37 results about "Chicken Liver" patented technology

This invention relates to the field of pet nutrition technology, and more particularly to a fresh dog food for improving coat health and its preparation method. The fresh dog food of this invention comprises component A and component B. Component A includes the following components in parts by weight: 40-45 parts venison, 10-12 parts mussels, 8-10 parts chicken liver, 8-10 parts duck egg, 3-5 parts carrot, 3-5 parts broccoli, 3-5 parts pumpkin, 3-5 parts taro, 3-5 parts avocado, 0.8-1 part dried red dates, 0.8-1 part dried rose petals, 0.5 parts whey calcium, 1.3 parts malt powder, 0.5 parts inulin, 0.5 parts brewer's yeast, and 0.2 parts fish oil. Component B is Bacillus subtilis; the viable count of Bacillus subtilis is 1×10⁻⁶. 9 CFU / g. This invention utilizes the synergistic effect of deep-sea shellfish and venison to improve the coat quality of puppies, increasing the coat diameter by ≥23.1% and the scale thickness by ≥32.4%.

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The present application provides a kind of complex probiotic paste, which combines Enterococcus faecalis, Saccharomyces cerevisiae and vitamin B 12 Producing lactic acid bacteria of canine source, adds prebiotics, flavoring substances and auxiliary substances such as paste matrix preparation.In the paste, the viable count of Enterococcus faecalis, Saccharomyces cerevisiae and lactic acid bacteria is 1-2:1-2:1-8;Paste matrix includes fructooligosaccharide 0.5-3%, psyllium husk powder 0.1-2%, goat milk powder 2-8%, chicken liver powder 1-5%, potassium sorbate 0.5-2%, xanthan gum 1-4%, and the balance is water.The above-mentioned complex probiotic paste can be stably stored for 56 days at 4 DEG C and normal temperature dry place, has good storage stability, good safety, and can adjust intestinal flora and increase the content of intestinal lactic acid bacteria.The above-mentioned complex probiotic paste has good palatability and oral tolerance for pets such as dogs and cats, and has good therapeutic effect on DSS-induced chronic enteritis diarrhea and vitamin B 12 Deficiency.

This invention discloses a coat-enhancing composition for improving the quality of cat fur and its application, belonging to the field of pet food technology. The composition uses a high proportion of fresh chicken meat and fresh bone-in chicken thigh meat as the main protein source, combined with krill oil, fresh chicken liver, various natural plant powders, and compound nutrients, formulated in a scientific ratio. Animal experiments have verified that this invention can significantly increase the amino acid content of cat fur, improve fur luster and saturation, enhance tensile strength, and optimize hair cuticle structure, without adversely affecting the cat's weight, blood routine, blood biochemistry, or other health indicators, demonstrating safety and high efficacy. This invention can be widely applied to the preparation of functional fresh meat baked food for improving the quality of cat fur.

The invention discloses a pet hair-removing composition containing jerusalem artichoke synanthrin and application of the pet hair-removing composition. Comprising 2-6 parts of jerusalem artichoke synanthrin, 5-10 parts of rhizoma sparganii, 10-20 parts of medicinal jujube, 10-20 parts of medicinal pawpaw and 10-20 parts of euonymus bungeanus. Uniformly mixing chicken oil, olive oil and distilled water; stirring while heating, and homogenizing and emulsifying to obtain a first mixed solution; the rhizoma sparganii, the medicinal jujubes, the medicinal papayas and the euonymus bungeanus are crushed and sieved, mixed powder is obtained, jerusalem artichoke synanthrin, the mixed powder, sodium tripolyphosphate, chicken powder, chicken liver powder, sweet potato starch and the remaining distilled water are added into the first mixed solution, the mixture is stirred to be uniform, and homogenizing emulsification is conducted to obtain the pet hair melting paste. The jerusalem artichoke synanthrin is combined with the traditional Chinese medicines rhizoma sparganii, medicinal jujube, medicinal papaya and euonymus bungeanus for use, so that the intestinal tract movement of pets can be accelerated, the problems of diarrhea, constipation and the like cannot be caused, pet hair is promoted to be discharged out of a body, and the components are safe and can be used for a long time.

