83 results about "Horse blood agar" patented technology

An elastomeric article having reducing microbe affinity and transmission and methods for applying and immobilizing antimicrobial compounds to the elastomeric substrate surface are disclosed. The elastomeric article has a body formed of a natural or synthetic polymer latex having an outer surface and an inner surface. The body has a coating of an antimicrobial agent over at least a portion of said outer surface. The treatment involves applying according to either a spraying or dipping process an antimicrobial polymer or composition to a surface of the elastomeric substrate; binding the antimicrobial composition to the surface in a manner such that said treat antimicrobial coating passes either one or another or both versions of a zone of inhibition test, such test including: a) a dry-leaching or agar-plate-based contact test, according to AATCC 147 protocol, or b) a wet-leaching or dynamic shake flask test according to ASTM E-2149-01 protocol. The substrate is further subject to a rapid germicidal contact-transfer test of relatively short duration. The antimicrobial polymer can include an organosilane quaternary ammonium or a biguanide compound which can disrupt the ionic charges of microbial cellular membranes.

The invention discloses a manufacture method for rhamnolipid that includes the following steps: filtering fungus, inoculating into culture medium after taking dipping preprocess to the sample getting from oil field sewage or grease, inoculating on blood agar plate for culturing, selecting single colony, inoculating on blue agar plate for culturing, selecting the colony having blue halo to the bevel for microbial conserving; fermenting the primary screening strain in fermenting culture medium, testing the oil drainage activity, emulsibility and fermenting liquid surface tension, selecting target strain; taking ferment to the selected strain, and optimizing the fermenting culture medium, fermenting condition and strain to gain rhamnolipid fermenting liquid; distilling rhanmolipid raw product from the rhamnolipid fermenting liquid. The invention has high yield, good heavy hydrocarbon degradation effect, and improves the selecting accuracy.

A process is described for the production of an immunostimulant by submerged cultivation of Lentinus edodes in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.

The invention provides a Korean pseudomonas and application thereof. The Korean pseudomonas is identified as Korean pseudomonas CJ-361, and the preservation number is CGMCC NO.12122. The Korean pseudomonas CJ-361 is obtained through separation from radix pseudostellariae rhizosphere soil by adopting a pseudomonas selectivity culture medium, an antagonistic experiment is performed on fusarium oxysporum and fusarium moniliforme separated to the radix pseudostellariae root lesion portion through an agar plate opposite culture method, and it is found that the Korean pseudomonas has the strong antagonism on the fusarium oxysporum and the fusarium moniliforme. Meanwhile, the invention provides the optimum culture medium for antagonistic bacterium growth, the optimum formula is 1/4LB+1/20MS (containing no ferric salt)+0.1 wt% brown sugar, and the fermented liquor has the obvious antagonistic effect. The antagonistic strain and the microbial agent thereof can effectively prevent and treat radix pseudostellariae root rot diseases, and the environment-friendly, ecological and safe biological prevention and control potential strain is provided for overcoming or relieving replantation diseases of radix pseudostellariae.

The invention discloses a bacilluscereus (Bacilluscereus1205) and an application thereof. The morphological characteristics are as follows: the 1205 bacterial strain is cultivated on an ordinary agar plate culture medium at 37 DEG C for 36 hours, the bacterial colony is nearly round, grey white, non-transparent and as large as 3-7mm, the edge is irregular and expanded, the surface is rough, granulated and is like ground glass or wax, no pigment is secreted; the bacilluscereus is a gram positive bacteria, rod-shaped, 1.0-1.3*3.0-5.0 micrometer, chaining and moveable; the spore is ellipse, terminal or secondary terminal and unclearly expanded; and the bacilluscereus can endure the cultivation of 7% NaCl. The bacilluscereus has the characteristics of strong stress resistance, simple nutritional requirement, stabilization, easy colonization, stability to light and ultraviolet, low cost, long storage period, is easy to apply and realize industrialization, and has a certain growth promoting effect on tobaccos.

