28 results about "Agar plate" patented technology

The application discloses a method for improving the sensitivity of a porcine circovirus type 2 Cap protein antigen agar diffusion detection, and belongs to the technical field of veterinary products. The method is as follows: (1) inoculating piglets with a porcine circovirus 2a, 2b and 2d trivalent virus-like particle vaccine, and then separating serum to obtain a porcine circovirus 2a, 2b and 2d trivalent high-immune serum; (2) preparing a high-salt agar plate; (3) diluting the trivalent high-immune serum and the porcine circovirus type 2 Cap protein antigen with a high-salt diluent, respectively, and then adding the diluted trivalent high-immune serum and the porcine circovirus type 2 Cap protein antigen into holes of the agar plate, and incubating at 35-37 DEG C for 20-24 h to complete the detection. The application uses high-immune serum and an improved agar diffusion method to quantitatively detect the content of PCV2 Cap protein, and avoids the defects of low tolerance to buffer reagents and poor detection specificity of the commonly used BCA or Bradford protein content detection method.

This invention discloses an absolute quantification method for pathogens and drug resistance genes in atmospheric bioaerosols and its application. Addressing the bottleneck of achieving absolute quantification of low-concentration samples while maintaining cell integrity and high-concentration nucleic acid extraction, this invention constructs a collection carrier by immobilizing a hydrophilic mixed cellulose ester microporous membrane on a technical agar plate. This is combined with a flow rate ≤30 L / min impactor sampler, ensuring structural integrity while capturing microorganisms, laying the foundation for flow cytometry quantification. This front-end collection system is deeply coupled with a dedicated cascade lysis and concentration process, enabling the acquisition of high-quality DNA suitable for metagenomic library construction and relative abundance analysis even under short-term, low-flow-rate collection. Simultaneously, the total microbial count is absolutely quantified using flow cytometry on the collected samples, and this result is fused with the relative abundance results. Through synergistic optimization of the entire chain, quantitative analysis of species-level pathogens and drug resistance genes in atmospheric bioaerosols is ultimately achieved.

The invention discloses a microplastic degrading enzyme as well as a preparation method and application of the microplastic degrading enzyme. The preparation method comprises the following steps: cloning a fast-petase gene into a pFastBacTM Dual vector, so as to obtain a pFastDual-fast-petase vector; the method comprises the following steps: converting a pFastDual-fast-petase vector into competent cells, coating the competent cells on a flat plate for blue-white shift culture, selecting a white bacterial colony, and extracting recombinant bacmid-fast-petase DNA (Deoxyribose Nucleic Acid); the method comprises the following steps: transfecting recombinant bacmid-fast-petase DNA (deoxyribonucleic acid) into silkworm culture cells, culturing until the cells have diseases, and collecting cell culture supernatant to obtain a recombinant virus rBmNPV-fast-petase; bombyx mori culture cells or bombyx mori are infected with the recombinant virus rBmNPV-fast-petase, after the bombyx mori is attacked, supernate of the bombyx mori culture cells or bombyx mori tissue is collected and then purified, and the microplastic degrading enzyme is obtained. According to the invention, high-yield expression and high-purity preparation of the PET enzyme are realized quickly, and an efficient and sustainable solution is provided for biodegradation of micro-plastic pollution. The success of the invention not only helps to alleviate the global microplastic pollution problem, but also provides important reference for the biodegradation technology of other plastic wastes.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The application discloses a combined drug sensitivity test method for rapidly detecting the synergistic effect of amikacin and ceftazidime-avibactam, and the method comprises the following steps: uniformly coating a to-be-detected bacterial suspension on an MH agar plate, firstly pasting two ATM paper sheets and two CZA paper sheets respectively, and ensuring that the four paper sheets are uniformly distributed on the plate; secondly, pasting a to-be-detected ATM drug sensitivity paper sheet on a to-be-detected CZA paper sheet, and indicating the CZA ATM ; thirdly, pasting a to-be-detected CZA drug sensitivity paper sheet on a to-be-detected ATM paper sheet, and indicating the ATM CZA ; fourthly, respectively dropping 20 μL of sterile normal saline on the CZA ATM and ATM CZA ; and finally, placing the plate in a 35℃ incubator for 8 hours, measuring the diameters of the bacteriostatic rings of ATM, CZA, ATM CZA and CZA ATM , and determining the synergistic effect according to the diameters of the bacteriostatic rings and the drug sensitivity breakpoint standard. The application has the advantages of simple operation, rapidness, accuracy, low cost and the like.