1. Name of the product in this design: Packaging Bag (Hot Little Chicken Liver). 2. Purpose of this design: This product is used for packaging food. 3. The key design features of this product are its pattern and the combination of pattern and color. 4. The image or photograph that best illustrates the design's key features: the front view. 5. The design for which protection is sought includes color. 6. This design is for a thin product, therefore the left view, right view, top view, and bottom view are omitted. 7. Other situations requiring explanation: Other explanation: The area covered by the gray block in the view is a transparent material.

FIELD: veterinary medicine and dietetics.SUBSTANCE: development of complete feed compositions that can provide a therapeutic effect in cases of exocrine pancreatic insufficiency. The composition of a complete dietary feed for cats with exocrine pancreatic insufficiency has been developed in the following ratio, wt.%: grain part: mixture of rice, wheat, hulled barley and corn – 37.0; protein part of plant origin: isolated soy protein, beet pulp, wheat bran, flax seeds – 22.0; protein part of animal origin: feather meal, chicken liver, egg powder – 25.0; mineral supplement: feed chalk, monocalcium phosphate, table salt, plantain seeds Plantago psyllium, mannanoligasaccharides with beta-glucans and lutein – 2.0; vitamin premix: A, D3, E, K, B1, B2, B3, B5, B6, B12, Bc, H, taurine, inulin, beech lignocellulose, complex of natural fulvic and humic acids – 2.5; flavor enhancer in the form of enzymatic hydrolysate of pork and chicken by-products – 3–3.5; brewer's yeast – 2.5–3.0; a mixture of fish and chicken fat – 5.0–6.0.EFFECT: obtaining food for cats with exocrine pancreatic insufficiency, which is easily reproducible, clinically effective, and convenient in dosing and use.1 cl, 3 tbl, 1 ex

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The invention discloses a pet hair beautifying food and a preparation method thereof. The pet hair beautifying food is prepared from the following raw materials in percentage by weight: 85.0%-88.0% of fresh chicken breast, 5.0%-6.0% of fresh chicken liver, 2.0%-3.0% of krill oil, 1.5%-2.5% of krill meal, 1.0%-2.0% of fish oil and an additive, the additive comprises 0.2% of sodium hyaluronate, 0.1% of natural vitamin E and 0.1% of vitamin B. The pet hair beautifying food is good in palatability and has a hair beautifying effect.

The invention relates to the technical field of nutritional supplement preparations for pets, in particular to a high-activity non-denatured II-type collagen pet joint dispersible tablet and a preparation method thereof. According to the technical scheme, the soft capsule is prepared from the following raw materials in percentage by weight: 4.0%-7.0% of non-modified II type collagen, 4.0%-6.0% of sodium chondroitin sulfate, 4.0%-6.0% of green lip mussel powder, 0.5%-1.5% of sodium hyaluronate, 30%-40% of microcrystalline cellulose, 12%-18% of sodium carboxymethyl starch, 15%-22% of sweet potato starch, 4.0%-6.0% of polyvinylpolypyrrolidone, 6.0%-10.0% of enzymolysis chicken liver powder, 2.0%-4.0% of yeast extract, 1.5%-3.5% of lactose goat milk powder and 0.3%-0.7% of magnesium stearate. A core active component structure is protected by virtue of nano embedding and a whole-course low-temperature process, and a dual joint maintenance path of immunoregulation and nutrition supplement is constructed.

This invention discloses a method and application for regulating fatty liver syndrome based on osteocalcin-adiponectin receptor, relating to the field of livestock and poultry metabolic disease prevention and control technology. The method includes the following steps: clarifying the clinical symptoms and pathological changes of fatty liver syndrome in laying hens; constructing a complementary validation system for fatty liver syndrome models, including an in vivo FLS model in laying hens and an in vitro LMH model of chicken liver cancer cells; using osteocalcin OCN and the adiponectin receptor agonist AdipoRon to target and intervene in the in vivo FLS model and the in vitro LMH model of chicken liver cancer cells, and conducting multi-dimensional detection and evaluation of the regulatory effect; by specifically silencing the expression of AdipoR1 or AdipoR2, comparing changes in lipid deposition and signaling pathways in cells treated with osteocalcin, and identifying the functional hierarchy of AdipoR1 and AdipoR2 in the osteocalcin regulatory pathway; this invention achieves precise prevention and treatment of fatty liver syndrome in laying hens through complementary validation models, targeted intervention with osteocalcin and AdipoRon, and identification of adiponectin receptor function.

