208results about How to "Has broad-spectrum antibacterial properties" patented technology

The invention discloses a nontoxic antifouling paint for an oceanographic ship and facilities and a method for preparing the paint. The paint consists of the following components in percentage by weight: 15 to 55 percent of base material, 0.01 to 10 percent of antifouling agent, 10 to 30 percent of filler, 0.3 to 25 percent of pigment, 2 to 8 percent of auxiliary agent and 3 to 55 percent of solvent, wherein the antifouling agent is silver grains with average grain diameter of between 1 and 200nm. The paint does not contain toxic agents, such as dichloro-diphenyl-trichloroethane, organic tin,cuprous oxide and the like, at all while effectively suppressing corrosion of marine biological attachments, meets the related regulations of international maritime organization and future development trend of the antifouling paint, realizes non-toxicity and environmental friendliness of the marine antifouling technology, and can be widely applied to the facilities and the fields, such as ships, floating piers, marine oil platforms, submarine cables, cooling pipes of power plant near the sea, sea water culture net cage and the like.

The present invention discloses one kind of nanometer chitosan quaternary ammonium salt / organic montmorillonite composite material and its preparation process. The composite material consists of chitosan quaternary ammonium salt and organic montmorillonite. It is prepared through twice dropping chitosan quaternary ammonium salt solution into organic montmorillonite suspension at 70-80 deg.c and through stirring, and freeze drying. The nanometer composite material combines the bacterium adsorbing and fixing effect of clay and the bacterium inhibiting and killing effect of chitosan quaternary ammonium salt, so that it has high antibiotic performance, broad antibiotic spectrum and long antibiotic period.

The invention belongs to the field of aseptic technologies integrating compound natural aseptic and bacteriostatic agents and flexible sterilizing conditions, and relates to a preparation method for an aseptic containing compound clove / cinnamon extracts and the aseptic application of the aseptic in sauce products. The compound clove / cinnamon extracts are firstly prepared, and the aseptic containing the compound clove / cinnamon extracts is prepared afterwards; the aseptic application of the aseptic in sauce products is characterized in that the addition of the aseptic with a mass fraction of 0.2 percent is integrated with the condition that sterilization is carried out for 20-30 minutes at a temperature of 85 DEG C for the aseptic and bacteriostatic purposes, so as to achieve the one-year shelf life of sauce products and good bacteriostatic and aseptic effects. According to the invention, the compound clove / cinnamon extracts have the advantages of high safety and wide bacteriostatic range, and the aseptic containing the compound clove / cinnamon extracts has high water solubility and stability; the aseptic method can not only kill microorganisms in sauce products but also better preserve nutrients in sauce products, and is an aseptic approach remarkably integrating natural aseptic materials and flexible sterilization, thereby achieving broad application prospects.

The invention provides an apple facial mask based on a traditional Chinese medicine formula.The apple mask comprises a silk matrix and a water-soluble substance.The water-soluble substance comprises apple extract, traditional Chinese medicine extract, seaweed extract, sodium hyaluronate, beta-glucan and assistant.The traditional Chinese medicine extract comprises tremella extract, centella asiatica extract, polygonum cuspidatum root extract, scutellaria root extract, tea extract, glycyrrhiza glabra root extract, chamomilla recutita flower extract, rosmarinus officinalis leaf extract and purslane herb extract.The seaweed extract contains chondrus crispus and bio-saccharide gum-1.The assistant comprises butanediol, propylene glycol, diglycerol, polyethylene glycol-400, Carbomer, hydroxyethyl cellulose, urea, allantoin, glycine betaine, nicotinamide, sodium glutamate, triethanolamine, 1,2-hexanediol, decoyl hydroximic acid, 1,3-propylene glycol, hexanediol and deionized water.The apple mask has the effects that stability is good, dispersibility is high, nutrients are proper in quantity and balanced, antibiosis and anticorrosion effects are achieved, and moisturizing, skin whitening and stain lightening are achieved.

A broad-spectrum antibacterial nano ointment is prepared from inorganic antibacterial silver nanoparticles, matrix, borneol, mint oil and menthol. Its advantages are high effect and safety, and no poison and irritation. It is suitable for skin wound, acne, beriberi, etc.

