116results about How to "Good broad spectrum" patented technology

The invention discloses a preparing method of an oil field demulsifier. The preparing method adopts ethyleneamine as a raw material, and the oil field demulsifier is prepared by crosslinking modification of the ethyleneamine, ethylene oxide, and propylene epoxide. The method comprises the following steps of: with the ethyleneamine as an initiator, enabling the ethyleneamine to react with propylene oxide and ethylene oxide to prepare an ethyleneamine type polyether demulsifier under the existence of a basic catalyst; carrying out self-crosslinking reaction on the polyether demulsifier A by adopting polycarboxylic acid under the action of a catalyst to prepare a product B; and finally carrying out self-crosslinking reaction on the polyether demulsifier A and the product B in a solvent by adopting toluene diisocynate to prepare a high performance demulsifier as a final product. The demulsifier prepared by the preparing method has the advantages of higher relative molecular weight, favorable dehydration effect and favorable broad spectrum, and can be used for demulsification and dehydration of the crude oil in the oil field. The dehydrated water is clear, and the dosage of the demulsifier is less.

The invention relates to a multiple combination oil soluble demulsifier which comprises 36-50 percent of demulsifier, 5-10 percent of surfactant containing fluorin and 40-59 percent of solvent, wherein the demulsifier comprises modified polyether taking phenolic resin as an initiator and modified polyether taking higher aliphatic alcohol as an initiator, and the weight ratio of the modified polyether taking the phenolic resin as the initiator to the modified polyether taking the higher aliphatic alcohol as the initiator is 0.8-3. The surfactant containing fluorin is preferably sodium perfluorous nonenyloxybenzenesulfonyl, perfluorous ethyldiethanolamine sodium phosphate or N-ethyl Perfluorooctanesulfonamide. The invention is mainly applied to atmospheric and vacuum process of a refinery with the operation temperature of 70-150 DEG C. When the use level is 3-30 ppm, the demulsifier has the advantages of good demulsifying effect, clear water and emulsion layers of demulsifying, high dehydration speed, low water content in purified oil, low oil content of water of dehydration, good broad spectrum activity and low cost.

The invention relates to a method for preparing bactericide by using derivative bacterial of growth promoting antagonist bacterial M18, preparing fermentation liquor with high concentration of under phenazine-1-carboxyl acid and pyoluteorin under optimized culture medium and condition by using derivative bacterial M18G and M18R of growth promoting antagonist bacterial M18, preparing the fermentation liquor M18G and M18R into dry powder, double crossing physically the phenazine-1-carboxyl acid in M18G powder and pyoluteorin in M18R according to the weight ratio of 90%-10% and 10%-90%, and finally getting bactericide of high efficiency for preventing plant disease. Compared with current technology, the bactericide provided in this invention is wet powder taking metabolite of microorgsanism as active element but not live bacterial agent, as a result of which the product is characterized by high stability, not easy to be influenced by environment and better prevention and curing effect for multiple plant disease under low consumption.

A broad-spectrum deemulsifier for crude oil contains the polymer of polyol, ethylene oxide and propylene oxide (20-50 wt.%), the polymen of polyamine, ethylene oxide and propylene oxide (20-50), pour depressor (10-20) and solvent (15-25). Its advantages are broad spectrum for more than 10 kinds of crude oils, and low dosage (5-10 ppm).

The invention relates to a neutralizing antibody of coronavirus or an antigen-binding fragment thereof, a nucleic acid molecule encoding the antibody or the antigen-binding fragment thereof, a vectorcontaining the nucleic acid molecule, a host cell containing the vector, application of the antibody or the antigen-binding fragment thereof in preparation of drugs for treating or preventing diseasescaused by coronavirus, and application in detection of products. The coronavirus antibody and the antigen-binding fragment thereof have broad-spectrum and strong neutralizing ability for various coronaviruses including SARS-CoV-2, and have good clinical application prospects in the future.

