49 results about "Common carp" patented technology

The invention discloses a method for screening carp dominant parents with high total polyunsaturated fatty acid content. The method comprises the steps that training feature data and training target data are obtained, the training feature data comprise SNP genotype information of a training sample, and the training target data comprise polyunsaturated fatty acid total content information of the training sample; training the ridge regression model by using the training feature data and the training target data to obtain a prediction model; obtaining SNP genotype information of a to-be-detected sample; obtaining a breeding value of the to-be-detected sample according to the SNP genotype information of the to-be-detected sample and the prediction model; and screening the carp dominant parents with the total content of polyunsaturated fatty acids according to the breeding values. According to the method, the breeding value of the carp can be directly obtained according to the SNP genotype information of the sample to be detected and the prediction model, so that dominant parents with high carp polyunsaturated fatty acid content are screened from the sample to be detected, and an excellent material is provided for new carp germplasm identification and breeding.

The invention provides a cyprinus carpio sex determining gene ccMD and application thereof in cyprinus carpio sex determination or sex control, the full length of the cyprinus carpio sex determining gene ccMD is as shown in SEQ ID NO.1, the CDS sequence is as shown in SEQ ID NO.2, and the protein coded by the gene is as shown in SEQ ID NO.3. The cyprinus carpio sex determining gene ccMD can be applied to cyprinus carpio sex determination or sex control. Specific primers are designed according to the gene sequence characteristics, and XX, XY and YY carps can be rapidly and accurately identified. The gene is taken as a target gene, a gene editing technology and a gene transfer technology are adopted, hormone treatment is not needed, and a genetically male carp and a physiologically female carp or a genetically female carp and a physiologically male carp can be rapidly obtained.

Provided are a Cyprinus carpio sex-determining gene ccMD and a use thereof in Cyprinus carpio sex identification or sex control. The Cyprinus carpio sex-determining gene ccMD has a full-length sequence as shown in SEQ ID NO: 1 and a CDS sequence as shown in SEQ ID NO: 2, and a protein coded by the gene is as shown in SEQ ID NO: 3. Cyprinus carpio XX, XY and YY can be quickly and accurately identified by designing a specific primer using the gene sequence characteristics. Genetically male but physiologically female Cyprinus carpio or genetically female but physiologically male Cyprinus carpio can be rapidly obtained by taking the gene as a target gene, and using the gene editing technology and the gene transfer technology without hormone treatment.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The application discloses a method for screening superior parents of carp with high total content of polyunsaturated fatty acids. The method comprises obtaining training characteristic data and training target data, wherein the training characteristic data comprises SNP genotype information of training samples, and the training target data comprises total content information of polyunsaturated fatty acids of the training samples; training a ridge regression model by using the training characteristic data and the training target data to obtain a prediction model; obtaining SNP genotype information of a to-be-tested sample; obtaining a breeding value of the to-be-tested sample according to the SNP genotype information of the to-be-tested sample and the prediction model; and screening superior parents of carp with high total content of polyunsaturated fatty acids according to the breeding value. By the method, the breeding value of the to-be-tested sample can be directly obtained according to the SNP genotype information of the to-be-tested sample and the prediction model, and then the superior parents of carp with high content of polyunsaturated fatty acids can be screened from the to-be-tested sample, thereby providing excellent materials for new germplasm identification and breeding of carp.

The present invention relates to the use of alpha lipoic acid as feed additive for aquatic animals including fish and shrimp, especially for cold water fish as for example salmon, bream, bass and for warm water fish as for example carp, tilapia, catfish. More particular, this invention relates to the use of alpha lipoic acid for the improvement of the feed conversion ratio and / or daily weight gain in fish, for reducing mortality by regulating the micro flora of the gut and / or by protecting the animal against infections caused by pathogenic viruses.

