40 results about "Glycerophospholipid" patented technology

Disclosed herein is a lipid preparation, said preparation comprising a phosphatidylserine moiety, and poly-unsaturated fatty acid (PUFA) acyl groups, particularly long-chain poly-unsaturated fatty acid (LC-PUFA) acyl groups such as omega-3 and / or omega-6 acyl groups, wherein said PUFA is covalently bound to said glycerophospholipid. Said lipid preparations are particularly useful in the treatment of mental and cognitive disorders, e.g. ADHD (attention deficit hyperactivity disorder) and Alzheimer's disease. The disclosed preparations present improved bioactivity, and are useful in the treatment of various cognitive and mental conditions and disorders, as well as for maintenance of normal functions of brain-related systems and processes.

Disclosed herein is a lipid preparation, said preparation comprising a phosphatidylserine moiety, and poly-unsaturated fatty acid (PUFA) acyl groups, particularly long-chain poly-unsaturated fatty acid (LC-PUFA) acyl groups such as omega-3 and / or omega-6 acyl groups, wherein said PUFA is covalently bound to said glycerophospholipid. Said lipid preparations are particularly useful in the treatment of mental and cognitive disorders, e.g. ADHD (attention deficit hyperactivity disorder) and Alzheimer's disease.The disclosed preparations present improved bioactivity, and are useful in the treatment of various cognitive and mental conditions and disorders, as well as for maintenance of normal functions of brain-related systems and processes.

The invention discloses recombinant escherichia coli, a method for preparing phospholipase C and application. The recombinant escherichia coli BL21(DE-3)-pET-28a-L.m-phospholipase C (PLC) of which the target gene is from listeria monocyogenes (L.monocytogenes) is utilized as a fermentation strain to carry out liquid fermentation, so as to prepare phospholipase C. The method disclosed by the invention is simple in enzyme production process, short in production cycle and low in cost. A glycerophosphate bond on glyceryl phosphatide C3 can be specifically hydrolyzed to produce diglyceride (DAG) by using recombinant phospholipase C; plant crude oil (soybean oil, colleseed oil, rice bran oil and the like) is degummed by phospholipase C.

Peptide-lipid constructs of the structure L-S—F are disclosed, where F is a peptide, S is a spacer covalently linking F to L via an oligomer of ethylene glycol, and L is a diacyl- or dialkyl-glycerolipid (including glycerophospholipids). The spacer ideally has 6 to 14 ethylene glycol repeats, corresponding to PEG with a molecular weight of approximately 250 to 600. Also disclosed is a method of detecting reactive antibodies in serum by contacting serum with cells modified to incorporate a peptide-lipid construct, where the peptide is an epitope of the antibody, and determining the degree of aggluthination of the cells.

The invention belongs to the field of pesticide aids, and provides a modified liposome and a composition, and preparation methods and applications of the modified liposome and the composition. The preparation method of the modified liposome comprises: adding a mixture of glycerophospholipid, sterol and a polyhydroxy compound into a reaction kettle as an initiator under the action of a catalyst, carrying out heating dehydration, introducing ethylene oxide, and carrying out ring-opening polymerization, aging and neutralization to prepare the product, wherein the polyhydroxy compound is a composition of alcohol and alcohol amine. The invention further provides a liposome composition prepared from the modified liposome, wherein the liposome composition as a pesticide aid has advantages of easydispersion in water, good vesicle stability, easy biodegradation, stable properties, good conduction effect on targets and easy deposition of pesticide liquids on targets, and can be widely used in the field of pesticides.

The invention discloses a melt-in-the-mouth cereal bar and a preparation method thereof. According to the main point of the technical scheme, the melt-in-the-mouth cereal bar comprises the follow components: puffed purple rice, puffed white rice, puffed yellow rice, chia seeds, protein powder, sugar powder, coconut milk powder, oat powder, yoghurt powder, high fructose corn syrup, isomaltooligosaccharide syrup, resistant dextrin liquid, glycerol, phospholipid and sunflower seed oil. By using puffed purple rice, puffed white rice and puffed yellow rice as main raw materials, adjusting the dosage of puffed particles and cooperating with coconut milk powder, yogurt powder, protein powder and chia seeds, the cereal bar contains rich protein and amino acids, and the energy of the cereal bar isincreased; moreover, the prepared cereal bar has the melt-in-the-mouth taste, and is especially suitable for children to eat.