A meat core is formed by baking young pigeon meat and chicken, functional components such as deep sea fish oil, linseed oil and the like are embedded in a wall material containing cranberry powder, chicory roots, lactoferrin and zinc to form a functional coating to wrap the meat core, and a vacuum freeze drying technology is combined, so that the activity of the functional components is effectively protected, and the release performance is improved; zinc ions are supplemented, so that the antibacterial property and the stability are enhanced, and the hair health is promoted. The outermost layer of the cat food adopts the chicken liver powder, the valerian extract and chitosan to form a barrier oil film, oxidation is remarkably delayed, the shelf life is prolonged, meanwhile, the chicken liver powder and the valerian extract are used for improving the food calling effect and the emotion relieving effect, and the palatability is further improved. Unification of functional activity protection, texture optimization and product stability is realized.

The present invention discloses a functional puffed cat food for promoting intestinal tract hair discharge and a production method thereof, and relates to the technical field of pet food, and the functional puffed cat food comprises the following functional raw materials: 2% of a cactus extract, 1.5% of semen plantaginis husk powder, 1.5% of pumpkin powder, and the balance of water. The feed also comprises the following basic raw materials: 38% of fresh chicken, 22% of dehydrated chicken, 12% of pigeon meat, 3% of fresh chicken liver, 12% of Peru steam fish meal, enzymolysis chicken powder, cassava starch, yeast hydrolysate, dried apples, turmeric and schizochytrium limacinum powder. The invention successfully provides an innovative cat food product which has a remarkable hair removal function, comprehensive nutrition, excellent palatability, high safety and industrial production feasibility, and a production technology of the innovative cat food product, and effectively solves the technical problem of lack of efficient, safe and convenient hair removal functional food which is liked by pets to eat in the prior art. Good social and economic values are realized.

The invention provides 3A fatty acid pet food capable of improving immunity of young dogs and cats and a preparation method of the 3A fatty acid pet food, and belongs to the technical field of pet food. Firstly, the 3A fatty acid pet food capable of improving immunity of young dogs and cats is provided, the 3A fatty acid pet food comprises the following components: chicken meal, sweet potato flour, potato flour, chicken oil, chicken livers, mineral substances, multivitamins, cellulose, linseed oil, ARA oil and DHA algal oil, the content of ALA in the 3A fatty acid pet food is 0.15-0.60%, the content of ARA is 0.15-0.60%, the content of DHA is 0.15-0.60%, it is guaranteed that the 3A fatty acid pet food contains three kinds of fatty acids of ALA, ARA and DHA, and meanwhile, the content of DHA in the 3A fatty acid pet food can be improved. The contents of three fatty acids, namely ALA, ARA and DHA, are balanced, so that the immunity of the pets is improved; and secondly, the invention further provides a preparation method of the adaptive 3A fatty acid pet food, so that the stable quality of the prepared pet food is ensured, and batch and large-scale production can be realized.

The present application relates to the technical field of microbial inoculant, and particularly relates to a compound microbial inoculant and application of the compound microbial inoculant in preparation of chicken liver flavor seasoning, wherein the compound microbial inoculant is prepared by mixing Lactobacillus plantarum YR07 Lactiplantibacillus plantarum ) and Saccharomyces cerevisiae HQSAJ-001-24 Saccharomyces cerevisiae ) in a volume ratio of 1:1-3, wherein the Lactobacillus plantarum YR07 7 and the Saccharomyces cerevisiae HQSAJ-001-24 are respectively activated and cultured to a viable bacterial count of greater than or equal to 1x10 7 CFU / mL. Through the synergistic effect of the compound microbial inoculant fermentation and Maillard reaction, the inherent fishy smell of chicken liver is completely covered and transformed, bitter peptides are deeply degraded, umami and sweet amino acids are enriched, a compound system containing multiple key flavor substances is constructed, and finally a high-quality natural seasoning with rich flavor levels, fresh and mellow taste and significant flavor enhancement function is prepared.