The invention discloses gRNA (guide Ribonucleic Acid) and application thereof in constructing a new carp strain with a high 1alpha, 25-dihydroxy vitamin D3 level, belongs to the technical field of genetic engineering, and provides gRNA of which the nucleotide sequence is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the gRNA, and application of the gRNA to construction of the new carp strain with the high 1alpha, 25-dihydroxy vitamin D3 level. The invention relates to an application of gRN A in construction of a new carp strain with a high 1alpha, 25-dihydroxy vitamin D3 level. The gRNA is applied to construction of a fast-growing disease-resistant carp new strain with the high 1alpha, 25-dihydroxy vitamin D3 level. A target spot of the cyp24a1 gene of the carp with high specificity is designed, gRNA is synthesized in vitro, the cyp24a1 gene capable of degrading the 1alpha, 25 (OH) 2D3 is quickly knocked out, a new carp strain with the high 1alpha, 25-dihydroxy vitamin D3 level is obtained, the 1alpha, 25 (OH) 2D3 has the functions of promoting fish growth and improving fish immunity, and the carp has important application value.

This invention discloses an oral vaccine against carp spring viremia virus, its preparation method, and its application. The oral vaccine is constructed by linking a carp spring viremia virus antigen protein and a Yellow River carp defensin protein via a flexible linker to form a fusion protein, which is then expressed in *E. coli* and enriched using a biological vector. The gene sequence encoding this fusion protein includes a truncated fragment of the carp spring viremia virus antigen protein gene (64-1389 bp), a fragment of the Yellow River carp defensin protein gene (70-201 bp without the signal peptide), and a flexible linker fusion gene connecting the two gene fragments. Compared with previously reported anti-SVCV vaccines, the oral vaccine against carp spring viremia virus prepared in this invention does not harm the fish during the entire immunization process, is simple to operate, safe and stable, and has a higher protection rate. Therefore, this oral vaccine provides broad prospects for the prevention and control of carp spring viremia.

The system according to this embodiment aims to provide rapid and consistent evaluation of bonsai and Nishikigoi (ornamental carp). [Solution] The system according to the embodiment comprises a collection unit, an analysis unit, and an evaluation unit. The collection unit collects images of bonsai trees and koi carp. The analysis unit analyzes the images collected by the collection unit. The evaluation unit calculates an estimated value based on the analysis results obtained by the analysis unit.

The invention relates to the technical field of carp culture, in particular to a culture method for carps in a high water temperature period and application of the culture method. According to the method, a breeding period is divided into an earlier stage, a high water temperature period and a later stage according to water temperature, and floating expanded compound feed, granular compound feed and high-energy floating expanded compound feed are fed respectively; the feeding rate, feeding times and dissolved oxygen of the three stages are controlled. The invention further provides application based on the breeding method. The breeding period is divided according to the water temperature, different feeds are fed in stages, and the feeding rate, the feeding frequency and the dissolved oxygen amount are controlled in stages, so that the damage and death conditions caused by ingestion of the carps in the high-water-temperature period are reduced; the carps can grow quickly in the early stage, liver and gall function health care is conducted, good ingestion and growth speed is kept in the high-water-temperature period, and high-speed growth and weight gaining are conducted in the later stage; the survival rate in the high-water-temperature period, the weight increment and the growth speed in the whole process are remarkably improved, and the problems that the growth performance and the survival rate of carps are low when the carps are cultured in the high-water-temperature period in the southwest region are solved.

The application relates to the technical field of low-loss detection of carps, in particular to a low-loss detection method for the EPA+DHA content of carp muscle based on skin characteristic wavelengths. The method comprises the following steps: constructing a radial basis function neural network, obtaining a training set composed of multiple samples containing wavelength information and unsaturated fatty acid information, training the network to obtain a detection model, and inputting the wavelength information of fresh carp skin into the detection model to obtain the EPA+DHA content of carp muscle. The method can realize rapid low-loss detection and has high detection accuracy.

