Ginger tissue culture method

A tissue culture, ginger technology, applied in the field of ginger tissue culture, can solve the problems of low reproduction coefficient, slow reproduction, low rooting rate and the like

Active Publication Date: 2021-04-27
重庆市幅沅农业生物技术研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But existing tissue culture is not suitable for the tissue culture of ginger, it is difficult to induce it to produce callus tissue and clustered buds, slow propagation, easy browning of callus tissue, low induction survival rate, easy r...

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  • Ginger tissue culture method
  • Ginger tissue culture method

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Experimental program
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Effect test

Embodiment 1

[0029] A method for rapid propagation of ginger by tissue culture, characterized in that, proceed as follows:

[0030]Pretreatment: take a ginger stem tip with a size of 0.6 cm, infiltrate it with ethanol with a volume concentration of 70% for 30 seconds, then wash it with sterile water, repeat the same ethanol infiltration once, wash it with sterile water, and heat it at 50°C 5min;

[0031] Inoculation

[0032] (1) Inoculate ginger shoot tips after pretreatment in slow-release medium and carry out primary culture, and described slow-release medium is to supplement 6-BA 0.5mg / L, NAA slow-release auxin 2mg / L in MS basal medium , TDZ 0.3mg / L, 2% sucrose, agar 0.55%, adjust the pH to 5.8;

[0033] Among them, NAA sustained-release auxin is made of nano-SiO 2 Disperse in de-ethanol ionized aqueous solution, add NAA, then add PVP k30, mix and heat to 80°C for 30 minutes, stop heating, cool to room temperature, then filter and dry to obtain SiO 2 , The mass ratio of NAA and PVP ...

Embodiment 2

[0036] A method for rapid propagation of ginger by tissue culture, characterized in that, proceed as follows:

[0037] Pretreatment: take a ginger stem tip with a size of 0.8 cm, soak it with ethanol with a volume concentration of 70% for 40 seconds, then wash it with sterile water, repeat the same soaking with ethanol once, wash it with sterile water, and heat it at 50°C 8min;

[0038] Inoculation

[0039] (1) Inoculate ginger shoot tip after pretreatment in slow-release medium and carry out primary culture, and described slow-release medium is to supplement 6-BA 1.5mg / L, NAA slow-release auxin 3mg / L in MS basal medium , TDZ 0.25mg / L, 2% sucrose, agar 0.65%, adjust the pH to 6;

[0040] Among them, NAA sustained-release auxin is made of nano-SiO 2 Disperse in de-ethanol ionized aqueous solution, add NAA, then add PVP k30, mix and heat to 90°C for 20 minutes, stop heating, cool to room temperature, then filter and dry to obtain SiO 2 , The mass ratio of NAA and PVP k30 is ...

Embodiment 3

[0043] A method for rapid propagation of ginger by tissue culture, characterized in that, proceed as follows:

[0044] Pretreatment: take a ginger stem tip with a size of 0.7 cm, soak it with ethanol with a volume concentration of 70% for 35 seconds, then wash it with sterile water, repeat the same soaking with ethanol once, wash it with sterile water, and heat it at 50°C 6min;

[0045] Inoculation

[0046] (1) Ginger stem tip after pretreatment is inoculated in slow-release medium and carries out primary culture, and described slow-release medium is to supplement 6-BA 1mg / L, NAA slow-release auxin 2.5mg / L in MS basal medium , TDZ 0.28mg / L, 2% sucrose, agar 0.6%, adjust the pH to 5.8;

[0047] Among them, NAA sustained-release auxin is made of nano-SiO 2 Disperse in de-ethanol ionized aqueous solution, add NAA, then add PVP k30, mix and heat to 85°C for 25min, stop heating, cool to room temperature, then filter and dry to obtain SiO 2 , The mass ratio of NAA and PVP k30 is...

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PUM

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Abstract

The invention discloses a method for rapid propagation of ginger by tissue culture. The method is characterized by comprising the following steps of taking ginger stem tips to be pretreated, placing the pretreated ginger stem tips on a slow-release culture medium, inducing to generate cluster buds, adding an MS basal culture medium with NH4NO3 concentration halved into the slow-release culture medium, supplementing 0.5-1 mg/L of PP333, and continuously culturing to obtain rooted seedlings, wherein the slow-release culture medium is prepared by supplementing 0.5-1.5 mg/L of 6-BA, 2-3mg/L of NAA slow-release auxin, 0.25-0.3 mg/L of TDZ, 2% of cane sugar and 0.55-0.65% of agar into an MS basal culture medium, and the pH value is adjusted to 5.8-6. The tissue culture steps are simplified, the culture time is shortened, the survival rate and detoxification rate of bud seedlings are increased, the survival rate reaches 96%, the detoxification rate reaches 100%, meanwhile, variation in the ginger tissue culture process is prevented, the propagation coefficient is remarkably increased and reaches 5.13, the rooting rate reaches 97.7%, and robust ginger seedlings with roots are cultured.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of ginger. Background technique [0002] Ginger is a perennial herbaceous plant of the Zingiberaceae Zingiberaceae that lives for one year, and is the main source of planting in many areas of my country. Ginger is an economical plant with both medicinal and edible uses. It has the functions of sweating and relieving the exterior, warming the middle and relieving vomiting, warming the lungs and relieving cough, and detoxifying fish and crabs. China is the birthplace and one of the main producing countries of ginger, with a cultivation history of more than two thousand years. The traditional ginger production is to select strong, disease-free rhizomes in the harvest season and store them in a cellar. The next year, the germinated ginger is cut into pieces for planting. This method has serious waste and requires a lot of manpower and material reso...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008A01H4/005
Inventor 刘燃吴林王显凤李洪海刘静田庆忠姜玉松
Owner 重庆市幅沅农业生物技术研究院有限公司
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