41 results about "Agar plate" patented technology

A bacterial colony forming unit (CFU) detection system is disclosed that utilizes a thin film transistor (TFT)-based image sensor array that saves about 12 hours compared to methods approved by Environmental Protection Agency (EPA). Lensless imaging modes are constructed using TFT image sensors having a sample field of view of about 10 cm2. And automatically collecting delay images of bacterial colonies cultured on the chromogenic agar plate every 5 minutes. Two deep neural networks are used to detect and count colonies in growth and identify their species. When 265 escherichia coli and other coliforms (namely citrobacter and klebsiella pneumoniae) colonies are used for blind detection, the average CFU detection rate of the system reaches 97.3% when the system is cultured for 9 hours, and the average recovery rate of the system reaches 91.6% when the system is cultured for about 12 hours. The TFT-based sensor can be applied to various microbiological detection methods. The imaging field of view of the platform can be economically and efficiently increased to more than 100cm < 2 >.

The invention discloses a paramecium enrichment-based single-cell cyanobacteria separation and purification method and application. The method comprises the following steps: putting a single-cell cyanobacteria solution into a sterile BG11 conical flask; the method comprises the following steps: adding a paramecium solution with known concentration into a sterile BG11 conical flask filled with a single-cell cyanobacteria solution to obtain a mixed solution of paramecium and single-cell cyanobacteria; placing the sterile BG11 conical flask filled with the mixed solution of paramecia and single-cell blue-green algae in a first culture environment for culture to obtain a blue-green algae and paramecia enriched solution; transferring the blue-green algae and paramecium enrichment solution to a sterilized BG11 agar plate for plate coating treatment, and inversely placing the BG11 agar plate in a second culture environment for culture until blue-green algae falling without infectious microbes is formed; and carrying out expanding culture on the bacterial-free cyanobacteria colony to obtain bacterial-free single-cell cyanobacteria. The method can greatly shorten the time of traditional isolated culture of single-cell cyanobacteria, and can prevent bacterial pollution of the external environment.

The present invention discloses a labeling barcode printing system dedicated to the side wall of a blood agar plate, which includes a barcode scanner, a barcode printing unit, a negative pressure adsorption unit, and X-direction, Y-direction, and Z-direction moving units; the barcode scanner is used to scan and read the label barcode information on the side wall of the blood agar plate and send it to the upper computer; the barcode printing unit includes a barcode printer and a label sending position; the X-direction moving unit is used to drive the blood agar plate tray to reciprocate between the barcode scanner and the label pasting position; the Y-direction moving unit is used to drive the third bracket to reciprocate along the Y-direction; the Z-direction moving unit is used to drive the negative pressure adsorption unit to reciprocate along the Z-direction. The present invention realizes the automation of label barcode printing and side wall labeling of blood agar plates, with high accuracy of label barcode pasting, reduced costs, and less operator participation required in the pasting process, avoiding biosafety risks.

The application discloses a method for improving the sensitivity of a porcine circovirus type 2 Cap protein antigen agar diffusion detection, and belongs to the technical field of veterinary products. The method is as follows: (1) inoculating piglets with a porcine circovirus 2a, 2b and 2d trivalent virus-like particle vaccine, and then separating serum to obtain a porcine circovirus 2a, 2b and 2d trivalent high-immune serum; (2) preparing a high-salt agar plate; (3) diluting the trivalent high-immune serum and the porcine circovirus type 2 Cap protein antigen with a high-salt diluent, respectively, and then adding the diluted trivalent high-immune serum and the porcine circovirus type 2 Cap protein antigen into holes of the agar plate, and incubating at 35-37 DEG C for 20-24 h to complete the detection. The application uses high-immune serum and an improved agar diffusion method to quantitatively detect the content of PCV2 Cap protein, and avoids the defects of low tolerance to buffer reagents and poor detection specificity of the commonly used BCA or Bradford protein content detection method.

