105 results about "Fatty acids.polyunsaturated" patented technology

The present invention provides a novel process for the production of aliphatic unsaturated C10-aldehyde compounds catalyzed by lipoxygenase (LOX) from a polyunsaturated fatty acid (PUFA) source. The invention also relates to the isolation and characterization of novel, preferably bifunctional LOXs from different algae sources, and the identification of structural and / or functional related LOXs from different bacterial sources. The invention also relates to the provision of enzyme mutants derived from said newly identified enzymes. Another aspect of the invention relates to the corresponding coding sequences of said enzymes, recombinant vectors and recombinant host cells, which are suitable for the production of such LOXs and for carrying out a new process for the production of aliphatic unsaturated C10-aldehyde compounds. Another aspect of the invention relates to the use of a specific aldehyde or aldehyde mixture obtained according to the invention as a flavoring ingredient or an ingredient of a food or feed composition.

The invention belongs to the technical field of grease modification and lipid deep processing, and particularly relates to a method for enriching n-3 polyunsaturated fatty glyceride by an enzymic method. According to the method, alkyl-modified hollow mesoporous silica spheres are used as an immobilized enzyme carrier and an emulsifier, grease rich in n-3 polyunsaturated fatty acid is used as a raw material in a stable Pickering emulsion system, saturated fatty acid and monounsaturated fatty acid are selectively hydrolyzed by lipase, and polyunsaturated fatty glyceride is enriched. The method has the characteristics of fast reaction, high yield, wide substrate application range and reusability of the immobilized enzyme, and the content of polyunsaturated fatty acid in the polyunsaturated fatty glyceride reaches 58.05-90.53%.

The invention belongs to the technical field of microorganisms, and particularly relates to schizochytrium limacinum and application thereof as well as a method and a product for producing omega-3 polyunsaturated fatty acid. The schizochytrium sp. SKA-2 is preserved in the China Center for Type Culture Collection on October 23, 2024, and the preservation number of the schizochytrium sp. SKA-2 is CCTCC (China Center for Type Culture Collection) NO: M 20242314. The Schizochytrium sp. SKA-2 can grow well under the low-salt condition, and when the Schizochytrium sp. SKA-2 is used for fermentation to obtain the omega-3 polyunsaturated fatty acid, the production speed is high, the fermentation time is short, and the content of the omega-3 polyunsaturated fatty acid is high.

The invention discloses a lamb feeding method for increasing polyunsaturated fatty acid in meat. According to the invention, lactobacillus reuteri is supplemented into the basic feed for sheep, so that polyunsaturated fatty acids in mutton can be increased, the tenderness of the mutton can be improved, and the proportion and area ratio of I-type muscle fibers in muscles can be increased. Meanwhile, the technical scheme also can regulate the rumen microbial community structure of the sheep, promote the degradation of rumen carbohydrates and the generation of muscle aromatic compounds, and regulate the metabolism of sheep muscle proteins. The proper addition amount of lactobacillus reuteri in the basic feed for sheep is determined, the obtained optimized feed can improve polyunsaturated fatty acid and tenderness of mutton, the slaughter performance of sheep is enhanced, and the economic income of pasturing households and the economic benefit level of enterprises can be improved.

This document relates to methods and materials for generating oilseed (e.g., pennycress) plants that have oil with reduced levels of polyunsaturated fatty acids (PUFAs) and / or increased levels of oleic acid. For example, oilseed plants having reduced expression levels of one or more polypeptides involved in fatty acid biosynthesis (e.g., fatty acid desaturase 2 (FAD2) and reduced oleate desaturation 1 (ROD1)), as well as methods and materials for making and using such oilseed plants are provided.

The invention discloses a method for screening carp dominant parents with high total polyunsaturated fatty acid content. The method comprises the steps that training feature data and training target data are obtained, the training feature data comprise SNP genotype information of a training sample, and the training target data comprise polyunsaturated fatty acid total content information of the training sample; training the ridge regression model by using the training feature data and the training target data to obtain a prediction model; obtaining SNP genotype information of a to-be-detected sample; obtaining a breeding value of the to-be-detected sample according to the SNP genotype information of the to-be-detected sample and the prediction model; and screening the carp dominant parents with the total content of polyunsaturated fatty acids according to the breeding values. According to the method, the breeding value of the carp can be directly obtained according to the SNP genotype information of the sample to be detected and the prediction model, so that dominant parents with high carp polyunsaturated fatty acid content are screened from the sample to be detected, and an excellent material is provided for new carp germplasm identification and breeding.

