25 results about "Common carp" patented technology

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The application discloses a method for screening superior parents of carp with high total content of polyunsaturated fatty acids. The method comprises obtaining training characteristic data and training target data, wherein the training characteristic data comprises SNP genotype information of training samples, and the training target data comprises total content information of polyunsaturated fatty acids of the training samples; training a ridge regression model by using the training characteristic data and the training target data to obtain a prediction model; obtaining SNP genotype information of a to-be-tested sample; obtaining a breeding value of the to-be-tested sample according to the SNP genotype information of the to-be-tested sample and the prediction model; and screening superior parents of carp with high total content of polyunsaturated fatty acids according to the breeding value. By the method, the breeding value of the to-be-tested sample can be directly obtained according to the SNP genotype information of the to-be-tested sample and the prediction model, and then the superior parents of carp with high content of polyunsaturated fatty acids can be screened from the to-be-tested sample, thereby providing excellent materials for new germplasm identification and breeding of carp.

The system according to this embodiment aims to provide rapid and consistent evaluation of bonsai and Nishikigoi (ornamental carp). [Solution] The system according to the embodiment comprises a collection unit, an analysis unit, and an evaluation unit. The collection unit collects images of bonsai trees and koi carp. The analysis unit analyzes the images collected by the collection unit. The evaluation unit calculates an estimated value based on the analysis results obtained by the analysis unit.

The invention relates to the technical field of carp culture, in particular to a culture method for carps in a high water temperature period and application of the culture method. According to the method, a breeding period is divided into an earlier stage, a high water temperature period and a later stage according to water temperature, and floating expanded compound feed, granular compound feed and high-energy floating expanded compound feed are fed respectively; the feeding rate, feeding times and dissolved oxygen of the three stages are controlled. The invention further provides application based on the breeding method. The breeding period is divided according to the water temperature, different feeds are fed in stages, and the feeding rate, the feeding frequency and the dissolved oxygen amount are controlled in stages, so that the damage and death conditions caused by ingestion of the carps in the high-water-temperature period are reduced; the carps can grow quickly in the early stage, liver and gall function health care is conducted, good ingestion and growth speed is kept in the high-water-temperature period, and high-speed growth and weight gaining are conducted in the later stage; the survival rate in the high-water-temperature period, the weight increment and the growth speed in the whole process are remarkably improved, and the problems that the growth performance and the survival rate of carps are low when the carps are cultured in the high-water-temperature period in the southwest region are solved.

The present application relates to the field of biotechnology, and more particularly to a method for knocking out runx2b gene of heterologous octoploid Changfeng crucian and application thereof. The present application designs gRNA-1 and gRNA-2 aiming at all the alleles of runx2b from two sets of parent sources of heterozygous gynogenetic crucian and Xingguo red common carp in Changfeng crucian; the mixture of two gRNAs synthesized by in vitro transcription and Cas9 protein is introduced into the animal pole of single-cell stage of Changfeng crucian fertilized eggs by microinjection, which can efficiently knock out all the 8 alleles of runx2b-A and runx2b-B. Any gRNA can guide the CRISPR / Cas9 system to simultaneously edit the 8 alleles; gRNA-1 achieves 97.5% of synchronous knockout rate, and gRNA-2 achieves 95% of synchronous knockout efficiency; when the double gRNA collaborative cutting strategy is used, the complete editing efficiency of the 8 functional copies reaches 100%.

The invention discloses a method for identifying germ cells of grass carps and carps by using dazl genes. The method comprises the following steps: cloning dazl cDNA sequences of the grass carps and the carps, and performing comparative analysis by using DNAMAN software; designing a universal non-specific primer, a grass carp specific primer and a carp specific primer according to sequence differences; extracting total RNA (Ribonucleic Acid) of testis of 12-month-old grass carp and carp, and reversely transcribing into cDNA Respectively carrying out PCR (Polymerase Chain Reaction) amplification by using three pairs of primers by taking the cDNA as a template; carrying out 1% agarose gel electrophoresis and imaging on the PCR product, and identifying and distinguishing germ cells according to the size of a strip; the method is simple in structure and reasonable in design, high-specificity primers are designed based on small difference of dazl gene sequences for the first time, precise amplification is achieved by combining with optimized annealing temperature (60 DEG C / 65 DEG C), special instruments are not needed, operation is easy and convenient, sensitivity is high, grass carp germ cells in carp gonads after transplantation can be rapidly identified, development of the'belly borrowing reproduction 'technology is supported, and the method is suitable for large-scale popularization and application. The grass carp breeding period is remarkably shortened, and the important industrialization prospect is achieved.

