Preparation method and applications of pig spleen transferfactor

A technology of pig spleen transfer factor and spleen, which is applied to the preparation methods of peptides, chemical instruments and methods, pharmaceutical formulations, etc., can solve the problems of low titer of transfer factors and high temperature, and achieves reducing the risk of disease occurrence, improving production performance, shortening The effect of the body's immune response

Inactive Publication Date: 2016-03-02
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention aims at the technical defects of the prior art, and provides a preparation method and application of porcine spleen transfer factor, so as to solve the problem that the titer of the transfer factor prepared by the prior art method is low
[0011] Another technical problem to be solved by the present invention is that the transfer factor titer prepared by the prior art method is relatively low due to the high temperature of the operation process.

Method used

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  • Preparation method and applications of pig spleen transferfactor
  • Preparation method and applications of pig spleen transferfactor
  • Preparation method and applications of pig spleen transferfactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 (preparation of transfer factor injection)

[0038] (1) Receiving materials According to the production plan, receive 50kg of frozen raw materials and enter the production area to remove the outer packaging;

[0039] (2) Coarse grinding According to the operating procedures of the frozen meat grinder, the frozen meat grinder is coarsely ground, using a 3mm sieve, adding 0.5 times the 8°C injection water to the discharge and mixing, and stirring into a fluid state;

[0040] (3) Fine wringing According to the operating procedures of the colloid mill, the colloid mill grinds the rough wringing liquid 2 to 3 times, adds 1 times 8°C injection water, mixes well, and harvests 123 kg of fine wringing liquid;

[0041] (4) The frozen and thawed semen liquid was repeatedly frozen and thawed 6 times at -20°C → 37°C;

[0042] (5) Centrifuge the frozen-thawed feed solution at 10°C and centrifuge at 10000r / min to remove the precipitate and harvest the supernatant, a tota...

Embodiment 2

[0048] Embodiment 2 (preparation of transfer factor injection)

[0049] (1) Receiving materials According to the production plan, receive 60kg of frozen raw materials and enter the production area to remove the outer packaging;

[0050] (2) Coarse grinding According to the operating procedures of the frozen meat grinder, the frozen meat grinder is coarsely ground, using a 5mm sieve, adding 0.8 times 10 ℃ injection water to the discharge and mixing, stirring into a fluid state;

[0051] (3) Fine wringing According to the operating procedures of the colloid mill, the colloid mill grinds the rough wringing liquid 2 to 3 times, adds 0.7 times of 10°C injection water, mixes well, and harvests 147 kg of fine wringing liquid;

[0052] (4) The frozen and thawed semen liquid was repeatedly frozen and thawed 8 times at -20°C → 37°C;

[0053] (5) Centrifuge the frozen-thawed feed solution at 15°C, centrifuge at 8000r / min, remove the precipitate, and harvest the supernatant, a total of 1...

Embodiment 3

[0059] Embodiment 3 (preparation of transfer factor injection)

[0060] (1) Receiving materials According to the production plan, receive 50kg of frozen raw materials and enter the production area to remove the outer packaging;

[0061] (2) Coarsely grind according to the operating procedures of the frozen meat grinder, use a 5mm sieve for rough grinding, add 1 times 7 ℃ injection water to the discharge and mix, and stir into a fluid state;

[0062] (3) Fine wringing According to the operating procedures of the colloid mill, the colloid mill grinds the rough wringing liquid 2 to 3 times, adds 0.7 times of 7°C injection water, mixes well, and harvests 124 kg of fine wringing liquid;

[0063] (4) The frozen and thawed semen liquid was repeatedly frozen and thawed 7 times at -20°C → 37°C;

[0064] (5) Centrifuge the frozen-thawed feed solution at 10°C, centrifuge at 9000r / min to remove the precipitate, and harvest the supernatant, a total of 86.5kg;

[0065] (6) Ultrafiltration...

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Abstract

The invention provides a preparation method and applications of pig spleen transferfactor. According to the preparation method, frozen-breaking, freeze thawing breaking, and centrifugal process are combined for transferfactor production, yield is guaranteed, and at the same time production temperature is reduced, and the activity of the transferfactor can be guaranteed preferably. And in addition, the prepared transferfactor contains no protein, is a small molecular peptide, possesses no pyrogen, is a widely used immunomodulator, is capable of increasing vaccine titer, and can be used for improving animal immunity and improving production performance; the activity is not influenced by pancreatic enzymes; and no toxic reaction is caused. When the pig spleen transferfactor is combined with vaccine, animal immunity can be improved, vaccine titer is increased at the same time, body immune response period is shortened, and antibody lasting time is prolonged.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a preparation method and application of pig spleen transfer factor. Background technique [0002] Transfer factor (TF for short) is a substance released by lymphocytes that can transfer sensitization information. It does not contain protein and is a small molecular polypeptide. It has the functions of transmitting immune information, regulating immune function, and enhancing immune function of the body. TF has no pyrogen and is not destroyed by trypsin. The activity of TF is unstable to heat and stable under freezing conditions. It can specifically transfer the donor's cellular immune information to the recipient, thereby enhancing the immune function of the recipient. At present, it has become a widely used immune-enhancing preparation in foreign countries. [0003] 1. Preparation process [0004] Currently, the prior art for preparing transfer factor ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34A61K38/02A61K35/26A61P37/04
Inventor 王尚尚苏建东杨保收付旭彬梁武
Owner TIANJIN RINGPU BIO TECH
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