38results about How to "Mild culture conditions" patented technology

The invention discloses a bacillus sphaericus with high yield and high-temperature xylanase resistance and an application thereof. The bacillus sphaericus is classified and named as Lysinibacillus sphaericus Xyn-1, and is collected in China Center for Type Culture Collection (CCTCC) on May, 5th, 2014 with the collection number CCTCCM2014183. The optimal fermentation enzyme production condition of a strain screening shake flask is determined by adopting a Plackett-Burman design and a response surface analysis method, and the fermentation level under the condition can reach 5678.5IU/mL. When the enzyme produced by fermentation of the strain is applied to degradation of plant hemicellulose, the xylobiose yield of the hemicellulose can reach over 60% through enzymolysis.

The invention discloses a preparation method of a squalene synthetase inhibitor CJ-13982. The compound CJ-13982 is separated and prepared from a fermentation culture of nigrospora oryzae A8 which has a collection number of CCTCC NO. M 2011412. The invention provides a method for preparing the compound CJ-13982 with the squalene synthetase inhibiting effect from the fermentation culture of a plant endophytic fungi nigrospora oryzae A8. The method is advantaged in mild culturing condition, simple and feasible culturing medium preparation, simple separation and purification steps, and high yield. With the method, large-scale production can be realized in a manual controlling condition. With the method, a problem of raw material required by compound CJ-13982 researches or applications can easily be solved, and a novel approach is provided for the preparation of the compound CJ-13982.

The invention discloses a biological transformation and synthesis method of rubescensin. The synthesis method comprises the following steps of: firstly, preparing a solid culture medium and a liquid culture medium; then, sterilizing and then inoculating the leaves, leaf buds or rhizome shucks of rabdosia rubescens into the prepared solid culture medium for culturing; transferring the callus obtained by culturing into the liquid culture medium for culturing; extracting the callus cultured in the liquid culture medium by adopting an organic solvent; and removing the organic solvent after extracting to obtain a rubescensin product (kaurene type diterpenoid compound). In the invention, the technical scheme for culturing and preparing rubescensin is free from the restriction of natural resources and is favorable for protecting the ecological environment. The technical scheme disclosed by the invention is easy to realize industrialized production, has the advantages of shorter culture time and higher product yield, lowers the production cost, and has obvious economic and social benefits.

The invention relates to the technical field of terpenes and provides a compound peniroquesine A with anti-inflammatory activity. The structure of the compound is shown as formula I in the description. The compound belongs to sesterterpenes and has remarkable anti-inflammatory activity. The compound peniroquesine A with the anti-inflammatory activity is sesterterpenes with novel 5/6/5/6/5 five-ring skeleton structure, and variety of the sesterterpenes is enriched. The invention further provides a preparation method of the compound peniroquesine A with anti-inflammatory activity. The compound peniroquesine A is prepared from Penicillium roqueforti by fermenting. The method is simple, fast, low in cost and suitable for large-scale industrial production.

The invention belongs to the technical field of fermentation engineering, in particular to a method for producing beta-galactosidase by liquid fermentation of Bacillus coagulans and the produced beta-galactosidase. Bacillus coagulans is specifically Bacillus coagulans (Bacillus coagulans) mutant BR-171, with the deposit number of CGMCC No. 14413. The strain is capable of producing beta-galactosidase stably and efficiently. The yield is more than 4000u/ml after submerged fermentation in a 50L fermentor and fed-batch fermentation for 84h. The beta-galactosidase produced by the strain has an optimum pH of 5.0, and still has an activity of more than 80% in the range of beta-galactosidase pH 2.5-8.5. The pH tolerance of galactosidase is suitable for most dairy products. The optimum temperatureof beta-galactosidase is 60 DEG C, and the activity of beta-galactosidase still reaches 88.4% after incubation at 65 DEG C for 2 h, which indicated that beta-galactosidase had significant heat resistance. It has high industrial value in dairy industry.

The invention discloses laccase-producing Coriolopsis gallica NCULAC F1. The laccase-producing Coriolopsis gallica is preserved in China Center for Type Culture Collection on June 17, 2021, the preservation number of the laccase-producing Coriolopsis gallica is CCTCC No.M2021731, and the preservation address of the laccase-producing Coriolopsis gallica is No.299, Bayi Road, Wuchang District, Wuhan, Hubei Province. The Coriolopsis gallica NCULAC F1 disclosed by the invention has the following characteristics that the laccase-producing activity is high, and produced laccase has good thermal stability and has relatively high tolerance to metal ions, organic solvents and the like. The Coriolopsis gallica NCULAC F1 is used as an enzyme-producing strain, and a proper amount of copper sulfate is added into a PDA liquid culture medium, so that the yield of the extracellular laccase with high activity expressed by the Coriolopsis gallica is greatly improved. The method for preparing a high-activity laccase crude solution from the Coriolopsis gallica NCULAC F1 by adding the copper sulfate has the characteristics of simplicity and convenience in operation, simple process, good repeatability and the like, and the prepared laccase solution is relatively high in activity and has good application potential and development and utilization prospects in the industrial field.