1. Name of the product in this design: Carp Model (3D Cloisonné Carp). 2. Purpose of this design: A creative carp model for display. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The invention relates to bacillus cereus KHCP-1, and the preservation number of the bacillus cereus KHCP-1 is CGMCC (China General Microbiological Culture Collection Center) No. 33399. The strain can efficiently hydrolyze fish scales of the cyprinus carpio within 24 hours under the activation conditions that the temperature is 40 DEG C, the pH value of a fish scale powder culture medium is 5, and 0.2 g / L of ZnSO4 is contained, the soluble protein content at the fermentation end is 1324.375 micrograms / mL, and the fish scale hydrolysis rate is up to 75% or above. The invention also relates to a new application of the fermented filtrate obtained by degrading the fish scales of the cyprinus carpio by the bacillus cereus KHCP-1 as a cosmetic raw material, and the additional value of the fish scales of the cyprinus carpio is improved.

The application discloses application of an Aeromonas vancleavei OmpW subunit vaccine as a vaccine for preventing bacterial septicemia of carps, and belongs to the technical field of genetic engineering and molecular immunology. The subunit vaccine protein is encoded by an Aeromonas vancleavei OmpW gene, the base sequence of the Aeromonas vancleavei OmpW gene is shown in the sequence table SEQ ID No. 1, the encoded protein is an Aeromonas vancleavei outer membrane protein OmpW subunit vaccine protein, and the amino acid sequence of the Aeromonas vancleavei outer membrane protein OmpW subunit vaccine protein is shown in the sequence table SEQ ID No. 4. After the subunit vaccine of the application is injected into carps, the fish body can be significantly stimulated to up-regulate expression of immune-related genes and produce a large amount of specific antibodies, the ability of the carps to resist Aeromonas vancleavei infection is effectively improved, and then bacterial septicemia of carps caused by Aeromonas vancleavei in carp culture is prevented.

The invention relates to the technical field of genetic breeding of aquatic animals, in particular to CBX7 protein related to high-temperature reversal of carps as well as a coding gene and application of the CBX7 protein. The invention identifies and analyzes the effect of the CBX7 protein in the carp high-temperature sex reversal, not only is beneficial to revealing the molecular mechanism of the carp high-temperature sex reversal, but also can provide a basis for establishing a sex detection marker and a directional breeding molecular tool, can realize sex detection of the carp sex reversal, can be applied to directional breeding for sex control, and has a wide application prospect. The method is especially suitable for breeding and popularization of all-female breeding.

The application discloses a SNP site for identifying high relative ovary weight carp parents and application thereof, and belongs to the technical field of molecular genetics and aquatic breeding. The application solves the technical problem that in the existing carp parent selection, there is no efficient molecular marker to accurately identify high relative ovary weight individuals. The application finds six SNP sites which are significantly related to the high relative ovary weight trait, and the six SNP sites are located at the 1914515th position of sequence NC_056581.1, the 17586713th position of sequence NC_056582.1, the 18667178th position of sequence NC_056592.1, the 23541881th position of sequence NC_056612.1, the 25898489th position of sequence NC_056597.1 and the 49224th position of sequence NW_024879353.1. The application realizes high-throughput molecular marker assisted selection of the carp parents, and improves the breeding efficiency and the breeding yield.

