Virus-eradication and rapid propagation method for lilium pumilum

A Shandan and virus detection technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult breeding, low success rate, high pollution rate, avoid fungal and bacterial pollution, and promote light intensity. , the effect of short training period

Active Publication Date: 2018-12-11
YUNNAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its small bulbs, difficult breeding, complex and diverse native environment, and high virus infection rate, the development of provenance and the preservation of resources in other places are difficult.
Tissue culture technology, as the only effective way for the preservation of Shandan resources and the subsequent industrialization of provenance, has gradually attracted people's attention. However, traditional tissue culture usually requires a large number of excavated underground bulbs of Shandan as explants, and the soil where Shandan bulbs grow There are a large number of microorganisms in the plant. When the scales are used as explants, due to carrying a large number of fungi or bacteria, the pollution rate is extremely high and the success rate is very low during the primary culture, and a large number of Shandan bulbs are excavated as explants. Destruction of Wild Shandan Resources
At the same time, the regenerated offspring obtained from the scales as explants cannot get rid of the virus. As the reproductive generations increase, the virus continues to accumulate, which has a devastating impact on the protection of Shandan resources and the subsequent development of provenance.

Method used

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  • Virus-eradication and rapid propagation method for lilium pumilum
  • Virus-eradication and rapid propagation method for lilium pumilum

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The detoxification and rapid propagation method of Shandan of the present embodiment may further comprise the steps:

[0037]Step (1), Capsule collection: Select the capsules of Shandan that are fully expanded after pollination, immature and yellow, and transported back to the laboratory at a low temperature of 4°C for use;

[0038] Step (2), surface sterilization: Soak the Shandan capsules obtained in step (1) with 0.3% detergent water for 10 minutes, rinse with running water under tap water for half an hour; Soak in 75% alcohol for 60 seconds and shake continuously, then soak in 2.0% sodium hypochlorite solution for 15 minutes, shake continuously during this period, and finally rinse with sterile water 4 times, each time not less than 5 minutes;

[0039] Step (3), ovule culture: place the surface-sterilized Shandan capsules in step (2) on sterile filter paper to absorb water, then cut them longitudinally, and select well-developed, plump and substantial ovules in the ...

Embodiment 2

[0086] The detoxification and rapid propagation method of Shandan of the present embodiment may further comprise the steps:

[0087] Step (1), Capsule collection: Select the capsules of Shandan that are fully expanded after pollination, immature and yellow, and transported back to the laboratory at a low temperature of 4°C for use;

[0088] Step (2), surface sterilization: Soak the Shandan capsules obtained in step (1) with 0.2% detergent water for 20 minutes, rinse with running water under tap water for half an hour; Soak in 70% alcohol for 100 seconds, and keep shaking, then soak in 1.5% sodium hypochlorite solution for 20 minutes, shake constantly, and finally rinse with sterile water 5 times, each time not less than 5 minutes;

[0089] Step (3), ovule culture: place the surface-sterilized Shandan capsules in step (2) on sterile filter paper to absorb water, then cut them longitudinally, and select well-developed, plump and substantial ovules in the upper and middle parts o...

Embodiment 3

[0098] The detoxification and rapid propagation method of Shandan of the present embodiment may further comprise the steps:

[0099] Step (1), Capsule collection: Select the capsules of Shandan that are fully expanded after pollination, immature and yellow, and transported back to the laboratory at a low temperature of 4°C for use;

[0100] Step (2), surface sterilization: Soak the Shandan capsules obtained in step (1) with 0.5% detergent water for 13 minutes, rinse with running water under tap water for half an hour; Soak in 75% alcohol for 100 seconds and shake continuously, then soak in 2.5% sodium hypochlorite solution for 30 minutes, shake continuously during this period, and finally rinse with sterile water 6 times, each time not less than 5 minutes;

[0101] Step (3), ovule culture: Place the surface-sterilized Shandan capsules in step (2) on sterile filter paper to absorb water, then cut them longitudinally, and select well-developed, plump and substantial ovules in th...

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Abstract

The invention relates to a virus-eradication and rapid propagation method for lilium pumilum. The virus-eradication and rapid propagation method comprises the following steps: selecting capsules, carrying out surface sterilization, culturing ovules, detecting viruses of tissue culture seedlings, carrying out test tube seedling propagation, expanding test tube bulbs and rooting. The virus-free seedlings provided by the invention are obtained without a callus way; the ovules without the viruses are directly induced to form a lot of primary virus-free lilium pumilum test tube seedlings; the method is simple to operate, short in culture period, complete in virus eradication and low in pollution rate; a process from ovule inoculation to sprouting only needs about 40d; the virus eradication ratereaches 100 percent and pollution is basically not caused; wild resources of the lilium pumilum are not damaged. Meanwhile, a regeneration process does not adopts the callus way so that gene mutationis not easy to cause; the reproduction coefficient reaches 7.26 and large-specification test tube bulbs can be obtained within short time; the weight of 91.3 percent of the test tube bulbs exceeds 0.5g; the expanding of the test tube bulbs and the induced rooting are carried out at the same time.

Description

technical field [0001] The invention relates to a rapid propagation method, in particular to a rapid propagation method for detoxification of Shandan, which belongs to the field of tissue culture. Background technique [0002] Shandan ( Lilium pumilum DC.) is a perennial herbaceous plant of the genus Lilium in the family Liliaceae. It has beautiful plant shape, revolving petals, beautiful and delicate flower colors, and is of great ornamental value. Ornamental potted plants. At the same time, Shandan is also an important Chinese herbal medicine. Its underground bulbs have the effects of moistening the lungs and relieving coughs, clearing away heat and calming the nerves, quenching thirst and moistening dryness, preventing and fighting cancer, etc. , has been widely used in many prescriptions. Shandan is an important resistance source material for ornamental and edible lily germplasm innovation due to its strong cold resistance, drought resistance and salt-alkali tolera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王有国张永称张小平徐柏林刘金涛
Owner YUNNAN AGRICULTURAL UNIVERSITY
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