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A kind of Penicillium loudi for preparing anti-inflammatory active compound peniroquesine A and its application

A technology of Penicillium laurelii and compound, applied in the field of microorganisms, can solve the problems of long cycle, difficult to fully utilize the anti-inflammatory activity of disesquiterpenoids, and high cost of disesquiterpenoids, and achieves short cycle, easy to large The effect of mass production and low cost

Active Publication Date: 2020-04-07
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the high cost and long cycle of extracting sesquiterpene compounds from marine organisms, it is difficult to fully utilize the anti-inflammatory activity of sesquiterpene compounds. pathway for the production of hemiterpenoids

Method used

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  • A kind of Penicillium loudi for preparing anti-inflammatory active compound peniroquesine A and its application
  • A kind of Penicillium loudi for preparing anti-inflammatory active compound peniroquesine A and its application
  • A kind of Penicillium loudi for preparing anti-inflammatory active compound peniroquesine A and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1 isolates Penicillium loudi

[0068] Wash the roots of Aconiti velocifera and soak them in 50% sodium hypochlorite solution for 1 min, then rinse them with sterilized UP water for three times, then soak them in 75% ethanol solution for 1 min, take them out and use the same disinfectant Rinse three times with UP water after sterilization, and dry the water on the surface of aconite with filter paper.

[0069] Peel the sterilized Aconiti root, cut the broken roots no longer than 5mm in length, inoculate 5 pieces of broken roots on the PDA plate, seal the edge of the culture dish with the sealing film, and place it in the GSP-9160MBE compartment. Incubate at a constant temperature of 28°C in a water-type constant temperature incubator.

[0070] Observe once every 24 hours during the cultivation process, pick out the colonies with pure color and form and place them on the surface of a new PDA plate, streak and purify and repeat the above steps until colonies wi...

Embodiment 2

[0072] The colony morphology observation of embodiment 2 Loudi Penicillium

[0073] Penicillium loudii with the preservation number CGMCC NO.14140 was inoculated in PDA medium, cultured at a constant temperature of 27°C, and the changes in colony morphology were observed at 1d, 3d, 5d, and 7d, respectively.

[0074]Penicillium loudii was cultured on PDA plate medium at 27°C. After 1 day, white dot-like fluffy mycelia could be observed on the front of the inoculation point; after 3 days of cultivation, a round white fluffy mycelium with a diameter of about 2 cm was formed around the inoculation point The surface of the colony is relatively flat, the opposite side is light yellow near the center, and the edge is colorless; after 5 days of cultivation, a circular colony with a diameter of about 4 cm is formed around the inoculation point. There are protrusions on the surface of the colony, and the center of the colony contains green particles. Light yellow velvet, the back is yel...

Embodiment 3

[0075] Embodiment 3 prepares anti-inflammatory compound peniroquesine A

[0076] 1. Strain activation: Inoculate Penicillium loudii (preservation number CGMCC NO.14140) on PDA slant medium, culture at 28°C for 3-7 days, and store in a 4°C refrigerator for later use.

[0077] 2. Preparation of fermentation medium: take the washed potatoes, divide them into potato pieces with a diameter of 1cm, put them in a tissue culture bottle, 50g / bottle, cover the tissue culture bottle, sterilize at 120°C for 30min, and cool to obtain Fermentation medium.

[0078] 3. Inoculate the Penicillium loudii activated in step 1 into the fermentation medium prepared in step 2 according to the inoculum amount of 1%, cover and culture at 28° C. for 30 days at a constant temperature to obtain a Penicillium loudii fermentation product.

[0079] 4. Take 50g of the fermented product of Penicillium loudii obtained in step 3 and mix it with 100mL of methanol according to the mass-volume ratio. The mixed sol...

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Abstract

The invention relates to the technical field of microbes, and provides Penicillium roqueforti with a preservation number of CGMCC No.14140. The penicillium loudi provided by the invention is derived from the root of Aconiti japonica, and is an endophytic fungus of Aconiti japonica. The metabolite of Penicillium loudii in the present invention contains a sesquiterpene compound peniroquesine A with a novel 5 / 6 / 5 / 6 / 5 pentacyclic new skeleton structure, and has significant anti-inflammatory activity. The Penicillium loudii provided by the invention can simply and efficiently prepare the sesquiterpene compound peniroquesine A, which is used for preparing anti-inflammatory drugs. The preparation of sesquiterpenoids by microbial fermentation has the advantages of short cycle, mild culture conditions, few by-products, strong stereoselectivity and low cost.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Penicillium roquesine used for preparing an anti-inflammatory active compound peniroquesine A and an application thereof. Background technique [0002] Plant endophytes (endophyte) are fungi or bacteria that live in healthy plant tissues and organs in a certain stage or all stages. Raw bacteria. Plant endophytes are microorganisms that can inhabit healthy plant tissues without causing substantial harm to plants, and have established a harmonious symbiotic relationship with plants. Some plant endophytes can also benefit plants. [0003] Terpenoids—hydrocarbons and their oxygen-containing derivatives with molecular formulas that are multiples of isoprene units, are a large class of natural products with a high degree of diversity, including monoterpenes, sesquiterpenes, diterpenes, Sesquiterpenes, triterpenes, etc. Many terpenoid derivatives have been developed as important d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P15/00A61K31/047A61P29/00C12R1/80
CPCA61K31/047C12P15/00C12N1/145C12R2001/80
Inventor 丁中涛蔡乐王家鹏俞静
Owner YUNNAN UNIV
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