198 results about "Common carp" patented technology

The invention relates to a mixed feed for mixed aquaculture of freshwater fishes. The mixed feed includes a basic material. The basic material includes fish meal, soybean meal, wheat, cottonseed meal, rapeseed meal and soybean oil, and also includes by weight: 1.5-2.2% of calcium phosphate, 1.5-2.2% of bentonite, 1.5-2.2% of zeolite powder, 0.1-0.25% of choline chloride, 0.1-0.25% of salt, 0.02-0.05% of compound probiotics, 0.04-0.08% of vitamin C, 0.005-0.02% of allicin, 1.0-2.0% of compound vitamins and 1.0-2.5% of compound mineral salts. The feed is suitable for intensive culture or polyculture of megalobrama amblycephala, black carp, grass carp, common carp and the like, so that the freshwater fish feed is generalized, the return rate of the feed is improved, the aquaculture water eutrophication and fish liver and gallbladder diseases are reduced, the output of unit aquatic products is raised, and the economic benefits of farmers are significantly improved.

The invention discloses giant salamander compound bait and a production method thereof. The giant salamander compound bait includes bait for young salamanders and adult salamanders and bait for parent salamanders. The salamander compound bait takes fresh crucian carps, carps, grass carps, loaches and bull frogs as main materials and takes aggregates, mineral powder, micro amino acid and the like as auxiliary materials. After the main materials are mixed and minced, the auxiliary materials are added, and starch is then mixed. After being stirred and rubbed, the materials are filled by plastic casings in a sausage stuffer and dotted. Finally, sausages are sterilized at a temperature of 95-100 DEG C. The giant salamander compound bait can replace fresh fish and shrimp bait to feed giant salamanders. When the giant salamander compound bait is used for feeding, the plastic casings are stripped off, and the giant salamander compound bait is cut into strip materials suitable for the giant salamanders to swallow and thrown into a salamander pond. The giant salamander compound bait has good palatability. Practices prove that when the bait is used for feeding the giant salamanders, the breeding cost of the giant salamanders can be reduced by 30-50%, the growth rate of commercial salamanders can be increased by 10-30%, the salamander reproduction rate can be increased by 50-100%, and thedisease incidence rate of the giant salamanders is obviously reduced.

The present invention discloses one kind of carp feed, which consists of soybean dregs 35-50 wt%, cotton seed dregs 15-25 wt%, DDGS 3-8 wt%, fish meal 3-8 wt%, starch 6.2-16.1 wt%, corn dregs 8-12 wt%, calcium dihydrogen phosphate 1.5-1.7 wt%, salt 0.2-0.4 wt%, Chinese medicinal stone 1.5-2.5 wt%, soybean oil 0.5-1.5 wt%, and premixed fish feed material 0.8-1.2 wt%. It has comprehensive balanced nutrients, and can promote the growth of carp and raise its disease resistance.

The invention discloses a planting and breeding integrated ecological breeding method for a large fishpond. Famous and special breeding varieties such as common carps, golden carps, grass carps, chubs, penaeus vannawei, loaches, soft-shelled turtles, river crabs and the like are put in a suitable place to breed in a breeding pond; and the stocking ratio of herbivorous fishes to forage fishes to demersal residual feed animals by weight percentage is (35-40%) to (50-55%) to (10-15%). Proper biological varieties are selected according to biological requirements of ecological niches and cooperate to build an eco-friendly environment, forage grasses are planted for feeding the herbivorous fishes, the feces of the herbivorous fishes and a water body can produce primary biological baits, the primary biological baits are supplied to shrimps and secondary nutritional fishes, and organic detritus produced by the water body sinks to the bottom of water and is ingested by the demersal loaches, crabs and the like. At the same time, lotus roots and cane shoots are planted in the breeding water body and nutritive salt in the water body is removed for purifying the water, so that a novel high-efficiency energy-saving planting and breeding integrated ecological breeding mode is formed.