This invention discloses an absolute quantification method for pathogens and drug resistance genes in atmospheric bioaerosols and its application. Addressing the bottleneck of achieving absolute quantification of low-concentration samples while maintaining cell integrity and high-concentration nucleic acid extraction, this invention constructs a collection carrier by immobilizing a hydrophilic mixed cellulose ester microporous membrane on a technical agar plate. This is combined with a flow rate ≤30 L / min impactor sampler, ensuring structural integrity while capturing microorganisms, laying the foundation for flow cytometry quantification. This front-end collection system is deeply coupled with a dedicated cascade lysis and concentration process, enabling the acquisition of high-quality DNA suitable for metagenomic library construction and relative abundance analysis even under short-term, low-flow-rate collection. Simultaneously, the total microbial count is absolutely quantified using flow cytometry on the collected samples, and this result is fused with the relative abundance results. Through synergistic optimization of the entire chain, quantitative analysis of species-level pathogens and drug resistance genes in atmospheric bioaerosols is ultimately achieved.

The invention discloses a method for improving agar diffusion detection sensitivity of a porcine circovirus type 2 Cap protein antigen, and belongs to the technical field of veterinary products. The method comprises the following steps: (1) inoculating a piglet with a porcine circovirus 2a, 2b and 2d type trivalent virus-like particle vaccine, and separating serum to obtain porcine circovirus 2a, 2b and 2d type trivalent hyperimmune serum; (2) preparing a high-salt agar plate; and (3) respectively diluting the trivalent hyper-immune serum and the porcine circovirus type 2 Cap protein antigen by using a high-salt diluent, then adding the diluted trivalent hyper-immune serum and the porcine circovirus type 2 Cap protein antigen into holes of an agar plate, and incubating for 20-24 hours at 35-37 DEG C to complete detection. According to the method, the PCV2 Cap protein content is quantified by using hyper-immune serum and an improved agar diffusion method, so that the defects that a common BCA or Bradford protein content detection method is low in buffer reagent tolerance, poor in detection specificity and the like are overcome.

The invention discloses a microplastic degrading enzyme as well as a preparation method and application of the microplastic degrading enzyme. The preparation method comprises the following steps: cloning a fast-petase gene into a pFastBacTM Dual vector, so as to obtain a pFastDual-fast-petase vector; the method comprises the following steps: converting a pFastDual-fast-petase vector into competent cells, coating the competent cells on a flat plate for blue-white shift culture, selecting a white bacterial colony, and extracting recombinant bacmid-fast-petase DNA (Deoxyribose Nucleic Acid); the method comprises the following steps: transfecting recombinant bacmid-fast-petase DNA (deoxyribonucleic acid) into silkworm culture cells, culturing until the cells have diseases, and collecting cell culture supernatant to obtain a recombinant virus rBmNPV-fast-petase; bombyx mori culture cells or bombyx mori are infected with the recombinant virus rBmNPV-fast-petase, after the bombyx mori is attacked, supernate of the bombyx mori culture cells or bombyx mori tissue is collected and then purified, and the microplastic degrading enzyme is obtained. According to the invention, high-yield expression and high-purity preparation of the PET enzyme are realized quickly, and an efficient and sustainable solution is provided for biodegradation of micro-plastic pollution. The success of the invention not only helps to alleviate the global microplastic pollution problem, but also provides important reference for the biodegradation technology of other plastic wastes.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The application discloses a combined drug sensitivity test method for rapidly detecting the synergistic effect of amikacin and ceftazidime-avibactam, and the method comprises the following steps: uniformly coating a to-be-detected bacterial suspension on an MH agar plate, firstly pasting two ATM paper sheets and two CZA paper sheets respectively, and ensuring that the four paper sheets are uniformly distributed on the plate; secondly, pasting a to-be-detected ATM drug sensitivity paper sheet on a to-be-detected CZA paper sheet, and indicating the CZA ATM ; thirdly, pasting a to-be-detected CZA drug sensitivity paper sheet on a to-be-detected ATM paper sheet, and indicating the ATM CZA ; fourthly, respectively dropping 20 μL of sterile normal saline on the CZA ATM and ATM CZA ; and finally, placing the plate in a 35℃ incubator for 8 hours, measuring the diameters of the bacteriostatic rings of ATM, CZA, ATM CZA and CZA ATM , and determining the synergistic effect according to the diameters of the bacteriostatic rings and the drug sensitivity breakpoint standard. The application has the advantages of simple operation, rapidness, accuracy, low cost and the like.

