The invention discloses a medical instrument disinfectant. The medical instrument disinfectant is prepared from, by weight, 1-2 parts of dibromohydantoin, 1.6-3.8 parts of paraformaldehyde, 11-15 parts of chervil larkspur herb, 7-13 parts of a disinfecting drug, 8-12 parts of folium baeckeae, 6-8 parts of leaves of eucalyptus tereticornis, 4-7 parts of whiteflower cacalia seeds, 2.2-3.8 parts of ethylene oxide, 2-3 parts of Gemini quaternary ammonium salt and 0.5-1.5 parts of chlorinated sodium phosphate. The medical instrument disinfectant has the advantages that multiple Chinese herbal medicines with the higher antibacterial effect are screened out for reasonable compatibility, and then the disinfectant which is capable of quickly killing bacteria, wide in antibacterial spectrum, long in bacteriostatic time and the like is prepared; the prepared disinfectant is good in disinfection effect, capable of quickly killing pathogenic bacteria on an instrument, free of corrosive action on the equipment, stable in performance and long in quality guarantee period.