Disclosed is a glyceroglycolipid lipase which is highly safe, can hydrolyze a neutral fat, a glycerophospholipid or a glyceroglycolipid at about pH 6, is thermally stable to some extent, can hydrolyze lecithin, cannot hydrolyze lysolecithin, can rise a bread when used singly in the production of the bread, and has no unpleasant odor. Specifically disclosed is a glyceroglycolipid lipase derived from a filamentous bacterium Aspergillus japonicus.

The invention discloses a method for preparing glycerol phosphatidylcholine from soybean powder phospholipid, wherein the method comprises the following steps of: 1) mixing methanol and ethanol to prepare mixture M1; 2) adding soybean powder phospholipid into the mixture M1 to prepare a mixture M2; 3) adding sodium methoxide into the mixture M2 to conduct mixed action at a temperature of 30 to 50DEG C to prepare a reaction liquid M3; 4) after adding ethanol into the reaction solution M3, removing the ethanol layer to obtain the crude extract M3; 5) exchanging resin column of the crude extractM3 sequentially with a silica gel column and an ion exchange, evaporating and concentrating to obtain glycerol phosphatidylcholine. The soybean powder phospholipid used as a raw material is realized,and the yield effect of the soybean powder phospholipid can be improved.

The invention discloses a biomarker and a method for predicting premature rupture of fetal membranes, and the biomarker is selected from one or more of 4-methylvaleraldehyde (isohexanal), sphingolipids (SP) metabolite, glycerolipids (GL) metabolite and glycerophospholipids (GP) metabolite; the biomarker has a change in content difference multiple between a pregnant female body and a healthy pregnant female body at risk of premature rupture of fetal membranes. The biomarker for predicting premature rupture of fetal membranes and the prediction method have important significance for early prediction of premature rupture of fetal membranes before delivery and formulation of corresponding intervention measures.

[PROBLEM TO BE SOLVED] To provide a method for producing ether phospholipids of high purity by an easy process. [SOLUTION] This production method comprises: (A) a step of extracting total lipids from organisms with non-polar organic solvent and branched alcohol mixture; and (B) a step of reacting the total lipids obtained by the step (A) with phospholipase A1 to decompose mixed diacyl-glycerophospholipids. This makes it possible to produce plasmalogen-type glycerophospholipids or ether phospholipids of high purity by an easy process.

The invention discloses a preparation method of fruity oil-water compatible edible essence and a preparation device thereof. The preparation raw materials: vanilla, strawberries, pineapples, mangoes,lemons, apples, sweet osmanthus flowers, vanillin, limonene, glycerol, phospholipid, D-mannitol, polydextrose, maltitol and a mixture of distilled water and propylene glycol. The preparation process comprises the following steps: preparing fruity essence, preparing oil-water compatible edible essence and preparing fruity oil-water compatible edible essence. Wherein the preparation of the fruity essence comprises the steps of crushing and stirring, mixing and soaking, extracting vanilla, preparing osmanthus fragrans oil and mixing materials; the preparation of the oil-water compatible edible essence comprises the steps of stirring and mixing, primary emulsification and secondary emulsification; and the preparation of the fruity oil-water compatible edible essence comprises the steps of stirring, cooling and performing standing. The problem that edible essence in the prior art cannot have two characteristics of water quality and oil quality at the same time is solved.

Magnetite particles suitable for injection into the blood stream of patients having enhanced resistance against agglomeration and uptake by the RES of the liver and spleen. The particles essentially consist of an iron oxide core and a phosphoric acid mono alkyl or alkenyl ester or glycerophospholipid / surfactant three dimensional shell surrounding the core. The core and the monoester or a micellar glycerophospholipid form an urchin-like structure which is further interlaced or intertwined with a non-ionic surfactant to produce a protective three dimensional shell which renders particles almost undetectable by the macrophages. Particles prepared according to the invention are kept in the blood circulation for long periods and represent excellent long lasting blood pool agents. Key components in the shell are (a) a polybasic mineral-organic species such as glycero phosphatidic acid in micellar form and (b) a block copolymer having successive hydrophilic and hydrophobic segments.

