38 results about "Glycerophospholipid" patented technology

The invention discloses recombinant escherichia coli, a method for preparing phospholipase C and application. The recombinant escherichia coli BL21(DE-3)-pET-28a-L.m-phospholipase C (PLC) of which the target gene is from listeria monocyogenes (L.monocytogenes) is utilized as a fermentation strain to carry out liquid fermentation, so as to prepare phospholipase C. The method disclosed by the invention is simple in enzyme production process, short in production cycle and low in cost. A glycerophosphate bond on glyceryl phosphatide C3 can be specifically hydrolyzed to produce diglyceride (DAG) by using recombinant phospholipase C; plant crude oil (soybean oil, colleseed oil, rice bran oil and the like) is degummed by phospholipase C.

The invention belongs to the field of pesticide aids, and provides a modified liposome and a composition, and preparation methods and applications of the modified liposome and the composition. The preparation method of the modified liposome comprises: adding a mixture of glycerophospholipid, sterol and a polyhydroxy compound into a reaction kettle as an initiator under the action of a catalyst, carrying out heating dehydration, introducing ethylene oxide, and carrying out ring-opening polymerization, aging and neutralization to prepare the product, wherein the polyhydroxy compound is a composition of alcohol and alcohol amine. The invention further provides a liposome composition prepared from the modified liposome, wherein the liposome composition as a pesticide aid has advantages of easydispersion in water, good vesicle stability, easy biodegradation, stable properties, good conduction effect on targets and easy deposition of pesticide liquids on targets, and can be widely used in the field of pesticides.

The invention discloses a melt-in-the-mouth cereal bar and a preparation method thereof. According to the main point of the technical scheme, the melt-in-the-mouth cereal bar comprises the follow components: puffed purple rice, puffed white rice, puffed yellow rice, chia seeds, protein powder, sugar powder, coconut milk powder, oat powder, yoghurt powder, high fructose corn syrup, isomaltooligosaccharide syrup, resistant dextrin liquid, glycerol, phospholipid and sunflower seed oil. By using puffed purple rice, puffed white rice and puffed yellow rice as main raw materials, adjusting the dosage of puffed particles and cooperating with coconut milk powder, yogurt powder, protein powder and chia seeds, the cereal bar contains rich protein and amino acids, and the energy of the cereal bar isincreased; moreover, the prepared cereal bar has the melt-in-the-mouth taste, and is especially suitable for children to eat.

The invention discloses a method for preparing glycerol phosphatidylcholine from soybean powder phospholipid, wherein the method comprises the following steps of: 1) mixing methanol and ethanol to prepare mixture M1; 2) adding soybean powder phospholipid into the mixture M1 to prepare a mixture M2; 3) adding sodium methoxide into the mixture M2 to conduct mixed action at a temperature of 30 to 50DEG C to prepare a reaction liquid M3; 4) after adding ethanol into the reaction solution M3, removing the ethanol layer to obtain the crude extract M3; 5) exchanging resin column of the crude extractM3 sequentially with a silica gel column and an ion exchange, evaporating and concentrating to obtain glycerol phosphatidylcholine. The soybean powder phospholipid used as a raw material is realized,and the yield effect of the soybean powder phospholipid can be improved.

The invention discloses a biomarker and a method for predicting premature rupture of fetal membranes, and the biomarker is selected from one or more of 4-methylvaleraldehyde (isohexanal), sphingolipids (SP) metabolite, glycerolipids (GL) metabolite and glycerophospholipids (GP) metabolite; the biomarker has a change in content difference multiple between a pregnant female body and a healthy pregnant female body at risk of premature rupture of fetal membranes. The biomarker for predicting premature rupture of fetal membranes and the prediction method have important significance for early prediction of premature rupture of fetal membranes before delivery and formulation of corresponding intervention measures.

Magnetic iron oxide particles with enhanced anti-aggregation and anti-hepatosplenic RES uptake suitable for infusion into the patient's bloodstream, comprising an iron oxide core and mono- or alkenyl phosphate or glycerophospholipid/surfactant surrounding the core agent three-dimensional shell. The core core and monoester or colloidal glycerophospholipids form a hedgehog-like structure, and then interweave or coil with nonionic surfactants to form a protective three-dimensional shell. The granule can exist in the blood circulation for a long time and is an excellent long-acting blood pool reagent.

