6 results about "Plectasin" patented technology

The invention discloses an antibacterial peptide PPI45 / PPI47 as well as a preparation method and application of the antibacterial peptide PPI45 / PPI47. According to the invention, fungal defensin Plectasin is used as a template, and the self-assembled antibacterial peptide PPI45 / PPI47 is designed. The expression of the self-assembled antibacterial peptide PPI45 / PPI47 in pichia pastoris is realized by optimizing the gene sequence of the antibacterial peptide and constructing a specific expression vector, a perfect purification system is established, large-scale production can be realized, and the self-assembled antibacterial peptide PPI45 / PPI47 can be applied to the fields of antibacterial drug development, hydrogel wound treatment and the like, and has wide application value and market prospect.

The invention discloses a drug-loaded nanoparticle as well as a preparation method and application thereof. The drug-loaded nanoparticle can be used for treating triple negative breast cancer, and specifically comprises a magnetic nanoparticle, an active component loaded in the magnetic nanoparticle, and an erythrocyte membrane coating the magnetic nanoparticle, wherein the active ingredients are selected from one or more of piperlongumine, polyphyllin II, erianin, pseudolaric acid, 6-gingerol, ginkgetin, solasonine, cucurbitacine B and lycorine. In practical application, the drug-loaded nanoparticles have good biological safety, long circulation capability and high tumor retention capability, and can generate efficient killing and treatment effects on triple-negative breast cancer by inducing cell apoptosis.

The invention provides a functional feed for promoting growth of channel catfish. The functional feed consists of a pellet feed and plectasin peptide, the pellet feed comprises the following raw materials in parts by weight: 22-25 parts of imported fish meal, 1-3 parts of mutton fat, 14-16 parts of euphausia superba powder, 1.5-2.5 parts of soybean oil, 10-12 parts of meat and bone meal, 1-2 parts of corn oil, 11-12 parts of soybean meal, 10-11 parts of wheat middling, 7.4-8.4 parts of peanut kernel meal, 1-2 parts of a feed adhesive, 4-6 parts of brewer's grains, 0.8 part of a compound immunopotentiator, 4-5 parts of fish oil, a primary compound phagostimulant and 2 parts of euphausia superba oil. 0.8 part of a multi-vitamin mixture, 0.9 part of lard oil and 0.6 part of a multi-mineral mixture; the proportion content of the plectasin peptide is 0.5 to 5 parts. The plectasin peptide is added into the channel catfish feed, so that the growth speed of the channel catfish can be effectively increased, the feed coefficient of the feed is reduced, the breeding period of the channel catfish is shortened, and the breeding benefit of the channel catfish is increased.

The invention provides a tissue-type spheroid culture medium as well as a preparation method and application thereof. The culture medium comprises a basic culture medium, a plant-derived recombinant human serum albumin OsrHSA, a growth factor FGF-10, a low-oxygen simulation agent CoCl2 microcapsule, an inhibitor Rhosin, an antioxidant NAC, an antibiotic Plectasin, a targeted substitute peptide Tarextumab and a self-assembled scaffold (CultrexBME and / or RADA16-IKVAV), and the preparation method of the culture medium comprises the following steps: integrating thermosensitive components (a plant protein module, the inhibitor and the antibiotic) through a freeze-drying functional ball technology; and redissolving and assembling with a basic culture medium, and combining with a low-oxygen simulation agent microcapsule sustained-release system to form a gel-state culture medium. The culture medium is suitable for co-culture of various tumor cells and construction of an organoid model, and can accurately simulate a tumor microenvironment and improve the drug screening reliability.

This invention relates to the field of antimicrobial peptide technology, and particularly to the antimicrobial peptide Ple-AB, its preparation method, and its applications. Using the fungal defensin Plectasin as a template, this invention designs and modifies the antimicrobial peptide Ple-AB. Compared with Plectasin, this antimicrobial peptide exhibits significantly higher antimicrobial activity and better bactericidal effects against Gram-positive bacteria; it also shows higher stability, particularly excellent resistance to trypsin; and its fermentation expression level is significantly improved. Furthermore, this antimicrobial peptide has very low hemolytic activity and cytotoxicity, making it suitable for development in antimicrobial drugs, additives, cosmetics, health products, and other fields, with broad application value and market prospects.

The invention provides an antiviral composition containing oryzanol and ningnanmycin and application of the antiviral composition, and belongs to the technical field of biopesticides. The active ingredients of the antiviral composition are ningnanmycin and oryzanol, and the mass ratio of ningnanmycin to oryzanol is 1: (0.1-5). According to the anti-virus composition containing the oryzanol and the ningnanmycin and the application of the anti-virus composition, the oryzanol and the ningnanmycin have a remarkable synergistic effect, and the anti-virus composition is suitable for preventing and treating plant virus diseases, fungal diseases and bacterial diseases and has the effects of regulating plant growth and enhancing stress resistance.