The invention discloses a method utilizing column chromatography to prepare high-purity L-alpha glyceryl phosphoryl ethanolamine (L-alpha-GPE) and belongs to the technical field of deep processing of phospholipid. The method comprises adopting sodium methoxide to catalyze soybean powder phospholipid to react in an absolute methanol system so as to prepare mixed liquid containing L-alpha glyceryl phosphoryl ethanolamine, after adjusting the pH, enabling the mixed liquid to pass through a strong cation exchange resin (absolute alcohol phase equilibrium) chromatographic column and a strong anion exchange resin (water balance) chromatographic column which are both capable of being used in non-aqueous media, adopting specific desorption solution to carry out desorption, finally performing reduced pressure distillation to the desorption solution, and obtaining light yellow transparent sticky liquid which is an L-alpha glyceryl phosphoryl ethanolamine product. The product obtained by the column chromatography is high in purity and low in cost, L-alpha-Glycerophosphorylcholine with high purity can be separated simultaneously, and the product is simple in process and suitable for industrial production. The method expands application range of deep processing products of phospholipid.

This invention provides a process for producing a high-purity sphingomyelin and a high-purity plasmalogen-form glycerophospholipid from a biological material by simple procedures at high yields. The process comprises the steps of: (A) subjecting dried total lipids extracted from a biological material to extraction treatment with a specific mixture solution to separate an insoluble portion composed mainly of sphingomyelin and a soluble portion; (B) subjecting the insoluble portion, obtained in said Step (A), to washing treatment with a specific mixture solution to obtain crude sphingomyelin; (C) subjecting the soluble portion, obtained in said Step (A), to washing treatment with a water-soluble ketone solvent to obtain crude plasmalogen-form glycerophospholipid; (D) causing an enzyme to act on the crude sphingomyelin, obtained in said Step (B), to obtain sphingomyelin having a purity of 90% or more; and (E) causing an enzyme to act on the crude plasmalogen-form glycerophospholipid, obtained in said Step (C), to obtain plasmalogen-form glycerophospholipid having a purity of 40% or more.

The present invention relates to an albumin nano particle composition and a preparation method thereof. The composition contains 100 parts by weight of the compound of formula (I) or a pharmaceutically acceptable salt thereof and 100-1500 parts by weight of albumin. The albumin is an albumin selected from the following: ovalbumin, bovine blood albumin, human blood albumin, recombinant human albumin, etc., preferably human blood albumin or recombinant human albumin; Optionally contain one or more excipients selected from the following: cholesterol, phospholipids, lecithin, PEGylated phospholipids, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, glycerophospholipids Acids, synthetic phospholipids and other types of phospholipids. The preparation method and pharmaceutical use of the composition are also provided, and the method of the invention presents excellent technical effects as described in the description.

The invention provides a compound comprising one, two, three or more non-natural HRC sequence of a viral spike peptide conjugated to a hydrophobic moiety via an optional linker. The hydrophobic moiety can be a membrane integrating ligand, such as a cholesterol, a sphingolipid, a glycolipid, a glycerophospholipid. The non-natural viral spike peptide is preferably a coronavirus spike protein characterized by one or more D-amino acids. The peptides of the invention inhibit viral fusion. The invention includes compositions for the delivery of compounds of the invention, such as pulmonary or nasal delivery. The invention also provides a method of treating or preventing a viral infection, including for example a SARS-CoV-2 (COVID-19) infection, in a subject in need thereof comprising administering an effective amount of a compound of the invention.

The invention relates to an enzymatic synthesis method of ferulic acid phosphoglyceride. The method is characterized by including adding an activated molecular sieve to an organic solvent for dehydration process for 24-96h to obtain the dehydrated organic solvent; enzymatic esterification reaction: adding hydrolyzed phospholipid and ferulic acid ethyl ester to the dehydrated organic solvent according to the mole ratio of 8:1 to 1:8, adding an enzyme and the activated molecular sieve, and shaking at a temperature of 40-70 DEG C for 10-240h; and filtering after reaction, performing rotary evaporation on the clarified filtrate to remove the organic solvent to obtain the ferulic acid phosphoglyceride with a conversion rate up to 40%. According to the method, the operation is simple, the method is environment-friendly, the catalyst and the enzyme can be recycled, and the obtained product is good in structural unicity.

Methods of treating an inert surface of a substrate to improve the adherence to the treated surface of micro-dimensioned particles including the steps of: contacting the inert surface with in an aqueous dispersion of a construct of the structure F-S-L; and then washing the surface with an aqueous vehicle to provide the treated surface, where F is a polyamine; S is —CO(CH2)2CO—, —CO(CH2)3CO—, —CO(CH2)4CO— or —CO(CH2)5CO—; and L is a diacyl- or dialkyl-glycerophospholipid.