The invention discloses a bacterial infection and LPS induced broiler chicken liver injury model and a construction method and application thereof, and the construction method comprises the following steps: administering 10-day-old broiler chicken with Escherichia coli E.coli O78 with a preset concentration in an intragastric administration manner; after broiler chickens are subjected to intragastric administration of escherichia coli for 18 hours, an LPS solution is injected into the intraperitoneal cavity of the broiler chickens, after the LPS solution is injected for 24 hours, the broiler chicken liver injury model is obtained, when the broiler chickens are 11 days old, the broiler chickens subjected to intragastric administration of escherichia coli are subjected to intraperitoneal injection of the LPS solution, and after the LPS solution is injected for 24 hours, the broiler chicken liver injury model is obtained. The broiler chicken liver injury model is constructed by intragastric administration of avian pathogenic escherichia coli (E.coli O78) and intraperitoneal injection of LPS (2 mg / kg), the pathology of the model is highly consistent with that of clinical broiler chicken bacterial liver injury, the liver has clinical typical lesions macroscopically, liver cells are degenerative and necrotic microscopically, and a molecular pathway activation mode is consistent with that of clinical application; the model constructed by the invention realizes specific liver injury, only the liver has specific injury, multiple organ lesions such as heart, peritoneum and the like do not exist, and the model availability is high.

The invention belongs to the technical field of animal foods, and relates to a nutritional food for improving joint health of pets and a preparation method thereof. The nutritional food comprises the following raw materials: sweet potato powder, chicken powder, enzymolysis chicken, chicken oil, grains, beef powder, starch, fruit and vegetable powder, chicken liver powder, whole goat milk powder, fish oil, linseed oil, yolk powder, vitamin E, sodium hyaluronate, bovine bone collagen peptide, glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone, a vitamin mineral premix and a Chinese herbal medicine premix. The nutritional food for improving joint health of pets provided by the invention is rich in nutrition, good in palatability and simple in production process, does not lose nutrition of the raw materials, and promotes and improves digestibility of the nutritional food. Animal experiments show that the nutritional food for improving the joint health of the pets has the advantages that the Lequesne score of canine arthritis is reduced, the levels of IL-1beta and TNF-alpha in canine serum are reduced, the level of CTXII in the canine serum is reduced, and the canine osteoarthritis is effectively prevented and treated.

The invention relates to the technical field of food processing, and discloses a dry-fried chicken accessory and a preparation process thereof, and the preparation process comprises the following steps: raw material pretreatment, tenderization treatment, seasoning and pickling, pre-dehydration treatment, grease pretreatment, dry frying process, sterilization and packaging. According to the method disclosed by the invention, aiming at the differences of various accessory parts of the chicken heart, the chicken gizzards, the chicken liver and the chicken intestines in tissue structures and textures, the tenderization liquid containing the specific compound enzyme preparation is used for carrying out synergistic treatment, and protease from different sources acts on different protein components to degrade connective tissues and myofibrils; the activity and action sites of protease are further adjusted through participation of calcium ions and organic acid, so that tenacious chicken gizzards and chicken hearts and relatively tender chicken livers and chicken intestines can synchronously achieve ideal tenderization effects, the taste uniformity and overall tenderness of the final product are improved, and the problem that part of raw materials are dry, hard, soft and rotten is avoided.

The invention relates to the technical field of fish phagostimulants, and discloses an emulsified milk-fishy phagostimulant and a preparation method thereof. Comprising the following raw materials: fresh chicken liver, fructose, sodium bicarbonate, table salt, chicken oil, ethyl maltol, vanillin, capric acid, dodecanoic acid, myristic acid, gamma-nonanolactone, delta-decalactone, gamma-octyl lactone, delta-dodecalactone, milk lactone, 3-hydroxy-2-butanone, an emulsifier and a cosolvent. Chicken livers and milk-flavor components are gradually added through a Maillard reaction, and the crucian carp phagostimulant with natural viscera fragrance and milk flavor is prepared. Compared with a control group, the feed attractant has the advantages that the feed attractant rate is increased by 79%, the feed attractant rate is increased by 15% compared with a single chicken liver Maillard reaction group, the feed coefficient is low, and the advanced level of white crucian carp culture is achieved. Great popularization and utilization values are realized.

