A method for enzymatically preparing glycerol phosphatidylcholine

A technology for glycerol phosphatidylcholine and enzymatic preparation, which is applied in the field of oil processing, can solve the problems of shortening reaction time, long reaction time, and increasing the difficulty of separation and purification, and achieves simple separation and purification steps, increased difficulty, and increased production rate Effect

Active Publication Date: 2022-03-11
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conclusion, the yield of glycerol phosphatidylcholine is higher when enzymatic hydrolysis is used to prepare glycerol phosphatidylcholine, but the reaction time is longer; although by changing the reaction system and adding Ca 2+ It can significantly increase the rate of hydrolysis reaction and greatly shorten the reaction time, but it increases the difficulty of subsequent separation and purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Accurately weigh a certain amount of lecithin and mix it with deionized water (a total of 10Kg, the concentration of lecithin in deionized water is 30mg / mL), stir at a speed of 400rpm for 20min to emulsify, the reaction mixture is transported to the packed bed through a material pump The reactor is reacted, and the filling enzyme is immobilized phospholipase A 1 (Using physical adsorption method or covalent binding method, according to phospholipase A 1 The ratio to the immobilized carrier is 20-80mg / g resin, stirred at 50-200rpm at 30°C for 1-8h), the filling amount is 0.5Kg, and the reaction temperature is 55°C. Then the reaction material enters the heat treatment device and stays at 60°C for 40 minutes. After the heat-treated reaction material is cooled to 55°C, it is pumped into the material tank again for circular reaction. Adjust the flow rate of the reaction substrate so that each cycle is mixed with immobilized phospholipase A 1 The contact reaction time is 20...

Embodiment 2

[0032] Accurately weigh a certain amount of lecithin and mix it with deionized water (a total of 10Kg, the concentration of lecithin in deionized water is 55mg / mL), after stirring at a speed of 600rpm for 15min to emulsify, the reaction mixture is transported to the packed bed through a material pump The reactor is reacted, and the filling enzyme is immobilized phospholipase A 1 (Prepare immobilized phospholipase A by the method described in the present invention 1), the filling amount is 2Kg, and the reaction temperature is 50°C. Then the reaction material enters the heat treatment device and stays at 70°C for 20 minutes. After the heat-treated reaction material is cooled to 50°C, it is pumped into the material tank again for circular reaction. Adjust the flow rate of the reaction substrate so that each cycle is mixed with immobilized phospholipase A 1 The contact reaction time is 10min. After 3 hours of cyclic reaction, samples were taken to measure the composition of the...

Embodiment 3

[0034] Accurately weigh a certain amount of lecithin and mix it with deionized water (a total of 10Kg, the concentration of lecithin in deionized water is 45mg / mL), after stirring at a speed of 400rpm for 20min to emulsify, the reaction mixture is transported to the packed bed through a material pump The reactor is reacted, and the filling enzyme is immobilized phospholipase A 1 (Prepare immobilized phospholipase A by the method described in the present invention 1 ), the filling amount is 1.5Kg, and the reaction temperature is 35°C. Then the reaction material enters the heat treatment device and stays at 65°C for 30 minutes. After the heat-treated reaction material is cooled to 35°C, it is pumped into the material tank again for circular reaction. Adjust the flow rate of the reaction substrate so that each cycle is mixed with immobilized phospholipase A 1 The contact reaction time is 15min. After 3 hours of cyclic reaction, samples were taken to measure the composition of ...

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Abstract

The invention discloses an enzymatic method for preparing glycerol phosphatidylcholine, which belongs to the technical field of oil processing. After the enzyme is in contact with the substrate, the ratio of sn2-lysophospholipid and sn1-lysophospholipid in the hydrolyzate is regulated by heat treatment, and the conversion of sn2-lysophospholipid to sn1-lysophospholipid is promoted, thereby promoting the generation of glycerol phosphatidylcholine, greatly The production rate of glycerol phosphatidylcholine is improved; in addition, there is no need to add any metal ions as coenzyme factors during the reaction process, and the subsequent separation and purification steps are simple. The method is simple to operate, can significantly increase the production rate of glycerol phosphatidylcholine, and does not need to add metal ions in the reaction process, and the subsequent separation and purification steps are simple, and has good economic benefits and industrial application prospects.

Description

technical field [0001] The invention belongs to the technical field of oil processing, in particular to a method for enzymatically preparing glycerol phosphatidylcholine. Background technique [0002] Glycerol phosphatidylcholine (L-alpha glycerylphosphorylcholine, L-α-GPC) is the choline source for the synthesis of acetylcholine and phosphatidylcholine in the body. Previous studies have shown that glycerol phosphatidylcholine has important medicinal value in the treatment of Alzheimer's disease, cerebellar ataxia, schizophrenia and bipolar disorder, but as a medicinal raw material and related research Therefore, higher purity glycerol phosphatidylcholine is required. However, there is less glycerol phosphatidylcholine from natural sources, so it is of great significance to research and develop the preparation process of high-purity glycerol phosphatidylcholine. [0003] At present, the commonly used methods for preparing glycerol phosphatidylcholine mainly include enzymat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C07F9/09
CPCC12P13/001C12P7/6481C07F9/091
Inventor 李道明张佳浩周端王盼雪刘宁
Owner SHAANXI UNIV OF SCI & TECH
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