System for the development of a pH-based biosensor using anthocyanins from red cabbage (Brassica oleracea) and chitosan nanoparticles for monitoring food quality, comprising: ◯ Glassware for collecting and processing red cabbage samples, using a system for extracting anthocyanins by: • Thoroughly wash red cabbage with tap water and distilled water; • Chop the washed cabbage into fine pieces of about 1-2 cm; • Soaking 2 kg of shredded cabbage in 100 ml of acidified ethanol solution (ethanol:water:acetic acid, 70:29:1 v / v / v) for 24 hours; • Filter the extract using a vacuum filter and concentrate it under reduced pressure; • Store the extract at 4°C in a dark bottle; ◯ a device for the synthesis of chitosan nanoparticles via ionic gelation, comprising: • Prepare a 0.1% (w / v) chitosan solution in 1% acetic acid, adjusted to pH 4.6; • Add 0.1% tripolyphosphate (TPP) solution dropwise while stirring; • Collecting the nanoparticles by centrifugation and washing with deionized water; ◯ a system for introducing anthocyanins into chitosan nanoparticles by mixing the anthocyanin extract with the chitosan solution prior to TPP addition; ◯ a solution casting system for the production of a pH-sensitive biofilm by mixing anthocyanin-loaded nanoparticles with poly(vinyl alcohol); ◯ analytical instruments, including an Alpha E ATR-FTIR (Bruker, Germany) and an FEI QUANTA 450 scanning electron microscope, for characterizing the nanoparticles and biofilm; ◯ Design of a well diffusion test to assess antibacterial activity against Staphylococcus aureus using Mueller-Hinton agar plates.

The invention relates to the technical field of biological monitoring, in particular to a preparation method and application of a blood plate. The preparation method of the blood plate comprises the following steps: adding culture medium components into a culture medium, the culture medium components comprising peptone nitrogen, a yeast extract, reduced glutathione, sodium pyruvate and distilled water; adding blood into the culture medium; adjusting the pH value of the culture medium to be stable; adding a heat-sensitive component and a heat-stable component into the culture medium; and constructing a culture medium low-oxygen mixing environment. Therefore, the shelf life of the blood plate can be prolonged, and the hemolysis phenomenon is reduced.

This invention discloses a method for improving the high yield of extracellular polysaccharides from Bacillus belye on agar plates based on agricultural waste, belonging to the fields of microbial fermentation technology and resource utilization of agricultural waste. The method involves inoculating Bacillus belye seed culture into agar plates containing agricultural waste, culturing at 36-38°C to obtain fermentation products, and extracting the extracellular polysaccharides from the fermentation products. The agar plate containing agricultural waste comprises: peptone 9-11 g / L, yeast extract 4.5-5.5 g / L, NaCl 9.5-10.5 g / L, agar 17-19 g / L, agricultural waste powder 10-15 g / L, and pH 7.1-7.3. This method solves the problems of low yield, high cost, and high energy consumption associated with traditional liquid shake-flask culture, while simultaneously achieving high-value utilization of agricultural waste.

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The present disclosure provides systems and methods for detecting the presence of colonies on agar plates. The systems and methods are preferably useful for assessing the presence of contaminants (e.g., bacteria, fungi, etc.) in samples, such as raw materials or components of pharmaceutical compositions, by analyzing whether the sample forms colonies on an agar plate. The systems use lighting arrangements to enhance the accuracy of the analysis and, in embodiments, include the use of artificial intelligence as part of the automated process.

