2results about How to "Simplify separation and purification steps" patented technology

The present application relates to the technical field of synthetic biology, in particular to a novel taxane acetyl hydrolase, nucleic acid molecule, biological material and application thereof. The acetyl hydrolase comprises at least one of the following sequences: the amino acid sequence of the enzyme shown in SEQ ID NO. 1, SEQ ID NO. 9, SEQ ID NO. 16, SEQ ID NO. 19, SEQ ID NO. 21, SEQ ID NO. 24 or a protein with the same function obtained by substitution and / or deletion and / or addition. The above-mentioned biological enzyme has stronger site selectivity and more controllable product spectrum, has clear regional selectivity for taxane acetyl hydrolysis, can reduce excessive hydrolysis and by-products caused by chemical hydrolysis or non-specific esterase, and has important value for green manufacturing application of taxane.

The application provides a method for preparing fish skin collagen peptides with high antioxidant activity by DHPM and UIO-66 molecular imprinting, and relates to the technical fields of aquatic product deep processing and bioactive peptides. The method comprises the following steps: fish skin pretreatment, DHPM-induced collagen protein unfolding, endogenous enzyme autolysis, crude separation, selective target enrichment by using UIO-66 material with histidine as a molecular imprinting template, and drying collection. The application destroys the dense triple helix structure of collagen protein by using the physical field effect of dynamic high pressure microfluidization, exposes the enzyme cutting site, uses the endogenous enzyme of fish skin for autolysis, and then uses the histidine molecular imprinting UIO-66 material to accurately capture the high antioxidant activity peptide segment, so that the enzymolysis efficiency and the antioxidant activity of the product are significantly improved. The application does not need to add exogenous enzymes, and has the advantages of green process, low cost and high product purity. The obtained fish skin collagen peptides can be widely applied to the fields of functional food, cosmetics and medicine.