The invention relates to a traditional Chinese medicine composition for invigorating the kidney, strengthening yang, replenishing essence, and reinforcing vitality, and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following thirty five traditional Chinese medicines: cynomorium songaricum, rhizoma curculiginis, morinda root, Angelica sinensis, fructus cnidii, broomrape, Chinese chive seed, Chinese magnoliavine fruit, red ginseng, bull penis, dog kidney, antler, black sliced aconite, cinnamon, common fennel, actinolite, pepper, dodder, eucommia, flasem milkvetch seed, Codonopsis pilosula, cornel, herba epimedii, Astragalus root, Chinese yam, prepared rehmannia root,fructus psoraleae, wolfberry fruit, raspberry, polygala, lotus stamen, cherokee rose, rhizoma alismatis,licorice and tuckahoe. The traditional Chinese medicine composition has the efficacies of invigorating the kidney, strengthening yang, replenishing essence, and reinforcing vitality, is mainly used for the diseases of insufficiency of impotence, spermatorrhea, prospermia and the like caused by the kidney-yang or deficiency of the kidney-yin, has remarkable clinical pharmacodynamic testing effect, is high in bioavailability, and has not any toxic or side effect.