The invention discloses a giant strong aeromonas hydrophila bacteriophage and application thereof, and belongs to the technical field of microorganisms. According to the invention, aeromonas hydrophila is taken as host bacteria, the phage is enriched from a collected lake water sample, and is separated and purified by adopting a double-layer agar plate method, so that a strain of phage is successfully obtained, the strain of phage is named as aeromonas hydrophila phage Cgtyf, the preservation number is CCTCC NO: M 2026099, and the titer of the phage can reach 1.0 * 10 < 10 > PFU / mL; the genome size of the phage is about 237 kb, and the phage belongs to a giant phage. In an in-vitro bacteriostasis experiment, the bacteriophage Cgtyf shows a remarkable bactericidal effect and a relatively strong growth inhibition capability on host bacteria, and shows a relatively high temperature tolerance range in a temperature stability test. Therefore, the bacteriophage Cgtyf provided by the invention can be effectively used for preventing and controlling aeromonas hydrophila in aquatic products, has a potential application value as an antibacterial drug substitute, is beneficial to reducing or avoiding the use of antibacterial drugs in aquaculture, and has a good application prospect.

The invention discloses a purification method of buckwheat white mold pathogenic bacteria, which belongs to the technical field of biology, and comprises the following steps: S1, inoculating buckwheat white mold pathogenic bacteria spores on a PDA solid plate culture medium by using a sterilized brush or a single cell picking method, and culturing for 10 days; s2, punching the culture medium obtained in the step S1 by using a fungus cake puncher to obtain a fungus cake; s3, the fungus cakes obtained in the step S2 do circular motion on the edge of a new PDA culture medium to rub back and forth, so that white mold spores on the fungus cakes are rubbed to the culture medium, and culture is conducted. When the purification method disclosed by the invention is used for culturing the buckwheat white mold pathogenic bacteria, a large number of target strains after purification culture can be obtained about 10 days after a new PDA culture medium is inoculated with a bacterial cake.

The invention discloses a fish intestinal tract L-dopa decarboxylation strain as well as a separation, purification and identification method and application thereof, and belongs to the technical field of microorganisms. The bacterial strain is identified as enterococcus faecium SAGEF001, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20252372. The target strain is successfully screened from the scatophagus argus intestinal tract through BHI enrichment and PSE agar plate directional separation under the aerobic condition, and is identified as the fish intestinal tract L-dopa decarboxylated strain through morphology, molecular biology (16S rRNA gene V3-V4 region sequencing) and functionality (dopamine content detection), and a fish microorganism resource library can be enriched. The strain can efficiently decarboxylate L-dopa to generate dopamine, and can be used for preparing a microbial agent for aquaculture health regulation or environmental restoration.

A bacterial colony-forming-unit (CFU) detection system is disclosed that exploits a thin-film-transistor (TFT)-based image sensor array that saves ˜12 hours compared to the Environmental Protection Agency (EPA)-approved methods. A lensfree imaging modality was built using the TFT image sensor with a sample field-of-view of ˜10 cm2. Time-lapse images of bacterial colonies cultured on chromogenic agar plates were automatically collected at 5-minute intervals. Two deep neural networks were used to detect and count the growing colonies and identify their species. When blindly tested with 265 colonies of E. coli and other coliform bacteria (i.e., Citrobacter and Klebsiella pneumoniae), the system reached an average CFU detection rate of 97.3% at 9 hours of incubation and an average recovery rate of 91.6% at ˜12 hours. This TFT-based sensor can be applied to various microbiological detection methods. The imaging field-of-view of this platform can be cost-effectively increased to >100 cm2.

