26 results about "Virus free" patented technology

A macrobrachium rosenbergii breeding method belongs to the technical field of shrimp breeding, and comprises the following steps: selection of parent shrimps: selecting a macrobrachium rosenbergii pseudo-female population introduced from Thailand as female shrimps, and stocking in a breeding pond A; screening domestic male groups which are robust in physique and are not carried by viruses, and stocking the male groups in the breeding pond B; thailand pseudo female shrimps and domestic male shrimps are subjected to cross breeding, and the domestic male shrimps (South Taihu No.3) are subjected to cross breeding with the Thailand pseudo female shrimps by virtue of relatively high low temperature resistance, so that the low temperature resistance of hybrid offspring is improved, and the current breeding situation of Thailand Macrobrachium macrobrachium offspring seeds in China is improved.

The invention provides a method for removing capsicum light mottle viruses in capsicum seeds. The method comprises the following steps: (1) carrying out protease hydrolysis treatment on the capsicum seeds by adopting a protease aqueous solution capable of hydrolyzing virus protein shells; and (2) the pepper seeds treated in the step (1) are subjected to light irradiation in a solution containing a photosensitizer to excite the photosensitizer, so that the pepper seeds without the pepper mild mottle viruses are obtained, and the photosensitizer is a visible light excited photosensitizer. Compared with an existing PMMoV virus resisting method, the virus removing method has the advantages that PMMoV viruses of the pepper seeds can be effectively removed, the germination potential and the germination rate of the pepper seeds are not reduced, and the method is more efficient, economical, environmentally friendly and convenient to popularize; the method can promote the prevention and control of pepper virus diseases and the breeding of virus-free pepper seedlings, and guarantee the long-distance transportation of seeds.

The invention relates to the technical field of blueberry tissue culture and virus-free seedling culture, and particularly discloses an efficient culture method for blueberry virus-free tissue culture seedlings, which comprises the following steps: selecting and disinfecting explants: selecting virus-free blueberry stem tips as the explants; performing callus induction; bud differentiation culture: preparing a culture medium by adding ganoderma leukemia polysaccharide and the like into a WPM differential culture medium; rooting culture: preparing a culture medium, namely adding 0.8 mg / L of IBA, 0.2 mg / L of NAA, 20g / L of sucrose, 15mg / L of ganoderma leukemia polysaccharide, 7g / L of agar and the like into a WPM rooting culture medium. By adopting the method disclosed by the invention, the bud differentiation rate reaches 92.7%, no vitrification phenomenon occurs, the ganoderma leukemia polysaccharide regulates cell osmotic pressure and optimizes metabolic balance, the vitrification problem of tissue culture seedlings is solved, the seedlings are more robust, and the generation of malformed seedlings is reduced; the seedling stress resistance is enhanced, and the transplanting survival rate is increased.

The invention is an apparatus having a protective envelope which is designed and constructed to envelope at least one room region, such that the protective envelope is able to separate the at least one room region from an environment immediately surrounding the protective envelope, and use thereof. Methods for operating a production engineering unit under cleanroom conditions and for creating and operating a virus-free and cross-contamination free room region surrounded by a protective envelope for accommodating persons and / or objects subject to quarantine. The invention has a protective envelope with at least two layers separated by a spacer layer, that is flow-permeable.

The invention discloses a Tongling white ginger stem tip detoxification and propagation method which comprises the following steps: (1) disinfecting the surface of a Tongling white ginger tuber, separating bud points, and inoculating the bud points into an induction culture medium for induction culture of adventitious buds; (2) stripping stem tip meristem from the adventitious buds induced in the step (1), inoculating the stem tip meristem into a regeneration culture medium, and synchronously inducing buds and roots to obtain a complete regeneration plant; wherein the size of the stem tip meristem is 1-2 mm; and (3) selecting the completely detoxified Tongling white ginger regenerated plant obtained in the step (2) as a propagation material, taking the stem node as an explant, and inoculating the Tongling white ginger regenerated plant into a proliferation culture medium for sterile propagation to obtain Tongling white ginger seedlings. According to the method, a special efficient detoxification regeneration technology system for Tongling white ginger is established, virus-free, high-quality and healthy seedlings are provided for industrial cultivation of Tongling white ginger, and accurate protection and inheritance of excellent germplasm resources of national geographical indication products are achieved.