The invention discloses a separation process of EPA-EE and DHA-EE, and relates to the technical field of separation and purification of polyunsaturated fatty acid ethyl ester. The invention discloses a separation process of EPA-EE and DHA-EE, which comprises the following steps: firstly, carrying out reflux reaction on deep sea crude fish oil by using absolute ethyl alcohol and strong base anion exchange resin, carrying out centrifugal separation, and collecting an oil phase; adding the oil phase into molecular distillation to obtain a raw material solution; performing chromatographic separation on the raw material solution to obtain an EPA-EE raffinate and a DHA-EE extract; and carrying out reduced pressure distillation on the EPA-EE raffinate and the DHA-EE extract, and concentrating to obtain dried EPA-EE and DHA-EE products. The EPA-EE and DHA-EE obtained by the separation process disclosed by the invention are detected by a nuclear magnetic resonance spectrometer and a gas chromatograph, and the purity of the products reaches 99%.

The disclosure provides methods for increasing saturated, monounsaturated, and poly unsaturated fatty acid production using the PUFA gene cluster plasmid and the gene regulator pfaR.

The invention provides a schizochytrium limacinum mutant strain and application thereof. The schizochytrium limacinum mutant strain is a schizochytrium limacinum mutant strain CABIO-A-2-VII, the preservation number of the schizochytrium limacinum mutant strain is CCTCC M 20242741, and the schizochytrium limacinum mutant strain is preserved in the China Center for Type Culture Collection. The EPA content in polyunsaturated fatty acid grease obtained by fermentation of the schizochytrium limacinum mutant strain CABIO-A-2-VII provided by the invention is greatly increased, the C14: 0 content is higher, the DHA content in grease obtained by using the strain under a conventional fermentation process is greater than or equal to 40%, the EPA content is greater than 2%, and the C14: 0 is greater than or equal to 12% (the C14: 0 can reach 19% under an optimal fermentation process).

The application discloses a method for screening superior parents of carp with high total content of polyunsaturated fatty acids. The method comprises obtaining training characteristic data and training target data, wherein the training characteristic data comprises SNP genotype information of training samples, and the training target data comprises total content information of polyunsaturated fatty acids of the training samples; training a ridge regression model by using the training characteristic data and the training target data to obtain a prediction model; obtaining SNP genotype information of a to-be-tested sample; obtaining a breeding value of the to-be-tested sample according to the SNP genotype information of the to-be-tested sample and the prediction model; and screening superior parents of carp with high total content of polyunsaturated fatty acids according to the breeding value. By the method, the breeding value of the to-be-tested sample can be directly obtained according to the SNP genotype information of the to-be-tested sample and the prediction model, and then the superior parents of carp with high content of polyunsaturated fatty acids can be screened from the to-be-tested sample, thereby providing excellent materials for new germplasm identification and breeding of carp.

This invention relates to the field of fatty acid detection technology, specifically to a method for determining 11 polyunsaturated fatty acids based on LC-MS / MS. The method includes: preparing a calibration solution; hydrolyzing bound fatty acids in a blood sample, adding a precipitant, centrifuging, and collecting the supernatant as the test sample; detecting the test sample using liquid chromatography-tandem mass spectrometry, and substituting the detection results into the calibration curve equation to obtain the content of the 11 polyunsaturated fatty acids in the test sample. This method can rapidly and accurately detect the content of 11 polyunsaturated fatty acids, providing a more accurate basis for clinical diagnosis and assisting in the early detection and treatment of diseases; at the same time, it can also provide more targeted nutritional advice to individuals, promoting health management.

To provide a means effective in suppressing oxidative deterioration of fats and oils containing polyunsaturated fatty acid containing DHA and to provide a method capable of determining near olfactory sense of human being by specifying a component that becomes an index of oxidative deterioration of fat and acid containing DHA.SOLUTION: A lipid composition containing lipids composed of polyunsaturated fatty acids as constituent fatty acids, wherein a composition ratio of polyunsaturated fatty acids among all constituent fatty acids of the lipids is 15% or more, the moisture content is 600 ppm or less, perfluorooctanesulfonic acid is 0.1 ppb or less, and perfluorooctanoic acid is 0.1 ppb or less. It is preferable that the composition contains 2-(2-pentenyl)furan at less than 0.0095 ppm, and 2,4-heptadienal at less than 2.600 ppm.SELECTED DRAWING: None

Deuterated polyunsaturated fatty acid (“PUFA”) compounds, compositions, and uses of the compounds for reducing lipid autooxidation and the treatment of various diseases and conditions are provided.