The invention discloses a botanical fungiticide prepared by carrying out combined extraction on Chinese herbal medicines. The botanical fungiticide comprises the active ingredients prepared by carrying out combined extraction on the following Chinese herbal medicines in parts by weight: 20-30 parts of sophora flavescens, 20-50 parts of dandelion, 50-80 parts of radix euphorbiae lantu, 20-40 parts of seriphidium finitum and 10-30 parts of Chinese gall; a preparation method of the botanical fungiticide comprises the steps of drying all the Chinese herbal medicines, and then grinding; soaking the ground Chinese herbal medicines into ethyl alcohol for 2-8h; carrying out cycle extraction for 2-8 times, wherein the extraction is carried out for 40-60min each time; removing the residue of the medicines, putting the extracting solution into a rotary evaporator and carrying out vacuum concentration; after the solution is concentrated to the fixed volume, feeding pesticide adjuvant with the fixed volume, and evenly stirring to obtain the botanical fungiticide. The botanical fungiticide has the characteristics of being stable in preparation, remarkable in sterilizing effect, good in broad spectrum property and safe for people and livestock.

The invention discloses a preparation method of a high-performance complex oilfield demulsifier, which comprises the following steps of: firstly preparing polyether demulsifiers of different molecular weights, i.e., a polyether demulsifier A of molecular weight 300-500, a polyether demulsifier B of molecular weight 700-900, a polyether demulsifier C of molecular weight 1500-2000, a polyether demulsifier D of molecular weight 4000-5000 and a polyether demulsifier E of molecular weight 8000-10000; and the polyether demulsifiers of various different molecular weights are compounded in certain proportion to obtain the high-performance demulsifier. The high-performance demulsifier prepared by the invention can be suitable for demulsification and dehydration of crude oil from an oilfield, and has the advantages of good broad spectrum and less consumption.

The present invention discloses a primer set for detecting mycoplasma. The primer set comprises a forward primer having a sequence represented by SEQ ID No.1; and a reverse primer having a sequence represented by SEQ ID NO.2. The invention further discloses a kit for detecting the mycoplasma by using the primer set, uses of the kit in detection of mycoplasma pollution, and a detection method for detecting the mycoplasma pollution by using the kit. The kit of the present invention has advantages of simple operation, broad spectrum, strong specificity and high sensitivity, can rapidly detect the mycoplasma pollution, and has wide application prospect.

A degrading bacteria BYS-1 for degrading phenylacetic acid under hyperhaline condition is disclosed. It is halomonas sp, belongs to moderate halophil. Its characteristics are G-, nemaline, (0.8-1.0)X(1.5-2.5)mum, flagellate, opal, semi-transparent. Contact enzyme and oxidase are positive, and it can hydrolyze starch and ferment glucose, reduce NO3- and growth under condition of 0-3.4M NaCl. It can be used in waste water biochemical treating system by phenylacetic acid.

The invention discloses a pesticide in particular for the prevention and cure of rice borer, including Chilo Suppressaeis Walker, Tryporyza incertulas, Sesamia inferens, Cnaphalocrocis medinalis Guenee and cotton bollworm, Red Spiders, Linnaeus, its active ingredients include Indoxacarb and acetamiprid, it has stable medicinal effect and and good wide spectrum property.

The invention discloses a medium trace element fertilizer which comprises the following components: 0.1-0.5 part of alginate, 30-40 parts of calcium ammonium nitrate, 10-15 parts of magnesium nitrate, 7-20 parts of sulfate, 5-10 parts of sodium borate and 25-50 parts of water. The preparation method comprises the following steps: according to the ratio of the raw materials, sequentially adding water, magnesium nitrate, sulfate and borate into a reaction kettle, adjusting the pH value, and controlling the temperature to be 50-60 DEG C and the rotation speed to be 20-25r / minute; further adding calcium ammonium nitrate and sodium alginate, controlling the temperature to be 60-70 DEG C and the rotation speed to be 10-15r / minute; after a gel is formed, increasing the rotation speed to be 40-50r / minute, and drying. The alginate fused with a biological source is beneficial for a saturated fertilizer solution to form the gel, medium trace elements can be prevented from being fixed by soil, the effect of slow and controlled release of nutrients can be achieved, the moisture utilization rate can be increased, and the medium trace element fertilizer can be also used as a liquid fertilizer additive. The invention further provides application of the medium trace element fertilizer.