The invention discloses a nutritional supplement for pregnant pets and a preparation method of the nutritional supplement. According to the preparation method, after chickpeas are hydrolyzed by protease, GAD enzyme and calcium chloride are added, glutamic acid is induced to be converted into GABA through calcium ions, and meanwhile free amino acid reacts with the calcium ions to generate amino acid chelated calcium; the egg yolk powder and the lactoferrin are utilized to construct a double-emulsion system, so that the GABA is protected from being damaged in advance, the amino acid chelated calcium is slowly released, and the absorption antagonism of mineral substances such as calcium, iron and zinc is reduced. The chicken breast, the chicken liver, the traditional Chinese medicines and other raw materials are chopped, mixed and sterilized to obtain a finished product. The nutritional powder is rich in GABA, soybean isoflavone, Omega-3 fatty acid, vitamin B6, lactoferrin, folic acid and various minerals, can directly or indirectly support progestational hormone secretion and relieve anxiety during pregnancy, meets the requirements of maternal blood replenishing and immunity improving and fetal development nutrition supply, and is suitable for nutrition supplement of pets during pregnancy.

The invention particularly relates to a composite functional pet phagostimulant and a preparation method thereof.The preparation method comprises the following steps that 1, after chicken liver pulp, chicken oil and concentrated protein liquid are subjected to enzymolysis, zymolyte is collected; (2) carrying out Maillard reaction on the zymolyte obtained in the step (1), yeast hydrolysate and reducing sugar step by step; (3) after cooling in the step (2), adding a nutrition enhancer, an intestinal health agent, an antioxidant and a stabilizer, and homogenizing and stabilizing; and (4) adding an acidity regulator and a preservative into the mixture obtained in the step (3), uniformly mixing, filling, sterilizing and packaging. The raw material of the added concentrated protein liquid is cooking residual water of meat, comprehensive utilization can reduce the pressure of wastewater treatment, meanwhile, more nutrient substances are added to the phagostimulant, functional additives in the phagostimulant are stably embedded, the antioxidant slow-release effect and the intestinal tract targeted release effect of prebiotics are enhanced, and the nutritional value of the phagostimulant is improved. The added value of the phagostimulant is improved, and the phagostimulant has a good commercial application prospect.

The application relates to the technical field of biological medicine, and particularly provides a truncated mutant of chicken hepatocyte growth factor and application thereof in preventing and treating liver-related diseases. A nucleic acid molecule constructed based on the truncated mutant of the chicken hepatocyte growth factor and taking E. coli as an expression vector not only realizes efficient expression of the truncated mutant of the chicken hepatocyte growth factor, lays a foundation for industrial application of the truncated mutant of the chicken hepatocyte growth factor, but also has significant biological activity.

The invention discloses a cat phagostimulant, a preparation method thereof and pet food containing the cat phagostimulant. The cat phagostimulant comprises enzymolysis tallow, chicken liver protein enzymatic hydrolysate, xylose and a product of Maillard reaction of cysteine. The lipolysis degree of the enzymolysis tallow is 50-58%; the content of the enzymolysis tallow is 0.5-2.5% of the mass of the chicken liver protein enzymatic hydrolysate; the content of the xylose is 5-7% of the mass of the chicken liver protein enzymatic hydrolysate; the mass of the cysteine is 1%-2% of that of the chicken liver protein enzymatic hydrolysate. According to the cat phagostimulant, the content of characteristic flavor precursor substances is increased through precise enzymolysis of tallow, the chicken liver protein controllable Maillard reaction is cooperated, and a thermal reaction product with multi-layer meat flavor fragrance is directionally constructed, so that the cat phagostimulant with three-dimensional beef flavor and full and harmonious meat fragrance is prepared.

This application relates to a cream for promoting pet hair growth and its preparation method, belonging to the technical field of pet biomedicine. It comprises the following raw materials in weight percentages: microencapsulated fish oil 8%-10%; hemoglobin peptide 6%-12%; silkworm pupa protein powder 3%-5%; hydrolyzed chicken liver powder 2%-5%; yeast extract 1%-3%; rosemary extract 0.005%-0.01%; and antioxidant 0.1%-0.4%. The hemoglobin peptide is selected from one or more of MLL peptides and WDL peptides. The amino acid sequence of the MLL peptide is methionine-leucine-leucine; and the amino acid sequence of the WDL peptide is tryptophan-aspartic acid-leucine. This application has synergistic and long-term stable effects, is suitable for dogs, cats, and other pets, is safe with no side effects, and can be mass-produced.