The invention relates to the field of bioengineering, in particular to a recombinant collagen which is characterized by comprising one or more of the following characteristics: a, an amino acid sequence of the collagen is shown as any one of SEQ ID NO.1-4; and b. A, the collagen is added, substituted or deleted with one or more amino acids, and the protein has collagen activity. The recombinant collagen prepared by utilizing a genetic engineering technology has the remarkable advantages of stable quality, no virus transmission risk and the like, and has a good application prospect in the field of medical materials.

The invention relates to the technical field of agriculture, in particular to a tomato seed cultivation method. Comprising the following steps: S1, cleaning and disinfecting male and female parent seeds, sowing the disinfected male and female parent seeds, spraying a detoxification agent, and performing illumination culture; s2, stem tips of top growing points are cut and placed on an induction culture medium to be subjected to induction culture, and tomato seedlings are obtained; s3, performing virus detection on the leaves of the tomato seedlings to obtain virus-free male parent and female parent single plants; s4, carrying out propagation expanding; s5, putting the male and female parent seedlings obtained by propagation on a rooting culture medium for culture, and performing acclimatization and transplanting to obtain virus-free male and female parent seedlings; s6, planting the obtained virus-free male and female parent seedlings, and hybridizing to obtain the tomato seeds. The tomato seeds obtained by the cultivation method disclosed by the invention do not contain viruses, and have very high propagation coefficient, survival rate and germination rate.

The application provides a method for annual propagation of virus-free sweet potato seedlings by water culture, which comprises the following steps: washing the tissue culture seedlings detected free of virus and then soaking the seedlings in a treatment solution; transplanting the pretreated tissue culture seedlings to a seedling plate in a water tank, pouring the first nutrient solution into the water tank to promote the seedlings to rapidly root, and maintaining a certain temperature, humidity and light in the water culture room; replacing the nutrient solution in the water tank with the second nutrient solution after a certain period of growth, and regularly replacing the second nutrient solution; after the tissue culture seedlings grow into plants with a certain stem node, cutting the stem node segments, soaking the stem node segments in the treatment solution, and then repeating step (2) to obtain cutting seedlings, and spraying the plants with the growth-promoting solution to promote the growth of branches; repeating step (3) to culture the plants with the cut stem segments and the cutting seedlings, and regularly spraying the plants with the foliar nutrient solution and the insecticide. The propagation method is used for the propagation of virus-free seedlings by water culture, and the propagation of the seedlings is simple, efficient and easy to manage, and the annual and efficient propagation of the virus-free sweet potato seedlings can be realized.

The invention discloses a method for virus-free rapid propagation by utilizing strawberry anther culture, and relates to the technical field of strawberry anther culture, and the method is characterized by comprising the following steps: S1, selection and disinfection of explants, S2, culture stage, S3, ploidy identification, S4, virus detection, and S5, domestication and transplanting of virus-free seedlings. According to the strawberry anther in-vitro culture method, a large number of virus-free plants are successfully obtained through a strawberry anther in-vitro culture technology, the strawberry detoxification efficiency and effect are remarkably improved, and technical support is provided for healthy development of the strawberry industry.

The invention discloses a method for rapidly obtaining virus-free garlic seedlings by in-vitro culture of young and tender bulbil, which comprises the following steps: firstly, taking a garlic plant, stripping small bulbil to soak in sterile water at the initial expansion stage of the garlic bulbil, then sterilizing in a sodium hypochlorite solution, washing with sterile water for multiple times, culturing the small bulbil by using an MS culture medium to root and germinate, and after the 3-4 leaf stage, culturing the small bulbil to obtain the virus-free garlic seedlings. Transplanting the garlic seedlings into a culture bowl for seedling hardening, transplanting the obtained garlic seedlings with soil into a field after the garlic seedlings grow to a 5-6-leaf period, and performing normal maintenance. The method is easy to operate, and the requirement for the operation skill of technicians is reduced; the culture period is short, and regenerated plants can be rapidly obtained, so that the detoxification efficiency is improved, and the demand of the market on virus-free garlic is met; a large number of virus-free plants can be generated in a short time, the production efficiency is improved, and the method is suitable for large-scale production; rapid growth and regeneration of plants can be supported, and the method is expected to become an innovative breakthrough in the field of garlic detoxification culture.

The present invention discloses a method for rapidly obtaining uniform red callus from tea plants using leaves from Anji white tea tissue culture seedlings. Using the leaves of virus-free tissue culture seedlings from Anji white tea as explants, callus was induced using MS medium containing 6-BA and IBA, achieving a high-frequency induction rate of 99.23%. The primary callus was transferred to dark conditions and induced with TDZ and IBA to obtain white callus. The white callus was then transferred to light conditions to obtain uniform red callus. This method reduces the step of explant disinfection, simplifies the operation, and provides a new method for rapidly extracting anthocyanins from tea plants.