The invention discloses an aquatic feed for improving the bioavailability of L-ascorbic acid-2-phosphate and a preparation method of the aquatic feed. The feed comprises L-ascorbic acid-2-phosphate, phytase, a natural high-phytic-acid plant raw material (such as full-fat rice bran, and the phytic acid content is greater than or equal to 1.0%) and an organic acidifying agent (such as citric acid). By controlling the molar ratio of phytic acid to Vc ester to be greater than or equal to 3: 1 and adjusting the pH value of the mixture to 4.0-5.0, advanced hydrolysis of phytase to the Vc ester is inhibited by utilizing the competitive property of phytic acid in the feed puffing processing process, so that the retention rate of the Vc ester is remarkably increased (up to 88%); in in-vitro simulated digestion (a buffer system with the pH value of 5.8), after phytic acid is preferentially degraded, released phytase efficiently hydrolyzes Vc ester, and the Vc release rate reaches 72%. Through comprehensive calculation, the total effective Vc utilization rate reaches up to 63.4% and is increased by 48% compared with a control group. The feed does not need an embedding process, is low in cost, is simple and convenient to operate, and is suitable for aquatic feeds for carps, grass carps, tilapia mossambica, penaeus vannamei and the like.

This invention discloses gRNA and its application in constructing a new carp strain with high 1α,25-dihydroxyvitamin D3 levels. Belonging to the field of genetic engineering technology, it provides a gRNA, the nucleotide sequence of which is shown in SEQ ID NO.1. The invention also discloses the application of gRNA in constructing a new carp strain with high 1α,25-dihydroxyvitamin D3 levels, specifically in constructing a fast-growing and disease-resistant carp strain with high 1α,25-dihydroxyvitamin D3 levels. Specifically, this invention designs a highly specific target site for the carp cyp24a1 gene, synthesizes gRNA in vitro, and rapidly knocks out the cyp24a1 gene that degrades 1α,25(OH)2D3, resulting in a new carp strain with high 1α,25-dihydroxyvitamin D3 levels. 1α,25(OH)2D3 promotes fish growth and enhances fish immunity, making this fish strain of significant application value.

The application belongs to the technical field of aquatic genetic breeding, and particularly relates to a breeding method of a few intermuscular bones strain of a carp based on whole genome selection. The reference population is established by determining the number of intermuscular bones of the carp, the candidate population is established, then the reference population and the candidate population are resequenced, and high-quality SNP typing results are obtained; the genetic parameters of the target traits are estimated according to the high-quality SNP typing results, the genomic breeding value (GEBV) of the candidate population is further estimated, the candidate individuals are selected as the parent fish according to the GEBV value, and the breeding of the few-spine F1 generation seedlings is carried out. The purpose of breeding the few-spine fine strain is achieved by only calculating the GEBV value through gene sequencing without killing the fish. The application develops a set of genome selection scheme suitable for the improvement of the number of intermuscular bones of the carp, the operation is clear, and a new molecular marker assisted breeding technology is provided for the breeding of the few-spine fine strain of the carp.

The embodiment of the utility model provides a carp type power transmission landscape tower. The carp type power transmission landscape tower comprises tower legs, a tower body and a tower head. In the tower head part, the left ground wire cross arm and the right ground wire cross arm are set to be'fish beards', the left wire cross arm and the right wire cross arm are set to be'fins', and decorating parts are arranged on the tower head to increase the characteristics of'fish eyes' and'gill ', so that the arrangement of'fish head' and'fish body 'of the'carp' is completed in the tower head part. The tower body and the tower legs are shortened, so that the appearance of the tower body and the tower legs is close to the fish tail of a carp. All parts of the iron tower are converted into carp landmark parts, so that the iron tower has a better carp shape, the environment can be beautified, and meanwhile the structure further has good bearing capacity and stability.