The invention relates to the technical field of pet heart protection, and discloses a preparation method of a functional health-care product for pet heart and a health-care product of the functional health-care product for pet heart. The preparation method comprises the following steps: mixing 97-99 parts of decanoic acid medium chain triglyceride, 0.5-1.5 parts of astaxanthin and 0.5-1.5 parts of lycopene, and stirring at a certain temperature until the mixture is completely dissolved to obtain a first mixture; s2, placing the obtained first mixture in a high-pressure homogenizer, and homogenizing the first mixture under 90-110 Mpa to prepare a microemulsion; s3, adsorbing the microemulsion into microcapsule particles by using silicon dioxide and corn starch; and S4, uniformly mixing 35-45 parts by mass of the microcapsule particles, 15-25 parts by mass of the chicken liver powder, 2-8 parts by mass of the yeast hydrolysate and 30-40 parts by mass of microcrystalline cellulose, and granulating. The health-care product prepared by the preparation method of the functional health-care product for the pet heart has better effects of relieving inflammation and better absorption.

The invention discloses an application of rutin in reducing ribosome toxicity stress reaction to relieve oxidative stress of poultry livers. Rutin blocks JNK and p38 activation caused by ZAK alpha by down-regulating expression of ribosome toxicity stress reaction key protein ZAK alpha, so that an Nrf2 / Keap1 signal channel is activated, and the effects of anti-oxidation defense and lipid deposition inhibition on liver cells are achieved; according to the invention, rutin with proper concentration is screened out and added into chicken liver cells, and then oxidative damage induction is carried out on the cells, so that the rutin is verified to be capable of preventing oxidative stress generation of the liver cells; meanwhile, after the ZAKalpha is over-expressed in the cells added with the rutin, the protection effect of the rutin is weakened; according to the application of rutin in targeting ribosome stress toxic reaction, a new thought is provided for resisting oxidative stress, and the rutin has important research and development value on selection of development of anti-oxidative stress compounds.

The invention discloses a method for analyzing the influence of salmonella infection on chicken liver transcriptome RNA m6A modification, and relates to the technical field of bioengineering, and the method comprises the following steps: animal model establishment and sample collection, RNA extraction, sequencing and data quality control, bioinformatics analysis and differential m6A peaks identification, and data integration and function enrichment analysis. By integrating MeRIP-seq and a transcriptome sequencing technology, a dynamic regulation and control mechanism of RNA m6A modification in the process of infecting the chicken liver with salmonella is disclosed, an m6A modification and gene expression association analysis framework under an infection model is constructed, the analysis depth of an epigenetic regulation and control mechanism of poultry immune response is remarkably improved, and the application prospect is broad. And by establishing a standardized experimental process and a bioinformatics analysis system, accurate identification of differential modification sites and functional association thereof from a transcriptome level is realized, and a new technical path is provided for poultry disease resistance research.

The invention relates to an avian adenovirus serum 4 type ORF43 gene, a gene-deleted strain constructed by the same and application of the gene-deleted strain. The important gene ORF43 of the FAdV-4, which is obtained by the invention, can be used for remarkably inhibiting the generation of I-type interferon, and an ORF43 gene knockout strain FAdV-4 delta ORF43 is constructed. Compared with a parent strain FAdV-4 ZZ, the FAdV-4 delta ORF43 strain has the advantages that the infection capacity on chicken liver cancer cells is weakened, the in-vitro proliferation speed is slowed down, and the pathogenicity to chickens is reduced. By analyzing the virus load of tissues and organs after the chicken is infected with the FAdV-4 ZZ strain and the FAdV-4 delta ORF43 strain, the virus content of the liver and spleen of the chicken infected with the FAdV-4 delta ORF43 strain is obviously reduced. The FAdV-4 delta ORF43 strain constructed by the invention can be used as a potential vaccine candidate strain, and is used for development and utilization of gene deletion inactivated vaccines or attenuated vaccines.

The invention relates to the technical field of cat food, in particular to a cat food additive for promoting hair care, cat food and a preparation method of the cat food additive. Wherein the cat food additive comprises the following substances: vitamin A, B complex vitamins, vitamin E, zinc methionine, copper methionine, modified astaxanthin, modified L-cysteine, taurine and food-grade soybean lecithin powder; wherein the main materials comprise the following substances: chicken powder, fish dices, chicken liver powder, pea meal, Chinese yam starch, chicken oil, salmon oil, calcium carbonate, table salt and pre-gelatinized radix puerariae powder. The problem that the astaxanthin is inactivated before reaching a target due to crystal surface oxygen adsorption and enrichment in a heterogeneous food matrix is solved.