The invention discloses a multiplex PCR detection method and kit for Shiga toxin-producing Escherichia coli and application thereof. The method and the kit thereof can simultaneously detect the genes consisting of stx1, stx2 and wzyO157 through a triplex PCR reaction, electrophoresis staining and ultraviolet visualization, subtypes consisting of stx1 and stx1c can be amplified by using a detection primer for the stx1 gene, subtypes consisting of stx2, stx2c and stx2d can be amplified by using a detection primer for the stx2 gene, and an O157 serotype strain can be identified by using a detection primer for the wzyO157 gene. According to the invention, the method and the kit can effectively detect stx and main variants thereof and can be used for distinguishing O157 from other non-O157 Escherichia coli, for directly detecting Shiga toxin-producing Escherichia coli in a food sample and for identifying a clinical strain and a food isolate.

The invention discloses a magnetic material loaded N-heterocyclic carbene copper catalyst as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis. The preparation method comprises: by taking 4-chloromethyl phenethyl trimethoxysilane as a raw material, carrying out benzyl chloride functionalization on the surfaces of SiO2 coated Fe3O4 magnetic nanoparticles to prepare an intermediate product 1; by taking N-substituted imidazole as a raw material, carrying out imidazolium salt grafting on the surface of the intermediate product 1 to prepare an intermediate product 2; dispersing cuprous halide, sodium tert-butoxide and the intermediate product 2 into a solvent, and carrying out stirring reaction at room temperature to obtain the magnetic material loaded N-heterocyclic carbene copper catalyst. The catalyst is applied for catalyzing alkyne and organic azide addition reaction to prepare 1,2,3-triazole compounds, and catalyzing a coupling reaction of terminal alkyne, aldehyde and amine to prepare a propargylamine compound. The catalyst can be repeatedly used for multiple times without reducing the catalytic activity.

The invention discloses Paenibacillus peoriae and application thereof in prevention and treatment of bacterial soft rot of cucumbers. The invention provides Paenibacillus peoriae ZF390 with the preservation number of CGMCC No. 20322. The invention also protects a fermentation product of the Paenibacillus peoriae ZF390. The invention also protects a microbial agent containing the Paenibacillus peoriae ZF390. The invention also protects application of the Paenibacillus peoriae ZF390 or the fermentation product or the microbial agent in inhibition of pathogenic bacteria. The invention also protects application of the Paenibacillus peoriae ZF390 or the fermentation product or the microbial agent in prevention and treatment of plant diseases caused by the pathogenic bacteria. The Paenibacillus peoriae ZF390 provided by the invention can be used for inhibiting the plant pathogenic bacteria, so that the plant diseases caused by the plant pathogenic bacteria are prevented and controlled.

The invention pertains to the technical field of food detection and analysis, in particular to a multi-residue detection kit of sulfonamides in animal derived food and the detection method thereof, the kit is composed of a box body, a coated plate and a bracket, the coated plate and the bracket are fixedly arranged in the box body, wherein, (1) a concave hole of the standard solution of sulfonamides, (2) the concave hole of antigen working solution of enzyme labeled sulfonamides, (3) the concave hole of antigen dilute solution of enzyme labeled sulfonamides, (4) the concave hole of substrate chromogenic agent A solution, (5) the concave hole of substrate chromogenic agent B solution, (6) the concave hole of stop solution, and (7) the concave hole of concentrated washing liquid are arranged on the bracket. The invention is characterized by specificity, sensitiveness, accuracy, high speed, convenience, low cost and so on; furthermore, the scope of application is broad, the detection efficiency is high, the operation is simple, and the invention has both economic benefits and social benefits.