System for the development of a pH-based biosensor using anthocyanins from red cabbage (Brassica oleracea) and chitosan nanoparticles for monitoring food quality, comprising: ◯ Glassware for collecting and processing red cabbage samples, using a system for extracting anthocyanins by: • Thoroughly wash red cabbage with tap water and distilled water; • Chop the washed cabbage into fine pieces of about 1-2 cm; • Soaking 2 kg of shredded cabbage in 100 ml of acidified ethanol solution (ethanol:water:acetic acid, 70:29:1 v / v / v) for 24 hours; • Filter the extract using a vacuum filter and concentrate it under reduced pressure; • Store the extract at 4°C in a dark bottle; ◯ a device for the synthesis of chitosan nanoparticles via ionic gelation, comprising: • Prepare a 0.1% (w / v) chitosan solution in 1% acetic acid, adjusted to pH 4.6; • Add 0.1% tripolyphosphate (TPP) solution dropwise while stirring; • Collecting the nanoparticles by centrifugation and washing with deionized water; ◯ a system for introducing anthocyanins into chitosan nanoparticles by mixing the anthocyanin extract with the chitosan solution prior to TPP addition; ◯ a solution casting system for the production of a pH-sensitive biofilm by mixing anthocyanin-loaded nanoparticles with poly(vinyl alcohol); ◯ analytical instruments, including an Alpha E ATR-FTIR (Bruker, Germany) and an FEI QUANTA 450 scanning electron microscope, for characterizing the nanoparticles and biofilm; ◯ Design of a well diffusion test to assess antibacterial activity against Staphylococcus aureus using Mueller-Hinton agar plates.

The utility model discloses a humidity-controllable antrodia camphorata culture dish, which relates to the technical field of antrodia camphorata culture equipment and comprises a culture room, a through hole partition plate is arranged in the culture room, a cover is sleeved at the top end of the culture room, a cover plate is arranged in the middle of the top end of the cover, and vent holes are formed in the outer surface of the cover plate. Guide rails are fixedly installed on the front side and the rear side of the top end of the cover, limiting strips are arranged in the left sides and the right sides of the two guide rails, the two sides of a first movable cover and a second movable cover are slidably connected in the two guide rails, a gear is rotationally installed in the center of the top end of the cover, and the output end of a first motor is fixedly installed at the top end of the gear. The ventilation quantity in the culture room can be accurately controlled through the cooperation of the ventilation holes in the cover plate arranged in the middle of the top end of the cover and the first movable cover and the second movable cover, then fine adjustment of humidity is achieved, and the optimal humidity environment needed by growth of antrodia camphorata is ensured.

The invention relates to the technical field of biological monitoring, in particular to a preparation method and application of a blood plate. The preparation method of the blood plate comprises the following steps: adding culture medium components into a culture medium, the culture medium components comprising peptone nitrogen, a yeast extract, reduced glutathione, sodium pyruvate and distilled water; adding blood into the culture medium; adjusting the pH value of the culture medium to be stable; adding a heat-sensitive component and a heat-stable component into the culture medium; and constructing a culture medium low-oxygen mixing environment. Therefore, the shelf life of the blood plate can be prolonged, and the hemolysis phenomenon is reduced.

This invention discloses a method for improving the high yield of extracellular polysaccharides from Bacillus belye on agar plates based on agricultural waste, belonging to the fields of microbial fermentation technology and resource utilization of agricultural waste. The method involves inoculating Bacillus belye seed culture into agar plates containing agricultural waste, culturing at 36-38°C to obtain fermentation products, and extracting the extracellular polysaccharides from the fermentation products. The agar plate containing agricultural waste comprises: peptone 9-11 g / L, yeast extract 4.5-5.5 g / L, NaCl 9.5-10.5 g / L, agar 17-19 g / L, agricultural waste powder 10-15 g / L, and pH 7.1-7.3. This method solves the problems of low yield, high cost, and high energy consumption associated with traditional liquid shake-flask culture, while simultaneously achieving high-value utilization of agricultural waste.

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The present disclosure provides systems and methods for detecting the presence of colonies on agar plates. The systems and methods are preferably useful for assessing the presence of contaminants (e.g., bacteria, fungi, etc.) in samples, such as raw materials or components of pharmaceutical compositions, by analyzing whether the sample forms colonies on an agar plate. The systems use lighting arrangements to enhance the accuracy of the analysis and, in embodiments, include the use of artificial intelligence as part of the automated process.