The utility model discloses a traditional Chinese medicine sensitive agar plate puncher which comprises a base column, the base column is provided with a cylindrical hollow part, internal threads are arranged at the cylindrical hollow part, and a first screw is matched with the hollow part, so that the free end of the first screw can rotationally penetrate through the cylindrical hollow part to extend to the outside and is fixed through a nut; the plurality of brackets are uniformly distributed on the periphery of the foundation pillar; each bracket is provided with a rectangular hollow part, and the inner circumference of each rectangular hollow part is coated with a rubber gasket; the second screw is arranged at the rectangular hollow part, and the second screw is matched with the rectangular hollow part, so that the free end of the second screw can penetrate through the rectangular hollow part to extend to the outside and is fixed through a nut; and the second screw can translate or slide up and down in the rectangular hollow part. According to the hole puncher, the steps of bottom sealing and agar picking after punching are omitted, and meanwhile punched holes are uniform and attractive in distribution.

The utility model discloses a kind of excrement fungus microbial separation devices, including back type frame, the inside of back type frame is fixed with tray by supporting member, and the top end of the tray is arrayed with several circular lower grooves, and circular lower groove is placed with agar plate;C character sliding platform, the top end of back type frame is slidably installed in C character sliding platform, the top end of C character sliding platform is fixed with C character frame, and the both sides of C character frame inside are slidably installed with guide rod, the inside of C character frame is installed with the elastic down-pressing structure for forcing guide rod to go down.The utility model can help staff to quickly complete the regional division operation of strain, compared with each subarea, the device can handle multiple plates simultaneously, save a lot of time and labor cost of staff, and using the device can ensure that the partition position and size of each agar plate are consistent, can avoid error and inconsistency in manual operation.

The utility model relates to a big flow biological sol of adjustable flow sampling device, including air filter body and sampling mechanism, the upper end of air filter body is equipped with the air inlet, air filter body lower extreme is equipped with the air outlet, the air outlet is linked with the air pump, still be equipped with screen between the air inlet and air outlet, sampling mechanism sets up in the below of screen, sampling mechanism includes sampling cotton. The utility model can effectively solve the problem that the existing agar plate sampling device is in the long -time sampling process because of the airflow impact and leads to the culture medium water loss, cannot gather virus and the problem that the detection range is limited, and sampling efficiency is high, and sampling effect is good.

The application discloses a single-cell blue algae separation and purification method based on rotifer enrichment and application, and the method comprises the following steps: placing a single-cell blue algae solution into a sterile BG11 conical flask; adding a rotifer solution with a known concentration into the sterile BG11 conical flask containing the single-cell blue algae solution to obtain a mixed solution of the rotifer and the single-cell blue algae; placing the sterile BG11 conical flask containing the mixed solution of the rotifer and the single-cell blue algae in a first culture environment for culture to obtain a blue algae and rotifer enrichment solution; transferring the blue algae and rotifer enrichment solution to a sterilized BG11 agar plate for plate coating treatment, and inverting the BG11 agar plate in a second culture environment for culture until a blue algae colony without bacteria is formed; and expanding the blue algae colony without bacteria to obtain single-cell blue algae without bacteria. The application can greatly shorten the time for traditional separation and culture of single-cell blue algae, and can prevent bacterial pollution from an external environment.

This utility model discloses a plate punching device for antibacterial experiments, including a puncher body with a punching head for punching agar plates at one end; a push rod coaxially arranged with the punching head; and a push rod pressing part located at the other end of the puncher body. By pressing the push rod pressing part, the push rod moves along its own axis to push out the agar block stuck in the punching head. This utility model, by setting a push rod and a push rod pressing part coaxial with the punching head, allows the experimenter to simply press the push rod pressing part after punching to move the push rod along its own axis and push out the agar block stuck in the punching head. This allows the experimenter to complete the punching process more smoothly, reduces operation interruption time, improves the convenience and efficiency of agar block removal, and reduces the complexity of the experimental operation.

