283 results about "Triacylglycerol VLDL" patented technology

A process for modifying an unsaturated polyol fatty acid ester stock, such as an unsaturated triacylglycerol oil, to enhance its reactivity provided. The method includes reacting the unsaturated polyol fatty acid ester stock with an oxygenating agent, such as an oxygen-containing gas. Tempering oils containing reactive polyol fatty acid esters and methods for their production and use are also provided.

Nucleic acid compositions encoding polypeptide products with diglyceride acyltransferase activity, as well as the polypeptide products encoded thereby and methods for producing the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications, as well as in treatment therapies and in the production of triacylglycerols.

The invention relates to a novel elongase gene with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and / or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and / or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and / or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

Synthetic lipid composition in which the content and the distribution of the fatty acids are similar to those of human milk fat, containing less than 2% by weight of free fatty acids, in which palmitic acid is predominantly at the 2-position of the triacylglycerols and the arachidonic and docosahexaenoic acids are distributed between the 1-, 2- and 3-positions and in particular predominantly at the 2-position of the triacylglycerols.

Simultaneous synthesis and purification of a fatty acid monoester biodiesel fuel from a triacylglycerol feedstock is described. In an exemplary method, the triacylglycerol feedstock is continuously contacted with a catalytic chromatographic bed comprising a first (solid phase) basic catalyst through a first port of a simulated moving bed chromatographic apparatus. A monohydric alcohol and optional second (mobile phase) basic catalyst is continuously contacted with the catalytic chromatographic bed through a second port and pumped in a first direction toward the triacylglycerol feedstock to contact the triacylglycerol in a reaction zone of the catalytic chromatographic bed where the fatty acid monoester and glycerol coproduct are formed. The fatty acid monoester is removed from the reaction zone through a product port of the simulated moving bed apparatus. Segments of the catalytic chromatographic bed are incrementally moved in a second direction, opposite the first direction, and the glycerol is removed from a raffinate port located opposite the product port of the apparatus.

The present invention is directed to a process to remove impurities from triacylglycerol oil including mixing the oil and a fluidic agent, pumping the mixture through a flow-through hydrodynamic cavitation apparatus at a pre-determined inlet pump pressure, creating hydrodynamic cavitation in the mixture, maintaining the hydrodynamic cavitation for a pre-determined period of time, moving the impurities from the oil to the fluidic agent, and then separating the fluidic agent from the oil. The impurities can include phytosterols, sterol glucosides, acylated sterol glucosides, in which case the fluidic agent is water, an alkali hydroxide, an inorganic base, an organic base, phosphoric acid, citric acid, acetic acid or a mixture thereof. The impurities may also include phosphatides, in which case and the fluidic agent comprises water and an enzyme such as phospholipase, a lipid acyltransferase or a mixture thereof.

The present invention relates to methods of producing modified fatty acids comprising a functional group which is a hydroxyl group, an epoxy group, an acetylenic group or a conjugated double bond. For example, seeds, seedoil and methods of making seedoil are provided wherein at least 23% (mol %) of the fatty acid content of the seed or seedoil comprises the functional group. Also provided are novel polypeptides, and polynucleotides thereof, which can be used to produce the modified fatty acids, particularly in transgenic plants and cells suitable for fermentation.

This invention relates to a new elongase gene having the sequence SEQ ID NO: 1 or its derivatives, to a gene construct comprising this sequence or its derivatives and to its use. The inventive nucleic acid sequence encodes a polypeptide which elongates α-linolenic acid (C18:3 d9, 12, 15) by at least two carbon atoms whereas γ-linolenic acid (C18:3 d6, 9, 12) is not elongated. The invention additionally relates to vectors or organisms comprising an elongase gene having the sequence SEQ ID NO: 1 or its derivatives. The invention further relates to a process for the production of polyunsaturated fatty acids (=PUFAs) with an organism which comprises the elongase gene and which organism produces high amounts of oils and especially oils with a high content of unsaturated fatty acids. Additionally the invention relates to an oil and / or fatty acid composition with a higher content of polyunsaturated C20 or C22 fatty acids with at least two double bonds and / or to a triacylglycerol composition with a higher content of said polyunsaturated fatty acids.

