The invention discloses a preparation method of a novel sea-buckthorn feed for laying hens, and discloses a sea-buckthorn feed and a production method thereof. The sea-buckthorn feed comprises corn flour, soybean meal, edible salt, rapeseed oil, folium artemisiae argyi, DL-methionine, L-lysine hydrochloride, calcium hydrogen phosphate, shell powder, wheat bran, sea-buckthorn fruits and a premix. By adopting the preparation method of the novel sea-buckthorn feed for laying hens, the problems that a traditional feed is lack of biotin, incomplete in nutrition or unstable in biotin content and lowin utilization rate, and the normal growth and development of laying hens are influenced are solved.