The invention relates to a diamond bit and a manufacturing method thereof. The diamond bit comprises a work layer of an ordinary diamond bit and PCD sintered bodies which are distributed in the work layer, the PCD sintered bodies are columnar bodies, cuboids or cylinders and are distributed and arranged in a work layer matrix of the diamond bit, and the PCD sintered bodies account for 55-70 percent of the area of a work bottom lip of the diamond bit. The manufacturing method comprises the following steps: columnar PCD sintered bodies are precast by a hot press method; the diamond bit adopts secondary molding, the PCD sintered bodies are positioned in matrix alloy powder of the diamond bit in a graphite mould, and then the diamond bit is sintered by hot-pressing in a medium frequency electric furnace. The problem of difficult selection of the diamond bit aiming at rock stratum is solved. The diamond bit has the characteristics of good broad spectrum, good exposure, high efficiency and long service life. The diamond bit can be widely applied to geologic exploration, petroleum drilling, engineering investigation and geologic disaster control.

The invention relates to a reagent kit for quantificationally detecting the content of enrofloxacin in foods, belonging to the immunoassay technical field, consisting of a covering plate and a reagent group, wherein, the reagent group comprises buffer solution (1), a zymolyte A (2), a zymolyte B (3), scrub solution (4), stop solution (5), enrofloxacin standard solution (6) and enzyme-marked enrofloxacin antigen solution (7); a millipore of the covering plate is covered with polyclone enrofloxacin antibody. The invention has the characteristics of simple manipulation, high detection sensitivity, strong specificity, good accuracy and low cost of detection. Moreover, the invention is of wide range of application. With economic and social benefits, the invention is a reagent kit for quantificationally detecting the content of enrofloxacin in foods with high creativity and good potential applicaiton.

The invention provides a fusion protein of ribonuclease (Onc) and bacterial toxalbumin membrane transposition structural domain. The DNA sequence coding the fusion protein contains a vector of the DNA sequence, a host cell containing the vector and a method for preparing the fusion protein by adopting genetic engineering. The fusion protein not only has the activity of the original Onc, but also has stronger capability of killing tumor cells, and can be more widely applied to the anti-tumor treatment.

The invention discloses a foot-and-mouth disease virus non-structural protein 3B epitope peptide and an application thereof. A hybridoma cell line 2H1 is obtained through screening using a cell fusiontechnology, and a monoclonal antibody secreted by the hybridoma cell line 2H1 has a broad spectrum and a high affinity, and is highly competitive with serum. The antigen epitope recognized by the monoclonal antibody 2H1 is further identified accurately, and the amino acid sequence of the monoclonal antibody 2H1 is represented by SEQ ID No.2; and the antigen epitope peptide is mutated to obtain aseries of mutants. The antigen epitope peptide and the mutants thereof have good reaction ability with FMDV antibody positive serums of foot-and-mouth disease infected pigs, cattle and sheep, and an antibody ELISA detection kit established by using the antigen epitope peptide as envelope antigens can specifically distinguish FMDV infected serum and antigen immunized serum, can be adopted to detectthe serum of any animal species, spans the inter-species barrier, and has the advantages of high reliability, wide application range, good broad spectrum, strong competitiveness, and quickness and convenience in detection.

The invention discloses a method of improving the control effects of the lepidoptera pest of a medicine, belonging to the pest control technical field. The method is as follows: (1) the indoxacarb and the chlopyrifos are mixed according to the ratio 1:7-35 of the percentages by weight of the active ingredient of indoxacarb and the percentages by weight of the active ingredient of chlopyrifos; (2) with 8 to 40 grams of active ingredients for each mu is added with 30 to 60 liters of water to make the water solution; (3) during the crop lepidopterous larvae prime, the water solution is sprayed on the crop. The compound insecticide is capable of an oblivious synergistic action, the cotoxicity coefficient is more than 300, the drug resistance of pest is delayed, the pesticide use cost for each mu is lowered, the cost is reduced from 12 to 15 yuan for the indoxacarb single agent to 5 to 7 yuan for the mixing agent, the prevention and cure spectrum is enlarged and the high efficiency pesticide application time is prolonged. The method can be applied to control the lepidoptera pest and other pests of such crop as rice and vegetables etc.