The invention discloses a giant strong aeromonas hydrophila bacteriophage and application thereof, and belongs to the technical field of microorganisms. According to the invention, aeromonas hydrophila is taken as host bacteria, the phage is enriched from a collected lake water sample, and is separated and purified by adopting a double-layer agar plate method, so that a strain of phage is successfully obtained, the strain of phage is named as aeromonas hydrophila phage Cgtyf, the preservation number is CCTCC NO: M 2026099, and the titer of the phage can reach 1.0 * 10 < 10 > PFU / mL; the genome size of the phage is about 237 kb, and the phage belongs to a giant phage. In an in-vitro bacteriostasis experiment, the bacteriophage Cgtyf shows a remarkable bactericidal effect and a relatively strong growth inhibition capability on host bacteria, and shows a relatively high temperature tolerance range in a temperature stability test. Therefore, the bacteriophage Cgtyf provided by the invention can be effectively used for preventing and controlling aeromonas hydrophila in aquatic products, has a potential application value as an antibacterial drug substitute, is beneficial to reducing or avoiding the use of antibacterial drugs in aquaculture, and has a good application prospect.

The invention discloses a purification method of buckwheat white mold pathogenic bacteria, which belongs to the technical field of biology, and comprises the following steps: S1, inoculating buckwheat white mold pathogenic bacteria spores on a PDA solid plate culture medium by using a sterilized brush or a single cell picking method, and culturing for 10 days; s2, punching the culture medium obtained in the step S1 by using a fungus cake puncher to obtain a fungus cake; s3, the fungus cakes obtained in the step S2 do circular motion on the edge of a new PDA culture medium to rub back and forth, so that white mold spores on the fungus cakes are rubbed to the culture medium, and culture is conducted. When the purification method disclosed by the invention is used for culturing the buckwheat white mold pathogenic bacteria, a large number of target strains after purification culture can be obtained about 10 days after a new PDA culture medium is inoculated with a bacterial cake.

The present invention relates to the field of biological bacteriophage technology, and discloses a bacteriophage for lysing Salmonella and Escherichia coli, and the bacteriophage is named Salmonella phage GRNPK. The bacteriophage is a virulent bacteriophage, has a strong lysis effect on both Salmonella and Escherichia coli, and belongs to the Demerecviridae family; the bacteriophage forms small and transparent plaques on a double-layer agar plate; has high stability, is active under pH 2-12 and under conditions of -20°C-70°C; has strong infectivity, and is cultured for 12 hours under the condition of an optimal multiplicity of infection (MOI) of 0.001, and has a titer of 1.1×10 12 PFU / mL. High-throughput sequencing showed that the phage GRNPK did not encode any lysogeny, toxin, drug resistance and virulence factor-related genes. In vitro experiments showed that GRNPK could effectively inhibit the growth of Salmonella and Escherichia coli and delay the bacteria from developing resistance to the phage. Animal test results showed that the phage could effectively reduce the number of Enteritidis Salmonella in the intestines of chicks.

The invention discloses a fish intestinal tract L-dopa decarboxylation strain as well as a separation, purification and identification method and application thereof, and belongs to the technical field of microorganisms. The bacterial strain is identified as enterococcus faecium SAGEF001, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20252372. The target strain is successfully screened from the scatophagus argus intestinal tract through BHI enrichment and PSE agar plate directional separation under the aerobic condition, and is identified as the fish intestinal tract L-dopa decarboxylated strain through morphology, molecular biology (16S rRNA gene V3-V4 region sequencing) and functionality (dopamine content detection), and a fish microorganism resource library can be enriched. The strain can efficiently decarboxylate L-dopa to generate dopamine, and can be used for preparing a microbial agent for aquaculture health regulation or environmental restoration.

The utility model discloses a bidirectional agar diffusion plate punching device which comprises a supporting assembly and a fixing assembly, and the fixing assembly is arranged on the upper end face of the supporting assembly. The supporting assembly comprises a plate part and an edge part, the fixing assembly comprises a fixing part and a clamping part, and the fixing part is detachably provided with a punching column. According to the utility model, the fixing component is clamped on the edge part of the supporting component through the clamping parts which are oppositely supported on the end surfaces of the two sides of the fixing part, the fixing part is provided with a plurality of groups of fixing holes, and a plurality of groups of punching columns are detachably fixed on the fixing part, so that the operation step of picking out the punched and removed agar by a needle head is omitted, and the integrity of the agar is effectively ensured; preparing an agar plate with a required pore size; threads are arranged in the fixing holes, threads are arranged in the middle of the punching column, and the height can be adjusted by rotating the punching column.

