59results about How to "High detoxification rate" patented technology

The invention provides a method for rapidly culturing shoot tips in vitro, which is characterized in that in the first step, selecting stolon of the strawberries sprouted at the same year, washing to be clean and disinfecting; in the second step, cutting 0.1-0.5 mm shoot tips of the disinfected and cleaned strawberries stolon under the anatomical lens, inoculating to a culture medium for germination culture, cutting the new sprouts after the 0.5-1.5 cm new sprouts are grown on the shoot tips, inoculating to an enrichment medium for inducing clustered shoots; in the third step, cutting the cluster buds to simple buds, inoculating to a rooting medium for rooting culture; transferring test tube plantlets to a substrate formed by mixing perlite and peat, watering and covering a plastic film for insulation and moisture retention, revealing the plastic film for gradually hardening, transferring the seedling to a plastic potted tray filled with pure peat, watering and covering the plastic film for insulation and moisture retention, and directly planting the seedling to a field. The invention has the advantages of high reproductive rate, rapid reproduction speed and high survival rate.

The invention relates to 'a method for removing strawberry mild yellow edge virus (SMYEV) by the ultralow temperature technique', which belongs to the field of bioengineering. During the virus removal process, the concentration of pre-cultured sucrose is 0.5 mol per liter, and 3d is treated; the 3d is subjected to loading treatment for 60 minutes at a temperature of 25 DEG C; the 3d is subjected to vitrification treatment for 120 minutes at a temperature of 0 DEG C; and the 3d is subjected to liquid nitrogen treatment for 60 minutes and water bath treatment for 2 minutes at a temperature of 40 DEG C, wherein the survival rate of strawberry stem tips is 76 percent, and the detoxication rate of the strawberry mild yellow edge virus reaches 95 percent. The virus can be only removed by means of the liquid nitrogen treatment, and the detoxication rate of the vitrification treatment before the liquid nitrogen treatment is 0 percent. Compared with the prior detoxication method such as the stem tip culture detoxication method, the heat treatment detoxication method, and so on, the ultralow temperature detoxication method not only has quite high detoxication rate, but also is simple and feasible, convenient to operate, and does not require expensive instruments, so as to provide strong technical support for demonstration and popularization and industrialization of nontoxic strawberry seedlings.

The invention relates to a method for rapid removing three sorts of virus in lily, belonging to biologic technical field, which is characterized in that: the meristematic tissue of stem tip is inoculated in the culture medium with ribavirin; wherein, the size of the stem tip is 0.5 to 0.8mm, and the concentration of the ribavirin is 4 mg/L to 6 mg/L. The invention has the advantages of high detoxication rate, high survival rate and rapid regeneration speed.

The invention provides a new method for detoxifying a rape-seed meal by fermenting saccharomycetes and lactic acid bacteria together. The rape-seed meal has been taken as a feed additive for a long time, but the rape-seed meal cannot be used before being detoxified because the rape-seed meal contains constituents, such as glucosinolate and the like, which are harmful to livestock. Microbial fermentation is a novel detoxification method in conventional rape-seed meal detoxification technology and can overcome the defects of small detoxification range, low detoxification rate, poor palatability of detoxified rape-seed meal and the like in physical and chemical detoxification methods. In the invention, glucosinolate in the rape-seed meal is removed by fermenting saccharomycetes and lactic acid bacteria together; after saccharomycetes and lactic acid bacteria are fermented together in a ratio of 1:1 for 3 to 7 days, the detoxification rate can reach about 60 percent, glucosinolate content can be lowered to about 0.15 percent and protein content of the rape-seed meal is remarkably increased due to microbial fermentation.

The invention discloses a culture method of a virus-free test-tube corm of Fanhong No.1 crocus sativus l. The culture method comprises the following steps: soaking a crocus sativus l. corm with warm water, then carrying out water culture germination acceleration, taking a lateral bud of the corm, culturing, stripping to take a shoot tip meristem on the obtained aseptic seedling, culturing, taking the seedling as a plant I when the seedling grows to the height of 1.5 cm-2.0 cm, carrying out bean yellow mosaic virus particle detection on the plant I, if virus particles are detected, stripping to take a shoot tip meristem from the plant I to replace the shoot tip meristem stripped from the aseptic seedling in the former step, and repeating the steps until the plant I containing no bean yellow mosaic virus particles is obtained; culturing the plant I containing no bean yellow mosaic virus particles; and inoculating a corm induced expansion culture medium with induced cluster buds, and culturing until the buds grow to required size. The method can produce a large amount of high-quality virus-free healthy seedlings with the same genetic background in a short time.

