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Compound peniroquesine A with anti-inflammatory activity as well as preparation method and application of compound peniroquesine A

A compound and active technology, applied in the field of anti-inflammatory active compound peniroquesine A and its preparation, can solve the problems of difficult to fully utilize the anti-inflammatory activity of sesquiterpene compounds, high cost of sesquiterpene compounds, abuse of antibiotics, etc., to achieve It is easy to realize industrialization, the production method cycle is short, and the effect of less by-products

Active Publication Date: 2018-04-24
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the high cost and long cycle of extracting sesquiterpene compounds from marine organisms, the anti-inflammatory activity of sesquiterpene compounds is difficult to be fully utilized. In order to further promote sesquiterpene compounds, a new anti-inflammatory strategy, which is of great significance to solve the current situation of antibiotic abuse

Method used

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  • Compound peniroquesine A with anti-inflammatory activity as well as preparation method and application of compound peniroquesine A
  • Compound peniroquesine A with anti-inflammatory activity as well as preparation method and application of compound peniroquesine A
  • Compound peniroquesine A with anti-inflammatory activity as well as preparation method and application of compound peniroquesine A

Examples

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preparation example Construction

[0042] The present invention also provides a preparation method of the anti-inflammatory compound peniroquesine A according to the above technical scheme, which comprises the following steps:

[0043] (1) Inoculating Penicillium Louvum into the fermentation medium and fermenting to obtain the fermentation product of Penicillium Louvum;

[0044] The preservation number of the said Penicillium Louvum is CGMCC NO.14140;

[0045] (2) Mixing the fermented product of Penicillium Louvum from the step (1) with an alcohol solution and then ultrasonically extracting to obtain a crude extract of peniroquesine A;

[0046] (3) Purify the crude extract of peniroquesine A by chromatography to obtain the anti-inflammatory active compound peniroquesine A.

[0047] In the present invention, the Penicillium Louvum with the preservation number of CGMCC NO. 14140 is inoculated into the fermentation medium and fermented to obtain the fermentation product of Penicillium Louvum. The fermentation temperature i...

Embodiment 1

[0065] Example 1 Preparation of anti-inflammatory compound peniroquesine A

[0066] 1. Strain activation: Inoculate Penicillium Loudiflorum (preservation number CGMCC NO.14140) on the PDA slant medium, culture at 28°C for 3-7 days, and store in a refrigerator at 4°C for later use.

[0067] 2. Preparation of fermentation medium: Take the washed potatoes, divide them into potato pieces with a diameter of 1 cm, and place them in a tissue culture flask, 50g / bottle. After the tissue culture flask is capped, sterilize it at 120°C for 30 minutes at high temperature, and then cool it. Fermentation medium.

[0068] 3. Inoculate the activated Penicillium louboutin in step 1 into the fermentation medium prepared in step 2 at an inoculum of 1%, cover it and incubate it at 28° C. for 30 days to obtain a fermentation product of Penicillium louboutin.

[0069] 4. Take 50g of the fermented product of Peniroquesine obtained in step 3 and mix with 100mL of methanol according to the mass-volume ratio. T...

Embodiment 2

[0071] Example 2 Identification of structure

[0072] Take the anti-inflammatory compound peniroquesine A prepared in Example 1, and identify the compound peniroquesine by 1D / 2D NMR (one-dimensional nuclear magnetic resonance spectroscopy and two-dimensional nuclear magnetic resonance spectroscopy) and HR-ESI-MS (high resolution electrospray ionization mass spectrometry) A and its structure.

[0073] (1) The HR-ESI-MS spectrum of peniroquesine A is attached figure 1 , See the results figure 2 :

[0074] According to the attached figure 1 And figure 2 It can be seen that the molecular formula of the compound is C 25 H 40 O 2 (m / z:395.2923[M+Na] + , Calculated value: 395.2926), the degree of unsaturation is 6.

[0075] (2) The results of 1D / 2DNMR detection are as attached Figure 3~7 As shown,

[0076] By the attachment image 3 , 4 It can be seen that the compound 1 H and 13 C NMR data show that it contains a double bond (δ H : 5.57s, 1H; δ C : 135.5d), suggesting that the compound co...

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Abstract

The invention relates to the technical field of terpenes and provides a compound peniroquesine A with anti-inflammatory activity. The structure of the compound is shown as formula I in the description. The compound belongs to sesterterpenes and has remarkable anti-inflammatory activity. The compound peniroquesine A with the anti-inflammatory activity is sesterterpenes with novel 5 / 6 / 5 / 6 / 5 five-ring skeleton structure, and variety of the sesterterpenes is enriched. The invention further provides a preparation method of the compound peniroquesine A with anti-inflammatory activity. The compound peniroquesine A is prepared from Penicillium roqueforti by fermenting. The method is simple, fast, low in cost and suitable for large-scale industrial production.

Description

Technical field [0001] The invention relates to the technical field of terpenoids, in particular to an anti-inflammatory active compound peniroquesine A and its preparation method and application. Background technique [0002] Terpenes-hydrocarbons and their oxygen-containing derivatives whose molecular formula is a multiple of isoprene units. They are a large class of natural products with high diversity, including monoterpenes, sesquiterpenes, diterpenes, Disesquiterpenes, triterpenes, etc. Many terpenoid derivatives have been developed as important drugs for the treatment of cancer, bacterial infections, malaria and various other human diseases, so the synthesis of terpenoids is very important. However, due to the fact that the biogenic synthesis pathway has not been fully elucidated, terpenoids have a non-modular structure, and there is no universal and unified synthesis strategy, the source pathways of terpenoids depend on the extraction of natural products. [0003] Disesqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C35/44C12P15/00A61P29/00C12R1/80
CPCC07C35/44C12P15/00
Inventor 丁中涛蔡乐王家鹏俞静
Owner YUNNAN UNIV
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