The present application relates to the field of biotechnology, and more particularly to a method for knocking out runx2b gene of heterologous octoploid Changfeng crucian and application thereof. The present application designs gRNA-1 and gRNA-2 aiming at all the alleles of runx2b from two sets of parent sources of heterozygous gynogenetic crucian and Xingguo red common carp in Changfeng crucian; the mixture of two gRNAs synthesized by in vitro transcription and Cas9 protein is introduced into the animal pole of single-cell stage of Changfeng crucian fertilized eggs by microinjection, which can efficiently knock out all the 8 alleles of runx2b-A and runx2b-B. Any gRNA can guide the CRISPR / Cas9 system to simultaneously edit the 8 alleles; gRNA-1 achieves 97.5% of synchronous knockout rate, and gRNA-2 achieves 95% of synchronous knockout efficiency; when the double gRNA collaborative cutting strategy is used, the complete editing efficiency of the 8 functional copies reaches 100%.

The invention discloses a method for identifying germ cells of grass carps and carps by using dazl genes. The method comprises the following steps: cloning dazl cDNA sequences of the grass carps and the carps, and performing comparative analysis by using DNAMAN software; designing a universal non-specific primer, a grass carp specific primer and a carp specific primer according to sequence differences; extracting total RNA (Ribonucleic Acid) of testis of 12-month-old grass carp and carp, and reversely transcribing into cDNA Respectively carrying out PCR (Polymerase Chain Reaction) amplification by using three pairs of primers by taking the cDNA as a template; carrying out 1% agarose gel electrophoresis and imaging on the PCR product, and identifying and distinguishing germ cells according to the size of a strip; the method is simple in structure and reasonable in design, high-specificity primers are designed based on small difference of dazl gene sequences for the first time, precise amplification is achieved by combining with optimized annealing temperature (60 DEG C / 65 DEG C), special instruments are not needed, operation is easy and convenient, sensitivity is high, grass carp germ cells in carp gonads after transplantation can be rapidly identified, development of the'belly borrowing reproduction 'technology is supported, and the method is suitable for large-scale popularization and application. The grass carp breeding period is remarkably shortened, and the important industrialization prospect is achieved.

The invention relates to the technical field of aquaculture feeds, in particular to a traditional Chinese medicine fish feed for improving skin texture of fancy carp, which is prepared from the following components in parts by weight: 40-60g of schisandra chinensis, 40-60g of platycladi seed, 50-70g of medlar, 15-20g of fructus amomi, 20-30g of agastache rugosus, 20-40g of mulberry, 1-5g of saffron crocus, 10-15g of black ant, 5-15g of gardenia, 15-20g of raw liquorice, 20-30g of pericarpium citri reticulatae and 20-40g of malt. The preparation method comprises the following steps: S1, pretreatment: carrying out ultraviolet sterilization for 20-40s; sterilizing with hot air at 60-80 DEG C; s2, drying: drying at a low temperature of 40-60 DEG C until the moisture is less than or equal S3, crushing: mechanically pulverizing, sieving with a 100-200-mesh sieve, repeating for 3-5 times, and uniformly stirring; s4, mixing: mixing and stirring with basic fish feed according to a ratio of 1: (5-10); and S5, packaging: bagging in vacuum in a dark place. The traditional Chinese medicine fish feed for improving the skin texture of the fancy carp has the beneficial effects of homology of medicine and food, safety and no toxicity; materials are easy to obtain, and cost is reduced; the fish physique is enhanced, the meat quality is improved, and the body type of the ornamental fish is improved; the skin texture of the fancy carp is improved and enhanced, and the fancy carp is bright, healthy and anti-stress.

The invention relates to bacillus cereus KHCP-1, and the preservation number of the bacillus cereus KHCP-1 is CGMCC (China General Microbiological Culture Collection Center) No. 33399. The strain can efficiently hydrolyze fish scales of the cyprinus carpio within 24 hours under the activation conditions that the temperature is 40 DEG C, the pH value of a fish scale powder culture medium is 5, and 0.2 g / L of ZnSO4 is contained, the soluble protein content at the fermentation end is 1324.375 micrograms / mL, and the fish scale hydrolysis rate is up to 75% or above. The invention also relates to a new application of the fermented filtrate obtained by degrading the fish scales of the cyprinus carpio by the bacillus cereus KHCP-1 as a cosmetic raw material, and the additional value of the fish scales of the cyprinus carpio is improved.