A bacterial colony-forming-unit (CFU) detection system is disclosed that exploits a thin-film-transistor (TFT)-based image sensor array that saves ˜12 hours compared to the Environmental Protection Agency (EPA)-approved methods. A lensfree imaging modality was built using the TFT image sensor with a sample field-of-view of ˜10 cm2. Time-lapse images of bacterial colonies cultured on chromogenic agar plates were automatically collected at 5-minute intervals. Two deep neural networks were used to detect and count the growing colonies and identify their species. When blindly tested with 265 colonies of E. coli and other coliform bacteria (i.e., Citrobacter and Klebsiella pneumoniae), the system reached an average CFU detection rate of 97.3% at 9 hours of incubation and an average recovery rate of 91.6% at ˜12 hours. This TFT-based sensor can be applied to various microbiological detection methods. The imaging field-of-view of this platform can be cost-effectively increased to >100 cm2.

The utility model discloses a traditional Chinese medicine sensitive agar plate puncher which comprises a base column, the base column is provided with a cylindrical hollow part, internal threads are arranged at the cylindrical hollow part, and a first screw is matched with the hollow part, so that the free end of the first screw can rotationally penetrate through the cylindrical hollow part to extend to the outside and is fixed through a nut; the plurality of brackets are uniformly distributed on the periphery of the foundation pillar; each bracket is provided with a rectangular hollow part, and the inner circumference of each rectangular hollow part is coated with a rubber gasket; the second screw is arranged at the rectangular hollow part, and the second screw is matched with the rectangular hollow part, so that the free end of the second screw can penetrate through the rectangular hollow part to extend to the outside and is fixed through a nut; and the second screw can translate or slide up and down in the rectangular hollow part. According to the hole puncher, the steps of bottom sealing and agar picking after punching are omitted, and meanwhile punched holes are uniform and attractive in distribution.

The utility model discloses a kind of excrement fungus microbial separation devices, including back type frame, the inside of back type frame is fixed with tray by supporting member, and the top end of the tray is arrayed with several circular lower grooves, and circular lower groove is placed with agar plate;C character sliding platform, the top end of back type frame is slidably installed in C character sliding platform, the top end of C character sliding platform is fixed with C character frame, and the both sides of C character frame inside are slidably installed with guide rod, the inside of C character frame is installed with the elastic down-pressing structure for forcing guide rod to go down.The utility model can help staff to quickly complete the regional division operation of strain, compared with each subarea, the device can handle multiple plates simultaneously, save a lot of time and labor cost of staff, and using the device can ensure that the partition position and size of each agar plate are consistent, can avoid error and inconsistency in manual operation.

The invention relates to a screening and fermentation regulation method of 5-hydroxytryptophan functional spore bacteria, which comprises the following steps: collecting abnormal fermentation bacterial liquid or bacterial residue waste, and suspending in a sulfuric acid aqueous solution with the pH value of 1.8-2.5 for 2 days; coating the suspension on a kanamycin resistance screening agar plate, and culturing at 30 DEG C for 48 hours; separating bacterial colonies on the resistant plate, carrying out shake-flask culture in a kanamycin resistant culture medium containing tryptophan, adding IPTG (isopropyl-beta-d-thiogalactoside) for induction in the later stage of index fermentation, detecting that tryptophan in fermentation liquor is converted into 5-hydroxytryptophan, and storing the 5-hydroxytryptophan in a refrigerator at the temperature of 80 DEG C below zero; the method comprises the following steps: resuscitating a strain, culturing and amplifying in a shake flask, transferring into a 5L fermentation tank, fermenting with a culture medium containing 20g / L tryptophan, adding IPTG (isopropyl-beta-d-thiogalactoside) at the later stage of exponential growth to induce transformation, and simultaneously adding cumaric acid and ferulic acid to regulate and control the fermentation rate and the metabolic process. The functional bacterium provided by the invention is high in 5-HTP conversion specificity, only a small amount of isomeride is generated, the isomeride can be easily removed in the purification process, and few polymers are generated in the strain fermentation process.

Disclosed herein is a polymeric nanoparticle comprising a polymeric material that is susceptible to enzymatic degradation and a dye encapsulated within the polymeric material. Also disclosed herein are microfluidic devices and plates of a solid medium (e.g. agar) and methods making use of said nanoparticles.

