Preservation solution for animal tissue and cells at low temperature and its application method
A technology of animal tissue and solution, which is applied in the field of preservation of animal tissue and cells, can solve problems such as solutions that have not yet been seen, and achieve the effects of convenient use, improved success rate, and maintenance of viability and biological activity
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Embodiment 1
[0020] The preparation of the in vitro preservation solution of embodiment 1 animal tissue and cell
[0021] Prepare animal tissue and cell preservation solutions according to the component ratios in Table 1. Each experimental group is configured according to the components and ratios within the protection scope of the claims of the application, and each control group is a certain component missing or group The content in weight percentage is beyond the protection scope of the claims of the present application.
[0022] Table 1 The composition of the preservation solution of animal tissues and cells and the parameters of ultrasonic irradiation treatment
[0023]
[0024]
[0025] Note: " / " means that the item does not exist, and "*" means that the index is beyond the protection scope of the claims of this application.
Embodiment 2
[0026] Example 2 Activity evaluation after treatment with mouse hepatocyte preservation solution
[0027] In this example, the animal tissue and cell preservation solution prepared in Example 1 was used to evaluate the preservation effect of primary mouse hepatocytes.
[0028] Using the two-step collagenase perfusion method, the primary mouse hepatocytes were isolated and equally divided into several parts, which were respectively placed in the preservation solutions of different experimental groups and control groups in Table 1. Cultivate in different incubators at ℃ and 37℃ for 6 days, replace the storage solution every 24 hours, and immediately perform ultrasonic irradiation treatment after changing the solution (see Table 1 for operating parameters), and use trypan blue staining method to evaluate mouse liver cells after 6 days activity, the results are shown in Table 2.
[0029] From the results in Table 2, it can be seen that the activity of mouse hepatocytes in each ex...
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