2 results about "Erogorgiaene" patented technology

The present invention pertains to novel modified terpene synthases and their use for a preparation method for pseudopterosin intermediates and / or pseudopterosins. The method is based on the use of a modified terpene synthase comprising at least one modified amino acid residue, which enables a terpene synthase-catalyzed increased production of pseudopterosin intermediates and / or pseudopterosins from Geranylgeranyl pyrophosphate as starting material. The new modified terpene synthases of this invention allow the production of pseudopterosin intermediates, such as Isoelisabethatriene A, Isoelisabethatriene B, Erogorgiaene, or Seco-Pseudopterosin and / or the production of pseudopterosins, such as Pseudopterosin A, in a cost-efficient, economical, and sustainable manner. Also provided are nucleic acids, encoding for the modified terpene synthases of this invention, as well as expression vectors capable of expressing such nucleic acids and host cells comprising the same.

The invention belongs to the technical field of organic synthesis, and particularly relates to a synthesis method of an active marine natural product gorgoniaene (+)-Erogoniaene. According to the present invention, the cheap and easily available brominated naphthalene compound is adopted as the starting material for the first time, the palladium catalyst is adopted to catalyze to achieve the bifunctionalization of the hydronaphthalene, the homemade TADDOL type chiral ligand is adopted to achieve the expected asymmetric catalysis, and the green chemical synthesis technology such as the photoreaction is adopted to achieve the side chain installation and the functional group modification; and finally, the synthesis of the (+)-Erogongiaene is completed by nine steps. According to the synthesis method, other complex fragments do not need to be prepared in advance, related reagents are simple and easy to obtain, reaction conditions are mild, the reaction conversion rate is good, the overall route is simple and efficient, and the synthesis method is suitable for industrial kilogram-level preparation and production of the (+)-Erogongiaene.