13 results about "Agar plate" patented technology

This invention discloses an absolute quantification method for pathogens and drug resistance genes in atmospheric bioaerosols and its application. Addressing the bottleneck of achieving absolute quantification of low-concentration samples while maintaining cell integrity and high-concentration nucleic acid extraction, this invention constructs a collection carrier by immobilizing a hydrophilic mixed cellulose ester microporous membrane on a technical agar plate. This is combined with a flow rate ≤30 L / min impactor sampler, ensuring structural integrity while capturing microorganisms, laying the foundation for flow cytometry quantification. This front-end collection system is deeply coupled with a dedicated cascade lysis and concentration process, enabling the acquisition of high-quality DNA suitable for metagenomic library construction and relative abundance analysis even under short-term, low-flow-rate collection. Simultaneously, the total microbial count is absolutely quantified using flow cytometry on the collected samples, and this result is fused with the relative abundance results. Through synergistic optimization of the entire chain, quantitative analysis of species-level pathogens and drug resistance genes in atmospheric bioaerosols is ultimately achieved.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The application discloses a combined drug sensitivity test method for rapidly detecting the synergistic effect of amikacin and ceftazidime-avibactam, and the method comprises the following steps: uniformly coating a to-be-detected bacterial suspension on an MH agar plate, firstly pasting two ATM paper sheets and two CZA paper sheets respectively, and ensuring that the four paper sheets are uniformly distributed on the plate; secondly, pasting a to-be-detected ATM drug sensitivity paper sheet on a to-be-detected CZA paper sheet, and indicating the CZA ATM ; thirdly, pasting a to-be-detected CZA drug sensitivity paper sheet on a to-be-detected ATM paper sheet, and indicating the ATM CZA ; fourthly, respectively dropping 20 μL of sterile normal saline on the CZA ATM and ATM CZA ; and finally, placing the plate in a 35℃ incubator for 8 hours, measuring the diameters of the bacteriostatic rings of ATM, CZA, ATM CZA and CZA ATM , and determining the synergistic effect according to the diameters of the bacteriostatic rings and the drug sensitivity breakpoint standard. The application has the advantages of simple operation, rapidness, accuracy, low cost and the like.

This invention discloses a method for improving the high yield of extracellular polysaccharides from Bacillus belye on agar plates based on agricultural waste, belonging to the fields of microbial fermentation technology and resource utilization of agricultural waste. The method involves inoculating Bacillus belye seed culture into agar plates containing agricultural waste, culturing at 36-38°C to obtain fermentation products, and extracting the extracellular polysaccharides from the fermentation products. The agar plate containing agricultural waste comprises: peptone 9-11 g / L, yeast extract 4.5-5.5 g / L, NaCl 9.5-10.5 g / L, agar 17-19 g / L, agricultural waste powder 10-15 g / L, and pH 7.1-7.3. This method solves the problems of low yield, high cost, and high energy consumption associated with traditional liquid shake-flask culture, while simultaneously achieving high-value utilization of agricultural waste.

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The invention discloses a giant strong aeromonas hydrophila bacteriophage and application thereof, and belongs to the technical field of microorganisms. According to the invention, aeromonas hydrophila is taken as host bacteria, the phage is enriched from a collected lake water sample, and is separated and purified by adopting a double-layer agar plate method, so that a strain of phage is successfully obtained, the strain of phage is named as aeromonas hydrophila phage Cgtyf, the preservation number is CCTCC NO: M 2026099, and the titer of the phage can reach 1.0 * 10 < 10 > PFU / mL; the genome size of the phage is about 237 kb, and the phage belongs to a giant phage. In an in-vitro bacteriostasis experiment, the bacteriophage Cgtyf shows a remarkable bactericidal effect and a relatively strong growth inhibition capability on host bacteria, and shows a relatively high temperature tolerance range in a temperature stability test. Therefore, the bacteriophage Cgtyf provided by the invention can be effectively used for preventing and controlling aeromonas hydrophila in aquatic products, has a potential application value as an antibacterial drug substitute, is beneficial to reducing or avoiding the use of antibacterial drugs in aquaculture, and has a good application prospect.

The utility model discloses a kind of excrement fungus microbial separation devices, including back type frame, the inside of back type frame is fixed with tray by supporting member, and the top end of the tray is arrayed with several circular lower grooves, and circular lower groove is placed with agar plate;C character sliding platform, the top end of back type frame is slidably installed in C character sliding platform, the top end of C character sliding platform is fixed with C character frame, and the both sides of C character frame inside are slidably installed with guide rod, the inside of C character frame is installed with the elastic down-pressing structure for forcing guide rod to go down.The utility model can help staff to quickly complete the regional division operation of strain, compared with each subarea, the device can handle multiple plates simultaneously, save a lot of time and labor cost of staff, and using the device can ensure that the partition position and size of each agar plate are consistent, can avoid error and inconsistency in manual operation.