The invention relates to a novel elongase gene with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and / or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs.Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and / or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and / or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

The present invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which code for polypeptides with acyl transferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences which code for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and / or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids.The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences according to the invention, vectors comprising the nucleic acid sequences and / or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and / or vectors.A further part of the invention relates to oils, lipids and / or fatty acids produced by the process according to the invention and to their use.

The present invention relates to production of fuels or fuel blendstocks from renewable sources. Various embodiments provide a method of producing a hydrocarbon product by hydrotreating a feedstock including at least one of a renewable triacylglyceride (TAG), renewable free fatty acid (FFA), and renewable fatty acid C1-C5 alkyl ester (C1-C5 FAE) in the presence of a nonsulfided hydrotreating catalyst to produce a first product including hydrocarbons. In some examples, the first product can be subjected to further chemical transformations such as aromatization, cracking, or isomerization to produce a second product including hydrocarbons. In various embodiments, the first or second hydrocarbon product with minimal or substantially no further processing can be suitable as a liquid transportation fuel or fuel blendstock, including fuels such as gasoline, naptha, kerosene, jet fuel, and diesel fuels.

The present invention relates to a lipid composition comprising at least 5 wt % of a structuring agent dispersed in oil or fat wherein the structuring agent comprises at least 50 wt % of triacylglycerol containing a glycerin skeleton esterified with one type of saturated fatty acid having a chain length of either 10:0, 12:0 or 14:0. Further aspects of the invention are: the structuring agent, a food product comprising the lipid composition, the use of the lipid composition as a structure stabilizer or a moisture barrier in a food product; and a method for preparing the lipid composition.

The present lipid-based wax compositions commonly include a polyol fatty acid ester component (made up of partial and / or completely esterified polyols). Generally, at least a portion of the polyol fatty acid ester has been subjected to a transesterification reaction. Lipid-based wax compositions having a melting point of about 48° C. to about 75° C. can be particularly advantageous for use in forming candles. The wax may contain other components such as mineral wax, plant wax, insect wax, and / or other components. The polyol fatty acid ester component can include triacylglycerols such as those derived from plant oils (soybean oil, palm oil, etc.). The polyol ester component may be characterized based on one or more of its physical characteristics, such as SFI-40, SFI-10, typical crystal structure, IV, melting curve, and / or other properties.

Nucleic acid compositions encoding mammalian DGAT2alpha polypeptide products with diglyceride acyltransferase activity, as well as the mammalian DGAT2alpha polypeptide products encoded thereby and methods for producing the same, are provided. The subject DGAT2alpha polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications, as well as in treatment therapies and in the production of triacylglycerols.

The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, Mortierella ramanniana DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinannt techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

This invention relates to a Group 8 catalyst compound for the metathesis ofThis invention also relates to process to make alphaolefins comprising contacting an olefin, such as ethylene, with a feed oil containing a triacylglyceride (typically a fatty acid ester (such as methyl oleate)) with a Group 8 catalyst compound. The fatty acid ester may be a fatty acid methyl ester derived from biodiesel.

The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.

The production of transgenic plant oils is described by transfecting plant host cells with heterologous transcription inducers. Such inducers are specific for endogenous biosynthetic plant oil genes, such that the inducers induce an overexpression of the plant oil genes. The overexpressed gene product (i.e., for example, diacylglycerol transferase) thereby results in a increased intracellular production of a plant oil (i.e., for example, triacylglycerol). In one embodiment, the transfected plant host cell can be a plant algae species (i.e., for example, Chlamydomonas reinhardtii).

The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, Mortierella ramanniana DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

A method for reducing the likelihood of developing non-alcoholic steatohepatitis (NASH) in an individual diagnosed with non-alcoholic fatty liver disease involves providing in the gut of an individual a population of beneficial bacteria selected from the group consisting of Lactobacillus species, and at least 6 grams per day of fiber to the individual to maintain a therapeutically effective amount of the beneficial bacteria in the gut of the individual. In certain embodiments, monoacylglycerolacyltransferase-3 (MGAT3) synthesis is inhibited to lower triacylglycerol (TAG) production, while in others, expression of diacylglycerolacyltransferase-2 (DGAT-2) is inhibited. The beneficial bacteria are preferably modified to produce increased amounts of butyrate and are also encapsulated in a frangible enclosure. Levels of Roseburia are preferably increased while the levels of Akkermansia spp. in the individual's gut microbiome are reduced.