The invention discloses a method utilizing column chromatography to prepare high-purity L-alpha glyceryl phosphoryl ethanolamine (L-alpha-GPE) and belongs to the technical field of deep processing of phospholipid. The method comprises adopting sodium methoxide to catalyze soybean powder phospholipid to react in an absolute methanol system so as to prepare mixed liquid containing L-alpha glyceryl phosphoryl ethanolamine, after adjusting the pH, enabling the mixed liquid to pass through a strong cation exchange resin (absolute alcohol phase equilibrium) chromatographic column and a strong anion exchange resin (water balance) chromatographic column which are both capable of being used in non-aqueous media, adopting specific desorption solution to carry out desorption, finally performing reduced pressure distillation to the desorption solution, and obtaining light yellow transparent sticky liquid which is an L-alpha glyceryl phosphoryl ethanolamine product. The product obtained by the column chromatography is high in purity and low in cost, L-alpha-Glycerophosphorylcholine with high purity can be separated simultaneously, and the product is simple in process and suitable for industrial production. The method expands application range of deep processing products of phospholipid.

The invention provides a compound comprising one, two, three or more non-natural HRC sequence of a viral spike peptide conjugated to a hydrophobic moiety via an optional linker. The hydrophobic moiety can be a membrane integrating ligand, such as a cholesterol, a sphingolipid, a glycolipid, a glycerophospholipid. The non-natural viral spike peptide is preferably a coronavirus spike protein characterized by one or more D-amino acids. The peptides of the invention inhibit viral fusion. The invention includes compositions for the delivery of compounds of the invention, such as pulmonary or nasal delivery. The invention also provides a method of treating or preventing a viral infection, including for example a SARS-CoV-2 (COVID-19) infection, in a subject in need thereof comprising administering an effective amount of a compound of the invention.

The invention relates to the field of food processing, and particularly discloses a dried crayfish nut stick as well as a preparation method and application thereof. The dried crayfish nut bar comprises the following components: dried crayfish, nuts, cereal particles, slurry, calcium caseinate, soybean protein isolate, soybean protein peptide, composite vitamins and composite minerals. The slurry comprises the following components: trehalose, refined high oleic acid sunflower seed oil, isomaltooligosaccharide syrup, resistant paste liquid, glycerol, phospholipid and table salt; the water content of the dried crayfish is 10-14%, and the water activity is 0.6-0.7; the preparation method comprises the following steps: S1, preliminary mixing; S2, boiling the slurry; and S3, mixing and forming. The dried crayfish nut stick is balanced in nutrition, meets the requirements of the market for crayfish products, does not need to be vacuumized, has the shelf life as long as 12 months, is not easy to harden, has the characteristics of low calorie, high protein and high dietary fiber, tastes mellow, and is a healthy meal replacement snack.

The invention discloses application of cold atmospheric plasma (CAP)-activated saline in the aspects of cell metabolism regulation and tumor treatment and a medicine. The saline is activated by using a cold atmospheric pressure plasmas technology, the activated saline acts on an in-vivo tumor, and the research conclusion comprises that active particles such as ROS and RNS of the CAP-activated saline cause change of the metabolic level of tumor cells (especially glycerophospholipid metabolic regulation), the in-vivo tumor can be ablated, and no toxic or side effect exists basically. Metabolite capable of generating up-regulation through metabolic regulation of glycerophospholipid is 3-acetyl-2, 5-dimethylthiophene and DL-glutamic acid, and metabolite capable of generating down-regulation is 5-methylcytidine, N2, N2-dimethyl guanosine, L-histidine and 1-methyl guanosine. Therefore, glycerophospholipid metabolism regulation drugs, tumor treatment drugs and the like can be prepared, and drug units can be packaged at proper doses.

The invention belongs to the technical field of biological sample materials and analysis, and discloses a detection method for high-abundance and low-abundance class phospholipid compounds in cells. According to the method, the UPLC-QqQ MS technology is adopted, and the phospholipid components in the cells are rapidly and effectively separated through UPLC; the glycerophosphatide components in cells are analyzed in a multi-reaction monitoring mode by adopting an ESI positive and negative ion scanning mode, and 218 phospholipid compounds are detected in total, including 44 PC and LPC; 37 kinds of PE and LPE; 38 kinds of PI and LPI; 24 kinds of PG; and 27 kinds of PA and LPA. The method has the characteristics of high specificity and high sensitivity, can be used as an effective means for separation and analysis of complex biological systems such as cells, serum and the like, and lays a foundation for research of phospholipid metabonomics.

The present invention provides a pharmaceutical composition for the lubrication of joints, the pharmaceutical composition comprising a non-ionic tonicity agent comprising a polyol, and liposomes comprising at least one membrane comprising at least one phospholipid (PL) selected from a glycerophospholipid (GPL), said GPL having two C12-C18 hydrocarbon chains, being the same or different, and sphingomyelin (SM) having a C12-C18 hydrocarbon chain, the pharmaceutical composition being essentially free of an additional pharmaceutically active agent, wherein the at least one membrane has a phase transition temperature in the range of about 20° C. to about 39° C. and the joint has a joint temperature which is above the phase transition temperature.