The application discloses a method for quickly obtaining garlic virus-free seedlings through in-vitro culture of young scale buds, which comprises the following steps: firstly, taking garlic plants; in the early stage of the expansion of garlic scale buds, peeling off small scale buds and soaking them in sterile water; then sterilizing the small scale buds in a sodium hypochlorite solution; washing the small scale buds with sterile water for multiple times; culturing the small scale buds in a MS medium to make them root and germinate; when the small scale buds have 3-4 leaves, transplanting the small scale buds into a culture pot to harden the seedlings; when the small scale buds have 5-6 leaves, transplanting the garlic seedlings with soil into a field and normally maintaining the garlic seedlings. The method has the advantages of simple operation, reduced requirement for the operation skills of technical personnel, short culture period, quick acquisition of regenerated plants, improved virus-removal efficiency, satisfaction of the market demand for virus-free garlic, production of a large number of virus-free plants in a short time, improved production efficiency, suitability for large-scale production, support for the rapid growth and regeneration of plants, and expectation of becoming an innovative breakthrough in the field of garlic virus-removal culture.

The application discloses a rhabdovirus-free Sf9 cell screening method of double pretreated conditioned medium, relates to the technical field of biology, and obtains the rhabdovirus-free Sf9 cell by double pretreatment of the conditioned medium through cross-flow filtration and beta-propiolactone inactivation and hydrolysis, and combination of single cell cloning screening. The method realizes efficient virus interception and high-activity retention of nutritional factors, the single cell cloning formation rate is greater than 40%, and is suitable for virus-free cell bank construction and biological product production.

The invention discloses a stem tip detoxification method for a specific strain of fresh edible sweet potatoes. According to the method, stem tips of excellent sweet potato silky strains are collected from Wenshan areas of Yunnan province as explants, various conditions of the silky strains in the tissue culture process are explored through sterile treatment of the stem tips, starting of the stem tips, differentiation and growth of buds, rooting culture and selection of plant growth regulators, and a stem tip detoxification tissue culture system of the excellent silky strains is established for the first time; a foundation is laid for providing virus-free high-quality seedlings on a large scale, the problem of variety degeneration is directly solved, and the fresh sweet potato industry upgrading is promoted.

The invention relates to the technical field of grouper breeding, and discloses a grouper factory-like artificial breeding method which comprises the following steps: a, preparing a nursery pond: selecting a square cement pond with the specification of 6m * 6m * 1.3 m and the water depth of 1m, arranging a running water inflation system, controlling the water quality to be natural seawater, controlling the water temperature to be 25-28 DEG C, controlling the salinity to be 27-29 and controlling the illumination intensity to be 6000-8000 lux; b, roe distribution: distributing healthy and virus-free grouper roes according to the density of 150g in each pond, wherein the roe distribution density is 4g / m < 3 >; healthy and virus-free roes are strictly selected for incubation, and accurate water quality control and illumination management are combined, so that the incubation success rate of the roes is greatly improved. In the early hatching stage of the fry, the water quality is strictly controlled, it is ensured that the fry can develop in the most suitable environment, and the death rate caused by the water quality problem in the hatching process is effectively reduced. Therefore, the survival rate of the fish fries is remarkably improved, and the stability of subsequent breeding is ensured.

The invention belongs to the technical field of avian influenza vaccines, and particularly relates to an H7N9 subtype avian influenza recombinant strain and an H7N9 subtype avian influenza virus vaccine. The H7N9 subtype avian influenza recombinant virus is formed by an HA gene and an NA gene which are transformed from an H7N9 subtype avian influenza epidemic strain and six internal genes PB2, PB1, PA, NP, NS and M of an H5N2 subtype avian influenza virus D7 strain, and the H7N9 subtype avian influenza recombinant virus is obtained by further using a chicken liver cancer suspension cell system as a carrier for proliferation. The HA titer of the obtained proliferated virus is higher than that of a traditional chick embryo proliferated virus, the virus yield is remarkably increased, after the prepared vaccine is used for immunizing SPF chickens, the HI antibody titer is remarkably higher than that of a chick embryo source vaccine, the challenge protection rate reaches 100%, and no virus is discharged. The technology provides an efficient and reliable solution for prevention and control of avian influenza.

