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Bacilluscereus and application thereof

A technology of Bacillus cereus and application, which is applied in the direction of application, bacteria, fungicide, etc., can solve the problems of being easily affected by external conditions and difficult colonization, etc., and achieves easy cultivation and preservation, wide adaptability and tolerance strong effect

Inactive Publication Date: 2013-05-08
BIJIE COMPANY OF GUIZHOU TOBACCO +1
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the general existence of bio-control bacteria is not easy to colonize, and is easily affected by external conditions, such as being sensitive to light, ultraviolet rays, and drying.

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0023] Bacillus cereus 1205 ( Bacillus cereus 1205) separation steps:

[0024] (1) Tested strain: Phytophthora nicotiana ( Phytophthora nicotianae ), isolated, screened, identified, and preserved in the Microbiology Laboratory of Guizhou University.

[0025] (2) Isolation and purification of antagonistic bacteria: Weigh 10g of tobacco planting soil samples collected from Dafang County, Bijie City, Guizhou Province, into 90mL of sterile water with glass beads, shake at 160rpm on a shaker for 20min, let stand for 5min, absorb the soil to suspend The solution was diluted step by step with sterile water. Draw 0.1mL diluent to coat beef extract peptone (5.0g beef extract, 10.0g peptone, 5.0g NaCl, 20g agar, 1L water, pH7.2, 121°C, sterilize for 45min) plate, incubate at 37°C for 36h, wait After colonies grow on the plate, single colonies are picked and purified, and the numbers are stored for testing.

[0026] (3) Screening of antagonistic bacteria: Use plate confrontation m...

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Abstract

The invention discloses a bacilluscereus (Bacilluscereus1205) and an application thereof. The morphological characteristics are as follows: the 1205 bacterial strain is cultivated on an ordinary agar plate culture medium at 37 DEG C for 36 hours, the bacterial colony is nearly round, grey white, non-transparent and as large as 3-7mm, the edge is irregular and expanded, the surface is rough, granulated and is like ground glass or wax, no pigment is secreted; the bacilluscereus is a gram positive bacteria, rod-shaped, 1.0-1.3*3.0-5.0 micrometer, chaining and moveable; the spore is ellipse, terminal or secondary terminal and unclearly expanded; and the bacilluscereus can endure the cultivation of 7% NaCl. The bacilluscereus has the characteristics of strong stress resistance, simple nutritional requirement, stabilization, easy colonization, stability to light and ultraviolet, low cost, long storage period, is easy to apply and realize industrialization, and has a certain growth promoting effect on tobaccos.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacillus cereus strain and the use of the bacillus cereus strain in inhibiting tobacco black shank. [0002] Background technique [0003] Tobacco is an important economic crop and one of the crops with the most serious disease damage. From sowing to harvesting, the whole process can be infected by pathogens and cause invasive diseases. Tobacco black shank ( Phytophthora parasitica var nicotianae (Breda de Hean) Tuker) is one of the most harmful diseases in tobacco production. In addition to the sporadic occurrence in the northeast tobacco-growing areas in my country, it occurs widely in South China, Central China, East China and Northwest China, including Taiwan Province, and the economic loss is relatively large. serious. [0004] At present, disease-resistant varieties, cultivation control, chemical control, and biological control measures are generally used in production. How...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/085
Inventor 张传萍李祝葛永怡黄化刚申燕胡玮高冬冬张龙林
Owner BIJIE COMPANY OF GUIZHOU TOBACCO
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