1. Name of the product in this design: Carp Model (3D Cloisonné Carp). 2. Purpose of this design: A creative carp model for display. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The invention discloses a parent mandarin fish rearing and fry opening rearing method suitable for northern areas. The parent breeding method in the method comprises the steps that from the beginning of February, high-quality mandarin fish parents are selected, prenatal parents are bred in a cement pond in a greenhouse, carps are used as bait fishes, breeding is conducted to the middle ten days of April, and male fishes and female fishes with good gonad development are selected for spawning induction. The fry opening breeding method comprises the steps that mandarin fish fries swim flatly three days after going out of the film, loach water spots are fed after the mandarin fish fries swim flatly for 60-75 h, and carp fries with the body length smaller than 60% of the mandarin fish fries are fed 7 days after feeding. According to the method, water temperature fluctuation can be avoided through parent fish breeding in a greenhouse cement pond, the stability of bait supply in northern areas can be guaranteed by taking carps as bait fishes, and more than 90% of mandarin fish parent gonads are promoted to develop to the V period. Loach water spots are selected as initial feed for the mandarin fish fries, and the initial feed is matched with spawning induction, so that the method is more suitable for climate of northern areas, feed supply can be guaranteed, and the survival rate of the mandarin fish fries in the initial stage can reach 80%.

The invention relates to bacillus cereus KHCP-1, and the preservation number of the bacillus cereus KHCP-1 is CGMCC (China General Microbiological Culture Collection Center) No. 33399. The strain can efficiently hydrolyze fish scales of the cyprinus carpio within 24 hours under the activation conditions that the temperature is 40 DEG C, the pH value of a fish scale powder culture medium is 5, and 0.2 g / L of ZnSO4 is contained, the soluble protein content at the fermentation end is 1324.375 micrograms / mL, and the fish scale hydrolysis rate is up to 75% or above. The invention also relates to a new application of the fermented filtrate obtained by degrading the fish scales of the cyprinus carpio by the bacillus cereus KHCP-1 as a cosmetic raw material, and the additional value of the fish scales of the cyprinus carpio is improved.

The application relates to a SNP molecular marker for identifying a carp body color trait, primers and application, relates to the technical field of genes, and the SNP molecular marker is located at the 21286787th base of B19 chromosome, and the base is C or G. If the genotype of the SNP molecular marker is CC or CG, the corresponding carp body color trait is normal body color, and if the genotype of the SNP molecular marker is GG, the corresponding carp body color trait is a rainbow cell deletion trait. The hybridization of the rainbow cell deletion trait of the carp is identified, the utilization of the body color trait improvement of the carp is accelerated through molecular marker assisted breeding, and the breeding benefit is improved.

The application discloses application of an Aeromonas vancleavei OmpW subunit vaccine as a vaccine for preventing bacterial septicemia of carps, and belongs to the technical field of genetic engineering and molecular immunology. The subunit vaccine protein is encoded by an Aeromonas vancleavei OmpW gene, the base sequence of the Aeromonas vancleavei OmpW gene is shown in the sequence table SEQ ID No. 1, the encoded protein is an Aeromonas vancleavei outer membrane protein OmpW subunit vaccine protein, and the amino acid sequence of the Aeromonas vancleavei outer membrane protein OmpW subunit vaccine protein is shown in the sequence table SEQ ID No. 4. After the subunit vaccine of the application is injected into carps, the fish body can be significantly stimulated to up-regulate expression of immune-related genes and produce a large amount of specific antibodies, the ability of the carps to resist Aeromonas vancleavei infection is effectively improved, and then bacterial septicemia of carps caused by Aeromonas vancleavei in carp culture is prevented.

