Efficient detoxification tissue cultivating method of strawberries

Abstract

The invention relates to an efficient detoxification tissue cultivating method of strawberries, which takes the micro stem tips at the top of strawberry stolons as explants to conduct tissue cultivating to obtain a strawberry plant. According to the method provided by the invention, the germination rate of the micro stem tips is above 95 percent, and rooting percentage is above 98 percent. The method conducts rapid propagation by using the micro stem tips, the propagation coefficient is higher, original variety character is well kept, and the application prospect of new superior varieties strawberry plant short-term expanding propagation and rapid propagation of plants saved in a strawberry genebank is wide; furthermore, the method can be used as a new transgenosis way.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a high-efficiency detoxification tissue culture method for strawberries. Background technique [0002] Strawberry is a perennial herbaceous plant, which mainly relies on stolons for vegetative reproduction in cultivation and production. After many years of planting, due to infection with various viruses, the quality and yield are reduced, and the economic benefits are significantly reduced. Strawberry virus-free tissue culture seedlings have been widely used in agricultural production. It plays a pivotal role in reducing production costs, improving the quality of strawberry products, and improving farmers' economic income. Using tissue culture and rapid propagation technology, the virus-free strawberry seedlings can be rapidly propagated and promoted. The seedlings emerge neatly, and can be used for annual production without seasonal restrictions. It can realize industrial se...

AI Technical Summary

Problems solved by technology

For example, if the shoot tip is used as explant culture, there are mainly problems of low detoxification rate and low reproduction coefficient; while using leaves, petioles, roots, anthers, petals, stipules, ovary, etc. as explants, although the detoxification rate And the reproduction coefficient has been greatly improved, but there is a problem of large variation, and it cannot maintain the original breed of the original variety.