The invention relates to a method for preparing three-classification whole blood control substance simulacrum and a blood type detection reagent box made by the method. The method comprises the preparation of red blood cell simulacrum, white blood cell simulacrum and blood platelet simulacrum. The concentration of the prepared red blood cell simulacrum, white blood cell simulacrum and blood platelet simulacrum is adjusted to ensure that cell particle is suspended in cell suspension and is used as monomial substance control substance, thereby forming whole blood control substance. The invention aims to solve the source of various cells and provides a method for preparing three-classification whole blood control substance. The invention adopts fish blood to simulate human white blood cell and blood platelet, and waste red blood cell of a livestock red blood cell plasma-mixing station to simulate human red blood cell, thereby simulating the practical histogram distribution of human blood sample; moreover, the cell suspension adopting the formula of the invention has longer storage life; in addition, the method can carry out simulation operation when adopting the red blood cells of silver carp, grass carp, common carp and crocodile, etc. during experiment, and can obtain excellent quality-control effect through fixative treatment.

The invention relates to a process for preparing a cyprinus carpio raw fish ball product. The process comprises the following specific steps of: preparing raw material fish, performing pretreatment, flesh separating, rinsing, dehydrating, mincing, performing refined filtration, dehydrating, blending, molding, setting, cooling, packaging, IQF freezing and storing. The preparation method for processing cyprinus carpio raw fish balls provided by the invention is low in investment cost, is feasible, and is used for improving the additional value of cyprinus carpio deep processing, and then comprehensive utilization of cyprinus carpio is realized.

The invention discloses a method of using carp skin for the enzymatic hydrolysis to prepare collagen, comprising the raw material treatment, non-collagen protein removing by sodium chloride, fat removing by petroleum ether extraction, collagen and papain extracting by citric acid, flavor enzyme common hydrolysis, vacuum concentration and spray drying. Then, carp skin collagen peptide which is white and has no fishy smell with the relative molecular weight of 8,000-15,000 is obtained. The enzyme hydrolysis process of the invention adopts the papain and the flavor protease to simultaneously hydrolyze the carp skin collagen, the enzyme hydrolysis time is shortened greatly which only requires 30min. A petroleum ether extraction method is used for removing the fat. Therefore, fats and residues of fat-soluble pigment in finished product of the carp skin collagen peptide are reduced greatly. The quality of the finished product is improved. The method has the advantages of high yield and collagen peptide extraction rate and perfect product purity.

The invention relates to a feed additive of aquaculture industry, in particular to a meat quality flavor modifying agent for grass carp or carp and a preparation method thereof. Each kilogram of modifying agent comprises the following components: 30 to 50g of seaweed extract, 10 to 20g of combination of inosinic acid and guanylic acid, 20 to 30g of carnitine, 15 to 30g of glycin, 10 to 20g of alanine and the balance of zeolite powder. The preparation method comprises the following steps: firstly, crushing solid materials in the seaweed extract, the combination of inosinic acid and guanylic acid, the carnitine, the glycin, the alanine and the zeolite powder materials and sieving the crushed solid materials through a sieve with 80 to 100 meshes for later use; and secondly, mixing and stirring the crushed solid materials in the proportion to ensure that the coefficient of variation CV of the uniformity is less than 5 percent to obtain the product. The modifying agent can effectively improve protein content, content of total amino acids, content of essential amino acids and content of delicious amino acid in muscles of the grass carp and the carp.

The invention relates to an ecological health-care type special feed additive for carps. The ecological health-care type special feed additive is composed of the following components in parts by weight: 10-15 parts of pine needle meal, 2-6 parts of albizia flower, 3-6 parts of poria cocos, 2-4 parts of white paeony roots, 3-5 parts of schizonepeta, 2-4 parts of honeysuckle stems, 3-6 parts of cordate houttuynia, 2-4 parts of semen cuscutae, 3-4 parts of cortex acanthopanacis, 3-5 parts of zeolite powder, 2-4 parts of carrots, 1-1.5 parts of probiotics powder, 0.2-0.5 part of chitosan and 0.2-0.4 part of allicin. The ecological health-care type special feed additive for the carps disclosed by the invention has the advantages that the dose is small, only 0.2-1% of the additive needs to be added into a compound feed and the adding cost is low; the economic benefits of culture of carps can be obviously improved and diseases in the cultivating process of the carps can be obviously lowered; the survival rate is improved by 10-12%; the feed conversion rate in the cultivating process of the carps can be effectively improved; and the growth of the carps is promoted and the quality of fish is improved.

