Ginger tissue culture method

Abstract

The invention discloses a method for rapid propagation of ginger by tissue culture. The method is characterized by comprising the following steps of taking ginger stem tips to be pretreated, placing the pretreated ginger stem tips on a slow-release culture medium, inducing to generate cluster buds, adding an MS basal culture medium with NH4NO3 concentration halved into the slow-release culture medium, supplementing 0.5-1 mg / L of PP333, and continuously culturing to obtain rooted seedlings, wherein the slow-release culture medium is prepared by supplementing 0.5-1.5 mg / L of 6-BA, 2-3mg / L of NAA slow-release auxin, 0.25-0.3 mg / L of TDZ, 2% of cane sugar and 0.55-0.65% of agar into an MS basal culture medium, and the pH value is adjusted to 5.8-6. The tissue culture steps are simplified, the culture time is shortened, the survival rate and detoxification rate of bud seedlings are increased, the survival rate reaches 96%, the detoxification rate reaches 100%, meanwhile, variation in the ginger tissue culture process is prevented, the propagation coefficient is remarkably increased and reaches 5.13, the rooting rate reaches 97.7%, and robust ginger seedlings with roots are cultured.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of ginger. Background technique [0002] Ginger is a perennial herbaceous plant of the Zingiberaceae Zingiberaceae that lives for one year, and is the main source of planting in many areas of my country. Ginger is an economical plant with both medicinal and edible uses. It has the functions of sweating and relieving the exterior, warming the middle and relieving vomiting, warming the lungs and relieving cough, and detoxifying fish and crabs. China is the birthplace and one of the main producing countries of ginger, with a cultivation history of more than two thousand years. The traditional ginger production is to select strong, disease-free rhizomes in the harvest season and store them in a cellar. The next year, the germinated ginger is cut into pieces for planting. This method has serious waste and requires a lot of manpower and material reso...

AI Technical Summary

Problems solved by technology

But existing tissue culture is not suitable for the tissue culture of ginger, it is difficult to induce it to produce callus tissue and clustered buds, slow propagation, easy browning of callus tissue, low induction survival rate, easy rotten seedlings, and loaded down with trivial details Variations are prone to occur during the cultivation process, resulting in problems such as low reproduction coefficient, low rooting rate, and deformed seedlings in ginger tissue culture.

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