A method is disclosed for obtaining a refined lipid composition comprising at least one polyunsaturated fatty acid from a microbial biomass, wherein the refined lipid composition comprises at least one polyunsaturated fatty acid and is enriched in triacylglycerols relative to the oil composition of the microbial biomass.

A composition of at least one diacylglycerol and at least one phytosterol and / or phytostanol ester dissolved or dispersed in an edible oil and / or edible fat, and further optionally containing monoglycerides. Phytosterol esters may be beta-sitosterol, campesterol, stigmasterol and brassicasterol esters. Phytostanol esters may be stigmasterol, campestanol and sitostanol. The phytosterol and / or phytostanol esters are preferably esters of C14-C22, preferably C16-C18 saturated or unsaturated fatty acids, particularly oleic, linoleic, linolenic, palmitic and stearic acids. The diacylglycerol(s) are preferably 1,3-di-fatty acyl glycerol(s). A process for preparing compositions enriched with triacylglycerol(s) and phytosterol and / or phytostanol esters of mainly unsaturated fatty acid(s) or of mainly saturated fatty acid(s). Dietary food supplements and alimentary products derived from the above compositions.

Provided are surprisingly beneficial osmotic stress shock methods for facilitating disruption and / or extraction of microorganisms, comprising a synergistic combination of at least one hypotonic shock and at least two hypertonic shocks, wherein the last shock is a hypertonic shock. Preferred aspects of the invention relate to facilitating disruption of a broad spectrum of microorganisms (e.g., algae, bacteria, yeast, fungus, etc.). In particular aspects the microbial cells are subjected to: a hypertonic / hypotonic / hypertonic tertiary shock; a hypotonic / hypertonic / hypertonic tertiary shock; or a hypotonic / hypertonic / hypotonic / hypertonic quaternary shock. Particular aspects further comprise disrupting and / or extracting of the shocked microbial cells, and isolating a cellular constituent or bioproduct (e.g., biofuel, biocrude, bioenergy, biogas, biodiesel, bioethanol, biogasoline, pharmaceuticals, nutraceuticals, food, vitamins, feedstock, dyes, colorants, sulfur, fertilizer, bioplastic) therefrom. In particular preferred aspects, facilitation of algae disruption and / or extraction is provided and in certain embodiments, isolation of at least one of lipid, oil, and triacylglycerol is enhanced.

The invention relates to a pharmaceutical composition having the following constituents: azelaic acid, polyacrylic acid, triacylglyceride, propylene glycol, polysorbate, soya lecithin, water and salts. The composition is a hydrogel which is suited for the treatment of rosacea, presbyderma, melasma or skin irritations.

The present invention is directed to a method of treating patients with major depression by administering omega-3 fatty acids. These may be administered in a substantially purified form, as part of a pharmaceutical composition, or as part of a larger molecule, e.g., a triacylglycerol, which releases free fatty acid after ingestion by a patient.The present invention is also directed to triacylglycerols which are esterified at the gamma cardon of glycerol to phosphocholine and at either the alpha or beta carbon of glycerol to an omega-3 fatty acid. These “omega-3 phoshatidylcholines” are also used in the treatment of patients with major depression.

A method of producing a hydrocarbon product by hydrotreating a feedstock comprising triacylglyceride (TAG) in the presence of a nonsulfided hydrotreating catalyst to produce a first product comprising hydrocarbons. A method of producing a transportation fuel by selecting an undoped feedstock comprising virgin TAG, used TAG, or a combination thereof; hydrotreating the undoped feedstock in the presence of an unsulfided hydrotreating catalyst to produce a first product; and subjecting the first product to at least one process selected from aromatization, cyclization, and isomerization; to produce a second hydrocarbon product selected from gasoline, kerosene, jet fuel, and diesel fuels.