The invention discloses a pond fish culturing technology. A method includes the following steps that a pond is cleaned, water is discharged, after the pond is cleaned, the pond is exposed to the sun till the bottom of the pond cracks, quicklime is used for disinfecting the bottom of the pond, the water discharging depth is 1.3 m, high-quality fry are selected for well completing the inspection and quarantine work in the fry throwing process, according to the throwing density and proportion of the fry, 400 grass carps are thrown per mu, each grass carp weighs 50-150 g, 10 silver carps are thrown per mu, each silver carp weighs 50-250 g, 300 golden carps are thrown per mu, each golden carp weighs 50-100 g, 100 common carps are thrown per mu, and each common carp weighs 50-100 g, water quality and water level control is conducted in daily management, fodder is used for feeding, taking the grass carps and the silver carps as an example, ryegrass is taken as the main fodder for the grass carps and the silver carps, disease prevention is conducted, powder chlorine dioxide is used for disinfecting and sterilizing, three times of insect killing is appropriate all the year round, trichlorfon and copper sulfate are generally used for insect killing, and catching and stocking are conducted in rotation. According to the method, the yield and the quality of polyculture fishes can be effectively improved, the diseases are reduced, and the culture benefits are improved.

The invention discloses a culturing method for sex reversal of a female carp to a male carp. The culturing method comprises the following steps that A. squeezing an abdomen of a 10-11-month-old carp, and separating a male carp with out-flown semen and a carp with no out-flown semen; B. inserting a copper roe digging device in a genital pore of the carp from which semen can not be squeezed, and selecting the carp capable of obtaining roe particles to cultivate the carp in a cement pond; C. according to the weight of the carp, feeding the carp with dosing fodders containing 17 alpha- methyltestosterone every day, and mix-injecting domperidone, luteinizing hormone releasing hormone and carp pituitary gland in the thoracic cavity of the carp in each five days; and D. after continuously processing the test carp for 2 months, squeezing the abdomen of the carp, the test carp with out-flown milky white semen is the sex reversal carp which is a female on genotype and a male on physiology. The culturing method is easy and feasible and is convenient in operation, and can be used for sex reversing the female carp on genotype into a male carp on physiology. The growth speed of the filial generation cross bred by the sex reversal carp and the common carp is fast and the economic benefits are remarkable.

The invention discloses a fish cake formula and a manufacturing method and superinduces a new menu of Chinese food culture and leads to fresh, savoury, tender, soft and elastic taste when people eat. Technical problems to be solved lie in: firstly, the formula for manufacturing fish cake comprises 20 percent of fish meat which is heaved into paste, 15 percent of starch, 5 percent of white sesame powder, 10 percent of ripe peanut flour, 10 percent of fermented cake head, 40 percent of clean edible water and a little ginger, onion and monosodium glutamate; secondly, the fish cake prepared through the fish cake manufacturing method is transparent, soft, savoury, tender and elastic and can be both eaten directly and used for chaffy dish. With abundant sources, the fish cake formula and the manufacturing method is suitable for fleshy fishes of lotus fish, black carp, common carp, etc.

The invention relates to an artificial eel propagation method, which includes: a, ripening parent fishes, breeding catadromous parent reels for seven days, gradually increasing salinity of water, and subjecting the parent eels to back intramuscular injection of ripening agent-carp pituitary gland homogenate and HCG (human chorionic gonadotropin); b, performing induced spawning of the parent eels, subjecting female eels to injection of oxytocin 17 alpha-hydroxyl progesterone and HCG, subjecting sperm-producing male eels to injection of mixture of CPE (carp pituitary extract) with HCG, collecting naturally spawned fertilized eggs on the next day, and subjecting the female eels laying no eggs to semi-dry artificial insemination; and c, hatching the fertilized eggs, immediately transferring the fertilized eggs to a special-made hatching barrel for hatching, and slightly aerating, changing half of water every four hours until fry are hatched successfully. The artificial eel propagation method has the advantages that anesthetic loss and operational injury can be prevented, injection dosage of hormones is reduced, a great amount of eels can mate and lay eggs naturally under artificial induced spawning, artificial insemination can be complete securely in a planned way, level of high-quality fertilized eggs and alevin of eels artificially propagated is increased.