The invention discloses a bio-based oil displacement agent for improving recovery efficiency and a preparation method of the bio-based oil displacement agent. The preparation method comprises the following steps that bacillus subtilis BZ-1 is subjected to activation culture on an LB culture medium plate, the bacillus subtilis BZ-1 is preserved in the China General Microbiological Culture Collection Center on November 15, 2024, and the preservation number is CGMCC No.32653; the preparation method comprises the following steps: inoculating a single colony on a flat plate into an LB liquid culture medium for culturing, carrying out enlarged culture, then transferring to a fermentation culture medium, carrying out preliminary filtration, wall breaking treatment and fine filtration on the cultured bacterial liquid, and then compounding with decyl hydroxypropyl sulfobetaine and fatty alcohol-polyoxyethylene ether ammonium sulfate to obtain the bio-based oil displacement agent. The bio-based oil-displacing agent can effectively reduce surface tension and oil-water interfacial tension, has a remarkable oil-displacing effect, and can solve the problem of low oil recovery rate of low-permeability oil reservoirs.

The invention relates to the technical field of agricultural biology, in particular to a lyase compound preparation and application thereof in prevention and treatment of citrus canker, and the lyase compound preparation comprises lyase preparations LysXAC2, LysXAC4 and LysXAC5. An in-vitro bacteriostasis experiment shows that the lyase preparation forms an obvious splitting ring on an agar plate for the xanthomonas carpesium, and the growth of the xanthomonas carpesium can be effectively inhibited within 72 hours. Field application tests show that 25 L of the preparation is sprayed per mu after the preparation is diluted by 1000 times, the preparation starts to be applied in the early disease stage or the prevention stage and is applied once every 5 days, and the control effect can reach 83% or above after the preparation is continuously applied for 5 times. The preparation disclosed by the invention has the advantages of efficient sterilization, safety in use, environmental friendliness and the like, and a novel effective solution is provided for green prevention and control of citrus canker.

The present application belongs to the technical field of edible fungi, and particularly relates to a culture method of pleurotus ostreatus mycelium rich in essential amino acids and polysaccharides. In the process of expanding the culture of pleurotus ostreatus, different time is treated under a magnetic field environment; the different magnetic field treatment time is 24-180 h, the magnetic field strength is 10 mT, and the magnetic field alternating frequency is 50 Hz; 0.6-1.2 mmol / L different proportions of Mg 2+ , Zn 2+ and Mn 2+ trace elements are added to the special PDA agar plate respectively. The beneficial effects of the present application are that the weight of the pleurotus ostreatus mycelium, the proportion of essential amino acids and the polysaccharide content are respectively increased by 56.16%, 44.09% and 27.52% compared with the control group after the culture medium is added with trace elements of a certain concentration and composition and treated by a magnetic field. The present application provides technical reference for the production and application expansion of pleurotus ostreatus and the improvement of nutritional value.

The invention belongs to the technical field of microbial fermentation, discloses a liquid fermentation optimization process of a biocontrol compound bacterium system for effectively inhibiting ganoderma lucidum mycorrhizal rot, and aims to optimize a biocontrol compound bacterium system fermentation culture medium and fermentation conditions constructed by pseudomonas aeruginosa and bacillus licheniformis by taking the size of a plate antagonistic ganoderma lucidum bacterium inhibition zone as a target. The optimized culture medium comprises the following main components: 3% of cane sugar, 0.5% of ammonium sulfate, 1% of beef extract and 2% of potassium chloride; the optimal fermentation conditions are as follows: the temperature is 32 DEG C, the initial pH value is 7, the inoculum size is 3%, the time is 24 hours, and the rotating speed is 220 rpm. The inhibition rate of the optimized compound bacterium system on ganoderma lucidum is increased to 88.7%. According to the method for optimizing the fermentation culture medium and the fermentation conditions of the compound bacterium system, the biocontrol capability can be improved, the culture time can be shortened, the cost can be saved, and a theoretical basis is provided for subsequent development and application of a biocontrol bacterium agent for preventing and treating the delonix regia root rot.