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.

The present invention provides a method for removing pepper mild mottle virus from pepper seeds, which comprises: (1) performing protease hydrolysis treatment on pepper seeds with an aqueous protease solution capable of hydrolyzing the viral protein coat; (2) irradiating the pepper seeds treated in step (1) with light in a solution containing a photosensitizer to excite the photosensitizer, thereby obtaining pepper seeds from which the pepper mild mottle virus has been removed, wherein the photosensitizer is a photosensitizer excited by visible light. The virus removal method provided by the present invention can not only effectively remove the PMMoV virus from pepper seeds compared with the existing methods for combating PMMoV virus disease, but also does not reduce the germination potential and germination rate of pepper seeds, and is more efficient, economical, environmentally friendly and easy to promote; it can promote the prevention and control of pepper virus disease and the breeding of virus-free pepper seedlings, and escort the long-distance transportation of seeds.

The present invention relates to the technical field of the production of biological products and blood products, and particularly relates to a virus removal filtration system for in-situ sterilization of a nano-membrane and an operation method thereof. The virus removal filtration system includes a nano-membrane, with an A port and a B port respectively provided at both ends of the nano-membrane, and a C port and a D port provided on the side wall of the nano-membrane. It also includes a pre-membrane pipeline, a post-membrane pipeline and a first pipeline arranged in the virus contamination risk area. The feeding end of the pre-membrane pipeline is respectively connected to a buffer tank and a material tank, and the buffer tank is connected to the material tank. The nano-membrane is detachably installed on the pre-membrane pipeline and the post-membrane pipeline. When the nano-membrane is installed, the pre-membrane pipeline is connected to the A port and the B port of the nano-membrane, and the post-membrane pipeline is connected to the C port and the D port of the nano-membrane. The first pipeline is connected to the virus-free area and is connected in parallel with the post-membrane pipeline. The present invention does not involve the transfer of regional articles and completes all operations in the virus contamination risk area.

The invention relates to the technical field of fruit tree germplasm resource preservation and breeding, in particular to a method capable of rapidly breeding strawberry virus-free original seedlings all year round. According to the method provided by the invention, a sterilized plastic box (containing vermiculite) with air holes and a rooting nutrient solution are adopted, and the adaptability of transplanted seedlings to the environment is greatly improved through virus-free original seedling rooting induction, simple photoacclimatization and soil-carrying transplanting, so that the seasonal limitation in the test-tube plantlet transplanting process is eliminated, the transplanting time is shortened, and the survival rate of the test-tube plantlets is increased. And the transplanting survival rate is improved (more than 95%). According to the method provided by the invention, the breeding process of the secondary original seedlings is shortened and simplified as much as possible by improving the seedling cultivation technical means, the survival rate of the seedlings is improved, the method is not limited to specific seasons, virus-free original seedlings can be provided for strawberry production all the year round, and the method is suitable for large-scale production. The problems of high cost, complex links, mixed varieties, high variation probability and the like in the tissue culture process are greatly avoided.

The invention discloses a stem tip detoxification tissue culture seedling method for octaploid strawberry red. The method comprises the following steps: selecting an explant; stem tip treatment; performing primary culture; performing blank culture; carrying out subculture; performing rooting culture; transplanting and domesticating seedlings and planting. Through explant selection, application opportunity selection of a blank culture medium and subculture composite hormone proportion design, original seedlings of the virus-free seedlings of the Hongyan strawberries are successfully cultivated, the virus-free efficiency reaches 98% or above, and the transplanting survival rate reaches 99% or above. According to the method, the seedling culture time can be greatly saved, the blank that no stem tip tissue rapid propagation system exists in the Hongyan strawberries at present is filled up, higher detoxification efficiency and survival rate are achieved, and a foundation is laid for virus-free rapid culture of the Hongyan strawberries.

The invention discloses an oat-medicago sativa continuous cropping planting method which comprises the following steps: step 1, land selection: land to be cultivated is leveled and ploughed, weeds are removed, then a compound fertilizer is applied to the soil, the soil is subjected to rotary tillage after fertilization, and the application amount of the compound fertilizer is 20-25 kg / mu; 2, seed selection and seed treatment: selecting healthy, virus-free, non-invasive and bright oat seeds with the storage time not exceeding one year; healthy, virus-free, noninvasive and bright alfalfa seeds with the storage time not exceeding one year are selected to be subjected to coating or rhizobium seed dressing treatment, the root system of oat grass can improve the soil stability and the moisture retention capacity and improve the soil structure, the soil jacking capacity of oat is high, alfalfa seedling emergence is facilitated, and the seedling survival rate of alfalfa is increased. Meanwhile, the alfalfa and the oat grass are different in growth speed and height, so that competition between the alfalfa and the oat grass can be reduced, and land and sunlight resources are more effectively utilized.

