The invention pertains to the technical field of food detection and analysis, in particular to a multi-residue detection kit of sulfonamides in animal derived food and the detection method thereof, the kit is composed of a box body, a coated plate and a bracket, the coated plate and the bracket are fixedly arranged in the box body, wherein, (1) a concave hole of the standard solution of sulfonamides, (2) the concave hole of antigen working solution of enzyme labeled sulfonamides, (3) the concave hole of antigen dilute solution of enzyme labeled sulfonamides, (4) the concave hole of substrate chromogenic agent A solution, (5) the concave hole of substrate chromogenic agent B solution, (6) the concave hole of stop solution, and (7) the concave hole of concentrated washing liquid are arranged on the bracket. The invention is characterized by specificity, sensitiveness, accuracy, high speed, convenience, low cost and so on; furthermore, the scope of application is broad, the detection efficiency is high, the operation is simple, and the invention has both economic benefits and social benefits.