The invention discloses a ginger virus-free breeding method. The ginger virus-free breeding method comprises the following steps: (1) preparing ginger virus-free seedlings; (2) amplifying reproductionof the ginger virus-free seedlings; and (3) transplanting and domesticating, namely transplanting a large number of ginger virus-free seedlings obtained in the step (2) into a culture medium, and culturing for 1-2 weeks at the temperature of 20-28 DEG C, wherein the culture medium is a mixture obtained by mixing perlite, peat soil and a biological bacterial fertilizer in a volume ratio of (1-2):(3-5):(0.3-0.8), concentrations of nitrogen, phosphorus and potassium in earlier stage culture are 60-100mg/L, 20-40mg/L and 100-150mg/L respectively, concentrations of nitrogen, phosphorus and potassium in later stage are 200-220mg/L, 20-40mg/L and 200-250mg/L respectively, and a fertilizer is applied once a week. The ginger virus-free breeding method disclosed by the invention has the advantagesthat various parameters of the whole breeding process are studied, the optimized parameters are combined together, and virus-free percent, survival rate and yield of ginger can be effectively improvedwhile breeding period of the ginger is shortened.

The invention provides a method of removing virus for Jiangxi Qianshan red-bud taro through vitrification cryopreservation. The method comprises: (1) cutting shoot tips with a length being about 1 mm of single buds of the red-bud taro under a microscope; (2) pre-culturing the shoot tips on a solid medium of MS + KT 2 mg/L + NAA 0.5 mg/L +0.75 M sucrose for three days; (3) after the shoot tips are pre-cultured, using a 60 % PVS2 to dehydrate for 60 minutes under 25 DEG C, then using a 100 % PVS2 to dehydrate for 90 minutes under 0 DEG C, replacing with a new PVS2, and then putting into liquid nitrogen; (4) after preservation, defrosting the shoot tips in a 37 DEG C water bath, washing with a liquid medium of MS + KT 2 mg/L + NAA 0.5 mg/L +1.2 M sucrose for 3 times, 10 minutes for each time, culturing the washed shoot tips in dark for 5 days under 25 DEG C, and then culturing under a normal photoperiod with an illumination time being 14 hours per day, a light intensity being about 1500-2000lx, and a culturing temperature being 25 DEG C, wherein the shoot tips can grow into normal buds after about 15 days; and (5) culturing the germinated buds in a rooting medium of 1/2 MS + NAA 0.1-0.5 mg/L, thereby obtaining the virus-free seedlings of the red-bud taro. Compared with prior art, the tube-test plantlets of the red-bud taro are high in virus removing rate, the shoot tips can be taken in a large amount and the operation is simple and convenient.

The invention discloses a potato stem tip detoxification method which includes the following steps: A1 sowing pre-basic seed to be detoxified in a field; A2 conducting normal management in the plant growing period; A3 selecting and marking healthy plants from the potato field in the budding period or the flowering period, harvesting the marked plants half a month earlier than other plants and selecting a large-size standard-type healthy potato seed of each plant to be singly harvested and stored; A4 shearing buds to conduct virus detection after the potato seed sprouts; A5 conducting sprouting on the detected healthy potato seeds, shearing large buds which are subjected to alcohol and corrosive sublimate sterilization processing and placing the buds on a common MS solid medium to produce stem seedlings; A6 shearing 1/3 upper parts of the growing stem seedlings to conduct breed conservation and conducting virus detection on the surplus 2/3 parts; and A7 conducting rapid propagation according to a common method after virus detection is qualified. By means of the potato stem tip detoxification method, potato detoxification test tube seedlings can be obtained in a short period, and the obtained detoxification test tube seedlings are healthy and stable in heredity gene.

The invention relates to an efficient detoxification tissue cultivating method of strawberries, which takes the micro stem tips at the top of strawberry stolons as explants to conduct tissue cultivating to obtain a strawberry plant. According to the method provided by the invention, the germination rate of the micro stem tips is above 95 percent, and rooting percentage is above 98 percent. The method conducts rapid propagation by using the micro stem tips, the propagation coefficient is higher, original variety character is well kept, and the application prospect of new superior varieties strawberry plant short-term expanding propagation and rapid propagation of plants saved in a strawberry genebank is wide; furthermore, the method can be used as a new transgenosis way.

