Enzymatic synthesis method of ferulic acid phosphoglyceride

A ferulic acid glycerophospholipid, enzymatic synthesis technology, applied in the direction of fermentation, etc., can solve the problems of poor solubility of ferulic acid, low activity of ferulic acid, etc., and achieve the effect of environmental friendliness, simple operation and good product structure

Inactive Publication Date: 2013-02-27
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor solubility of ferulic acid in both hydrophobic and hydrophilic environments, the low activity of ferulic acid

Method used

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  • Enzymatic synthesis method of ferulic acid phosphoglyceride

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The synthesis of ferulic acid glycerophospholipids catalyzed by different lipases, the specific steps are as follows:

[0020] (1) will After the molecular sieve is activated, it is added to the reaction solvent to be used, and the solvent is dried. After shaking overnight for 24 hours, a dehydrated organic solvent is obtained. The dosage of the molecular sieve is 200 mg / mL.

[0021] (2) Enzymatic transesterification reaction: Add ethyl ferulate and phosphatidylcholine to several milliliters of the above-mentioned dehydrated organic solvent toluene according to the molar ratio of 3:1, and then add 40 mg / mL of various enzymes class, Novozym 435 (Denmark), Lipozyme RM IM (Denmark), Lipozyme TL IM (Denmark). Add molecular sieve 80mg / mL, shake at 200r / min for 96h at 55°C. After the reaction, samples were taken, molecular sieves and enzymes were removed by membrane filtration, and the obtained filtrate was dried with nitrogen to remove organic solvents, and then entered i...

Embodiment 2

[0023] Different dual organic solvents catalyze the enzymatic synthesis of ferulic acid glycerophospholipid, and the specific steps are as follows:

[0024] (1) will After the molecular sieve is activated, it is added to various reaction solvents to be used, and the solvent is dried. After shaking overnight for 24 hours, a dehydrated organic solvent is obtained. The dosage of the molecular sieve is 200 mg / mL.

[0025] (2) Enzymatic transesterification reaction: Add ethyl ferulate and phosphatidylcholine to the above-mentioned dehydrated organic solvent at a mass molar ratio of 3:1 for ultrasonic dissolution, which includes toluene / n-butanol (90:10, v / v), toluene / chloroform (90:10, v / v), toluene / isopropanol (90:10, v / v), toluene / tert-butanol (90:10, v / v), Toluene / acetone (90:10, v / v) and toluene / DMSO (90:10, v / v). Lipase Novozym 435 was added at 40 mg / mL. Add molecular sieve 80mg / mL, shake at 200r / min for 96h at 55°C. After the reaction, samples were taken, molecular sieve...

Embodiment 3

[0027] The synthesis of the ferulic acid glycerophospholipid of different substrate molar ratios, concrete steps are as follows:

[0028] (1) will After the molecular sieve is activated, it is added to the reaction solvent to be used, and the solvent is dried. After shaking overnight for 24 hours, a dehydrated organic solvent is obtained. The dosage of the molecular sieve is 200 mg / mL.

[0029] (2) Enzymatic transesterification reaction: Ethyl ferulate and phosphatidylcholine were mixed in molar ratios of 8:1, 6:1, 5:1, 3:1, 2:1 and 1:1, respectively. After adding to several milliliters of the above-mentioned dehydration organic solvent toluene / chloroform (90:10, v / v) for ultrasonic dissolution, add 40 mg / mL of various enzymes, Novozym 435 (Denmark), add molecular sieves 80 mg / mL, in Shake at 200r / min for 96h at 55°C. After the reaction, samples were taken, molecular sieves and enzymes were removed by membrane filtration, and the obtained filtrate was dried with nitrogen to...

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Abstract

The invention relates to an enzymatic synthesis method of ferulic acid phosphoglyceride. The method is characterized by including adding an activated molecular sieve to an organic solvent for dehydration process for 24-96h to obtain the dehydrated organic solvent; enzymatic esterification reaction: adding hydrolyzed phospholipid and ferulic acid ethyl ester to the dehydrated organic solvent according to the mole ratio of 8:1 to 1:8, adding an enzyme and the activated molecular sieve, and shaking at a temperature of 40-70 DEG C for 10-240h; and filtering after reaction, performing rotary evaporation on the clarified filtrate to remove the organic solvent to obtain the ferulic acid phosphoglyceride with a conversion rate up to 40%. According to the method, the operation is simple, the method is environment-friendly, the catalyst and the enzyme can be recycled, and the obtained product is good in structural unicity.

Description

technical field [0001] The invention belongs to the field of preparation of ferulic acid glycerophospholipid, and relates to a method for enzymatically synthesizing ferulic acid glycerophospholipid. Background technique [0002] The scientific name of ferulic acid is 4-hydroxy-3-methoxycinnamic acid, also known as 4-hydroxy-3-methoxy-2-phenylacrylic acid, which is a substance rich in the plant kingdom. It is used in food, cosmetics, medicine It is used as antioxidant, ultraviolet absorber, antibacterial and anti-inflammatory, anticancer, antimutagenic and other ingredients. However, due to the poor solubility of ferulic acid in both hydrophobic and hydrophilic environments, the low activity of ferulic acid eventually resulted. [0003] The ferulic acid glycerophospholipid has a good antioxidant effect, and the ferulic acid glycerophospholipid greatly improves the water solubility of ferulic acid, which is beneficial to its application in a hydrophilic environment and increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P9/00
Inventor 杨天奎杨宏黎牟英
Owner DALIAN UNIV OF TECH
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