To provide a carp streamer that can be used for an advertisement and publicity.SOLUTION: In a carp streamer, there is no scale drawn in a body 12 thereof, and instead of the scale, a pattern, a character, and / or a photograph is drawn.SELECTED DRAWING: Figure 2

The invention relates to the technical field of genetic breeding of aquatic animals, in particular to CBX7 protein related to high-temperature reversal of carps as well as a coding gene and application of the CBX7 protein. The invention identifies and analyzes the effect of the CBX7 protein in the carp high-temperature sex reversal, not only is beneficial to revealing the molecular mechanism of the carp high-temperature sex reversal, but also can provide a basis for establishing a sex detection marker and a directional breeding molecular tool, can realize sex detection of the carp sex reversal, can be applied to directional breeding for sex control, and has a wide application prospect. The method is especially suitable for breeding and popularization of all-female breeding.

The application discloses a SNP site for identifying high relative ovary weight carp parents and application thereof, and belongs to the technical field of molecular genetics and aquatic breeding. The application solves the technical problem that in the existing carp parent selection, there is no efficient molecular marker to accurately identify high relative ovary weight individuals. The application finds six SNP sites which are significantly related to the high relative ovary weight trait, and the six SNP sites are located at the 1914515th position of sequence NC_056581.1, the 17586713th position of sequence NC_056582.1, the 18667178th position of sequence NC_056592.1, the 23541881th position of sequence NC_056612.1, the 25898489th position of sequence NC_056597.1 and the 49224th position of sequence NW_024879353.1. The application realizes high-throughput molecular marker assisted selection of the carp parents, and improves the breeding efficiency and the breeding yield.

The invention relates to the technical field of biology, in particular to a method for efficiently knocking out a heterooctaploid Changfeng crucian carp runx2b gene and application. According to the invention, gRNA-1 and gRNA-2 are designed for all runx2b alleles from two sets of parent sources of allogynogenetic prussian carp and Xinguo red carp in Changfeng crucian carp; two gRNAs synthesized through in-vitro transcription are mixed with Cas9 protein, the mixture is introduced into animal poles of fertilized eggs of Changfeng carassius auratus in a single cell period in a microinjection mode, and all eight alleles runx2b-A and runx2b-B can be efficiently knocked out. Wherein any gRNA can guide the CRISPR / Cas9 system to edit eight alleles at the same time; the synchronous knockout rate of the gRNA-1 is 97.5%, and the synchronous knockout efficiency of the gRNA-2 is 95%; when a double-gRNA collaborative cleavage strategy is adopted, the complete editing efficiency of eight function copies reaches 100%.

The invention discloses an aquatic feed for improving the bioavailability of L-ascorbic acid-2-phosphate and a preparation method of the aquatic feed. The feed comprises L-ascorbic acid-2-phosphate, phytase, a natural high-phytic-acid plant raw material (such as full-fat rice bran, and the phytic acid content is greater than or equal to 1.0%) and an organic acidifying agent (such as citric acid). By controlling the molar ratio of phytic acid to Vc ester to be greater than or equal to 3: 1 and adjusting the pH value of the mixture to 4.0-5.0, advanced hydrolysis of phytase to the Vc ester is inhibited by utilizing the competitive property of phytic acid in the feed puffing processing process, so that the retention rate of the Vc ester is remarkably increased (up to 88%); in in-vitro simulated digestion (a buffer system with the pH value of 5.8), after phytic acid is preferentially degraded, released phytase efficiently hydrolyzes Vc ester, and the Vc release rate reaches 72%. Through comprehensive calculation, the total effective Vc utilization rate reaches up to 63.4% and is increased by 48% compared with a control group. The feed does not need an embedding process, is low in cost, is simple and convenient to operate, and is suitable for aquatic feeds for carps, grass carps, tilapia mossambica, penaeus vannamei and the like.