The invention discloses a preparation method of an organic fermented fertilizer based on livestock and poultry animal manure, which comprises the following steps: selecting fresh and virus-free livestock and poultry animal manure as a compost raw material, inoculating a leavening agent, and adding composite traditional Chinese medicine residue, biomass charcoal and modified bentonite for primary fermentation; humic acid and ferrous sulfate are added in the primary turning process, and secondary fermentation is performed after uniform mixing; the composite traditional Chinese medicine residues comprise folium artemisiae argyi, rhizoma atractylodis, folium isatidis and garlic straw; based on the dry mass of the compost raw material, the addition amount of the composite traditional Chinese medicine residue is 10-15 wt.%, the addition amount of the biomass charcoal is 2-3.5 wt.%, the addition amount of the modified bentonite is 5-7 wt.%, the addition amount of the humic acid is 5-8 wt.%, and the addition amount of the ferrous sulfate is 0.5-1 wt.%. The livestock and poultry manure is used as a raw material, and through material blending and improvement, volatilization of malodorous substances such as ammonia gas in the fermentation process is reduced through adsorption and conversion, and environmental pollution is reduced. The nitrogen loss can be avoided, the compost is thoroughly decomposed, and the rich nutritional ingredients in the compost are ensured.

This invention provides a method for preparing a composite solution of sodium hyaluronate and recombinant type III humanized collagen. The invention utilizes artificial screening and design to obtain recombinant type III humanized collagen COL3A1-QS-1, which is highly efficient in secretion and expression in Pichia pastoris. Compared with animal-derived collagen, it has advantages such as uniform molecular weight, high purity, no risk of viral transmission, low rejection reaction, and high bioactivity. Sodium hyaluronate absorbs water and swells into a gel, physically filling tissue volume loss and thus achieving the effect of filling wrinkles and smoothing fine lines. Recombinant type III humanized collagen provides a scaffold and favorable microenvironment for cells, promoting cell adhesion, proliferation, growth, migration, and support for cells and tissues. The composite solution of sodium hyaluronate and recombinant type III humanized collagen prepared from these two components stimulates collagen production by improving the local internal environment, thereby improving superficial wrinkles.

The utility model belongs to the technical field of citrus virus-free seedling rapid grafting fixing devices, and discloses a citrus virus-free seedling rapid grafting fixing device which comprises a supporting plate, a fixing plate is arranged on one side of the supporting plate, a moving groove is formed in one side of the supporting plate, the moving groove is connected with a sliding block in a sliding mode, and the sliding block is connected with the fixing plate in a sliding mode. A clamping plate is connected to the side, away from the moving groove, of the sliding block, and a rotary knob is rotationally connected to one side of the supporting plate. According to the device, through cooperative use of the threaded rod, the sliding block and the rotary knob, the clamping plate can be conveniently driven to move towards the fixing plate to clamp citrus branches, and through arrangement of an acrylic observation plate, the clamping process can be conveniently observed.

The invention discloses a method for removing PVY from potato tissue culture seedlings through composite pretreatment and application. The method comprises the following steps: taking PVY virus infected potato buds for pre-culture; culturing the pre-cultured tissue culture seedlings under the conditions of normal daily temperature and high night temperature; and stripping micro stem tips for culture, and culturing virus-free tissue culture seedlings. Compared with a conventional culture micro-stem tip detoxification method, the technology has the advantage that the detoxification rate is obviously improved. Compared with a whole-course high-temperature micro-stem tip treatment culture method, the stem tip survival rate and the seedling rate are remarkably improved. According to the method, the tissue culture seedling is taken as a core treatment object, and a pretreatment mode of combining chemical reagent combination and variable high temperature is adopted, so that the dependence of a detoxification material on seasons is broken, the detoxification operation can be flexibly and continuously carried out, and the treatment efficiency of a unit space is remarkably improved; the micro-stem tip operation process is synchronously simplified, the technical implementation threshold is reduced, the stem tip activity can be effectively enhanced, the micro-stem tip growth speed is greatly increased, and the practicability and application value of the detoxification technology are comprehensively improved.