The invention provides a method for producing polyunsaturated fatty acid, and belongs to the technical field of biological fermentation. The method for producing polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid from schizochytrium limacinum comprises the following steps: activating schizochytrium limacinum in a seed culture medium to obtain a schizochytrium limacinum seed culture solution; inoculating the schizochytrium limacinum seed culture solution into a fermentation culture medium for culturing, wherein the fermentation culture medium comprises rapeseed protein powder; and extracting the culture solution in the cultured fermentation culture medium to obtain the polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid. According to the present invention, the yield of polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid can be significantly improved.

Fatty acids with one or more unsaturations and an odd-numbered hydrocarbon chain are described, wherein these fatty acids have the chemical structure of the therapeutically active metabolites of alpha-hydroxylated mono- or polyunsaturated fatty acids with an even-numbered chain. The compositions comprising these fatty acids, their medical uses, and their use as indicators of a patient's efficacy and / or response to treatment with the alpha-hydroxylated mono- or polyunsaturated fatty acids with an even-numbered chain from which they are derived are also described.

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

PROBLEM: To provide a composition comprising highly enriched PUFA or its alkyl esters while containing fatty acid esters of 3-MCPD at adequately low concentrations and to provide an efficient method for producing the composition. MEANS FOR SOLVING: A composition that contains fatty acids or fatty acid alkyl esters as its major component, the composition containing highly unsaturated fatty acid or alkyl ester thereof, wherein the proportion of the highly unsaturated fatty acid in the constituent fatty acids of the composition is 50 area % or more and wherein the concentration of 3-MCPD as found upon analyzing the composition by American Oil Chemists' Society official method Cd 29b-13 assay A is less than 1.80 ppm.

The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.

The invention provides a feed additive for increasing the content of n-3 polyunsaturated fatty acids in pork and a compound feed. The feed additive consists of spartina alterniflora powder and a bacterial enzyme additive, the bacterial enzyme additive is prepared from lactobacillus plantarum, aspergillus niger, yarrowia lipolytica, bacillus subtilis, cellulase, beta-mannase and protease; the compound feed comprises a basic ration and the feed additive. Through development and utilization of spartina alterniflora powder and practice, waste is turned into wealth, a new source of the feed additive is expanded, and the feed additive is added into daily ration, so that the digestibility of neutral detergent fibers of the daily ration is improved, the antioxidant capacity of serum is enhanced, and the contents of alpha-linolenic acid, arachidonic acid and eicosapentaenoic acid in pork are increased.

The invention discloses an application of a feed additive for changing fatty acid composition of yolk lipid in feed. According to the invention, glucurolactone is added into poultry feed to regulate and control the content of polyunsaturated fatty acid and monounsaturated fatty acid in yolk lipid, wherein the polyunsaturated fatty acid comprises n-3 series polyunsaturated fatty acid; by adding glucurolactone into the feed, the fatty acid composition of egg yolk lipid can be changed, and by regulating and controlling the content of polyunsaturated fatty acid and monounsaturated fatty acid in the egg yolk lipid, especially the content of n-3 series polyunsaturated fatty acid, not only is the functional nutritional value of egg yolk increased, but also the quality of eggs is improved; meanwhile, the flavor of the egg yolk can be increased.

Genetically engineered yeast cells having disruption of pex3, pex5 or pex20 genes and combinations thereof having reduced beta-oxidation and methods for increasing polyunsaturated fatty acids are disclosed.

Disclosed herein is a method for increasing the plasma EPA:DHA ratio in a non-human animal by feeding the animal with a polyunsaturated fatty acid diet rich in DHA. An example of such a polyunsaturated fatty acid diet rich in DHA contains at least 0.6% DHA by weight of the feed. Also disclosed herein is an animal feed composition comprising polyunsaturated fatty acids rich in DHA. An example of such an animal feed composition is pet food.

