31results about How to "No antibacterial effect" patented technology

The invention discloses a paper mulberry flavone nanometer preparation and a preparing method thereof. The preparing method includes: dissolving paper mulberry flavone powder with ethanol, and adding SDS to prepare an organic phase; dissolving chitosan with an acetic acid solution having a concentration of 2 wt% and adjusting the pH to 5.3-5.4 to prepare a water phase; adding an aqueous tween-80 solution into the water phase; injecting the organic phase into the water phase under high-speed stirring; adding an aqueous PEG 400 solution; stirring continuously for 30-60 min; and allowing the mixture to stand and be defoamed to obtain a suspension namely the paper mulberry flavone nanometer preparation. Operations are simple and equipment requirements are low. The main raw materials are natural biomolecules, and are good in biocompatibility, nontoxic, biodegradable, free of in-vivo accumulation, simple, easily available and low in cost. After nanocrystallization, the water solubility of the flavones is improved and the oral absorption efficiency is increased.

The invention provides a sea-buckthorn seed polypeptide solution with an inhibiting effect on intestinal pathogens as well as a preparation method and application of the sea-buckthorn seed polypeptide solution. The preparation steps are as follows: after sea-buckthorn seed residues are soaked, centrifugation is performed after ProteAX protease is added for hydrolysis, and the supernate is obtained, and is subjected to concentration and separation by an ultrafiltration membrane with the interception aperture of 5000 Da, so that the sea-buckthorn seed anti-bacteria polypeptide solution is obtained. The anti-bacteria culture experiment results show that the polypeptide solution within the concentration range of 10-20 mg / ml has the obvious inhibiting effect on salmonella, staphylococcus aureus, pseudomonas aeruginosa and other common intestinal pathogens. The sea-buckthorn seed polypeptide can be used as a feed additive.

The invention discloses veterinary yeast glucan injection, and aims to provide an animal immunopotentiator, which is prepared from yeast glucan through chemical modification and is a purely natural green veterinary medicine product. Each 100ml of injection contains 2 to 24g of sulfated or carboxymethyl water-soluble yeast glucan and the balance of water for injection. The injection can nonspecifically enhance the immunity of animals such as rabbits, pigs, sheep, cows and the like and improve the anti-infection capability of the animals. The preparation prepared by the invention is injection and has the advantages of no toxic or side effects, no residues and the like, is convenient to use and meets the current development requirements of green products.

The invention discloses recombinant bacteria for expressing plectasin and an application thereof. The classification name of the recombinant bacteria is lactococcus lactis NZ9700-pGMN3, the preservation serial number of the recombinant bacteria is CCTCC NO: M2014611, the recombinant bacteria can achieve upper-high expression of plectasin protein, the expression quantity of the recombinant bacteria far exceeds that of other anode recombinant bacteria, and the recombinant bacteria can be directly used for preparing anti-bacteria medicament, have no hidden safety risks and hardly have any side effects. The recombinant bacteria have the functions of inhibition of methicillin-resistant staphylococcus aureus, streptococcus agalactiae, streptococcus pyogenes, enterococcus faecalis and the like. Bacterial strains of the recombinant bacteria can achieve efficient and continuous expression under self-induction, the recombinant bacteria can express and secrete fusion protein containing plectasin in the fermentation process, the total yield of protein is more than 1.41g / L, plectasin accounts for more than 70%, and the recombinant bacteria have the potential of treating mammitis and infectious diseases of a genital tract.

The invention belongs to the field of antibacterial materials, and discloses a slow-release antibacterial molecular cluster resin, a preparation method and application thereof. Add aminopolystyrene microspheres and polyacrylic acid to ethanol solution, stir and reflux reaction under inert atmosphere, the product is washed and dried to obtain PS-PAA molecular cluster resin; then mixed with silver nitrate solution, adding reducing agent to react, the The silver that has been grafted on the PS-PAA molecular cluster resin is reduced to obtain nano-silver, and a slow-release antibacterial molecular cluster resin is obtained. According to the invention, the dendritic active group in the molecular cluster resin is closely combined with the nanometer silver, and the obtained product has excellent antibacterial properties and slow-release properties, and can be used for antibacterial in drinking water.

A kind of preservation solution of animal tissue and cell under low temperature of the present invention and using method thereof, use one or more of water, polyalcohol, Collin's solution, UW solution, HTK solution, Euro-collin's solution As a basic solution, polyphenols, citric acid, poloxamer and vitamin E are added, and the wavelength is in the range of 20KHz‑1MHz and the sound intensity is not greater than 3W / cm 2 Ultrasonic irradiation treatment can improve the viability and biological activity of animal tissues and cells in a low temperature environment by adjusting the ratio of different components in the preservation solution, and controlling the wavelength, sound intensity and irradiation time of ultrasonic waves.

The invention discloses a pseudomonas strain and application thereof. The strain is pseudomonas protegens IAS03, and is preserved in the China center for type culture collection on November 27th, 2015, and the preservation number is CCTCC NO:M 2015711. The strain is screened from pond bottom sludge and then a backwater body is used, so that ecological safety risks do not exist, and biological safety regulations are conformed. The strain has the specific antimicrobial function on aeromonas hydrophila and aeromonas sobria, has no inhibition effect on other bacteria and has no pathogenicity on fishes. Only one strain can be used for inhibiting aeromonas hydrophila and / or aeromonas sobria. Compared with traditional antibiotic and sterilizing agent prevention measures, the strain has the advantages of being safe, environmentally friendly and efficient. The strain can be developed into a microecological preparation to control aeromonas sobria and aeromonas hydrophila in the breeding environment and prevent hemorrhagic septicemia caused when the fishes are infected with aeromonas sobria and aeromonas hydrophila.