The invention provides a tissue culture method for rapid detoxification of purple potatoes. The method includes the following steps that 1, bud stems of the purple potatoes are cut; 2, the cut bud stems are subjected to sterile water cleaning, instantaneous alcohol disinfection, disinfectant soaking disinfection and sterile water washing in sequence, and disinfected bud stems are obtained; 3, the disinfected bud stems are placed on an ultra-clean bench, stem tips at the top ends of the bud stems are stripped under a microscope and then inoculated in a culture medium for primary tissue culture, and primary test-tube plantlets are obtained; 4, stem tips of the primary test-tube plantlets are stripped, secondary tissue culture is performed, and secondary test-tube plantlets are obtained; 5, stripping and tissue culture in the step 4 are repeatedly preformed 3-5 times, and detoxified test-tube plantlets are obtained. The detoxification rate of the purple potatoes cultured with the method reaches up to 93%, the reproduction cycle is shortened by 2-4 months, batched and rapid reproduction of the purple potatoes can be achieved, and the culture cost can be lowered.

A heat treating method for detoxicating the tissue-cultured seedlings of lily features that the lily bulb in germination state is treated at 35-50 deg.C and 100% air humidity for 20-40 days. Its advantages are high survival rate and high detoxicating effect.

The invention provides a comprehensive detoxification method of lily bulbs. The method concretely comprises the following steps: 1, picking off explant growing points; 2, carrying out heat treatment detoxification; 3, cleaning and disinfecting explants; 4, and carrying out collection and induction culture on the growing pints; 5, carrying out detoxifying culture; and 6, detecting viruses. The method solves disadvantages in the prior art, solves the disadvantages of individual methods through comprehensively utilizing heat treatment detoxification, meristem culture detoxification and callus detoxification methods, and also has the advantages of effective reduction of the virus cross infection change, high detoxification rate, high survival rate, and effective shortening of the bulb culture period.

The invention discloses a method for detoxifying radix tetrastigme through vitrification ultralow temperature therapy, and belongs to the technical field of plant detoxification. The method comprises the following specific steps that (1), aseptic seedlings of radix tetrastigme is pre-cultured; (2), stem tips of the aseptic seedlings of the radix tetrastigme pre-cultured in the step (1) are cut, and differentiation culture is carried out to obtain seedlings of the radix tetrastigme; (3), stem tips of the radix tetrastigme seedlings obtained in the step (2) are cut, the stem tips are loaded by using a PVS2 solution with the concentration of 50%-60%, the stem tips are dehydrated by using a PVS2 solution containing nano calcium carbonate, and a container filled with the PVS2 solution and the stem tips are frozen at ultralow temperature; and (4), the stem tips frozen at the ultra-low temperature in the step (3) is thawed, and then recovery culture is carried out to obtain the detoxified seedlings of the radix tetrastigme. The stem tips of the radix tetrastigme treated by the detoxification method of the vitrification ultralow temperature therapy are high in survival rate, and the detoxification rate of survived seedlings is further high.

The invention provides a potato stem tip detoxication method, which comprises the following steps of A1, selecting healthy plants for field marking; selecting big and healthy seed potatoes with standard potato shapes; breaking the dormancy of the tubers of the seed potatoes; then, performing heat treatment for 6 weeks; spraying salicylic acid twice in the period; A2, peeling big sprouts from the potato blocks obtained in the step A1; performing cleaning; A3, under the sterile conditions, cutting 1 to 3mm of the top of the big sprouts; inoculating a liquid culture medium; culturing the big sprouts into test-tube seedlings. The method has the advantages that the stem tip peeling step is simplified; the stem tip peeling technology difficulty is obviously reduced, so that the regenerated plants become strong seedlings from weak seedlings; the conventional multi-step seedling formation is changed into one-step seedling forming; the seedling forming time is about 30 days; the virus elimination period is shortened by about 33.3 percent to 60 percent; the crossing of long period to short period from the potato blocks to virus-free seedlings is realized; the seedling forming rate is increased by 44 percent or more; the current condition of stem tip peeling seedling forming difficulty is changed; the virus elimination rate is improved by 36 percent or higher; the technical bottleneck ofdifficult elimination of PVS and PVM by a conventional potato virus elimination method is broken.

The invention discloses a detoxification method of lily bulbs, wherein the method comprises the following steps: (1) placing the lily bulbs for 40-50 days at the temperature of 3-8 DEG C; (2) selecting the lily bulbs having the bud length of 4-8 cm, stripping outer-layer flakes, leaving bulbus discs with 3-5 inner-layer flakes, placing the bulbus discs into an incubator, and preprocessing, wherein the preprocessing conditions comprise that the illumination intensity is 1500-2000 Lux, the temperature is 35-40 DEG C and the processing time is 2-5 days; (3) selecting a normally growing bulbus disc, stripping a 0.4-0.7 cm stem tip, transferring into a differentiation culture medium, firstly, carrying out dark culture for 1-2 days at the temperature of 23-25 DEG C, then transferring into an illumination incubator with the illumination intensity of 1500-2000 Lux and the illumination time of 12-16 h/d, heating up at a speed of 1-5 DEG C/d, rising the temperature to 35-45 DEG C, and then keeping the temperature and culturing for 35-45 days. Lily bulb viruses are removed by combining heat treatment and chemical treatment, the induction culture period is shortened, and the method also has the advantages of high detoxification rate, high value-added coefficient and the like, and provides a technical basis for culture of lily virus-free plants and promotion of virus-free culture research.

