Selenium-rich tea polysaccharide with blood sugar reducing effect and preparation method of lozenge of selenium-rich tea polysaccharide
A selenium-enriched tea and hypoglycemic technology, which is applied to medical preparations containing active ingredients, sulfur/selenium/tellurium active ingredients, pharmaceutical formulations, etc. and other problems, to achieve the effect of strengthening the hypoglycemic effect, being convenient to carry and taking, and satisfying natural health.
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Embodiment 1
[0041] A method for preparing natural selenium-enriched tea polysaccharide with hypoglycemic effect, the following steps are adopted:
[0042] 1) Crush 1.20kg of selenium-enriched tea leaves, add 70°C hot water at a material-to-liquid mass ratio of 1:30, extract with 45kHz ultrasound for 30min, and repeat the extraction twice;
[0043] 2) The extract was centrifuged, and the supernatant was concentrated by vacuum rotary evaporation at -0.10MPa vacuum, 80r / min speed, and 65°C water bath temperature to 1 / 6 of the original volume; after the concentrate was cooled, ethanol was added until its concentration reached 75% ( v / v), centrifugal;
[0044] 3) The precipitate was dissolved, dialyzed with 2kDa cut-off pore size semi-permeable membrane water for 12 hours, and freeze-dried to obtain natural selenium-enriched tea polysaccharide.
[0045] A method for preparing selenium-enriched tea polysaccharide buccal tablets with hypoglycemic effect, the following steps are adopted:
[004...
Embodiment 2
[0050] A method for preparing natural selenium-enriched tea polysaccharide with hypoglycemic effect, the following steps are adopted:
[0051] 1) Crush 1.00kg of selenium-enriched camellia, add hot water at 65°C according to the mass ratio of solid to liquid at 1:40, extract ultrasonically at a frequency of 55kHz for 30min, and repeat the extraction twice;
[0052] 2) The extract was centrifuged, and the supernatant was concentrated by vacuum rotation at -0.09MPa vacuum, 60r / min speed, and 60°C water bath temperature to 1 / 5 of the original volume; after the concentrate was cooled, ethanol was added until the concentration reached 70% ( v / v), centrifugal;
[0053]3) The precipitate was dissolved, dialyzed with a semipermeable membrane with a cut-off pore size of 2kDa for 12 hours, and freeze-dried to obtain natural selenium-enriched tea polysaccharide.
[0054] A method for preparing selenium-enriched tea polysaccharide buccal tablets with hypoglycemic effect, the following st...
Embodiment 3
[0059] A method for preparing artificial selenium-enriched tea polysaccharide with hypoglycemic effect, the following steps are adopted:
[0060] 1) Dissolve 2.0g of ordinary tea polysaccharide in 300mL of glacial acetic acid, add 1.80g of potassium selenite and 0.10g of barium bromide, and stir for 7 hours in a water bath at 80°C;
[0061] 2) adding potassium hydroxide to adjust the pH of the solution to 7.5, and dialysis with 3.5kDa pore size semi-permeable membrane water until the retentate is detected by sodium sulfate without precipitation and detected by Vc as colorless;
[0062] 3) freeze-drying to obtain artificial selenium-enriched tea polysaccharide.
[0063] A method for preparing selenium-enriched tea polysaccharide buccal tablets with hypoglycemic effect, the following steps are adopted:
[0064] a) Superfinely pulverize selenium-enriched tea leaves, mint leaves, dried lemons, and stevia leaves respectively, and pass through a 100-mesh sieve;
[0065] b) Take 7 ...
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