The present application relates to the technical field of microcapsules, and in particular to a polyunsaturated fatty acid oil microcapsule, a preparation method therefor, and a use thereof. The present application provides a polyunsaturated fatty acid oil microcapsule, the raw material of which comprises polyunsaturated fatty acid oil, wherein an anisidine value of the polyunsaturated fatty acid oil is not greater than 10. It is found in the present application that the anisidine value of the polyunsaturated fatty acid oil will affect the solubility of the microcapsule containing the polyunsaturated fatty acid oil, and the solubility of the polyunsaturated fatty acid oil microcapsule can be effectively improved by controlling the anisidine value of the polyunsaturated fatty acid oil within a range of not higher than 10, thereby solving the problems that a product containing the polyunsaturated fatty acid oil microcapsule has poor solubility and is prone to generation of insoluble particles, and improving the quality of the product containing the polyunsaturated fatty acid oil microcapsule.

The present invention relates to a method for producing hyper-ozonised fatty acids from the high-grade ozonization of extra virgin sunflower oil comprising a high concentration of polyunsaturated fatty acids. Likewise, the present invention relates to the hyper-ozonised fatty acids produced by means of said method and to the cosmetic and medical use thereof, particularly due to their restorative effect on skin damage.

To provide an oil which does not become cloudy when stored at a low temperature while containing a specific amount of palmitic acid.SOLUTION: An oil containing a specific amount of palmitic acid has at least one characteristic selected from the group consisting of (i) a predetermined triglyceride content, (ii) a predetermined PUFA content, (iii) a predetermined stearic acid content, (iv) a predetermined content of C18 fatty acids, and (v) a predetermined DHA / EPA ratio. The oil does not become cloudy during low-temperature storage.SELECTED DRAWING: Figure 1

A frozen aerated confection comprising water and a fat component, wherein the fat component is present in an amount of from 1 to 15 wt%, and wherein the fat component comprises i) a fat blend having a triglyceride composition of from 30 to 80% saturated fatty acids, less than 30% polyunsaturated fatty acids and the remainder being monounsaturated fatty acids, and ii) a wax ester.

The present invention belongs to the technical field of oil refining, and specifically relates to a method for removing mineral oil from oil and fat, and the resulting oil and fat. The method comprises the following steps: introducing steam into the oil and fat under vacuum conditions for treatment; wherein the total amount of steam introduced is 15% to 95% of the mass of the oil and fat. The method for removing mineral oil from oil and fat provided by the present invention is particularly advantageous for removing mineral oil from polyunsaturated fatty acid oils and fats. It can effectively and simply remove mineral oil from oil and fat without changing the existing oil and fat production process steps, and obtain oil and fat with a mineral oil content of less than 5 ppm.

The present invention belongs to the field of dairy cow performance measurement and milk quality detection, and discloses a method for rapid batch detection of polyunsaturated fatty acids in milk by mid-infrared spectroscopy. In terms of the selection of characteristic bands, it breaks the commonly used method of using algorithms to screen features, and instead uses a method of manual selection + multiple traversals. Finally, characteristic bands for modeling are selected, especially the absorption region containing part of water is screened out, and it is proved that adding part of the water absorption band can improve the accuracy of the model. The optimal preprocessing and algorithm combination for the establishment of the polyunsaturated fatty acid model is selected, the optimal parameters are determined, and the accuracy of the model is improved. Rapid, accurate, and low-cost detection of the polyunsaturated fatty acid content in raw milk is achieved, and rapid batch detection is realized.

The application provides a polyunsaturated fatty acid oil microcapsule and a preparation method. The preparation method of the polyunsaturated fatty acid oil microcapsule comprises the following steps: (1) adding a water retention agent into an aqueous solution of a water-soluble protein wall material, and uniformly mixing to obtain an aqueous phase; (2) adding polyunsaturated fatty acid oil into the aqueous phase, and shearing and emulsifying; (3) adding cellulose and a derivative thereof into the system obtained in the step (2), and shearing to obtain an emulsion; the content of the cellulose and the derivative thereof in the polyunsaturated fatty acid oil microcapsule is 0.05-0.3 wt%; the content of the polyunsaturated fatty acid oil in the polyunsaturated fatty acid oil microcapsule is not less than 40 wt%, and the content of a fatty acid with a carbon chain length of more than 20 and an unsaturated double bond of more than 4 in the polyunsaturated fatty acid oil is not less than 40%. The high oil-loading microcapsule product obtained by the preparation method has good sensory and good stability during a shelf life.