59results about How to "High detoxification rate" patented technology

The invention provides a method for reproducing purple yam seedlings by tissue culture. The method takes a purple yam tender stem tip of 1.0cm as an explant and comprises the following steps: sterilizing the explant by 75% alcohol and a 0.1% mercuric chloride solution; inoculating the explant on an initial culture medium MS0; after the stem tip is survived, treating a regenerated tender stem tip of 0.5-1.0 mm; carrying out stem tip induction culture, stem tip subculture multiplication culture and rooting culture with different culture media at different periods to obtain a purple yam bottle seedling; after the seedling height is about 2cm, transplanting the bottle seedling into a nutrition bowl substrate for hardening; and culturing for 50-60 days till the seedling grows to be as high as 10cm, thereby obtaining the purple yam seedling. A stem tip induction multiplication culture medium is formed by MS, 0.5mg / L of 6BA and 0.1mg / L of NAA; a rooting culture medium is formed by 1 / 2MS, 0.2mg / L of NAA and 0.2g / L of Ac; each culture medium contains 45g / L of edible sugar and 6g / L of agar, and the pH of each culture medium is 5.7. According to the method, a germination accelerating manner is simple and convenient and the germination speed is high; a detoxification manner is simple and rapid and the detoxification rate is high; the variety is high in quality and pure; with the method, the industrialized production of purple yam tissue culture seedlings and the popularization of breed improvement can be realized.

The invention discloses a ginger virus-free breeding method. The ginger virus-free breeding method comprises the following steps: (1) preparing ginger virus-free seedlings; (2) amplifying reproductionof the ginger virus-free seedlings; and (3) transplanting and domesticating, namely transplanting a large number of ginger virus-free seedlings obtained in the step (2) into a culture medium, and culturing for 1-2 weeks at the temperature of 20-28 DEG C, wherein the culture medium is a mixture obtained by mixing perlite, peat soil and a biological bacterial fertilizer in a volume ratio of (1-2):(3-5):(0.3-0.8), concentrations of nitrogen, phosphorus and potassium in earlier stage culture are 60-100mg / L, 20-40mg / L and 100-150mg / L respectively, concentrations of nitrogen, phosphorus and potassium in later stage are 200-220mg / L, 20-40mg / L and 200-250mg / L respectively, and a fertilizer is applied once a week. The ginger virus-free breeding method disclosed by the invention has the advantagesthat various parameters of the whole breeding process are studied, the optimized parameters are combined together, and virus-free percent, survival rate and yield of ginger can be effectively improvedwhile breeding period of the ginger is shortened.

The invention relates to the technical field of moth orchid seedling breeding, in particular to a method for cultivating and propagating moth orchid virus-free seedlings, which specifically comprises the following steps: selecting robust and virus-carrying moth orchid plants, soaking them in chitosan solution after disinfection, and then carrying out For the primary culture, the virus-negative clustered shoots obtained in the primary culture are selected for subculture, and finally the seedlings with good growth obtained after the subculture are selected to continue the cultivation of strong seedlings, rooting culture and transplanting. The method for cultivating and propagating the virus-free seedlings of the Phalaenopsis provided by the invention can obtain the Phalaenopsis seedlings with good quality, high virus-free rate and robust growth, and realizes virus-free cultivation of the Phalaenopsis.