The invention provides a bait formulation specially used for fishing carps, consisting of wheat bran, rapeseed cake, rice, millet, shrimp powder, rice wine, sugar, perfume, peanut cake and mature corn powder; the wheat bran, the rapeseed cake, the rice, the millet and the peanut cake are mixed and crushed as powder where water is added; subsequently, the mixture is cooked as paste-shaped material in which shrimp powder, rice wine, sugar, perfume and mature corn powder are mixed, thus gaining the bait. Carps like fragrance and sweet taste and like acid taste secondly; the bait formulation aims at the characteristic of the carps, adopts the raw materials with fragrant and sweet taste for the formulation, and has low cost and good effects.

The invention discloses a protein feed raw material prepared from Asian carps and is used for animal breeding and a preparation method for the protein feed raw material. The preparation method comprises the steps of performing smashing pretreatment on the Asian carps, mixing the smashed Asian carps with a microorganism preparation, and then performing fermentation so as to obtain the high-quality protein feed raw material with balanced protein and amino acid. The protein feed raw material can be widely applied to livestock breeding and animal aquaculture. Strains of a microorganism leavening agent comprise at least three of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, lactobacillus acidophilus and pediococcus pentosaceus. A method which is low in energy consumption and low in cost and does not have any waste emission is supplied to deep processing of the Asian carps, so that the ecological problem caused by the Asian carps in America can be effectively controlled, and protein resources of the Asian carps are fully used; the prepared protein feed raw material can guarantee no loss of nutrition of the raw material, and polypeptides, cellulose, oligopeptide, oligopeptide mixtures, trace elements and the like can be generated; the optimal nutrition is supplied to animal breeding.

The invention provides an artificial breeding method for glyptosternon maculatum. The artificial breeding method includes the following steps that A, after wild glyptosternon maculatum parent fishes are immersed in a saline solution, sex discrimination is conducted, and female fishes and male fishes are collected; B, a medicine composition for female fish spawning induction is prepared from 5 micrograms of lutropin releasing hormones and one carp hypophysis per kg of the female fishes, 24 hours after injection, the abdomens of the female fishes are slightly pressed, and if no free egg granule flows out, a second shot of spawning induction medicine of the same dosage is injected; C, dry method fertilization is adopted; D, after egg membranes of zygotes disengage, the zygotes are poured into an incubation tank to be incubated, when glyptosternon maculatum fry begin to swim at the bottom of a pool, exogenous nutrition is turned to, and animal live bait and artificial compound feed are fed. The nature rule of the wild glyptosternon maculatum parent fishes is grasped, the artificial propagation technology of glyptosternon maculatum is studied, a set of feasible artificial spawning induction and artificial incubation method is formed, and artificial large-scale breeding of glyptosternon maculatum is achieved.

Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

The invention discloses a compound feed for wild carps. Breeding experiments indicate that the compound feed for wild carps solves the problem that the artificially cultured wild carps are disadvantageous to natural wild products in terms of color, flavor and figure, namely the artificially cultured wild carps have dark body color, fat body figure and unnatural flavor, and that the compound feed for wild carps can retain the body figure and meat quality (property) of natural wild carps and promote the growth of the carps.

The invention relates to a Chinese herbal micro-ecologic farmed fish feed suitable for carps in a medium period and a preparation method thereof. The Chinese herbal micro-ecologic farmed fish feed is prepared from the following raw materials: wheat middling, corn germ meal, rice bran, wheat, bean pulp, extruded soybean, rapeseed meal, cottonseed meal, distillers dried grains with soluble (DDGS), fish meal, spray-dried animal blood cells, soybean oil, liquid phospholipid, monocalcium phosphate, 50 percent of choline chloride, 0.04-0.08 percent of 35% Vc phosphate, glossy privet fruit, special compound enzyme for aquatic products, microecologics for aquatic products, and 1.2 percent of a special premix for carps. The Chinese herbal micro-ecologic farmed fish feed is high in pertinence, is especially suitable for breeding and feeding of carps in a medium period, is balanced and complete in nutrient and good in luring property, and has the advantages of enhancing the immunity, the disease resistance and the stress resistance of carps, fully ensuring nutrients necessary for growth and development of carps, achieving the effect of rapid and healthy growth, increasing the feed conversion ratio, alleviating environmental pollution, improving water quality, and maximally protecting nutrient substances from being damaged by high temperature in a preparation process.

