The invention discloses a gibberellic acid-containing herbicide composition and a preparation method as well as application thereof. The gibberellic acid-containing herbicide composition is prepared from the following components in parts by weight: 0.01 to 99 parts of gibberellic acid and 1 to 90 parts of herbicide; the herbicide is one or a mixture of at least two of cyhalofop-butyl, penoxsulam, metamifop, bispyribac-sodium, quinclorac, anilofos, pretilachlor, butachlor, mefenacet, bensulfuron methyl, pyrazosulfuron-ethyl, pyriminobac-methyl, bentazone, mesotrione, halosulfuron-methyl, metolachlor, pendimethalin, oxadiazon, oxadiargyl, MCPa, fluroxypyr and diflufenican. The gibberellic acid-containing herbicide composition has synergism to weed prevention and control, the weed control efficiency is improved, the safety of the herbicide to crops can be improved, the herbicide phytotoxicity problem in agricultural production is fundamentally solved, and meanwhile the crop yield is improved by adjusting crop growth.