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Selenium-rich tea polysaccharide with blood sugar reducing effect and preparation method of lozenge of selenium-rich tea polysaccharide

A selenium-enriched tea and hypoglycemic technology, which is applied to medical preparations containing active ingredients, sulfur/selenium/tellurium active ingredients, pharmaceutical formulations, etc. and other problems, to achieve the effect of strengthening the hypoglycemic effect, being convenient to carry and taking, and satisfying natural health.

Pending Publication Date: 2022-05-06
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because selenium polysaccharides are easy to absorb moisture and oxidize, they are not easy to store, carry and take, so they cannot be directly applied to the market.
At present, products such as compound selenium polysaccharide capsules and lentinan capsules have been launched, but the development of tea selenium polysaccharide terminal products has not yet been reported.

Method used

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  • Selenium-rich tea polysaccharide with blood sugar reducing effect and preparation method of lozenge of selenium-rich tea polysaccharide
  • Selenium-rich tea polysaccharide with blood sugar reducing effect and preparation method of lozenge of selenium-rich tea polysaccharide
  • Selenium-rich tea polysaccharide with blood sugar reducing effect and preparation method of lozenge of selenium-rich tea polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for preparing natural selenium-enriched tea polysaccharide with hypoglycemic effect, the following steps are adopted:

[0042] 1) Crush 1.20kg of selenium-enriched tea leaves, add 70°C hot water at a material-to-liquid mass ratio of 1:30, extract with 45kHz ultrasound for 30min, and repeat the extraction twice;

[0043] 2) The extract was centrifuged, and the supernatant was concentrated by vacuum rotary evaporation at -0.10MPa vacuum, 80r / min speed, and 65°C water bath temperature to 1 / 6 of the original volume; after the concentrate was cooled, ethanol was added until its concentration reached 75% ( v / v), centrifugal;

[0044] 3) The precipitate was dissolved, dialyzed with 2kDa cut-off pore size semi-permeable membrane water for 12 hours, and freeze-dried to obtain natural selenium-enriched tea polysaccharide.

[0045] A method for preparing selenium-enriched tea polysaccharide buccal tablets with hypoglycemic effect, the following steps are adopted:

[004...

Embodiment 2

[0050] A method for preparing natural selenium-enriched tea polysaccharide with hypoglycemic effect, the following steps are adopted:

[0051] 1) Crush 1.00kg of selenium-enriched camellia, add hot water at 65°C according to the mass ratio of solid to liquid at 1:40, extract ultrasonically at a frequency of 55kHz for 30min, and repeat the extraction twice;

[0052] 2) The extract was centrifuged, and the supernatant was concentrated by vacuum rotation at -0.09MPa vacuum, 60r / min speed, and 60°C water bath temperature to 1 / 5 of the original volume; after the concentrate was cooled, ethanol was added until the concentration reached 70% ( v / v), centrifugal;

[0053]3) The precipitate was dissolved, dialyzed with a semipermeable membrane with a cut-off pore size of 2kDa for 12 hours, and freeze-dried to obtain natural selenium-enriched tea polysaccharide.

[0054] A method for preparing selenium-enriched tea polysaccharide buccal tablets with hypoglycemic effect, the following st...

Embodiment 3

[0059] A method for preparing artificial selenium-enriched tea polysaccharide with hypoglycemic effect, the following steps are adopted:

[0060] 1) Dissolve 2.0g of ordinary tea polysaccharide in 300mL of glacial acetic acid, add 1.80g of potassium selenite and 0.10g of barium bromide, and stir for 7 hours in a water bath at 80°C;

[0061] 2) adding potassium hydroxide to adjust the pH of the solution to 7.5, and dialysis with 3.5kDa pore size semi-permeable membrane water until the retentate is detected by sodium sulfate without precipitation and detected by Vc as colorless;

[0062] 3) freeze-drying to obtain artificial selenium-enriched tea polysaccharide.

[0063] A method for preparing selenium-enriched tea polysaccharide buccal tablets with hypoglycemic effect, the following steps are adopted:

[0064] a) Superfinely pulverize selenium-enriched tea leaves, mint leaves, dried lemons, and stevia leaves respectively, and pass through a 100-mesh sieve;

[0065] b) Take 7 ...

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Abstract

The invention discloses selenium-rich tea polysaccharide with a blood sugar reducing effect and a preparation method of a lozenge of the selenium-rich tea polysaccharide, and belongs to the technical field of deep processing of tea leaves. The selenium-enriched tea polysaccharide lozenge is prepared by tabletting the following raw materials in parts by weight: 5-20 parts of selenium-enriched tea polysaccharide, 12-20 parts of selenium-enriched tea leaves, 7-12 parts of mint leaves, 3-5 parts of dried lemon, 3-8 parts of stevia rebaudian, 45-65 parts of a filler and 0.5-5 parts of a lubricant. The selenium-rich tea polysaccharide is natural selenium-rich tea polysaccharide extracted from selenium-rich tea or artificial selenium-rich tea polysaccharide synthesized by tea polysaccharide and inorganic selenium. The research discovers that both the natural selenium-enriched tea polysaccharide and the artificial selenium-enriched tea polysaccharide have good in-vitro blood sugar reducing activity and can effectively inhibit the activity of alpha-glucosidase, and the inhibitory activity of the natural selenium-enriched tea polysaccharide and the artificial selenium-enriched tea polysaccharide is remarkably higher than that of common tea polysaccharide. The lozenge prepared by taking the selenium-rich tea polysaccharide as an active ingredient is safe and efficient, can well supplement selenium necessary for a human body, has the effect of reducing blood sugar, and has a good application prospect in hyperglycemia / diabetes patient groups.

Description

technical field [0001] The invention relates to the technical field of deep processing of tea, in particular to a selenium-enriched tea polysaccharide with the effect of lowering blood sugar and a preparation method thereof. Background technique [0002] Diabetes mellitus is a common metabolic disease in humans, clinically characterized by chronic fasting hyperglycemia. Long-term hyperglycemia can cause various macrovascular and microvascular complications, such as coronary artery disease, peripheral artery disease, diabetic nephropathy, and retinopathy. According to statistics from the International Diabetes Federation, about 463 million adults worldwide suffer from diabetes in 2019, and it is estimated that by 2030, the number of diabetic patients will reach 578.4 million. Diabetes has become one of the most serious public health problems worldwide. [0003] Tea is the second most consumed beverage after water and is widely consumed by people all over the world. In Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/82A61K31/715A61K33/04A61K9/20A61P3/10
CPCC08B37/0003C08B37/006A61K31/715A61K33/04A61K36/82A61K36/534A61K36/752A61K36/287A61K9/2013A61K9/2009A61K9/0056A61P3/10A61K2300/00C08L5/00A61K36/28
Inventor 魏新林程利增王元凤魏康彭斓兰
Owner SHANGHAI JIAO TONG UNIV
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