The invention discloses a separating and
screening method of a source
sludge reduction strain. The separating and
screening method of the source
sludge reduction strain comprises separation of strainsand further screening of the strains, and comprises the following steps: taking fresh
sludge of a
sewage treatment
plant, loading the fresh sludge of the
sewage treatment
plant into a
glass container, conducting sealing, and conducting shaking culture to obtain acclimated
activated sludge;
coating a sludge-containing sterile
agar plate culture medium with supernate, subjected to gradient
dilution, of the
activated sludge, and after inverted culture is conducted, selecting strains, with transparent circles, around colonies for plate streaking purification to obtain purified
bacteria; and taking the separated purified
bacteria, activating the separated purified
bacteria on LB culture media respectively, taking the strains at the same growth stage, adding the strains at the same growth stageinto glass containers containing sludge with a certain concentration with adding amounts controlled to be the same, carrying out a shake test at a normal temperature, conducting sampling at a
fixed interval, comparing obtained samples and a sample without strains, recording data, and conducting plotting according to the data to obtain a dominant strain. According to the dominant strain screened out by means of the separating and
screening method of the source sludge reduction strain, without any optimization treatment, the largest sludge removal rate is higher than that of the blank sample by12.2%.