The invention discloses a preparation method of glycerol phosphatidylcholine, wherein the preparation method comprises: 1) mixing the compound shown in formula (I) with anhydrous sodium carbonate to prepare the mixture M1; 2) mixing The mixture M1 and (R)-(-)-3-chloro-1,2-propanediol are placed in absolute ethanol and refluxed to obtain the mixture M2; 3) the mixture M2 is filtered and treated by membrane separation to remove the chlorination in the mixture M2 After adding sodium, the filtrate M3 is obtained; 4) adding zinc chloride to the filtrate M3 and then adding water to dissolve to obtain the mixture M4; 5) passing the mixture M4 through the ion exchange resin and then filtering the filtrate to obtain glycerol phosphatidylcholine; Wherein, R is Ca2+ or Mg2+. Through the above design, the preparation method of glycerol phosphatidylcholine is not only simple, but also the yield is high, thereby realizing the effect of simple preparation method, high synthesis rate and greatly reducing production cost.

The invention discloses a gene promoter region sequence of glycerophospholipid peptidy transeferace (LPAAT) and application in crop genetic improvement. The gene promoter region sequence of glycerophospholipid peptidy transeferace is shown as SEQ ID NO:1. The application method of the gene promoter of glycerophospholipid peptidy transeferace in crop genetic improvement comprises the following steps of: (1) inserting the gene promoter of glycerophospholipid peptidy transeferace into a promoter verifying expression vector pBI101.3 and starting the expression of a gus reporter gene; and (2) using the expression carrier for plant callus transgenosis research induced by agrobacterium tumefacien and obtaining the transgenosis plant by inducing, dip dyeing, coincubating and taking root of the plant callus. The gene promoter comes from coconut, can specially start expression of target gene in endosperm histiocyte, provides new promoter type in the future crop genetic improvement and has very high stability and operability without transgenic silencing.

A preparation for treatment and / or prevention of seizures comprising a non-mammalian derived mixture of serine glycerophospholipids (PS) conjugates, wherein the mixture comprises (a) Eicosapentaenoic acid (EPA) conjugated to PS and (b) Docosahexaenoic acid (DHA) conjugated to PS, and methods of treatment of seizures with same.

The invention belongs to the field of pesticide adjuvants, and provides a modified liposome, a composition, a preparation method and an application. The modified liposome is put into the reaction kettle under the action of a catalyst, and the mixture of glycerophospholipids, sterols and polyhydroxy compounds is used as a starter, and the temperature is raised for dehydration, and then ethylene oxide is passed through to carry out ring-opening polymerization and aging. , neutralized, and the polyhydroxy compound is a combination of alcohols and alcohol amines. The present invention also provides a liposome composition prepared from modified liposomes, which has the advantages of easy dispersion in water, good vesicle stability, easy biodegradation, and stable properties when used as a pesticide adjuvant. The advantages of good conduction effect and the ability to facilitate the deposition of liquid medicine on the target can be widely used in the field of pesticides.

The invention discloses an enzymatic method for preparing glycerol phosphatidylcholine, which belongs to the technical field of oil processing. After the enzyme is in contact with the substrate, the ratio of sn2-lysophospholipid and sn1-lysophospholipid in the hydrolyzate is regulated by heat treatment, and the conversion of sn2-lysophospholipid to sn1-lysophospholipid is promoted, thereby promoting the generation of glycerol phosphatidylcholine, greatly The production rate of glycerol phosphatidylcholine is improved; in addition, there is no need to add any metal ions as coenzyme factors during the reaction process, and the subsequent separation and purification steps are simple. The method is simple to operate, can significantly increase the production rate of glycerol phosphatidylcholine, and does not need to add metal ions in the reaction process, and the subsequent separation and purification steps are simple, and has good economic benefits and industrial application prospects.

The invention relates to the field of food processing, and particularly discloses a dried crayfish nut stick as well as a preparation method and application thereof. The dried crayfish nut bar comprises the following components: dried crayfish, nuts, cereal particles, slurry, calcium caseinate, soybean protein isolate, soybean protein peptide, composite vitamins and composite minerals. The slurry comprises the following components: trehalose, refined high oleic acid sunflower seed oil, isomaltooligosaccharide syrup, resistant paste liquid, glycerol, phospholipid and table salt; the water content of the dried crayfish is 10-14%, and the water activity is 0.6-0.7; the preparation method comprises the following steps: S1, preliminary mixing; S2, boiling the slurry; and S3, mixing and forming. The dried crayfish nut stick is balanced in nutrition, meets the requirements of the market for crayfish products, does not need to be vacuumized, has the shelf life as long as 12 months, is not easy to harden, has the characteristics of low calorie, high protein and high dietary fiber, tastes mellow, and is a healthy meal replacement snack.