The utility model discloses a culture medium bottle for preparing a Columbia blood agar plate, and relates to the technical field of culture medium bottles. The culture medium bottle for preparing the Columbia blood agar plate comprises a culture medium bottle body, and the conveying mechanism comprises a fixing pipe, a mounting sleeve, a suction component and a conveying component, and the outer surface of the fixing pipe is fixedly sleeved with the mounting sleeve. According to the culture medium bottle for preparing the Columbia blood agar plate, a fixing motor drives a fixing threaded rod to rotate, a threaded cylinder pushes a fixing block and a rubber plug to move, and culture medium liquid in the culture medium bottle body enters a fixing pipe through a suction pipe and a first one-way valve and then passes through the fixing motor; the fixing block and the rubber plug push the culture medium liquid, the culture medium liquid is conveyed out of the fixing pipe through the second one-way valve and the conveying pipe, the culture medium liquid can be conveyed into the culture dishes, the amount of the culture medium liquid in each culture dish can be the same, and therefore the accuracy and consistency of experimental results are kept.

The utility model relates to the technical field of medical instruments, in particular to an immunoglobulin electrophoresis inspection device which comprises an electrophoresis inspection device body, an agar plate push plate is mounted on one side of the electrophoresis inspection device body, and a handle is fixedly connected to one side of the agar plate push plate. And an electrophoresis inspection groove is formed in one side of the electrophoresis inspection device body. The electrophoresis device has the advantages that the four leveling screw rods are respectively rotated to control the lengths of the leveling screw rods protruding out of the first convex blocks, so that the heights of the four corners of the electrophoresis device body are adjusted, and whether the whole device is horizontal or not is judged by matching with the two vertically arranged bubble levels; and the leveling screw rod can be rotated more conveniently through the rotary knob, and the supporting legs can have a larger rotation angle and range through the cooperation of the spherical connecting head and the spherical connecting groove, so that the supporting legs can be better attached to the placing surface to support the whole device.

The invention discloses a quantitative evaluation method for drug resistance of a microbial community, which comprises the following steps of: accurately positioning the position of a single colony on an agar plate by combining a paper diffusion method and colony image recognition processing based on the microbial community extracted from an environmental sample, and further carrying out quantitative analysis on the sample according to the colony distribution condition. And finally, calculating to obtain the drug resistance level of the microbial community through grading and weight matching of the drug-resistant areas. The method is provided for accurately and quantitatively evaluating the community drug resistance level of environmental microbial samples for environmental samples such as soil, natural water and activated sludge, and has high application value in the aspects of risk evaluation and prevention and control of drug resistance of environmental microbial communities.

This invention provides a method for isolating target microorganisms, which are rare biospheres, from environmental microbial samples. [Solution] A method for isolating target microorganisms, which are rare biospheres, from an environmental microbial sample, comprising the steps of: preparing donor DNA, which is nucleic acid around the sequence of an antibiotic susceptibility gene, to perform a single nucleotide substitution on the antibiotic susceptibility gene of the target microorganism; introducing the donor DNA into the environmental microbial sample and performing a single nucleotide substitution on the antibiotic binding site of the target microorganism; accumulating and culturing the environmental microbial sample in a liquid medium containing the antibiotic; and separating the microorganism after accumulation and culturing by the agar plate method or the ultradilution method.

The utility model provides an agar plate preparation device for immunodiffusion test, which comprises a culture dish, a culture dish cover and a quincuncial pile column arranged on the culture dish cover, and further comprises a middle cover, the middle cover is detachably arranged at an opening of the culture dish, the culture dish cover is covered on the middle cover, and the quincuncial pile column is arranged on the culture dish cover. The middle cover is provided with a penetrating opening for the quincuncial pile column to penetrate through, and the middle cover and the culture dish cover are provided with at least one pair of vertical guide structures matched with each other. The opening track of the culture dish cover is limited, so that the inherent shape of an agar plate is prevented from being damaged when the quincuncial pile column is pulled out of the agar plate, and the preparation efficiency and the qualification rate of the agar plate in the immunodiffusion test process are improved.