The invention provides a method and a kit for identifying Vibrio harveyi quickly by means of strong vibrio harveyi phage and belongs to the technical field of identification of cultures of Vibrio harveyi. The method comprises the following steps: 1) coating 10<7>-10<9>cfu/mL to-be-detected culture to a LB agar plate, leaving the LB agar plate to stand for 15-20min to obtain a to-be-detected plate;2) dropwise adding a strong vibrio harveyi phage mixed liquid to the to-be-detected plate to obtain an identification system; and 3) leaving the identification system obtained in the step 2) to standfor 8-16h at 28-35 DEG C, observing whether bacteriophage plaques appear or not in the identification system, and identifying the to-be-detected culture as the Vibrio harveyi. The method is low in cost and time- and labor-saving, the time needed is only 16-18h, and the method is particularly suitable for initially screening a lot of Vibrio harveyi.

The invention provides a blood agar plate, and relates to the technical field of culture media. The blood agar plate contains a plurality of substances such as casein tryptone and soy peptone. The casein tryptone, the soy peptone, yeast extract powder and beef heart infusion provide suitable carbon and nitrogen sources for bacteria; defibrillated sheep blood is rich in nutritional factors to promote the growth of common bacteria, and at the same time the hemolysis of the bacteria can be observed; sodium chloride maintains the osmotic pressure balance of the bacteria; and agar is a culture medium coagulant. The blood agar plate has more comprehensive nutrients, can effectively promote the growth of the bacteria, and improve the isolation rate of the bacteria; according to a preparation method of the blood agar plate, after a culture solution is sterilized at high temperature, cooling is performed to 80-90 DEG C, so that full gas discharging time is given to the culture solution to avoidformation of air bubbles during rapid cooling, and at the same time, before adding the defibrillated sheep blood, full preheating is performed to avoid the problem of agar agglomeration caused by toolarge temperature difference between the defibrillated sheep blood and the culture solution; and the method has simple operation and low requirements for equipment, and is suitable for industrial production.

The invention discloses an efficient calcium mineralizing bacterium and an application thereof, and belongs to the field of microorganisms. The bacterium disclosed by the invention is bacillus cereusEMCa1. The strain is in a short rod shape, and is in a short or long chain, the size of the bacterium is about (1.0-1.2) x (3.0-5.0) [mu]m, the strain is a gram staining positive bacterium, white circular bacterial colonies appear after the strain is cultured for 24 h on an LB agar plate culture medium at 30 DEG C, a diameter is about 5-7 mm, the edge is irregular, and the bacterial colony surfaceis like wax. The induced calcium carbonate deposition characteristic of the strain is as follows: after 20 days of culture, the strain can convert free calcium ions in a coral sand solution into a calcium carbonate precipitate, and in a calcium carbonate deposition experiment, the concentration of calcium ions is reduced from 36.9 mg/L to 7.19 mg/L, and the proportion of calcite in coral sand isincreased from 2% to 20%. According to the efficient calcium mineralizing bacterium and the application thereof, calcium ions can be mineralized to generate calcium carbonate, so that the efficient calcium mineralizing bacterium is applied to the restoration of stone cultural relics.

The invention specifically discloses a test method for evaluating the in-vitro antibacterial activity of an antibacterial substance, belonging to the technical field of biological control. According to the method, a methylene blue agar plate containing bacteria is used for agar-plate-diffusion-process in-vitro antibacterial activity test. The method is visual and simple, realizes evaluation through observation of a colored inhibition zone and overcomes the disadvantage of difficulty in accurate measurement of the diameter of an inhibition zone due to deep and turbid colors and complex components during in-vitro antibacterial test of traditional Chinese medicines or other to-be-measured substances with similar properties by using traditional agar plate diffusion processes.

The invention discloses a method for quantitatively detecting the activity of L-amino acid oxidase by using a Prussian blue agar plate, which comprises the following steps: with a punch, punching a small hole having a diameter of 4-10 mm on the Prussian blue agar plate; then, adding a liquid to be determined into the small hole, and standing at room temperature for 10-30 minutes, wherein hydrogen peroxide generated by the liquid to be determined forms a blue ring around the small hole; and determining the diameter of the blue ring, determining the release amount of hydrogen peroxide according to the hydrogen peroxide and a standard straight line made of the diameter of the blue ring, and then deducing to obtain the activity of L-amino acid oxidase, wherein the liquid to be determined is a reaction liquid obtained by reacting an enzyme source and a substrate under the conditions of a temperature of 25-50 DEG C and a pH value of 5-9 for 0.5-2 hours, the enzyme source is an enzyme obtained through the fermentation culture of Pseudoalteromonas sp. B3, and the substrate is L-amino acid. The method has the characteristics of low cost, simple and convenient operation process, wide applicability, high stability and the like.

