Culture method of virus-free test-tube corm of Fanhong No.1 crocus sativus l

A technology of saffron and culture method, which is applied in the field of tissue culture of saffron detoxification test tube bulbs, and can solve the problems affecting the development of saffron medicinal material industry, the degradation of saffron quality material germplasm, and the infection of bulb virus diseases, etc. problem, to achieve the effect of increasing the acquisition, promoting the formation, increasing the C source and energy

Active Publication Date: 2016-03-02
ZHEJIANG UNIV
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Problems solved by technology

[0003] The stigma of saffron is only used as medicine, and the wild resources are limited. Jiande, Zhejiang is one of the main cultivation places. For a long time, the asexual reproduction method of bulb breeding has been used for reproduction. promotion of improved varieties
In addition, due to years of planting, the bulbs have been seriously infected by virus diseases, mainly Beanyellowmosaic virus (BYMV), which has led to the degradation of the quality of saffron germplasm and the decline in quality. These factors have seriously affected the development of the saffron medicinal material industry.

Method used

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  • Culture method of virus-free test-tube corm of Fanhong No.1 crocus sativus l
  • Culture method of virus-free test-tube corm of Fanhong No.1 crocus sativus l

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Embodiment 1

[0039] Embodiment 1, a kind of tissue culture method of saffron test tube corm, carry out following steps successively:

[0040] 1) Soak the robust and plump saffron bulbs without disease spots and galls in warm water with a water temperature of 55-65°C for 30 minutes, and then dry them in the air (i.e., place them at room temperature until there are no obvious water droplets on the surface of the saffron bulbs. ), then soak in warm water at 55-65°C; repeat the above-mentioned drying and soaking for 2 times, then carry out hydroponic germination; the culture conditions are: dark culture at 26-28°C;

[0041] 2) When the side buds of the corm germinate and grow to a height of ≥0.5cm, gently shake the side buds, wipe off the entire side buds even at the base, and after routine disinfection of the above side buds, cut out 0.2cm to 0.5cm of the base and inoculate them on the growth medium Cultivate to obtain sterile seedlings; culture conditions are: 16 hours of light, light intens...

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Abstract

The invention discloses a culture method of a virus-free test-tube corm of Fanhong No.1 crocus sativus l. The culture method comprises the following steps: soaking a crocus sativus l. corm with warm water, then carrying out water culture germination acceleration, taking a lateral bud of the corm, culturing, stripping to take a shoot tip meristem on the obtained aseptic seedling, culturing, taking the seedling as a plant I when the seedling grows to the height of 1.5 cm-2.0 cm, carrying out bean yellow mosaic virus particle detection on the plant I, if virus particles are detected, stripping to take a shoot tip meristem from the plant I to replace the shoot tip meristem stripped from the aseptic seedling in the former step, and repeating the steps until the plant I containing no bean yellow mosaic virus particles is obtained; culturing the plant I containing no bean yellow mosaic virus particles; and inoculating a corm induced expansion culture medium with induced cluster buds, and culturing until the buds grow to required size. The method can produce a large amount of high-quality virus-free healthy seedlings with the same genetic background in a short time.

Description

technical field [0001] The invention relates to a tissue culture method for detoxified test tube bulbs of saffron. Background technique [0002] Saffron (Crocussativus L.), also known as saffron and saffron, is a perennial plant of the Iridaceae genus Crocus. And the reputation of "the most high-end spices" has important economic value. The Chinese Pharmacopoeia 2010 Edition (Part One) records that saffron is sweet in taste, flat in nature, heart-guiding, and liver meridian; it can promote blood circulation and remove blood stasis, cool blood, detoxify, relieve stagnation and calm the nerves; it is used for amenorrhea, postpartum stasis, and warm toxin Spots, melancholy ruffian, palpitations and madness and other diseases. In recent years, it has also been found that crocetin has anticancer effects. [0003] The stigma of saffron is only used as medicine, and the wild resources are limited. Jiande, Zhejiang is one of the main cultivation places. For a long time, the asexu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 毛碧增吴李芳董峰丽
Owner ZHEJIANG UNIV
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