Method for producing detoxified sprout by cultivating garlic stem tip combined with cold treatment

Abstract

The method for producing detoxified seed seedling by combining garlic shoot tip culture with cold treatment includes the following several steps: (1), cold storage treatment of garlic hop; (2), selecting material and sterilization; (3), shoot tip culture; (4), successive transfer culture; (5), test-tube plantlet rooting; and (6), training plantlet and transplating. Said invention also provides three cuture media for above-mentioned shoot tip culture, successive transfer culture and rooting culture.

Description

technical field [0001] The invention relates to a method in the technical field of agriculture, in particular to a method for cultivating garlic shoot tips to produce virus-free seedlings. Background technique [0002] Garlic is a vegetative propagation crop. In production, it relies on bulbs for asexual reproduction. The accumulation of viruses in the body increases year by year, and the occurrence of viral diseases is serious. According to reports, the yield loss of poisonous garlic can reach 50%. At the same time, the bulbs become smaller, the flower stems turn green, and the shape of the product is greatly affected, making it difficult to meet the consumption requirements of the international market. Infected garlic has small cloves, weak seedlings, slow growth, and narrow leaves. Even if more fertilizers are applied, it is generally difficult to reach the growth rate of large cloves of garlic; thus, the garlic becomes smaller and the unit yield decreases. Garlic virus...

AI Technical Summary

Problems solved by technology

The disadvantage of this technology is that it is applied to production after cultivating small garlic bulbs, which increases the cultivation steps, is time-consuming and labor-intensive, and increases the cost.