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Method for preparing glycerol phosphatidylcholine from soybean powder phospholipid

A technology of glycerol phosphatidylcholine and soybean powder phospholipid, which is applied in the direction of edible phospholipid composition, chemical instrument and method, application, etc., and can solve the problem of low yield

Inactive Publication Date: 2019-01-04
WUHU FOMAN BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The conventional method for preparing glycerol phosphatidylcholine is often a synthetic method, and the commonly used soybean phospholipids to prepare glycerol phosphatidylcholine often have a low yield after preparation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Mix 1 volume part of methanol and 2 volume parts of ethanol to prepare a mixed solution M1;

[0039] 2) Add 10 g of soybean powder phospholipids to 20 mL of the mixed liquid M1 to prepare the mixture M2;

[0040] 3) At a temperature of 30°C, add 1 mL of sodium methoxide to 100 mL of the mixture M2, and mix and react for 2 hours under stirring conditions to obtain a reaction solution M3;

[0041] 4) Add 15 mL of ethanol to 10 mL of the above-mentioned reaction solution M3, mix and remove the ethanol layer to obtain a crude extract M3;

[0042] 5) The crude extract M3 is passed through a silica gel column and an ion exchange resin column in sequence, and then placed in a thin-film evaporator for evaporation and concentration to obtain glycerol phosphatidylcholine A1. (yield is 81.2%, purity is 91.5%)

Embodiment 2

[0044] 1) Mix 1 volume part of methanol and 5 volume parts of ethanol to prepare a mixed liquid M1;

[0045] 2) Add 10 g of soybean powder phospholipids to 30 mL of the mixed solution M1 to prepare the mixture M2;

[0046] 3) At a temperature of 50°C, add 5 mL of sodium methoxide to 100 mL of the mixture M2, and mix and react for 3 hours under stirring conditions to obtain a reaction solution M3;

[0047] 4) Add 20 mL of ethanol to 10 mL of the above-mentioned reaction solution M3, mix and remove the ethanol layer to obtain a crude extract M3;

[0048] 5) Take the crude extract M3 and pass it through a silica gel column and an ion exchange resin column in sequence, and place it in a thin-film evaporator for evaporation and concentration to obtain glycerol phosphatidylcholine A2. (yield is 82.1%, purity is 91.6%)

Embodiment 3

[0050] 1) Mix 1 volume part of methanol and 3 volume parts of ethanol to prepare a mixed liquid M1;

[0051] 2) Add 10 g of soybean powder phospholipids to 25 mL of the mixed solution M1 to prepare the mixture M2;

[0052] 3) At a temperature of 40°C, add 3 mL of sodium methoxide to 100 mL of the mixture M2 and mix and react for 3 hours under stirring to obtain the reaction solution M3;

[0053] 4) Add 18mL of ethanol to 10mL of the above reaction solution M3, mix and remove the ethanol layer to obtain the crude extract M3;

[0054] 5) The crude extract M3 is passed through a silica gel column and an ion exchange resin column in sequence, and then placed in a thin film evaporator for evaporation and concentration to obtain glycerol phosphatidylcholine A3. (yield is 83.5%, purity is 93.6%)

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Abstract

The invention discloses a method for preparing glycerol phosphatidylcholine from soybean powder phospholipid, wherein the method comprises the following steps of: 1) mixing methanol and ethanol to prepare mixture M1; 2) adding soybean powder phospholipid into the mixture M1 to prepare a mixture M2; 3) adding sodium methoxide into the mixture M2 to conduct mixed action at a temperature of 30 to 50DEG C to prepare a reaction liquid M3; 4) after adding ethanol into the reaction solution M3, removing the ethanol layer to obtain the crude extract M3; 5) exchanging resin column of the crude extractM3 sequentially with a silica gel column and an ion exchange, evaporating and concentrating to obtain glycerol phosphatidylcholine. The soybean powder phospholipid used as a raw material is realized,and the yield effect of the soybean powder phospholipid can be improved.

Description

technical field [0001] The invention relates to the field of preparation of glycerol phosphatidylcholine, in particular to a method for preparing glycerol phosphatidylcholine by using soybean powder phospholipids. Background technique [0002] Glycerol phosphatidylcholine is the product of two fatty acyl groups on the phosphatidylcholine (PC) molecule being completely hydrolyzed. It has a significant effect on stabilizing the cognitive ability of the brain, and can even repair some of the early senile dementia patients. Impaired cognitive abilities. Unlike drugs that inhibit the breakdown of acetylcholine, GPC stimulates the body to produce new phosphatidylcholine. Animal studies have shown that GPC can prevent memory loss. Compared with a control group of experimental conditions, it was found that older mice fed GPC mice had less loss of neural connections in the hippocampus, an area of ​​the brain dedicated to memory and learning. Based on a review of 13 clinical trial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 张慈铭
Owner WUHU FOMAN BIOPHARMA CO LTD
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