Synthesis method of O-quaternized vanilla-based carbon residue particles based on amphiphilic biological carbon material

A technology of bio-carbon material and synthesis method, which is applied in the field of synthesis of O-quaternized vanillyl chitosan carbon slag particles, can solve the problems of few biocompatible antibacterial materials, and achieve excellent broad-spectrum antibacterial properties, The effect of expanding the application range and uniform and stable dispersion

CN113444254APending Publication Date: 2021-09-28浙江科曼奇生物科技股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-09-28

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Abstract

The invention discloses a synthesis method of O-quaternized vanilla-based chitosan carbon residue particles based on an amphiphilic biological carbon material, and belongs to the technical field of biology. The synthesis method comprises the following steps: preparing an amphiphilic biological carbon material from beer yeast cells by adopting a hydrothermal synthesis method, grafting chitosan by adopting a covalent binding method by taking the amphiphilic biological carbon material as a carrier, and carrying out structure modification on chitosan carbon residue particles by adopting a chemical method by taking vanillin and glycidyl trimethyl ammonium chloride as modified materials, and obtianing the functionalized carbon residue particles. A series of characterization means verify successful covalent modification of chitosan, vanillin and glycidyl trimethyl ammonium chloride. The functionalized carbon residue particles synthesized by the invention still have high dispersity in polar and non-polar solvents while retaining the advantage of antibacterial property of quaternized chitosan. The synthesis method is simple in process and low in cost. The synthetic product is low in irritation, good in biocompatibility, excellent in antibacterial property and good in amphipathy.
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Description

technical field

[0001] The invention relates to the fields of micron material science and biotechnology, and in particular to a method for synthesizing O-quaternized vanilla-based chitosan carbon slag particles based on amphiphilic bio-carbon materials. Background technique

[0002] The skin is one of the largest and most important organs of the human body. Since the skin is exposed to the external environment for a long time, it may be attacked by microorganisms at any time. Microorganisms have always been one of the important factors that destroy biological health. At present, there are not many antibacterial materials with good antibacterial performance, good biocompatibility and non-irritating on the market.

[0003] Chitosan is the only alkaline polysaccharide that exists in nature. It has good film-forming properties, good protein affinity, no skin allergies, no irritation and no toxic reactions. At the same time, it can also enhance the skin's immunity to infections ...

Examples

Embodiment 1

[0038] refer to Figure 1-4 As shown, the synthesis of O-quaternized vanillyl chitosan carbon residue particles based on amphiphilic biochar materials:

[0039](1) Preparation of amphiphilic bio-carbon material: Weigh 10 g of beer yeast cells (provided by Henan Aoke Brewery), add 80-100 ml of deionized water and stir to disperse evenly. In this embodiment, the amount of deionization added can be selected 90ml, 87ml or 96ml, the dispersion liquid is centrifuged at 8000r / min for 2min, the supernatant is discarded, the precipitate is added with an appropriate amount of acetone, the glass is stirred to mix, and placed on a shaker at 180r / min for 15-20min. In the embodiment, the glass cup is placed on a shaker and oscillated at 180r / min for 16min, 18min or 19min, centrifuged, and the supernatant liquid is poured off to obtain the yeast cell wall, and the above operation is repeated 3 to 5 times in the same way. Choose to repeat 4 or 5 times. Centrifugal wash with deionized water ...