8 results about "Virus free" patented technology

The invention relates to the technical field of blueberry tissue culture and virus-free seedling culture, and particularly discloses an efficient culture method for blueberry virus-free tissue culture seedlings, which comprises the following steps: selecting and disinfecting explants: selecting virus-free blueberry stem tips as the explants; performing callus induction; bud differentiation culture: preparing a culture medium by adding ganoderma leukemia polysaccharide and the like into a WPM differential culture medium; rooting culture: preparing a culture medium, namely adding 0.8 mg / L of IBA, 0.2 mg / L of NAA, 20g / L of sucrose, 15mg / L of ganoderma leukemia polysaccharide, 7g / L of agar and the like into a WPM rooting culture medium. By adopting the method disclosed by the invention, the bud differentiation rate reaches 92.7%, no vitrification phenomenon occurs, the ganoderma leukemia polysaccharide regulates cell osmotic pressure and optimizes metabolic balance, the vitrification problem of tissue culture seedlings is solved, the seedlings are more robust, and the generation of malformed seedlings is reduced; the seedling stress resistance is enhanced, and the transplanting survival rate is increased.

The invention discloses a Tongling white ginger stem tip detoxification and propagation method which comprises the following steps: (1) disinfecting the surface of a Tongling white ginger tuber, separating bud points, and inoculating the bud points into an induction culture medium for induction culture of adventitious buds; (2) stripping stem tip meristem from the adventitious buds induced in the step (1), inoculating the stem tip meristem into a regeneration culture medium, and synchronously inducing buds and roots to obtain a complete regeneration plant; wherein the size of the stem tip meristem is 1-2 mm; and (3) selecting the completely detoxified Tongling white ginger regenerated plant obtained in the step (2) as a propagation material, taking the stem node as an explant, and inoculating the Tongling white ginger regenerated plant into a proliferation culture medium for sterile propagation to obtain Tongling white ginger seedlings. According to the method, a special efficient detoxification regeneration technology system for Tongling white ginger is established, virus-free, high-quality and healthy seedlings are provided for industrial cultivation of Tongling white ginger, and accurate protection and inheritance of excellent germplasm resources of national geographical indication products are achieved.

The application provides a method for annual propagation of virus-free sweet potato seedlings by water culture, which comprises the following steps: washing the tissue culture seedlings detected free of virus and then soaking the seedlings in a treatment solution; transplanting the pretreated tissue culture seedlings to a seedling plate in a water tank, pouring the first nutrient solution into the water tank to promote the seedlings to rapidly root, and maintaining a certain temperature, humidity and light in the water culture room; replacing the nutrient solution in the water tank with the second nutrient solution after a certain period of growth, and regularly replacing the second nutrient solution; after the tissue culture seedlings grow into plants with a certain stem node, cutting the stem node segments, soaking the stem node segments in the treatment solution, and then repeating step (2) to obtain cutting seedlings, and spraying the plants with the growth-promoting solution to promote the growth of branches; repeating step (3) to culture the plants with the cut stem segments and the cutting seedlings, and regularly spraying the plants with the foliar nutrient solution and the insecticide. The propagation method is used for the propagation of virus-free seedlings by water culture, and the propagation of the seedlings is simple, efficient and easy to manage, and the annual and efficient propagation of the virus-free sweet potato seedlings can be realized.

The application discloses a rhabdovirus-free Sf9 cell screening method of double pretreated conditioned medium, relates to the technical field of biology, and obtains the rhabdovirus-free Sf9 cell by double pretreatment of the conditioned medium through cross-flow filtration and beta-propiolactone inactivation and hydrolysis, and combination of single cell cloning screening. The method realizes efficient virus interception and high-activity retention of nutritional factors, the single cell cloning formation rate is greater than 40%, and is suitable for virus-free cell bank construction and biological product production.

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.

This invention provides a method for preparing a composite solution of sodium hyaluronate and recombinant type III humanized collagen. The invention utilizes artificial screening and design to obtain recombinant type III humanized collagen COL3A1-QS-1, which is highly efficient in secretion and expression in Pichia pastoris. Compared with animal-derived collagen, it has advantages such as uniform molecular weight, high purity, no risk of viral transmission, low rejection reaction, and high bioactivity. Sodium hyaluronate absorbs water and swells into a gel, physically filling tissue volume loss and thus achieving the effect of filling wrinkles and smoothing fine lines. Recombinant type III humanized collagen provides a scaffold and favorable microenvironment for cells, promoting cell adhesion, proliferation, growth, migration, and support for cells and tissues. The composite solution of sodium hyaluronate and recombinant type III humanized collagen prepared from these two components stimulates collagen production by improving the local internal environment, thereby improving superficial wrinkles.

The utility model belongs to the technical field of citrus virus-free seedling rapid grafting fixing devices, and discloses a citrus virus-free seedling rapid grafting fixing device which comprises a supporting plate, a fixing plate is arranged on one side of the supporting plate, a moving groove is formed in one side of the supporting plate, the moving groove is connected with a sliding block in a sliding mode, and the sliding block is connected with the fixing plate in a sliding mode. A clamping plate is connected to the side, away from the moving groove, of the sliding block, and a rotary knob is rotationally connected to one side of the supporting plate. According to the device, through cooperative use of the threaded rod, the sliding block and the rotary knob, the clamping plate can be conveniently driven to move towards the fixing plate to clamp citrus branches, and through arrangement of an acrylic observation plate, the clamping process can be conveniently observed.

The invention discloses a method for removing PVY from potato tissue culture seedlings through composite pretreatment and application. The method comprises the following steps: taking PVY virus infected potato buds for pre-culture; culturing the pre-cultured tissue culture seedlings under the conditions of normal daily temperature and high night temperature; and stripping micro stem tips for culture, and culturing virus-free tissue culture seedlings. Compared with a conventional culture micro-stem tip detoxification method, the technology has the advantage that the detoxification rate is obviously improved. Compared with a whole-course high-temperature micro-stem tip treatment culture method, the stem tip survival rate and the seedling rate are remarkably improved. According to the method, the tissue culture seedling is taken as a core treatment object, and a pretreatment mode of combining chemical reagent combination and variable high temperature is adopted, so that the dependence of a detoxification material on seasons is broken, the detoxification operation can be flexibly and continuously carried out, and the treatment efficiency of a unit space is remarkably improved; the micro-stem tip operation process is synchronously simplified, the technical implementation threshold is reduced, the stem tip activity can be effectively enhanced, the micro-stem tip growth speed is greatly increased, and the practicability and application value of the detoxification technology are comprehensively improved.