The invention provides a polyphenol coating for capturing circulating tumor cells. A polyphenol type compound is modified on the surface of a material by adopting a surface modification method; the polyphenol coating can be used for rapidly and effectively enriching the circulating tumor cells. A capturing technology of the circulating tumor cells, provided by the invention, does not need to immobilize a specific cancer cell identification antibody or an aptamer, and specific capturing of different cancer cell types can be realized; a micro-nano base material does not need to be introduced, sothat the polyphenol coating has the advantages of low cost and convenience for operation. The prepared polyphenol coating can be used for capturing the circulating tumor cells in peripheral blood andis expected to be used for early diagnosis of patients with cancers.

The invention provides an avian influenza virus hemagglutinin antigen, a CHO cell strain expressing the same, a preparation method, and a vaccine, and relates to the field of biotechnology. The avianinfluenza virus hemagglutinin antigen is a hemagglutinin protein expressed by a sequence shown in SEQ ID NO. 1 and has the advantage of broad spectrum as an antigen, and the hemagglutinin protein is aprotein expressed by a eukaryotic cell, so that a conformational epitope which loses the antigen is avoided. The preparation method of the avian influenza virus hemagglutinin antigen and the cell strain expressing the avian influenza virus hemagglutinin antigen can obtain a hemagglutinin protein having broad spectrum and antigen immunogenicity. The vaccine of the avian influenza virus hemagglutinin antigen has low production cost and high immunological efficiency. After immunizing SPF chickens which are 3 to 4 weeks old, an H5 subtype antigen can be detected on the 14th day to determine the geometric mean titer (GMT) of an HI antibody (GMT) to be not less than 1:64, which is superior to conventional vaccines.

The invention discloses a hybridoma cell line secreting a foot-and-mouth disease virus non-structural protein monoclonal antibody 3A10, and an application thereof. The hybridoma cell line stably secreting the foot-and-mouth disease virus non-structural protein monoclonal antibody, with the microbial preservation number of CGMCC NO.16213, is finally obtained through screening using a cell fusion technology by immunizing mice with the foot-and-mouth disease non-structural protein. The monoclonal antibody secreted by the hybridoma cell line has the advantages of good broad spectrum, high conservatism, strong affinity and strong competitiveness with serum, and can be used as a detection antibody in a blocking ELISA detection method to accurately distinguish naturally infected animals with thefoot-and-mouth disease and immunized animals. The invention further provides a blocking ELISA detection kit established by using the monoclonal antibody as a detection antibody. The detection kit canbe adopted to detect the serum of any animal species, spans the inter-species barrier, and has the advantages of high reliability, wide application range, good broad spectrum, strong competitiveness,and quickness and convenience in detection.

The invention relates to a method for preparing biocontrol strain fermentation broth and particularly relates to a method for preparing broad-spectrum growth-promoting biocontrol bacillus B96-II fermentation broth which can prevent and treat blight-based soil-borne diseases of vegetables and melons and has a growth promoting effect on a variety of vegetables. The method comprises the following steps of: activating a strain; preparing a B96-II strain liquid culture medium; preparing B96-II strain liquid; preparing a B96-II fermentation broth culture medium; and preparing the B96-II fermentation broth. The bacillus B96-II fermentation broth prepared by the method has good broad-spectrum performance, can prevent and treat the diseases of a variety of vegetables and melons, and has a growth promoting effect on a variety of vegetables, so that the bacillus B96-II fermentation broth is widely applicable to the prevention and treatment of the soil-borne diseases of the vegetables and melons.

The invention provides an avian influenza virus hemagglutinin antigen, a preparation method and application thereof and an avian influenza vaccine and relates to the technical field of biology. The avian influenza virus hemagglutinin antigen is hemagglutinin protein expressed by a sequence shown as SEQ ID NO. 1 and is also a protein expressed by a mammalian expression system; the avian influenza virus hemagglutinin antigen has wide spectrum and good immunogenicity; after fowls are immunized with the immunogenicity, an antibody of high titer can be produced, and various subtype avian influenzaviruses can be prevented; the preparation method of the avian influenza virus hemagglutinin antigen is suitable for acquiring hemagglutinin protein having wide spectrum and good antigen immunogenicity; the preparation method is simple to perform and is suitable for large-scale production; a vaccine containing the avian influenza virus hemagglutinin antigen has low production cost and good immunization efficiency; after SPF (specific pathogen free) chicken which is 3-4 weeks old is immunized with the vaccine, it can be detected 14 days later that HI antibody GMT (geometric mean titer) measuredby H5 subtype antigen measurement is not less than 1:64 and is higher than that from the sue of conventional vaccines.

