A kind of recombinant bacteria expressing plectasin and its application
A technology for the function of plectasiamycin, which is applied to recombinant bacteria expressing plectasiamycin and its application field, which can solve the problems of increasing the difficulty of curing diseases, patients and social and economic burdens, and achieve the effect of increasing expression
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[0021] Example 1: Construction method of recombinant Lactococcus lactis expressing plectasin
[0022] 1. Construction of recombinant strains
[0023] The nucleotide sequence of the Usp45 protein signal peptide was cloned from Lactococcus lactis MG1363, and the Usp45 gene fragment was combined with the shuttle plasmid pMG36e (the pMG36e map is shown in figure 1 shown) to construct plasmid pGMN1; connect the plasmid pGMN1 with the Pnis-MCS segment gene amplified from the lactic acid bacteria plasmid pNZ8408 to construct the plasmid pGMN2; artificially synthesized lactic acid bacteria codon-biased plectasin gene fragment, the optimized strain The base sequence of silk mycin is: GGT TTT GGT TGT AAT GGT CCATGG GAT GAA GAT GAT ATG CAA TGT CAT AAT CAT TGT AAA TCA ATT AAA GGT TAT AAAGGT GGT TAT TGT GCT AAA GGT GGT TTT GTT TGT AAA TGT TAT (SEQ ID NO : 1), the optimized plectasin gene fragment was connected to the plasmid pGMN2 to obtain the plasmid pGMN3, in which Usp45 was located u...
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