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Method for producing detoxified sprout by cultivating garlic stem tip combined with cold treatment

A technology of virus-free seedlings and garlic stems, which is applied in the agricultural field, can solve the problems of increasing costs, increasing cultivation steps, time-consuming and labor-consuming, etc., and achieves the effect of high yield and quality improvement of garlic sprouts

Inactive Publication Date: 2009-02-04
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

The disadvantage of this technology is that it is applied to production after cultivating small garlic bulbs, which increases the cultivation steps, is time-consuming and labor-intensive, and increases the cost.

Method used

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Embodiment Construction

[0014] Provide following embodiment in conjunction with content of the present invention, concrete implementation is as follows:

[0015] 1. Cold storage of garlic cloves Put the harvested garlic in plastic bags, put them in the refrigerator, and store them at a low temperature of 4°C. The processing time is 14 days.

[0016] 2. Sterilization of materials: Place the cold-treated garlic cloves in a tray, add tap water, and culture them in a 30°C incubator for 3 days to break the dormancy of the garlic cloves and induce them to germinate and form seedlings. Choose healthy garlic seedlings, cut off the young leaves and most of the garlic cloves, peel off the garlic skin, rinse with tap water for 2 hours, quickly treat with 70% alcohol on an ultra-clean table for 30 seconds, soak in saturated bleaching semen for 15 minutes, and then rinse with sterile water Rinse 3 times.

[0017] 3. The shoot tip is cultivated on an ultra-clean workbench, the outer scales of the garlic cloves an...

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Abstract

The method for producing detoxified seed seedling by combining garlic shoot tip culture with cold treatment includes the following several steps: (1), cold storage treatment of garlic hop; (2), selecting material and sterilization; (3), shoot tip culture; (4), successive transfer culture; (5), test-tube plantlet rooting; and (6), training plantlet and transplating. Said invention also provides three cuture media for above-mentioned shoot tip culture, successive transfer culture and rooting culture.

Description

technical field [0001] The invention relates to a method in the technical field of agriculture, in particular to a method for cultivating garlic shoot tips to produce virus-free seedlings. Background technique [0002] Garlic is a vegetative propagation crop. In production, it relies on bulbs for asexual reproduction. The accumulation of viruses in the body increases year by year, and the occurrence of viral diseases is serious. According to reports, the yield loss of poisonous garlic can reach 50%. At the same time, the bulbs become smaller, the flower stems turn green, and the shape of the product is greatly affected, making it difficult to meet the consumption requirements of the international market. Infected garlic has small cloves, weak seedlings, slow growth, and narrow leaves. Even if more fertilizers are applied, it is generally difficult to reach the growth rate of large cloves of garlic; thus, the garlic becomes smaller and the unit yield decreases. Garlic virus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H3/00A01C1/00A01G1/00
Inventor 蔡润潘俊松何欢乐
Owner SHANGHAI JIAO TONG UNIV
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