The invention discloses application of carp roe lectin rCcFEL2 in preparation of a carp culture feed additive. The carp roe lectin rCcFEL2 has dual effects in improving disease resistance and growth performance of carps. The antibacterial function and the growth regulation effect of the carp roe lectin rCcFEL2 are systematically clarified for the first time, and the research blank of the lectin in antibacterial and immunological enhancement application of aquatic products is filled. An in-vitro antibacterial experiment proves that the carp roe lectin rCcFEL2 shows remarkable antibacterial activity on various common gram-negative bacteria and gram-positive bacteria in aquatic products, and is wide in antibacterial spectrum and stable in action effect; in-vivo challenge tests further show that the carp roe lectin rCcFEL2 can significantly enhance the disease-resistant defense capability of carp bodies and ovaries, effectively reduce the bacterial load in immune tissues infected by pathogenic bacteria, actively regulate the growth performance of carps and promote healthy growth of the bodies.

The utility model relates to adjustable slip-joint pliers which comprise a pliers body, a shaft adjusting assembly and two connecting assemblies. The clamp body comprises two long arms, two handles and two clamp heads, and the two ends of each long arm are connected with one handle and one clamp head respectively; the shaft adjusting assembly comprises sliding grooves formed in the inner sides of the two long arms, each sliding groove is internally provided with a sliding block, each sliding block can slide in the corresponding sliding groove, a rotating shaft is connected between the two sliding blocks, the two ends of the rotating shaft are elastically connected with clamping blocks, and the clamping blocks are arranged in the sliding grooves. A plurality of clamping grooves matched with the clamping blocks are formed in the outer side walls of the two long arms; the slip-joint pliers can meet the requirements of different forces, the working efficiency is improved, the operation flexibility is enhanced, workpieces and tools are protected, the plier heads of different types can be conveniently replaced by the slip-joint pliers, then different working requirements are met, and the utilization rate of the tools is improved.

The application discloses a weak strain of Cyprinid herpesvirus 2 (CyHV-2) G-RP7 for preparing a common carp hematopoietic organ necrosis disease vaccine, and the preservation number is CGMCC No. 24099. The weak strain is used to infect a goldfish fin cell line (RyuF-2), and after 4-5 days, typical cytopathic effect (CPE) is observed on the cells, and the continuous passage can stably cause the cytopathic effect. The weak strain is sensitive to the RyuF-2 cell line, realizes in-vitro stable culture and amplification of the weak strain, and the TCID50 of the virus suspension is determined by a Reed-Muench method 50 up to 10 5.9 / ml. The virulence of the weak strain does not return to be strong after continuous in-vitro and in-vivo passages. The weak strain can be used for preparing the common carp hematopoietic organ necrosis disease vaccine, and both immersion immunization and intraperitoneal injection immunization can stimulate the fish to produce specific immune response, and have good anti-CyHV-2 infection effect.

The invention belongs to the technical field of molecular biology and microbiology, and particularly relates to an antibacterial peptide for resisting spring viremia of carp virus and application thereof, and the antibacterial peptide is any one of the following: 1) an amino acid sequence as shown in SEQ ID NO.1; and 2) a nucleotide sequence for coding the amino acid sequence as shown in SEQ ID NO. 1. The invention reveals that the antibacterial peptide NKLj27 has remarkable anti-SVCV activity for the first time, can effectively inhibit infection of SVCV on EPC cells at the cellular level, and alleviates cytopathy and plaque formation caused by viruses. Meanwhile, the NKLj27 shows a good protection effect on SVCV infection at a carp living body level, the death rate caused by virus infection can be remarkably reduced, and the evidences show that the NKLj27 has remarkable anti-SVCV activity. The invention provides a new candidate drug for prevention and control of fish virus diseases.

The invention provides a mixed fry breeding method for a land-based round pond and a rice field, and belongs to the technical field of aquaculture. According to the method, the land-based round pond and the rice field are mixed to cultivate the carp fries, the survival rate of the fries is increased, grading is easy, the cultivation cost is reduced, pollution caused by discharge of cultivation tail water is reduced, the small water body is effectively utilized to complete batch production of the carp fries, the land resource utilization rate is increased, income increase of farmers is promoted, and a solid guarantee is provided for village revitalization.