The invention discloses a method for detoxifying colocasia esculenta by droplet vitrification ultra-low-temperature therapy, and belongs to the technical field of agricultural production. The method comprises the following steps: pretreating stem tips of the colocasia esculenta by a gradient heating method; taking 0.5-1.5 cm of stem tips, transferring the stem tips to a basic culture medium, and performing illumination culture for 1-3 days; transferring the stem tips into a sugar-containing culture medium, loading for 20-30 min, performing dehydrating for 10-20 min by using PVS2, and preserving the stem tips for 1-2 days in liquid nitrogen; after unfreezing, washing the stem tips with a washing solution, then transferring the stem tips into a solid culture medium for dark culture for 2-3 days, and then conducting culture for 20-30 days under an illumination condition to differentiate new buds; and transferring the new buds into a solid culture medium for culturing to obtain virus-free seedlings of the colocasia esculenta. According to the method, after the stem tips of the colocasia esculenta are treated through gradient heating and an embedding medium, primary detoxification can be achieved, the high differentiation capacity of stem tip cells is kept, further detoxification is achieved in combination with a droplet vitrification method and ultralow-temperature treatment, and the detoxification rate and the survival rate of virus-free seedlings can be increased.

The invention discloses a method for rapid propagation of ginger by tissue culture. The method is characterized by comprising the following steps of taking ginger stem tips to be pretreated, placing the pretreated ginger stem tips on a slow-release culture medium, inducing to generate cluster buds, adding an MS basal culture medium with NH4NO3 concentration halved into the slow-release culture medium, supplementing 0.5-1 mg/L of PP333, and continuously culturing to obtain rooted seedlings, wherein the slow-release culture medium is prepared by supplementing 0.5-1.5 mg/L of 6-BA, 2-3mg/L of NAA slow-release auxin, 0.25-0.3 mg/L of TDZ, 2% of cane sugar and 0.55-0.65% of agar into an MS basal culture medium, and the pH value is adjusted to 5.8-6. The tissue culture steps are simplified, the culture time is shortened, the survival rate and detoxification rate of bud seedlings are increased, the survival rate reaches 96%, the detoxification rate reaches 100%, meanwhile, variation in the ginger tissue culture process is prevented, the propagation coefficient is remarkably increased and reaches 5.13, the rooting rate reaches 97.7%, and robust ginger seedlings with roots are cultured.

The invention provides a method for preserving and purifying smallanthus sonchifolius germplasms based on an ultra-low temperature technology, and relates to the field of agricultural biotechnology. Atechnical scheme of the invention is composed of the following steps: S1, obtaining of smallanthus sonchifolius clone; S2, low-temperature acclimation; S3, infiltration embedding; S4, dehydration andliquid nitrogen preservation; S5, thawing and washing; and S6, plant regeneration. The germplasm preservation method provided by the invention is simple and feasible, high in stability, and capable of being stored for a long time after storage at a time; a survival rate of stored stem tips is still higher than 80%; a plant regeneration rate is up to 75% or more; meanwhile, the method provided bythe invention further can detoxify and purify smallanthus sonchifolius germplasm resources to improve the quality of seedlings and provide more high-quality detoxified seedlings for production, and has broad market prospects.

The invention belongs to the technical field of oil meal crop detoxification, and specifically to a detoxification method of castor meal. According to the present invention, the oil-extracted castor meal is adopted as a raw material, and the nontoxic castor meal is obtained through physical crushing, conditions of high temperature and negative pressure in the internal of a reactor, a mechanical stirring treatment and other processes, wherein the nontoxic castor meal can be used as a protein additive in the feed. After testing, the crude protein content in the detoxified castor meal is 36-40%; the amino acid components in the castor meal crude protein mainly comprise 20.05% of Glu, 10.97% of Arg, 9.64% of Asp, 7.81% of Leu, 2.24% of Lys, and 1.07% of Met; the animal digestible hemicellulose content is 20%; and the ricinine content is less than or equal to 0.006%. The results of the feeding experiments show that the detoxified castor meal of the present invention can be properly and safely used for pig feeds, and aquatic feeds of loach, rice field eel and the like.