The invention relates to a method for producing color carp with 'white-and-red' body color. The method comprises the steps that: (1) hybrid parental Oujiang color common carps, the body color of which are 'full red' and 'pink jade', are selected as male parent and female parent; (2) matching and reproduction; (3) fry rearing; (4) body color selection: Oujiang color common carps which bodies appear interlaced red and white patches are selected, that is the individual carps with 'white-and-red' body color are selected for separate breeding. Oujiang color common carps with 'white-and-red' body color produced by the method of the invention can be used as a novel ornamental fish, and increase the types of body colors of Oujiang color common carps. Meanwhile, the method also lays a solid foundation for breeding improved variety of Oujiang color common carps and developing body color of Oujiang color common carps.

The invention belongs to the technical field of agricultural ecology and particularly relates to a method for raising fishes in a paddy field. The method comprises the following steps: A, selecting a piece of paddy field: selecting the field with clean water, sufficient water source, convenient irrigation and drainage, good water and fertility retention performance and no drought and flood influence; B, rearranging the piece of paddy field: digging a fish ditch with the depth of 50-80cm and the width of 50-100cm; digging a fish pit with the depth of 1-1.5m and the area accounting for 5-8% of that of the paddy field; connecting the fish ditch with the fish pit; heightening and reinforcing a ridge; forming a water inlet and a water outlet in the corresponding corners of the paddy field; C, raising the fishes: starting to throw fingerlings after rice seedlings turn green 7-15 days after seedling transplantation, wherein the fingerlings are thrown according to a quantity ratio of grass carp to common carp to golden carp of 1 to 1 to 1 and 10-20kg of the fingerlings are thrown per mu; and D, performing raising management. The method is easy to implement and high in fish yield per mu.

The invention discloses a recombinant carp Nrf2 (NF-E2-related factor 2) gene, a recombinant carp Nrf2 protein, preparation and detection methods and application of the recombinant carp Nrf2 gene. The recombinant carp Nrf2 gene has a sequence shown by SEQIDNO:1, and the recombinant carp Nrf2 protein has a sequence shown by the SEQIDNO:2. By the nucleotide sequence of the carp Nrf2 gene complementary deoxyribonucleic acid (cDNA) disclosed by the invention, the tissue specific expression of the Nrf2 gene can be further detected through a reverse transcription-polymerase chain reaction (RT-PCR) or hybridization method, and the full-length cDNA of the carp Nrf2 gene can be further cloned. The complete Nrf2 protein may be expressed through recombining the cDNA of the recombinant carp Nrf2 gene onto an expression vector, so that the Nrf2 protein can be used for producing antibodies, and the Nrf2 gene expression states of cells, tissues, early embryos and individuals of carps under different states are detected.

The invention provides a compound feed for carp, and the compound feed can be used for solving the problems that the existing compound feed for the carp can not meet the demands of environmental and social development and is poor in feeding effect. The technical scheme is as follows: in the compound feed for the carp, one or more of a microecological preparation, a plant extract and yeast cell wall polysaccharides are added. The compound feed for the carp, provided by the invention, is scientific in mixture ratio, economical, and simple and easy in operation method, and can be used for promoting the growth of the carp, improving the immunity of the carp and creating good economic value.