The utility model relates to a big flow biological sol of adjustable flow sampling device, including air filter body and sampling mechanism, the upper end of air filter body is equipped with the air inlet, air filter body lower extreme is equipped with the air outlet, the air outlet is linked with the air pump, still be equipped with screen between the air inlet and air outlet, sampling mechanism sets up in the below of screen, sampling mechanism includes sampling cotton. The utility model can effectively solve the problem that the existing agar plate sampling device is in the long -time sampling process because of the airflow impact and leads to the culture medium water loss, cannot gather virus and the problem that the detection range is limited, and sampling efficiency is high, and sampling effect is good.

This utility model discloses a plate punching device for antibacterial experiments, including a puncher body with a punching head for punching agar plates at one end; a push rod coaxially arranged with the punching head; and a push rod pressing part located at the other end of the puncher body. By pressing the push rod pressing part, the push rod moves along its own axis to push out the agar block stuck in the punching head. This utility model, by setting a push rod and a push rod pressing part coaxial with the punching head, allows the experimenter to simply press the push rod pressing part after punching to move the push rod along its own axis and push out the agar block stuck in the punching head. This allows the experimenter to complete the punching process more smoothly, reduces operation interruption time, improves the convenience and efficiency of agar block removal, and reduces the complexity of the experimental operation.

The invention discloses a bio-based oil displacement agent for improving recovery efficiency and a preparation method of the bio-based oil displacement agent. The preparation method comprises the following steps that bacillus subtilis BZ-1 is subjected to activation culture on an LB culture medium plate, the bacillus subtilis BZ-1 is preserved in the China General Microbiological Culture Collection Center on November 15, 2024, and the preservation number is CGMCC No.32653; the preparation method comprises the following steps: inoculating a single colony on a flat plate into an LB liquid culture medium for culturing, carrying out enlarged culture, then transferring to a fermentation culture medium, carrying out preliminary filtration, wall breaking treatment and fine filtration on the cultured bacterial liquid, and then compounding with decyl hydroxypropyl sulfobetaine and fatty alcohol-polyoxyethylene ether ammonium sulfate to obtain the bio-based oil displacement agent. The bio-based oil-displacing agent can effectively reduce surface tension and oil-water interfacial tension, has a remarkable oil-displacing effect, and can solve the problem of low oil recovery rate of low-permeability oil reservoirs.

The present application belongs to the technical field of edible fungi, and particularly relates to a culture method of pleurotus ostreatus mycelium rich in essential amino acids and polysaccharides. In the process of expanding the culture of pleurotus ostreatus, different time is treated under a magnetic field environment; the different magnetic field treatment time is 24-180 h, the magnetic field strength is 10 mT, and the magnetic field alternating frequency is 50 Hz; 0.6-1.2 mmol / L different proportions of Mg 2+ , Zn 2+ and Mn 2+ trace elements are added to the special PDA agar plate respectively. The beneficial effects of the present application are that the weight of the pleurotus ostreatus mycelium, the proportion of essential amino acids and the polysaccharide content are respectively increased by 56.16%, 44.09% and 27.52% compared with the control group after the culture medium is added with trace elements of a certain concentration and composition and treated by a magnetic field. The present application provides technical reference for the production and application expansion of pleurotus ostreatus and the improvement of nutritional value.

The invention discloses a quantitative evaluation method for drug resistance of a microbial community, which comprises the following steps of: accurately positioning the position of a single colony on an agar plate by combining a paper diffusion method and colony image recognition processing based on the microbial community extracted from an environmental sample, and further carrying out quantitative analysis on the sample according to the colony distribution condition. And finally, calculating to obtain the drug resistance level of the microbial community through grading and weight matching of the drug-resistant areas. The method is provided for accurately and quantitatively evaluating the community drug resistance level of environmental microbial samples for environmental samples such as soil, natural water and activated sludge, and has high application value in the aspects of risk evaluation and prevention and control of drug resistance of environmental microbial communities.

This invention provides a method for isolating target microorganisms, which are rare biospheres, from environmental microbial samples. [Solution] A method for isolating target microorganisms, which are rare biospheres, from an environmental microbial sample, comprising the steps of: preparing donor DNA, which is nucleic acid around the sequence of an antibiotic susceptibility gene, to perform a single nucleotide substitution on the antibiotic susceptibility gene of the target microorganism; introducing the donor DNA into the environmental microbial sample and performing a single nucleotide substitution on the antibiotic binding site of the target microorganism; accumulating and culturing the environmental microbial sample in a liquid medium containing the antibiotic; and separating the microorganism after accumulation and culturing by the agar plate method or the ultradilution method.