The invention discloses a formulation and a preparation method of an antibacterial spray with antibacterial, hemostasis and promotion of wound healing effects. The spray contains water which is used as a solvent, and chitosan, glycerol, polyhexamethylene guanidine and vitamin E which are used as main effective components. The antibacterial spray has obvious activity inhibition on growth of multiple types of pathogenic bacteria, the killing rate of the antibacterial spray on staphylococcus aureus, escherichia coli and candida albicans can reach 99%, and the antibacterial spray does not stimulate skins and mucosa, is suitable for treating skin and mucosa wound surface infection, and can be used for promoting the wound healing.

The invention discloses newly separated pediococcus acidilactici HEW-AP27 collected in the CGMCC (China General Microbiological Culture Collection Center) in the Institute of microbiology of CAS (Chinese Academy of Sciences) in Beichen west road No.1 yard No.3 in Chaoyang District of Beijing with the postal code of 100101 on March 7, 2018, with the collection number of CGMCC NO.15419. The invention also provides a preparation method of a viable pediococcus acidilactici preparation. The method comprises the steps as follows: the pediococcus acidilactici is used as a fermentation strain, an optimized fermentation process and a fermentation medium are adopted, discharging is performed after first-stage, second-stage and third-stage culture, active bacterial sludge is obtained through centrifugation, a stabilization protective agent is added, granulation is performed through a granulator, a coating liquid is added to a coating machine for coating, and finally, the viable pediococcus acidilactici preparation is obtained. The pediococcus acidilactici HEW-AP27 has acid resistance, cholate resistance and high-temperature resistance, has high acid producing performance and can inhibit growth and reproduction of pathogenic bacteria.

The invention discloses a photocatalytic antibacterial biomass wood-plastic composite material and a preparation method thereof. The photocatalytic antibacterial biomass wood-plastic composite material mainly comprises the following constituents in parts by weight: 25 to 75 parts of thermoplastic plastic, 15 to 75 parts of wood powder, 5 to 20 parts of an inorganic filler, 1 to 10 parts of a modified nano TiO2 carried Ag+ and Cu2+ complex antimicrobial agent, 1 to 5 parts of a fire retardant, 1 to 10 parts of a lubricating agent, and 1 to 8 parts of a coupling agent. The composite material takes the modified nano TiO2 carried Ag+ and Cu2+ complex antimicrobial agent, the (waste) thermoplastic plastic, the (waste) wood powder and other auxiliaries as raw materials, so that the harm of mildew and rot and fungal infection of the wood-plastic composite material and termite and other insects is avoided, the environment is protected, and the resource waste is reduced. Taking the modified nano TiO2 carried Ag+ and Cu2+ complex antimicrobial agent as powder, the composite material has the characteristics that antibacterial and bactericidal effect is rapid and efficient, the composite material is safe and nontoxic, does not produce drug-resistance bacteria, is thorough, durable and stable, and has broad-spectrum antibacterial property, and the like, and further has the characteristics of double long effectiveness of photocatalytic antibacterial and antifungal effect of TiO2 and antibacterial and bactericidal effect of Ag+ and Cu2+.

The invention relates to a natural plant essential oil microemulsion antibacterial spray and a preparation method thereof. The natural plant essential oil microemulsion antibacterial spray is composedof 10-40% of plant essential oil microemulsion and 60-90% of distilled water. According to the natural plant essential oil microemulsion antibacterial spray and the preparation method thereof of theinvention, essential oil extracted from natural plant materials is used as a main component, and the purpose of synergistic interaction is achieved by compounding different plant essential oils, so that the antibacterial effect is greatly enhanced, and the spray has a broad-spectrum antibacterial property; meanwhile, the functions of tranquilizing and allaying excitement, moisturizing and protecting skin, removing peculiar smell and providing fragrance are also realized.

The present invention relates to antibiotic dressing for treating injury of mammal, especially human body, and is especially a kind anti-coagulation nano silver antibiotic dressing prepared via anti-coagulation technology. The antibiotic dressing includes a kind of fabric and composite nano silver particles deposited onto the fabric, and the composite nano silver particles in the size of 1-100 nm are attached firmly between the fiber elements and on the fiber. The composite particles have core of metal silver and surface layer of silver oxide, which accounts for 10-80 wt% of the whole composite particles, and the silver content of the dressing is less than 50 microgram each squared millimeter.