The invention provides an ecological fish-farming method. The ecological fish-farming method is characterized in that water treatment is carried out by using Chinese herbal medicines so as to prevent fish diseases. In a typical embodiment of the invention, a Chinese herbal medicine composition comprising thymus quinquecostatus, radix glycyrrhizae, radix bupleuri, radix scutellariae, roots of Chinese pulsatilla, rhizoma cimicifugae and garlic is added into a fishpond for farming gold-wing carps with good effect. In a preferable implementation way, the fishpond is kept to be communicated with an external outer source, so that water is changed with small flow per day; water diversion quantity is 1 / 32 to 1 / 4 the volume of fishpond water; and flow regulation is carried out according to different seasons. The fish-farming method disclosed by the invention can keep good water quality, and is beneficial for healthy growth of freshwater fish; chemicals are not required, so that green ecological farming is realized, and therefore, common carp fry can be farmed into gold-wing carps.

The invention discloses a monoclonal antibody for resisting to cyprinus carpio haematopterus IgM, a hybridoma cell strain and application of the monoclonal antibody. The monoclonal antibody for resisting to cyprinus carpio haematopterus IgM is secreted and produced by the hybridoma cell strain A5-E10 with the preservation number being CCTCC NO: C2015218. The monoclonal antibody can be applied to detection of certain pathogeny of cyprinus carpio haematopterus or common carp or a vaccine specific antibody of an aquatic product, thereby early-stage disease diagnosis and vaccine using effect evaluation are conducted, high clinical application and promotion value is achieved for epidemiological investigation and pathogeny diagnosis conducted on disease of cyprinus carpio haematopterus or common carp, and reference is provided for prevention and treatment of other cultured fish diseases.

The invention relates to a carp and chub cultivation method, and belongs to the field of aquatic product cultivation. The carp and chub cultivation method comprises the following steps: conducting pond washing disinfection treatment on a fishpond before fries are bred, injecting water in the fishpond, using quick lime for adjusting and controlling the pH value of water quality to be in the range of 7-7.5, breeding the fries after the fishpond is filled with water, breeding white shrimps in the fishpond after the fries are bred by two weeks, enabling fish foods to be put two days once, enabling buoyant pellet feed to serve as the fish food, wherein the protein content in the buoyant pellet feed is 25%-30%; supplementing fresh water in the fishpond regularly to guarantee the valid water level of the fishpond, wherein the supplemented fresh water shall be aired for 5-7 days; putting aquatic plants into the fishpond at 1-2 month interval, and enabling the putting quantity of the aquatic plants to be 5-15kg per mu. The aquatic plants are put into the fishpond, therefore, cultivated fishes are closer to wild fishes, the meat quality of the fishes can be improved, the physical conditions of people eating the fish are improved in a converted mode, and meanwhile the ecological environment in the fishpond is protected.

The invention discloses a method for promoting natural egg reproduction of common carp and crucian carp. The method comprises the steps of making spawning nests with a natural herbaceous plant material for eggs of common carp and crucian carp to be adhered to; placing the spawning nests into a water area at the proper time; after the spawning nests are placed well, installing underwater lights in water below the spawning nests at intervals of 50 meters, wherein the underwater lights are used for inducing common carp and crucian carp to enter the spawning nests to lay eggs for reproduction; and every 3-5 days, spraying a proper amount of salt solution or potassium permanganate solution onto the surfaces of the spawning nests, so that the eggs can be adhered more firmly and embryonic development of healthy fertilized eggs can be protected. With the adoption of the method, common carp and crucian carp resources in lakes and reservoirs can be proliferated effectively. The spawning nests and a corresponding technique provided by the invention are simple in technology and low in cost, and no advanced facility instruments are required.

The invention relates to a FFRC strain common carp mature ovarian tissue frozen section making method and belongs to the technical field of tissue sections. Mature ovarian tissues are taken out from a FFRC strain common carp, and a FFRC strain common carp mature ovarian tissue frozen section is obtained after fixation, degreasing, dehydration, embedding, freezing and slicing are performed. Compared with a common general freezing and slicing method, degreasing is performed through pure acetone, excessive fat in a later development period of FFRC strain common carp mature ovaries can be efficiently removed, and accordingly the frozen ovarian section high in quality and clearer in structure is obtained. An important and reliable molecular experiment material is provided for tissue localization and functional study such as immunohistochemistry of FFRC strain common carp mature ovarian genes, and the FFRC strain common carp mature ovarian tissue frozen section making method has the important significance on molecular mechanism study of good economic characters of FFRC strain common carps.

The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.