The invention discloses a method for preparing glycerol phosphatidylcholine by an enzyme method and belongs to the technical field of grease processing. After an enzyme is in contact with a substrate,a ratio of sn2-lysophospholipid to sn1-lysophospholipid in a hydrolysate is regulated and controlled through heat treatment, a conversion from the sn2-lysophospholipid to the sn1-lysophospholipid ispromoted, then generation of the glycerol phosphatidylcholine is promoted, and a generation rate of the glycerol phosphatidylcholine is greatly increased. In addition, no metal ion is added as a coenzyme factor in a reaction process and subsequent separation and purification steps are simple. The method is simple to operate and can remarkably increase the generation rate of the glycerol phosphatidylcholine, metal ions do not need to be added in the reaction process, the subsequent separation and purification steps are simple, and the method has good economic benefits and industrial applicationprospects.

The invention discloses application of cold atmospheric plasma (CAP)-activated saline in the aspects of cell metabolism regulation and tumor treatment and a medicine. The saline is activated by using a cold atmospheric pressure plasmas technology, the activated saline acts on an in-vivo tumor, and the research conclusion comprises that active particles such as ROS and RNS of the CAP-activated saline cause change of the metabolic level of tumor cells (especially glycerophospholipid metabolic regulation), the in-vivo tumor can be ablated, and no toxic or side effect exists basically. Metabolite capable of generating up-regulation through metabolic regulation of glycerophospholipid is 3-acetyl-2, 5-dimethylthiophene and DL-glutamic acid, and metabolite capable of generating down-regulation is 5-methylcytidine, N2, N2-dimethyl guanosine, L-histidine and 1-methyl guanosine. Therefore, glycerophospholipid metabolism regulation drugs, tumor treatment drugs and the like can be prepared, and drug units can be packaged at proper doses.

The invention belongs to the technical field of biological sample materials and analysis, and discloses a detection method for high-abundance and low-abundance class phospholipid compounds in cells. According to the method, the UPLC-QqQ MS technology is adopted, and the phospholipid components in the cells are rapidly and effectively separated through UPLC; the glycerophosphatide components in cells are analyzed in a multi-reaction monitoring mode by adopting an ESI positive and negative ion scanning mode, and 218 phospholipid compounds are detected in total, including 44 PC and LPC; 37 kinds of PE and LPE; 38 kinds of PI and LPI; 24 kinds of PG; and 27 kinds of PA and LPA. The method has the characteristics of high specificity and high sensitivity, can be used as an effective means for separation and analysis of complex biological systems such as cells, serum and the like, and lays a foundation for research of phospholipid metabonomics.

A method for preparing industrialized egg yolk reaction drying medium, which comprises the following steps: extract the effective ingredients of egg yolk with absolute ethanol and acetone or use other glycerophospholipids, neutral fats, TritonX surfactants, and organic amine derivatives cationic surface Active agent or polyethylene glycol surfactant, mixed with metal cations, adding sodium chloride aqueous solution to grind, make it emulsified evenly, and prepare yolk emulsion suspension; then weigh the basal medium or preparation A with yolk reaction Mix with egg yolk suspension, adjust the pH value of the medium, and then dry, pulverize, sieve, and subpackage to prepare various egg yolk reaction dry culture medium. The method makes the industrialized production of the yolk reaction dry medium possible, the preparation is simple, the experimental process is simplified, the use is convenient, the cost is low, and the error of the experimental result is small, so the application field of the yolk reaction medium is greatly expanded.

The present invention provides a pharmaceutical composition for the lubrication of joints, the pharmaceutical composition comprising a non-ionic tonicity agent comprising a polyol, and liposomes comprising at least one membrane comprising at least one phospholipid (PL) selected from a glycerophospholipid (GPL), said GPL having two C12-C18 hydrocarbon chains, being the same or different, and sphingomyelin (SM) having a C12-C18 hydrocarbon chain, the pharmaceutical composition being essentially free of an additional pharmaceutically active agent, wherein the at least one membrane has a phase transition temperature in the range of about 20° C. to about 39° C. and the joint has a joint temperature which is above the phase transition temperature.