Method for producing virus-free atractylodes macrocephala koidz seedlings

Abstract

The invention discloses a method for producing virus-free atractylodes macrocephala koidz seedlings. The method comprises the following steps: 1, pre-treating atractylodes macrocephala koidz seeds and aseptically sprouting the embryos atractylodes macrocephala koidz seeds, to be specific, inoculating atractylodes macrocephala koidz embryos into a primary culture medium comprising 1 / 2MS, 20g / L sucrose and 6g / L agar powder; 2, performing proliferation culture, wherein a proliferation culture medium comprises MS, 1.0mg / L 6-BA, 0.2mg / L NAA, 30g / L sucrose and 6g / L agar powder; 3, detecting viruses, to be specific, rejecting virus-containing atractylodes macrocephala koidz seedlings, and continuing to perform proliferation culture on the virus-free atractylodes macrocephala koidz seedlings; 4, performing rooting culture, to be specific, uprightly inserting rootless atractylodes macrocephala koidz tissue culture seedlings which are cultured to about 4cm into a rooting culture medium comprising 1 / 2MS, 1.0mg / L NAA, 20g / L sucrose and 6g / L agar powder; 5, performing acclimatization and transplantation. According to the method, the formulae of the primary culture medium and the rooting culture medium for atractylodes macrocephala koidz are obtained by single-factor tests, the formula of the proliferation culture medium for the atractylodes macrocephala koidz tissue culture seedlings is obtained by an orthogonal design, and most importantly, the virus-free atractylodes macrocephala koidz seedlings are obtained in an embryo culturing way by utilizing the fact that embryo parts are virus-free.

Description

technical field [0001] The invention belongs to the technical field of plant detoxification and tissue culture rapid propagation, and specifically relates to a technical method for producing detoxification seedlings of Atractylodes macrocephala. Background technique [0002] Atractylodes ( Atractylodes macrocephala Koidz), a perennial Asteraceae (Asteraceae) Atractylodes herbaceous plant, is one of the four precious medicinal materials "ginseng, surgery, Ling, sweet" in traditional Chinese medicine. It is a commonly used tonic traditional Chinese medicine, and there is a saying of "ten directions and nine techniques". Due to the wide application of Atractylodes macrocephala and the large market demand, Atractylodes macrocephala has been promoted and planted all over the country. [0003] However, in the cultivation of Atractylodes macrocephala, it is often harmed by diseases and insect pests, especially in high-temperature and high-humidity weather, sometimes the whole pi...

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Problems solved by technology

[0004] The accumulation of viruses in plants greatly affects the quality and yield of plants, causing great harm to the production and utilization of plants

[0005]Conventional shoot tip detoxification is one of the most extensive detoxification methods. In addition to cumbersome operation, there is also a problem of contradiction between detoxification rate and survival rate. That is, if one wants to obtain a higher detoxification rate, the young leaves and other tissues around the shoot tip need to be stripped off, and only the growth cone is left for cultivation, but the survival rate of such materials is very low, sometimes even zero. If one wants to increase the survival rate, then Requires larger shoot tips for cultivation, which in turn leads to suboptimal shedding rates

[0006]In recent years, although there are literatures on the tissue culture and rapid propagation technology of Atractylodes macrocephala, they all belong to conventional tissue culture. Not yet reported

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