The invention provides a paddy compound pesticide and relates to a technology for producing pesticides, in particular to the technical field of production of paddy pesticides. The active ingredients of the paddy compound pesticide are buprofezin and monosultap which account for 12.5 percent and 62.5 percent of the total weight of the pesticide respectively. The pesticide of the invention is the high-efficiency paddy pesticide, has the unique effect of controlling paddy stem borers and planthoppers and also has good effects of contact poisoning, stomach poisoning, uptake translocation and rapid and high knock-down ability; moreover, the pesticide has the advantages of high extensive adaptability, good rainwater washing resistance, long residual period, long appropriate period for spraying and quick-acting property as well as long-lasting effect.

The invention discloses a 1, 5-benzodiazepine compound containing thiazolyl and ester and an application of the compound, and a structural formula of the compound is represented by a formula (I). The compound has a broader spectrum and higher activity for inhibiting funguses ad bacteria, and in-vitro antibacterial activity test result shows that the compound has a more obvious activity inhabiting effect and a higher killing effect on two types of funguses including cryptococcus neoformans clinical strains and standard strains as well as candida albicans, has a very high activity inhabiting effect and a very high killing effect on two types of bacteria including positive bacteria, namely staphylococcus aureus, as well as negative bacteria, namely escherichia coli, and can be taken as a candidate molecule and a lead compound for an antibacterial agent; and the structural formula of the compound is represented as the instruction shown.

The invention discloses a method for applying photoactive sepiolite Si-OH to adsorption and photodegradation of water-soluble organic pollutants. The method comprises the following steps of adding sepiolite into wastewater containing organic pollutants at the normal temperature, performing stirring to enable organic pollutant molecules to reach adsorption and desorption balance in an inner cavityand on the surface of the sepiolite, performing solid-liquid separation in a centrifugal or filtering manner to obtain organic matter adsorbed sepiolite, performing drying, then performing light source irradiation for enabling the sepiolite to generate Si-OH breakage under the action of a light source to generate hydroxyl radicals, further reacting the hydroxyl radicals with oxygen and the like inair to convert oxygen negative ion radicals and other active oxygen species, and then degrading the organic pollutants adsorbed to the inner cavity and the surface of the sepiolite, so that the effect of mineralizing or partially mineralizing the organic pollutants is achieved. The sepiolite added into an aqueous solution is good in stability, easy to recycle and recyclable. The method has the advantages of low cost, high treatment efficiency at the normal temperature, simplicity in operation and good industrial application value.

The present invention discloses a Bacillus cereus strain and microbial bactericide producing process with the strain. The Bacillus cereus strain is named Bacillus cereus 98-I and has good broad-spectrum property, high bacteriostatic activity, plant growth and germination promoting effect and plant resistance inducing effect. The microbial bactericide producing process with the strain includes slant culture of strain, shaking table preparation of strain, the first stage strain preparation and cylinder fermentation with the relevant parameters for every steps being described. The microbial bactericide has wide application in preventing and controlling soil-borne diseases of vegetable, melon, flower and lawn.

The present invention discloses a high-performance compounding type oil field emulsion breaker preparation method, which comprises: preparing polyether emulsion breakers with different molecular weights to obtain a polyether emulsion breaker A with a molecular weight of 300-500, a polyether emulsion breaker B with a molecular weight of 700-900, a high molecular weight polyether emulsion breaker C with a molecular weight of 1500-2000, a high molecular weight polyether emulsion breaker D with a molecular weight of 4000-5000, and a high molecular weight polyether emulsion breaker E with a molecular weight of 8000-10000; and compounding various polyether emulsion breakers with different molecular weights according to a certain ratio so as to prepare the high-performance emulsion breaker. According to the present invention, the prepared high-performance emulsion breaker can be used for emulsion breaking dewatering of the oil field crude oil, and has advantages of good broad spectrum and less consumption.