry stem tips as the explants; performing callus induction; bud differentiation culture: preparing a culture medium by adding ganoderma leukemia polysaccharide and the like into a WPM differential culture medium; rooting culture: preparing a culture medium, namely adding 0.8 mg / L of IBA, 0.2 mg / L of NAA, 20g / L of sucrose, 15mg / L of ganoderma leukemia polysaccharide, 7g / L of agar and the like into a WPM rooting culture medium. By adopting the method disclosed by the invention, the bud differentiation rate reaches 92.7%, no vitrification phenomenon occurs, the ganoderma leukemia polysaccharide regulates cell osmotic pressure and optimizes metabolic balance, the vitrification problem of tissue culture seedlings is solved, the seedlings are more robust, and the generation of malformed seedlings is reduced; the seedling stress resistance is enhanced, and the transplanting survival rate is increased.

The invention discloses a Tongling white ginger stem tip detoxification and propagation method which comprises the following steps: (1) disinfecting the surface of a Tongling white ginger tuber, separating bud points, and inoculating the bud points into an induction culture medium for induction culture of adventitious buds; (2) stripping stem tip meristem from the adventitious buds induced in the step (1), inoculating the stem tip meristem into a regeneration culture medium, and synchronously inducing buds and roots to obtain a complete regeneration plant; wherein the size of the stem tip meristem is 1-2 mm; and (3) selecting the completely detoxified Tongling white ginger regenerated plant obtained in the step (2) as a propagation material, taking the stem node as an explant, and inoculating the Tongling white ginger regenerated plant into a proliferation culture medium for sterile propagation to obtain Tongling white ginger seedlings. According to the method, a special efficient detoxification regeneration technology system for Tongling white ginger is established, virus-free, high-quality and healthy seedlings are provided for industrial cultivation of Tongling white ginger, and accurate protection and inheritance of excellent germplasm resources of national geographical indication products are achieved.

The invention relates to the field of bioengineering, in particular to a recombinant collagen which is characterized by comprising one or more of the following characteristics: a, an amino acid sequence of the collagen is shown as any one of SEQ ID NO.1-4; and b. A, the collagen is added, substituted or deleted with one or more amino acids, and the protein has collagen activity. The recombinant collagen prepared by utilizing a genetic engineering technology has the remarkable advantages of stable quality, no virus transmission risk and the like, and has a good application prospect in the field of medical materials.

The application provides a method for annual propagation of virus-free sweet potato seedlings by water culture, which comprises the following steps: washing the tissue culture seedlings detected free of virus and then soaking the seedlings in a treatment solution; transplanting the pretreated tissue culture seedlings to a seedling plate in a water tank, pouring the first nutrient solution into the water tank to promote the seedlings to rapidly root, and maintaining a certain temperature, humidity and light in the water culture room; replacing the nutrient solution in the water tank with the second nutrient solution after a certain period of growth, and regularly replacing the second nutrient solution; after the tissue culture seedlings grow into plants with a certain stem node, cutting the stem node segments, soaking the stem node segments in the treatment solution, and then repeating step (2) to obtain cutting seedlings, and spraying the plants with the growth-promoting solution to promote the growth of branches; repeating step (3) to culture the plants with the cut stem segments and the cutting seedlings, and regularly spraying the plants with the foliar nutrient solution and the insecticide. The propagation method is used for the propagation of virus-free seedlings by water culture, and the propagation of the seedlings is simple, efficient and easy to manage, and the annual and efficient propagation of the virus-free sweet potato seedlings can be realized.

The invention discloses a method for virus-free rapid propagation by utilizing strawberry anther culture, and relates to the technical field of strawberry anther culture, and the method is characterized by comprising the following steps: S1, selection and disinfection of explants, S2, culture stage, S3, ploidy identification, S4, virus detection, and S5, domestication and transplanting of virus-free seedlings. According to the strawberry anther in-vitro culture method, a large number of virus-free plants are successfully obtained through a strawberry anther in-vitro culture technology, the strawberry detoxification efficiency and effect are remarkably improved, and technical support is provided for healthy development of the strawberry industry.

