Virus-eradication and rapid propagation method for lilium pumilum

Abstract

The invention relates to a virus-eradication and rapid propagation method for lilium pumilum. The virus-eradication and rapid propagation method comprises the following steps: selecting capsules, carrying out surface sterilization, culturing ovules, detecting viruses of tissue culture seedlings, carrying out test tube seedling propagation, expanding test tube bulbs and rooting. The virus-free seedlings provided by the invention are obtained without a callus way; the ovules without the viruses are directly induced to form a lot of primary virus-free lilium pumilum test tube seedlings; the method is simple to operate, short in culture period, complete in virus eradication and low in pollution rate; a process from ovule inoculation to sprouting only needs about 40d; the virus eradication ratereaches 100 percent and pollution is basically not caused; wild resources of the lilium pumilum are not damaged. Meanwhile, a regeneration process does not adopts the callus way so that gene mutationis not easy to cause; the reproduction coefficient reaches 7.26 and large-specification test tube bulbs can be obtained within short time; the weight of 91.3 percent of the test tube bulbs exceeds 0.5g; the expanding of the test tube bulbs and the induced rooting are carried out at the same time.

Description

technical field [0001] The invention relates to a rapid propagation method, in particular to a rapid propagation method for detoxification of Shandan, which belongs to the field of tissue culture. Background technique [0002] Shandan ( Lilium pumilum DC.) is a perennial herbaceous plant of the genus Lilium in the family Liliaceae. It has beautiful plant shape, revolving petals, beautiful and delicate flower colors, and is of great ornamental value. Ornamental potted plants. At the same time, Shandan is also an important Chinese herbal medicine. Its underground bulbs have the effects of moistening the lungs and relieving coughs, clearing away heat and calming the nerves, quenching thirst and moistening dryness, preventing and fighting cancer, etc. , has been widely used in many prescriptions. Shandan is an important resistance source material for ornamental and edible lily germplasm innovation due to its strong cold resistance, drought resistance and salt-alkali tolera...

AI Technical Summary

Problems solved by technology

Due to its small bulbs, difficult breeding, complex and diverse native environment, and high virus infection rate, the development of provenance and the preservation of resources in other places are difficult.

Tissue culture technology, as the only effective way for the preservation of Shandan resources and the subsequent industrialization of provenance, has gradually attracted people's attention. However, traditional tissue culture usually requires a large number of excavated underground bulbs of Shandan as explants, and the soil where Shandan bulbs grow There are a large number of microorganisms in the plant. When the scales are used as explants, due to carrying a large number of fungi or bacteria, the pollution rate is extremely high and the success rate is very low during the primary culture, and a large number of Shandan bulbs are excavated as explants. Destruction of Wild Shandan Resources

At the same time, the regenerated offspring obtained from the scales as explants cannot get rid of the virus. As the reproductive generations increase, the virus continues to accumulate, which has a devastating impact on the protection of Shandan resources and the subsequent development of provenance.

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