This invention discloses gRNA and its application in constructing a new carp strain with high 1α,25-dihydroxyvitamin D3 levels. Belonging to the field of genetic engineering technology, it provides a gRNA, the nucleotide sequence of which is shown in SEQ ID NO.1. The invention also discloses the application of gRNA in constructing a new carp strain with high 1α,25-dihydroxyvitamin D3 levels, specifically in constructing a fast-growing and disease-resistant carp strain with high 1α,25-dihydroxyvitamin D3 levels. Specifically, this invention designs a highly specific target site for the carp cyp24a1 gene, synthesizes gRNA in vitro, and rapidly knocks out the cyp24a1 gene that degrades 1α,25(OH)2D3, resulting in a new carp strain with high 1α,25-dihydroxyvitamin D3 levels. 1α,25(OH)2D3 promotes fish growth and enhances fish immunity, making this fish strain of significant application value.

The invention discloses a preparation method and a use method of a traditional Chinese medicine feed additive for culturing carps in a carbonate water area, and relates to a preparation method and a use method of a traditional Chinese medicine feed additive for culturing carps. The invention aims to solve the technical problems of slow growth, immune stress and even death of cultured carps caused by carbonate in a saline-alkali water area at present. According to the invention, the hedyotis diffusa / sculellaria barbata drug pair is added into the feed, so that the damage of carbonate stress to the immune system of the carp can be relieved by adjusting the activity of tissue immune related enzymes and inducing cell autophagy, and the immunocompetence of the carp in a carbonate water body is improved; the oldenlandia diffusa / sculellaria barbata drug pair is beneficial to relieving oxidative stress of the carps in the carbonate water area and removing damaged organelles, and finally is beneficial to improving the survival rate of the carps in the carbonate water area and enhancing the adaptive capacity of the carps to the saline-alkali environment.

The application belongs to the technical field of aquatic genetic breeding, and particularly relates to a breeding method of a few intermuscular bones strain of a carp based on whole genome selection. The reference population is established by determining the number of intermuscular bones of the carp, the candidate population is established, then the reference population and the candidate population are resequenced, and high-quality SNP typing results are obtained; the genetic parameters of the target traits are estimated according to the high-quality SNP typing results, the genomic breeding value (GEBV) of the candidate population is further estimated, the candidate individuals are selected as the parent fish according to the GEBV value, and the breeding of the few-spine F1 generation seedlings is carried out. The purpose of breeding the few-spine fine strain is achieved by only calculating the GEBV value through gene sequencing without killing the fish. The application develops a set of genome selection scheme suitable for the improvement of the number of intermuscular bones of the carp, the operation is clear, and a new molecular marker assisted breeding technology is provided for the breeding of the few-spine fine strain of the carp.

The invention discloses an SNP (Single Nucleotide Polymorphism) site for identifying a carp parent with high relative ovary weight and application of the SNP site, and belongs to the technical field of molecular genetics and aquatic breeding. The technical problem that an efficient molecular marker for accurately identifying individuals with high relative ovary weight is lacked in existing carp parent breeding is solved. According to the invention, six SNP (Single Nucleotide Polymorphism) sites, which are obviously related to the character of high relative ovary weight, are found and are respectively positioned at the 1914515 site of the sequence NC056581.1, the 17586713 site of the sequence NC056582.1, the 18667178 site of the sequence NC056592.1, the 23541881 site of the sequence NC056612.1, the 25898489 site of the sequence NC056597.1 and the 49224 site of the sequence NW024879353.1. According to the invention, high-throughput molecular marker-assisted selection of carp parents is realized, and the breeding efficiency and the breeding yield are improved.

The invention belongs to the technical field of fish genetic sex identification and sex control in aquatic genetic breeding, and particularly discloses a cyprinus carpio sex specific DNA marker and application thereof. The method comprises the following steps: systematically positioning a sex determination site of Cyprinus carpio by virtue of a whole genome re-sequencing technology, and screening to obtain a cyprinus carpio sex specific DNA (Deoxyribose Nucleic Acid) marker; the genotype sex of the fancy carp is detected under the condition that excessive damage is not caused to the fancy carp body by cutting the fin ray to detect the DNA marker, the gentle and minimally invasive fish sex identification method is developed, the method can accurately identify the sex of the fancy carp, meanwhile, damage to the fish is reduced, and the method has important industrial value and is worthy of popularization and application. And a new thought is provided for development of a fancy carp sex control breeding technology.