The present invention discloses a method for rapidly obtaining uniform red callus from tea plants using leaves from Anji white tea tissue culture seedlings. Using the leaves of virus-free tissue culture seedlings from Anji white tea as explants, callus was induced using MS medium containing 6-BA and IBA, achieving a high-frequency induction rate of 99.23%. The primary callus was transferred to dark conditions and induced with TDZ and IBA to obtain white callus. The white callus was then transferred to light conditions to obtain uniform red callus. This method reduces the step of explant disinfection, simplifies the operation, and provides a new method for rapidly extracting anthocyanins from tea plants.

The application discloses a method for quickly obtaining garlic virus-free seedlings through in-vitro culture of young scale buds, which comprises the following steps: firstly, taking garlic plants; in the early stage of the expansion of garlic scale buds, peeling off small scale buds and soaking them in sterile water; then sterilizing the small scale buds in a sodium hypochlorite solution; washing the small scale buds with sterile water for multiple times; culturing the small scale buds in a MS medium to make them root and germinate; when the small scale buds have 3-4 leaves, transplanting the small scale buds into a culture pot to harden the seedlings; when the small scale buds have 5-6 leaves, transplanting the garlic seedlings with soil into a field and normally maintaining the garlic seedlings. The method has the advantages of simple operation, reduced requirement for the operation skills of technical personnel, short culture period, quick acquisition of regenerated plants, improved virus-removal efficiency, satisfaction of the market demand for virus-free garlic, production of a large number of virus-free plants in a short time, improved production efficiency, suitability for large-scale production, support for the rapid growth and regeneration of plants, and expectation of becoming an innovative breakthrough in the field of garlic virus-removal culture.

The application discloses a rhabdovirus-free Sf9 cell screening method of double pretreated conditioned medium, relates to the technical field of biology, and obtains the rhabdovirus-free Sf9 cell by double pretreatment of the conditioned medium through cross-flow filtration and beta-propiolactone inactivation and hydrolysis, and combination of single cell cloning screening. The method realizes efficient virus interception and high-activity retention of nutritional factors, the single cell cloning formation rate is greater than 40%, and is suitable for virus-free cell bank construction and biological product production.

The invention discloses a stem tip detoxification method for a specific strain of fresh edible sweet potatoes. According to the method, stem tips of excellent sweet potato silky strains are collected from Wenshan areas of Yunnan province as explants, various conditions of the silky strains in the tissue culture process are explored through sterile treatment of the stem tips, starting of the stem tips, differentiation and growth of buds, rooting culture and selection of plant growth regulators, and a stem tip detoxification tissue culture system of the excellent silky strains is established for the first time; a foundation is laid for providing virus-free high-quality seedlings on a large scale, the problem of variety degeneration is directly solved, and the fresh sweet potato industry upgrading is promoted.

The invention belongs to the technical field of avian influenza vaccines, and particularly relates to an H7N9 subtype avian influenza recombinant strain and an H7N9 subtype avian influenza virus vaccine. The H7N9 subtype avian influenza recombinant virus is formed by an HA gene and an NA gene which are transformed from an H7N9 subtype avian influenza epidemic strain and six internal genes PB2, PB1, PA, NP, NS and M of an H5N2 subtype avian influenza virus D7 strain, and the H7N9 subtype avian influenza recombinant virus is obtained by further using a chicken liver cancer suspension cell system as a carrier for proliferation. The HA titer of the obtained proliferated virus is higher than that of a traditional chick embryo proliferated virus, the virus yield is remarkably increased, after the prepared vaccine is used for immunizing SPF chickens, the HI antibody titer is remarkably higher than that of a chick embryo source vaccine, the challenge protection rate reaches 100%, and no virus is discharged. The technology provides an efficient and reliable solution for prevention and control of avian influenza.

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.

The present invention relates to the technical field of the production of biological products and blood products, and particularly relates to a virus removal filtration system for in-situ sterilization of a nano-membrane and an operation method thereof. The virus removal filtration system includes a nano-membrane, with an A port and a B port respectively provided at both ends of the nano-membrane, and a C port and a D port provided on the side wall of the nano-membrane. It also includes a pre-membrane pipeline, a post-membrane pipeline and a first pipeline arranged in the virus contamination risk area. The feeding end of the pre-membrane pipeline is respectively connected to a buffer tank and a material tank, and the buffer tank is connected to the material tank. The nano-membrane is detachably installed on the pre-membrane pipeline and the post-membrane pipeline. When the nano-membrane is installed, the pre-membrane pipeline is connected to the A port and the B port of the nano-membrane, and the post-membrane pipeline is connected to the C port and the D port of the nano-membrane. The first pipeline is connected to the virus-free area and is connected in parallel with the post-membrane pipeline. The present invention does not involve the transfer of regional articles and completes all operations in the virus contamination risk area.