The invention discloses a biological system for efficiently detecting sweetness strength of a to-be-detected matter by taking yeast cell as host on the basis of combining sweetness receptor protein T1R2/T1R3 with a detection gene path. The biological system comprises the sweetness receptor protein T1R2/T1R3 subjected to modification and codon optimization, a modified Gpa1 protein adaptive to T1R2/T1R3, a G protein coupling signal path for signal amplification without signal interference and an inducible detection gene path with fluorescent protein or enzyme as a reporter gene. After the to-be-detected matter is added into a culture environment and the sweet matter molecule is combined with the sweetness receptor, the host G protein coupling signal path is utilized to amplify the signal and activate a downstream detection path, the expression condition of the report gene is taken as signal output, and the sweetness strength of the sweet matter is represented by the signal output strength. The biological system disclosed by the invention has the advantages of simple culture condition, low cost, short period, wide detection scope, high repeatability, high stability and capability of realizing the efficient detection for the sweetness strength of different sweet matters.

The invention belongs to the technical field of fermentation engineering, in particular to a Bacillus coagulans strain having a high beta-galactosidase yield and a liquid fermentation technique thereof. The strain is a Bacillus coagulans mutant strain BR-171 with an accession number of CGMCC No.14413. The strain can produce the beta-galactosidase stably in a high efficiency. Through deep fermentation in a fermentation tank having a volume of 50 L, and fermentation culture with material supplement for 84 h, the enzyme yield can be 4000 u/ml or above. The optimum pH of the beta-galactosidase produced by the strain is 5.0, and activity can still be 80% or more in a pH range of 2.5-8.5. The pH tolerance of the beta-galactosidase is suitable for most dairy products. The optimum temperature of the beta-galactosidase is 60 DEG C, and the residual activity of the beta-galactosidase can be 88.4 after incubation at 65 DEG C for 2 h, which indicates that the beta-galactosidase has significant heat resistance. The beta-galactosidase has high industrial value in dairy industry.

The invention provides bacterial strains for producing 5-hydroxyl-4-decalactone and application of the bacterial strains, belongs to the field of microorganisms, and discloses a bacterial strain capable of preparing 5-hydroxyl-4-decalactone by means of conversion, application of the bacterial strain to preparing the 5-hydroxyl-4-decalactone by means of microbial conversion and a method for applying the bacterial strain to preparing the 5-hydroxyl-4-decalactone. The bacterial strain can be used for efficiently preparing the 5-hydroxyl-4-decalactone by means of conversion by the aid of plant oil which is used as a substrate. The bacterial strain is preserved in the China Center for Type Culture Collection on May 19th, 2017, and a preservation number of the bacterial strain is CCTCC No.M2017274. The bacterial strain, the application and the method have the advantages that conditions for procedures for preparing the 5-hydroxyl-4-decalactone by means of conversion are mild, reaction can be quickly carried out, operation is simple, the method is safe as compared with existing chemical synthesis methods and the like, and environmental pollution can be prevented.

The invention relates to a method for separating and preparing compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) with the fermentation broth of Polyporus picipes. The method adopting a plate-to-bottle liquid culture method includes the following steps: a peeled potato, glucose and distilled water, the pH of which is about 7.2, are first sterilized and prepared into culture medium; the culture medium is then fermented into fermentation broth, the fermentation broth is extracted with ethyl acetate to obtain extract, and the extract is separated by column chromatography, and is eluted at a gradient by a chloroform-methanol system; and after separation by multiple times of normal-phase silica gel column chromatography, a reversed-phase C18 column, SephadexLH-20 gel column chromatography and recrystallization, the pure compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) is obtained. The invention can develop the compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) into the technical product of pathogenic fungus-resistant antibiotic, solves the problem of material resources for the 2,4-dyhydroxyl-5-methyl-acetophenone, and has the advantages of mild culture conditions, simple and easy culture medium preparation, short fermentation time and the like.

The invention relates to an application method for potash fertilizer. The application method for the potash fertilizer is characterized by comprising the following steps: sampling to-be-fertilized land so as to obtain a soil sample; preparing a liquid soil medium from the soil sample; culturing silicate bacteria with the liquid soil medium so as to obtain a culture solution; measuring the content of rapidly available potassium in the culture solution; and applying the potash fertilizer and silicate bacteria fertilizer according to the content of rapidly available potassium and demands of the to-be-fertilized land for potassium. The application method for the potash fertilizer is beneficial for reasonable fertilization.

The invention relates to sugarcane and provides a method for preparing mixed liquor of glucose and fructose from bagasse. The accession number of the bacterial strain Bacillus aryabhattai GZ03 is CCTCC No: M2013521. The method for preparing mixed liquor of glucose and fructose from bagasse comprises the following steps: 1) activating the bacterial strain Bacillus aryabhattai GZ03 with an activation medium plate, inoculating the bacterial strain in a seed medium and carrying out shake cultivation so as to obtain a seed liquid; 2) inoculating the seed liquid obtained in the step 1) into a sugar-producing medium, continuing shake fermentation cultivation so as to obtain fermentation broth, carrying out centrifugation and then collecting a supernatant; and 3) subjecting the supernatant collected in the step 2) to filtering, wherein the obtained filtrate is the mixed liquor of glucose and fructose. The mixed liquor of glucose and fructose can be used in preparation of food additives and health product additives; the food additives and health product additives may be sweeteners which are especially added into beverages.