The utility model provides a carp feed storage device which comprises a base, an installation cylinder is fixedly installed on the top of the base, a carp feed containing cylinder is arranged in the installation cylinder, a quantitative feeding mechanism is arranged at the bottom of the carp feed containing cylinder, and the bottom of the quantitative feeding mechanism penetrates through the installation cylinder and extends to the position below the installation cylinder. A containing vessel is placed on the top of the base and located below the quantitative feeding mechanism. The base is arranged for mounting and supporting the mounting cylinder, the mounting cylinder is arranged for accommodating the carp feed containing cylinder, and the carp feed containing cylinder is arranged for storing carp feed; the quantitative feeding mechanism is arranged at the bottom of the carp feed containing barrel and used for achieving quantitative discharging of feed stored in the carp feed containing barrel, fancy carp feeding is facilitated, and meanwhile the situation that when a traditional packaging bag is frequently opened during taking, internal feed is affected with damp, and subsequent fancy carp eating is affected is avoided.

The utility model provides a fish braising vehicle, and belongs to the technical field of snack vehicles. The fish braising vehicle comprises a vehicle body, a gas stove, a gas connector, a fish braising basin, a filter plate and an extending storage mechanism, the gas stove is installed in the vehicle body, the gas connector is arranged on the gas stove, the fish braising basin is inserted in the vehicle body, the fish braising basin is also erected on the gas connector, and the filter plate is arranged on the extending storage mechanism. The filter plate is inserted into the braised fish basin, the extending storage mechanism is installed on the outer wall of the vehicle body and used for storing more objects, the self-locking universal wheels are fixed to the lower surface of the vehicle body, and the self-locking universal wheels are arranged on the outer wall of the vehicle body. According to the fish braising vehicle, the third handle, the fourth handle and the plate body are utilized, so that the number of the Yellow River large carps can be visually observed, selling of the Yellow River large carps is not affected, the completeness of the carps can be ensured, and better use experience is brought to a user.

The application provides a composition with a killing effect on golden apple snails and application thereof, and belongs to the technical field of pest control. Effective active ingredients of the composition include discarded tobacco leaf extract and orange peel essential oil. The composition is extracted from natural plants, has high efficiency and safety, can effectively achieve the prevention and control of harmful soft-bodied animals, i.e., golden apple snails, has a significant killing effect on the golden apple snails at a low concentration, and is verified by experiments to have no toxicity to 3-4 cm carp fry at an effective concentration, fully indicating that the composition is safe to non-target organisms and has low environmental risk.

The invention relates to the technical field of biology, and particularly discloses a carp temperature-induced reverse character breeding method based on genome selection. The method comprises the following steps: (1) determining a sex reversal phenotype value of each individual in a reference group; (2) obtaining a plurality of SNP markers according to the whole genome sequencing data of each individual; (3) constructing a genome relation matrix; (4) predicting a genetic breeding value by using a linear hybrid model, and selecting and remaining individuals with high breeding values as candidate parents; the linear mixed model is y = 1 mu + Zg + e; y is a sex reversal phenotype value, mu is a sex reversal phenotype value mean value of each individual in the reference group, Z is an incidence matrix of y and g, g is an additive genetic effect GEBV, and e is a residual error. The method provided by the invention can realize the selection of the temperature-induced reversion character of the carp in the early stage, improve the breeding efficiency and remarkably shorten the breeding period.

The present invention relates to a method for high-density milkfish larval rearing comprising the steps of cleaning and chlorinating a culture tanks overnight; rinsing culture tanks and installing aeration lines and air stones; stocking a dechlorinated seawater; adding green algae (Nannochloropsis sp.) at a density of 1 X 106 cells / ml; stocking a milkfish larvae at stocking density of 60 to 120 milkfish larvae per liter; feeding the stocked milkfish larvae with rotifer, Artemia, and artificial diet; adding probiotics; partially changing the dechlorinated seawater daily; and employing flow-through system to the culture tanks between 6 PM to 8 AM starting on day 9 up to the harvest period of the milkfish larvae. By increasing larval stocking density, production capacity of the hatchery is increased, and disposal of milkfish eggs is minimized. The disclosed method improves the production cost and returns in milkfish hatchery operations without affecting the fry quality.