The invention discloses an
analysis method for simultaneously measuring residues of 12 sulfonamide medicaments, 19
quinolone medicaments and 8
benzimidazole medicaments and metabolites thereof in
chicken liver by quick, easy, cheap, effective, rugged and safe (
QuEChERS) extraction and purification-ultrahigh performance liquid
chromatography-
tandem mass spectrometry (
QuEChERS-UPLC-MS / MS). The method comprises the following steps of: extracting a sample by using 1 percent
acetic acid-
acetonitrile solution, purifying the sample by using NH2 adsorbent, and
degreasing the sample by using n-
hexane;and then performing separation by using a Kromasil Eternity C18
chromatographic column (100mm*2.1mm, 2.5mum), performing
gradient elution by using 0.1 percent
formic acid and
methanol as mobile phases, performing
ionization in an
electron spray positive
ion (ESI<+>) mode, performing detection in a multi-reaction monitoring (MRM) mode, and performing quantification by an
internal standard method. The 39 medicaments have good
linearity (r is more than 0.98) in a blank adding concentration range of 5 to 100mug / kg, the average
recovery rate of the medicaments in the adding level range of 10 to 50mug / kg, the
relative standard deviation (RSD) is 1.5 to 23.4 percent, the limit of detection (LOD) of the 39 medicaments is 5mug / kg, and the low limit of quantification (LOQ) is 10mug / kg. The method is simple, convenient, quick, sensitive, accurate and rugged, and is suitable for confirmation and
quantitative determination of the residues of the residues of the sulfonamide,
quinolone and
benzimidazole medicaments in the
chicken liver.