The invention discloses a recombinant Pichia pastoris culture medium, and a culture method and application thereof. The recombinant Pichia pastoris culture medium is prepared from cheap glucose, corn steep liquor, partial inorganic salts and a small amount of yeast extract. The recombinant Pichia pastoris culture medium is convenient to prepare and suitable for industrial production. The glycerol strain is subjected to bleomycin-containing YPD agar plate rejuvenation and sequentially subjected to seed culture, fermentation tank early-stage culture and fermentation tank later-stage fed-batch nutrient solution culture; and after the recombinant Pichia pastoris is cultured for 18-24 hours, the diluted coating culture counting detection indicates that the yeast living cell number in the fermentation liquid can reach 4 billion/mL or above, the wet bacterium weight can reach 180-220 g/L, and the glucose-to-yeast yield is 0.43-0.46 which is approximate to the theoretical value 0.5. The concentration of the metabolism byproduct ethanol in the whole culture process is almost 0, and the bacterium concentration and wet bacterium weight can quickly increase to the target values, thereby effectively shortening the culture period, enhancing the equipment utilization rate and lowering the production cost.

An improved method of preparing a bdellovibrio bacteriovorus preparation is disclosed. Through repeated researching and experiments by inventors, a bdellovibrio bacteriovorus plaque obtained by screening adopting a two-layer tap water agar plate is picked and added into 200 mL of a host bacterium suspension, then the mixture is subjected to shake cultivation at 28 DEG C for 90 h, with a speed being 150 rpm, and an obtained liquid mixture is adopted as a bdellovibrio bacteriovorus suspension. The bdellovibrio bacteriovorus suspension can be directly used for tests. The whole preparing process is simple and rapid, thus saving time and the cost.

The invention discloses a beer yeast strain and an application thereof in beer fermentation. The invention provides a saccharomyces cerevisiae Z5 with a preservation number of CGMCC No 3218. A breeding method of the yeast strain comprises the following steps: preparation of an original parent strain, activation in a test tube, ion implantation mutagenesis, laser mutagenesis, wort agar plate primary screening, fermentation bung secondary screening, passage stability test and pilot test. The yeast strain is an excellent beer yeast strain and has a potential of large-scale production of beer breweries.

The invention discloses vibrio marinopraesens and a separation method thereof. Vibrio marinopraesens HN897 generating agarase is separated and obtained from seawater samples of coastal areas in SouthChina, and is preserved in China General Microbiological Culture Collection Center on August 8, 2018 with the preservation number of CGMCC NO.16232. Through species identification, the vibrio marinopraesens HN897 is shown as Vibrio astriarenae. The bacterial strain cultured on a solid agar plate shows that the bacterial strain has the capacity for degrading agar, and the activity of agarase secreted from 2 culturing liquid (TSB and LB) shows that the bacterial strain has high-yield capacity of agarase. Therefore, the vibrio marinopraesens HN897 has wide application and market prospects in thebiopharmaceutical fields of agaro-oligosaccharides and the like, and besides, establishes a basis for subsequent preparation of enzyme products being high in vitality and high in purity.

The invention discloses a vacuum membrane filtering and microorganism culturing device, which relates to an analyzing filtration and microorganism culture device. The invention applies an electric diaphragm pump as the vacuum source; the liquid medium is absorbed on the cellulose liner which is positioned below the micro-porous membrane through the vacuum membrane filtering method; the membrane filtering device is refitted into a culture dish; and the device is cleaned and sterilized automatically with the cleansing detergent and ozone. The device is a mechanical and electrical integration device, which comprises the membrane filtering device (1), a filtrate receiver (2), the electric diaphragm pump (3), an air-in electromagnetic valve (4), a liquid-in electromagnetic valve (5), an ozone generator (6), a pressure sensor (7), a liquid crystal display (8), a micro-control system (9), a solution bottle (10) and a drain pipe (11). The invention has convenient application and strong practicality; the invention avoids the busy work of dumping filtrate, transferring membrane, cleaning and sterilizing and preparing agar plate; the invention also has the characteristics of intelligence, integration and multi-function; the invention suits for filtration technology areas such as medicine and health etc.