The invention discloses a modified activated carbon-microbial agent coupling body and a preparation method and application thereof, and belongs to the technical field of water environment pollution removal, and the method comprises the following steps: carbonizing dry coconut shell particles, and then activating to obtain activated carbon; modifying activated carbon to obtain modified activated carbon; in a sterile closed container, iso-butane is used as a unique carbon source and energy source, and rhodococcus rhodococcus is cultured on a minimum agar plate; putting the modified activated carbon on the minimum agar plate, and fully mixing the rhodococcus purpureus and the modified activated carbon to obtain a modified activated carbon-microbial agent coupling body; the modified activated carbon provided by the invention has rich porous structures and surface functional groups, provides efficient adsorption and enrichment of polycyclic aromatic hydrocarbons, effectively fixes rhodococcus purpureus capable of degrading polycyclic aromatic hydrocarbons, promotes colonization and growth of microorganisms by own carbon sources and trace elements, realizes green and efficient degradation of polycyclic aromatic hydrocarbons in water environment sediments, and has good application prospects. And the recovery of the river and lake ecosystem is accelerated.

The utility model relates to a big flow biological sol of adjustable flow sampling device, including air filter body and sampling mechanism, the upper end of air filter body is equipped with the air inlet, air filter body lower extreme is equipped with the air outlet, the air outlet is linked with the air pump, still be equipped with screen between the air inlet and air outlet, sampling mechanism sets up in the below of screen, sampling mechanism includes sampling cotton. The utility model can effectively solve the problem that the existing agar plate sampling device is in the long -time sampling process because of the airflow impact and leads to the culture medium water loss, cannot gather virus and the problem that the detection range is limited, and sampling efficiency is high, and sampling effect is good.

The application discloses a single-cell blue algae separation and purification method based on rotifer enrichment and application, and the method comprises the following steps: placing a single-cell blue algae solution into a sterile BG11 conical flask; adding a rotifer solution with a known concentration into the sterile BG11 conical flask containing the single-cell blue algae solution to obtain a mixed solution of the rotifer and the single-cell blue algae; placing the sterile BG11 conical flask containing the mixed solution of the rotifer and the single-cell blue algae in a first culture environment for culture to obtain a blue algae and rotifer enrichment solution; transferring the blue algae and rotifer enrichment solution to a sterilized BG11 agar plate for plate coating treatment, and inverting the BG11 agar plate in a second culture environment for culture until a blue algae colony without bacteria is formed; and expanding the blue algae colony without bacteria to obtain single-cell blue algae without bacteria. The application can greatly shorten the time for traditional separation and culture of single-cell blue algae, and can prevent bacterial pollution from an external environment.

This utility model discloses a plate punching device for antibacterial experiments, including a puncher body with a punching head for punching agar plates at one end; a push rod coaxially arranged with the punching head; and a push rod pressing part located at the other end of the puncher body. By pressing the push rod pressing part, the push rod moves along its own axis to push out the agar block stuck in the punching head. This utility model, by setting a push rod and a push rod pressing part coaxial with the punching head, allows the experimenter to simply press the push rod pressing part after punching to move the push rod along its own axis and push out the agar block stuck in the punching head. This allows the experimenter to complete the punching process more smoothly, reduces operation interruption time, improves the convenience and efficiency of agar block removal, and reduces the complexity of the experimental operation.

The invention discloses a bio-based oil displacement agent for improving recovery efficiency and a preparation method of the bio-based oil displacement agent. The preparation method comprises the following steps that bacillus subtilis BZ-1 is subjected to activation culture on an LB culture medium plate, the bacillus subtilis BZ-1 is preserved in the China General Microbiological Culture Collection Center on November 15, 2024, and the preservation number is CGMCC No.32653; the preparation method comprises the following steps: inoculating a single colony on a flat plate into an LB liquid culture medium for culturing, carrying out enlarged culture, then transferring to a fermentation culture medium, carrying out preliminary filtration, wall breaking treatment and fine filtration on the cultured bacterial liquid, and then compounding with decyl hydroxypropyl sulfobetaine and fatty alcohol-polyoxyethylene ether ammonium sulfate to obtain the bio-based oil displacement agent. The bio-based oil-displacing agent can effectively reduce surface tension and oil-water interfacial tension, has a remarkable oil-displacing effect, and can solve the problem of low oil recovery rate of low-permeability oil reservoirs.