A kind of method of inducing sporulation of filamentous fungus

Abstract

The invention discloses a method for inducing sporulation of hyphosporium fungus, which comprises the following steps: (1) take circular filter paper, evenly cut out more than 4 filter paper holes, lay it flat on a petri dish, add appropriate amount of distilled water to soak, Remove the filter paper after autoclaving. (2) Prepare and sterilize the agar medium, and then add it to the sterilized petri dish, the amount of each petri dish is 15-20ml, and make the agar culture plate after the agar medium is solidified. (3) Spread the sterilized filter paper on the surface of the agar culture plate. (4) Pick an appropriate amount of non-sporogenous hyphomycete fungi and inoculate them on the improved agar culture plate at the center of each filter paper hole. (5) Cover and seal the petri dish, and incubate at room temperature under natural light for 10 to 30 days. The method of the invention has simple operation, mild culture conditions, remarkable effect of inducing sporulation of hyphosporium fungi, is effective for most hyphosporium fungi, conidia are evenly distributed on filter paper, and is convenient for observing sporulation phenotypes.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for inducing sporulation of hyphosporium fungi. Background technique [0002] For a long time, the classification and identification of hyphomycetes have been mainly based on the morphological characteristics of the fungi, and the characteristics of conidia (shape, size, number of septa, surface decoration and appendages, etc.) are the main species-level classification criteria for hyphomycetes. The continuous appearance of conidia (sporulation phenotype) is also an important basis for the identification of hyphosporium species. [0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi smoothly. At the same time, the observ...

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Problems solved by technology

[0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi

At the same time, the observation of sporulation phenotype cannot be carried out at the same time as the production of conidia, and multiple re-cultivation is often required to observe the sporulation phenotype, which consumes a lot of time and experimental costs

[0004] At present, the main methods for promoting hyphosporium sporulation include colony scratching method, ultraviolet light irradiation method, freezing stimulation method, dark / light alternate culture method, etc., but these methods all have the following disadvantages: (1) the ability to promote sporulation The effect is not obvious, the amount of sporulation is small, and the sporulation phenotype is difficult to observe; (2) it is difficult to accurately grasp the treatment time, and improper treatment time will inhibit the sporulation or death of the hyphosporium fungus; (3) the culture cycle is long, often after a long period of time (4) The characteristics of conidia produced are unstable, and there are large differences in the characteristics of conidia produced under different culture conditions

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