The invention discloses a separating and screening method of a source sludge reduction strain. The separating and screening method of the source sludge reduction strain comprises separation of strainsand further screening of the strains, and comprises the following steps: taking fresh sludge of a sewage treatment plant, loading the fresh sludge of the sewage treatment plant into a glass container, conducting sealing, and conducting shaking culture to obtain acclimated activated sludge; coating a sludge-containing sterile agar plate culture medium with supernate, subjected to gradient dilution, of the activated sludge, and after inverted culture is conducted, selecting strains, with transparent circles, around colonies for plate streaking purification to obtain purified bacteria; and taking the separated purified bacteria, activating the separated purified bacteria on LB culture media respectively, taking the strains at the same growth stage, adding the strains at the same growth stageinto glass containers containing sludge with a certain concentration with adding amounts controlled to be the same, carrying out a shake test at a normal temperature, conducting sampling at a fixed interval, comparing obtained samples and a sample without strains, recording data, and conducting plotting according to the data to obtain a dominant strain. According to the dominant strain screened out by means of the separating and screening method of the source sludge reduction strain, without any optimization treatment, the largest sludge removal rate is higher than that of the blank sample by12.2%.

The invention belongs to the technical field of biology and medicine, and relates to drug susceptibility tests for important antibacterial agents, in particular to a method for restoring the antibacterial activity of tigecycline, aiming to achieve accuracy of tigecycline susceptibility results detected by in-vitro conventional drug susceptibility tests. The method has the advantages that a stabilizing agent is applied to eliminate influence factors in the tigecycline susceptibility tests, the tigecycline restores the susceptibility to pathogenic bacteria, and a simple method for accurately measuring the susceptibility of the tigecycline to the pathogenic bacteria is created; addition of other reagents is not needed, an appropriate volume of resensitization liquid is added into tigecyclinepaper sheets, MHA agar plates or microporous plates on the basis of normal operations, and accordingly, major problems in the tigecycline susceptibility tests in the prior art, such as false drug resistance, can be solved; the method is simple and convenient to operate, has accurate results and high repeatability, and the like.

The invention provides a preparation method for cephalosporin C acylase, which belongs to the field of biochemistry. The method comprises the following steps: 1) plate cultivation: a step of coating an LB agar plate medium with a cephalosporin C acylase producing strain genetically engineered Escherichia coli strain CPCA2013 and carrying out cultivation; 2) seed cultivation: a step of selecting and picking a single colony from the LB agar plate medium, inoculating the single colony into a seed medium and carrying out seed cultivation; and 3) ferment cultivation: a step of inoculating a seed liquid obtained after seed cultivation into a fermentation medium for cultivation, wherein the inoculation amount of the seed liquid is 3 to 4% of the volume of the fermentation medium. According to the cephalosporin C acylase produced in the invention, the enzyme activity of fermentation broth is about 5000 U/L, and large-scale industrial production of the cephalosporin C acylase may be carried out according to results of cost accounting; and the enzyme activity of the fermentation broth is 0.8 time higher compared with the enzyme activity of fermentation broth produced in the prior art, enzymatic hydrolysis effect is good, and enzyme activity stability is high.

The invention discloses a method for detecting anti-mold performance of a silkworm excrement product. The method comprises a first step of performing streak inoculation on a nutrient agar bevel, so as to serve as bevel preservation fungi; a second step of pouring sterile water into bevel strains cultivated well in the first step, washing spore liquid and pouring the spore liquid into a sterile triangular flask containing glass beads, and obtaining fungal spore liquid; a third step of centrifuging the fungal spore liquid, removing supernatant, adding sterile water to perform centrifugal washing, and preparing inoculating fungal spore liquid; a fourth step of manufacturing sample adding bags; a fifth step of adding the sample adding bags into sterile water to undergo pre-wetting to serve as blank reference samples, respectively adding anti-bacterial and non-anti-bacterial silkworm excrement products into the sample adding bags, adding sterile water for pre-wetting to serve as a to-be-tested sample and a contrast sample; a sixth step of respectively placing the to-be-tested sample, the contrast sample and a blank contrast sample on the surfaces of agar plates coated with the inoculating fungal spore liquid, and taking and adding the inoculating fungal spore liquid dropwise to the three samples; and a seventh step of recording the anti-mold performance of the silkworm excrement product. The method is simple to operate, quick in speed and low in using cost.