Method for reproducing purple yam seedlings by tissue culture

A tissue culture, purple yam technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, to achieve the effects of high detoxification rate, simple detoxification recipes, and reduction of browning and enrichment

Inactive Publication Date: 2014-10-29
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no effective drug to prevent and cure the occurrence or spread of viral diseases, and modern biotechnology is used to select excellent disease-free seedlings for in vitro virus-free culture. Breeding non-toxic or less-toxic seedlings, replacing traditional vegetative propagation with tissue culture seedlings can fundamentally solve the problem of viral diseases

Method used

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specific Embodiment approach

[0015] (1) Germination of tubers: select a large tuber with no pests and diseases, few root hairs on the surface, and purple fleshy tubers after surface disinfection, and hide them in sand basins in the room (shed) for germination, and take explants after the shoots reach .

[0016] (2) Preparation of medium: including preparation of medium (initial medium, induction differentiation medium, strong seedling rooting medium, etc.), subpackaging, and disinfection.

[0017] (3) Disinfection and inoculation of explants: Cut off the shoot tip 1cm, remove the unfolded leaves and wash them with lotion. Sterilized with 70% alcohol on a sterile operating table, and then soaked with 0.1% Hgcl for disinfection and cleaning, then inoculated the shoot tip on the initial medium.

[0018] (4) Shoot tip culture: After the initial shoot tip culture survives, cut regenerated shoot shoot tips of 0.5-1 mm to inoculate to induce differentiation, proliferation and culture. When the multiplication co...

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Abstract

The invention provides a method for reproducing purple yam seedlings by tissue culture. The method takes a purple yam tender stem tip of 1.0cm as an explant and comprises the following steps: sterilizing the explant by 75% alcohol and a 0.1% mercuric chloride solution; inoculating the explant on an initial culture medium MS0; after the stem tip is survived, treating a regenerated tender stem tip of 0.5-1.0 mm; carrying out stem tip induction culture, stem tip subculture multiplication culture and rooting culture with different culture media at different periods to obtain a purple yam bottle seedling; after the seedling height is about 2cm, transplanting the bottle seedling into a nutrition bowl substrate for hardening; and culturing for 50-60 days till the seedling grows to be as high as 10cm, thereby obtaining the purple yam seedling. A stem tip induction multiplication culture medium is formed by MS, 0.5mg / L of 6BA and 0.1mg / L of NAA; a rooting culture medium is formed by 1 / 2MS, 0.2mg / L of NAA and 0.2g / L of Ac; each culture medium contains 45g / L of edible sugar and 6g / L of agar, and the pH of each culture medium is 5.7. According to the method, a germination accelerating manner is simple and convenient and the germination speed is high; a detoxification manner is simple and rapid and the detoxification rate is high; the variety is high in quality and pure; with the method, the industrialized production of purple yam tissue culture seedlings and the popularization of breed improvement can be realized.

Description

[0001] technical field [0002] The invention relates to a method for tissue culture and propagation of purple yam seedlings, which belongs to the technical field of plant asexual reproduction. Background technique [0003] Purple yam (Purple yam) is also known as yam or yam, long taro. Dioscoreaceae ( Dioscoreaceae ) Dioscorea ( Dioscorea ) is a local variety, which is a perennial vine, named after the tuberous or cylindrical epidermis and purplish red flesh. Purple yam is rich in viscous protein, purple anthocyanin, vitamins and choline, etc., and is rich in yam soap (natural DHEA), which contains various basic hormone substances. Regular consumption of purple yam can promote the synthesis of endocrine hormones and other unique dietary value, also known as "purple ginseng". It also has medicinal and nourishing functions such as strengthening the kidney, nourishing the lungs and benefiting essence, and softening blood vessels. Therefore, it is also known as the "K...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王碧琴余发新刘腾云肖亮
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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