The invention discloses an oat-medicago sativa continuous cropping planting method which comprises the following steps: step 1, land selection: land to be cultivated is leveled and ploughed, weeds are removed, then a compound fertilizer is applied to the soil, the soil is subjected to rotary tillage after fertilization, and the application amount of the compound fertilizer is 20-25 kg / mu; 2, seed selection and seed treatment: selecting healthy, virus-free, non-invasive and bright oat seeds with the storage time not exceeding one year; healthy, virus-free, noninvasive and bright alfalfa seeds with the storage time not exceeding one year are selected to be subjected to coating or rhizobium seed dressing treatment, the root system of oat grass can improve the soil stability and the moisture retention capacity and improve the soil structure, the soil jacking capacity of oat is high, alfalfa seedling emergence is facilitated, and the seedling survival rate of alfalfa is increased. Meanwhile, the alfalfa and the oat grass are different in growth speed and height, so that competition between the alfalfa and the oat grass can be reduced, and land and sunlight resources are more effectively utilized.

This invention provides a method for preparing a composite solution of sodium hyaluronate and recombinant type III humanized collagen. The invention utilizes artificial screening and design to obtain recombinant type III humanized collagen COL3A1-QS-1, which is highly efficient in secretion and expression in Pichia pastoris. Compared with animal-derived collagen, it has advantages such as uniform molecular weight, high purity, no risk of viral transmission, low rejection reaction, and high bioactivity. Sodium hyaluronate absorbs water and swells into a gel, physically filling tissue volume loss and thus achieving the effect of filling wrinkles and smoothing fine lines. Recombinant type III humanized collagen provides a scaffold and favorable microenvironment for cells, promoting cell adhesion, proliferation, growth, migration, and support for cells and tissues. The composite solution of sodium hyaluronate and recombinant type III humanized collagen prepared from these two components stimulates collagen production by improving the local internal environment, thereby improving superficial wrinkles.

The utility model belongs to the technical field of citrus virus-free seedling rapid grafting fixing devices, and discloses a citrus virus-free seedling rapid grafting fixing device which comprises a supporting plate, a fixing plate is arranged on one side of the supporting plate, a moving groove is formed in one side of the supporting plate, the moving groove is connected with a sliding block in a sliding mode, and the sliding block is connected with the fixing plate in a sliding mode. A clamping plate is connected to the side, away from the moving groove, of the sliding block, and a rotary knob is rotationally connected to one side of the supporting plate. According to the device, through cooperative use of the threaded rod, the sliding block and the rotary knob, the clamping plate can be conveniently driven to move towards the fixing plate to clamp citrus branches, and through arrangement of an acrylic observation plate, the clamping process can be conveniently observed.

The invention discloses a method for removing PVY from potato tissue culture seedlings through composite pretreatment and application. The method comprises the following steps: taking PVY virus infected potato buds for pre-culture; culturing the pre-cultured tissue culture seedlings under the conditions of normal daily temperature and high night temperature; and stripping micro stem tips for culture, and culturing virus-free tissue culture seedlings. Compared with a conventional culture micro-stem tip detoxification method, the technology has the advantage that the detoxification rate is obviously improved. Compared with a whole-course high-temperature micro-stem tip treatment culture method, the stem tip survival rate and the seedling rate are remarkably improved. According to the method, the tissue culture seedling is taken as a core treatment object, and a pretreatment mode of combining chemical reagent combination and variable high temperature is adopted, so that the dependence of a detoxification material on seasons is broken, the detoxification operation can be flexibly and continuously carried out, and the treatment efficiency of a unit space is remarkably improved; the micro-stem tip operation process is synchronously simplified, the technical implementation threshold is reduced, the stem tip activity can be effectively enhanced, the micro-stem tip growth speed is greatly increased, and the practicability and application value of the detoxification technology are comprehensively improved.