Efficient detoxification tissue cultivating method of strawberries

A strawberry, high-efficiency technology, applied in the field of plant tissue culture, can solve the problems of poor maintenance of seed, low reproduction coefficient, and low detoxification rate, and achieve the goal of reducing the cultivation period, high reproduction coefficient, and high detoxification rate Effect

CN103348918AInactive Publication Date: 2013-10-16合肥瑞谷农业科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2013-10-16
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The invention relates to an efficient detoxification tissue cultivating method of strawberries, which takes the micro stem tips at the top of strawberry stolons as explants to conduct tissue cultivating to obtain a strawberry plant. According to the method provided by the invention, the germination rate of the micro stem tips is above 95 percent, and rooting percentage is above 98 percent. The method conducts rapid propagation by using the micro stem tips, the propagation coefficient is higher, original variety character is well kept, and the application prospect of new superior varieties strawberry plant short-term expanding propagation and rapid propagation of plants saved in a strawberry genebank is wide; furthermore, the method can be used as a new transgenosis way.
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Description

technical field

[0001] The invention relates to the field of plant tissue culture, in particular to a high-efficiency detoxification tissue culture method for strawberries. Background technique

[0002] Strawberry is a perennial herbaceous plant, which mainly relies on stolons for vegetative reproduction in cultivation and production. After many years of planting, due to infection with various viruses, the quality and yield are reduced, and the economic benefits are significantly reduced. Strawberry virus-free tissue culture seedlings have been widely used in agricultural production. It plays a pivotal role in reducing production costs, improving the quality of strawberry products, and improving farmers' economic income. Using tissue culture and rapid propagation technology, the virus-free strawberry seedlings can be rapidly propagated and promoted. The seedlings emerge neatly, and can be used for annual production without seasonal restrictions. It can realize industrial se...

Examples

Embodiment 1

[0021] (1) Prepare medium:

[0022] 1. The solvent of MS basic culture medium is water and the solute is shown in Table 1.

[0023] Table 1. Solutes of MS basic culture medium

[0024] Macronutrients

Concentration in medium (g / L)

NH4NO3

1.65

KNO3

1.9

CaCl2

0.33

MgSO4 7H2O

0.37

KH2PO4

0.17

MES

0.5

trace elements

Concentration in medium (mg / L)

MnSO4 4H2O

22.3

ZnSO4 7H2O

8.6

H3BO3

6.2

KI

0.83

Na2MoO4 2H2O

0.25

CuSO4 5H2O

0.025

CoCl2 6H2O

0.025

iron salt

Concentration in medium (mg / L)

Na2-EDTA

27.8

FeSO4 7H2O

37.3

organic ingredients

Concentration in medium (mg / L)

Inositol

100

Glycine

2.0

VB1

0.1

VB6

0.5

Niacin

0.5

[0025] 2. The solvent of the 1 / 2MS basic culture medium is water, and the solutes are shown in Ta...