Evaluation method for disinfection effect of disinfectant

A technology for evaluation methods and disinfection methods, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., and can solve the problems of no quick and convenient method for evaluating and detecting the effect of disinfectants, time-consuming and labor-intensive problems, etc.

Inactive Publication Date: 2012-01-18
LUOYANG HUIZHONG ANIMAL MEDICINE
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  • Description
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Problems solved by technology

As a method to evaluate the sterilization rate, the agar plate method has intuitive and clear results, but this method requires specific test conditions and professional operating skills. There is a lot of

Method used

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  • Evaluation method for disinfection effect of disinfectant
  • Evaluation method for disinfection effect of disinfectant
  • Evaluation method for disinfection effect of disinfectant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Detection of the sterilization effect of the aldehyde disinfectant glutaraldehyde solution on the surface of an empty pig house

[0042]Sample: the ground of the laboratory (pharmacology room) of the National Veterinary Drug Engineering Technology Research Center. Disinfectant: 25% glutaraldehyde solution, Chengdu Kelong Chemical Reagent Factory, batch number: 20090224. The specific operation method is as follows: take 1.56ml of 25% glutaraldehyde solution, add sterilized purified water to 50ml, prepare 0.78% (v / v) glutaraldehyde solution, dilute it to 100 times with sterilized physiological saline to obtain the concentration It is a 0.0078% (v / v) solution of glutaraldehyde. Take an area on the floor of the laboratory, divide it into three adjacent small squares of 5 mm × 5 mm with a ruler, and mark them as blank control group and 0.78% (v / v) glutaraldehyde disinfection group from left to right. , 0.0078% (v / v) glutaraldehyde disinfection group. Dip 10ml of...

Embodiment 2

[0048] Example 2 Detection of the disinfection effect of the quaternary ammonium salt disinfectant benzalkonium bromide solution on the flushing sewage of piggery

[0049] Sample: Sewage from the pig cages in the experimental animal room of the National Veterinary Drug Engineering Center after washing. The concentration of live bacteria counted by agar plate method was 5.96×10 6 / ml, put in a 4°C refrigerator for later use. Disinfectant: benzalkonium bromide content 45%, Chengdu Kehongda Industrial Development Co., Ltd., batch number: 0903060102. The specific operation method is as follows: get 1ml of 45% benzalkonium bromide solution and add 9ml of sterilized normal saline to make a 4.5% solution, get 100ul of 4.5% benzalkonium bromide solution and add 4.4ml of normal saline to obtain 0.1% Benzalkonium bromide solution 4.5ml. Another 10 ul of 4.5% benzalkonium bromide solution was taken, and 4.49 ml of sterilized physiological saline was added to prepare 4.5 ml of 0.01% be...

Embodiment 3

[0053] Example 3 Detection of surface bactericidal effect of disinfectant peracetic acid solution on air disinfection of piggery

[0054] Sample: Empty pig house that was not cleaned and disinfected in the animal room of the National Engineering Center. Disinfectant: peracetic acid, AR, content ≥ 15%, Yantai Shuangshuang Chemical Co., Ltd., batch number: 20090401. The specific operation method is as follows:

[0055] Sampling before disinfection: Take two areas on the surface of the pigsty, the wall and the ground respectively, draw two similar squares (diameter 5mm×5mm) in each area, and mark the two squares in the same area respectively for the control group and the experimental group. For the control group, samples were taken before disinfection, and 10ml of normal saline was dipped in a cotton swab to repeatedly scrub 3 sampling points, and then put into the water together with the cotton swab. After mixing liquid A and liquid B, place peracetic acid at room temperature...

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Abstract

The invention provides an evaluation method for a disinfection effect of a disinfectant with a bacteria test sheet. The method comprises the following steps of: (1) preparing and grouping samples to be tested; (2) disinfecting: uniformly mixing the samples to be tested and diluted disinfectant and disinfecting; (3) inoculating: inoculating the samples to be tested and the disinfected samples on a total bacterial colony number test sheet; (4) culturing: packaging the bacterial colony test sheet on which the samples are inoculated into a sealed bag and placing in a thermostat of 37 DEG C to culture for 24-48 hours; and (5) observing a result: counting the bacteria number on each test sheet and calculating sterilization rate of each disinfectant. The evaluation method for the disinfection effect of the disinfectant with the bacteria test sheet is simple and convenient, has high accuracy and is an effective method which can substitute the conventional agar plate sterilization rate evaluation method.

Description

technical field [0001] The invention relates to an evaluation method of the disinfection effect of a disinfectant, in particular to an evaluation method of the disinfection effect of a disinfectant applied to a breeding place. Background technique [0002] The current evaluation of the effect of disinfectants can only be carried out in the laboratory or in a specific environment. It is mainly measured by the inactivation rate of viruses and the bactericidal rate of bacteria. Viruses can only grow on poultry embryos or cells, and the cultivation requirements are relatively high. , and the results are not easy to observe, so the killing effect on bacteria is an important indicator for evaluating the disinfection effect of disinfectants. The killing effect of disinfectants on bacteria is mainly reflected in the killing rate or killing index of bacteria. (1992) No. 101 notice of Nong (Husbandry Medicine) pointed out that the disinfection effect of the disinfectant registered in...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 张许科刘兴金张晓会
Owner LUOYANG HUIZHONG ANIMAL MEDICINE
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