The invention discloses a polymer surface protection monodisperse nano silver particle. A monodisperse ball-shaped efficient anti-agglomeration nano silver preparation is prepared in situ through a synthetic high polymer stabilizing additive containing sulfydryl under the effect of a mixed solvent by the action of silver nitrate and a reducing agent, and the grain size of the preparation is about 10 nanometers. The nano silver particle provided by the invention has outstanding dispersion, and the product of the invention can be used as a medical material for treating burns and scalds, and a material for treating skin infection of trauma patients and preventing and treating incision infection after skin and surgical operation incision. The nano silver particle has the excellent properties of broad-spectrum antibiotic property, long-acting property, kill drug resistance and the like.

The invention discloses a nano silver anti-inflammatory gel for treating gynecologic inflammation and a preparation method thereof. The gynecologic inflammation is a common frequently-occurring disease; and an ideal effect cannot be easily obtained from the traditional therapeutic drug. 1000g of gel is prepared from 100-200 PPM of nano silver powder, 2-3g of camellia oil, 2-3g of eucalyptus oil, 0.002-0.008g of sodium dodecyl sulfate, 10-20g of propolis, 4-6g of carbopol, 4-6g of triethanolamine and the balance of pure water. The nano silver anti-inflammatory gel has excellent curative effect on pathogenic microorganism infective inflammation diseases such as gynecologic inflammation, cervicitis, or mixed infection. The gel disclosed by the invention is directly administered through vagina; and the drug directly achieves the focus of infection, and is explicit in targeting property, concise and convenient. The nano silver anti-inflammatory gel has strong inhibiting or killing roles on various pathogens such as gram positive bacteria, gram negative bacteria, mould, trichomonad and the like by experimental and clinical validation. The nano silver anti-inflammatory gel has good curative effect on colpitis, cervicitis, annexitis and pelvic inflammation caused by lactobacillus, mycete, candida albicans, HPV (human papillomavirus) and the like.

The invention discloses an antibacterial mildew-resistant corrosion-resistant flame-resistant integrated nanometer functional paint, and preparation method and applications thereof. The antibacterialmildew-resistant corrosion-resistant flame-resistant integrated nanometer functional paint is mainly composed of a corrosion-resistant flame-resistant epoxy priming paint and an antibacterial mildew-resistant wear-resistant polyurethane finish paint. According to the preparation method, an intumescent flame retardant and graphene are added into the epoxy priming paint as flame retardation auxiliary agents; the paint flame resistance is improved greatly through the cooperative effect of the nanometer material and the intumescent flame retardant; nanometer silver antiseptic and hyperbranched polyethylene imide quaternary ammonium salt nanometer particles are added into the polyurethane finish paint as antibacterial auxiliary agents, and the paint antibacterial mildew-resistant performance isimproved through the cooperative effect of the nanometer silver antiseptic and the hyperbranched polyethylene imide quaternary ammonium salt nanometer particles. The reasonable combination of the priming paint with the finish paint is capable of improving the paint corrosion resistance further. The antibacterial mildew-resistant corrosion-resistant flame-resistant integrated nanometer functionalpaint possesses the antibacterial performance, the mildew resistance, the flame resistance, the corrosion resistance, and the wear resistance, is wide in application range, is simple in preparation method, is convenient in operation, and is convenient for large scale applications.

The invention discloses a compound plant essential oil micro-capsule preparation and a preparation method thereof, and belongs to the field of animal feed additives. The compound plant essential oil micro-capsule preparation comprises the following ingredients in percentage by mass: 5-30% of plant essential oil, 30-60% of auxiliary materials, 0-1% of pigments, and the balance of a carrier material; and the plant essential oil is a compound composed three essential oils, including carvacrol, thymol and cinnamaldehyde, at random proportions. The compound plant essential oil micro-capsule preparation disclosed by the invention is light red to red round smooth solid particles, wherein the micro-capsules are smooth at surface, uniform in color and luster, and 200-800 microns in particle size; and thus, the compound plant essential oil micro-capsule preparation has relatively good fluidity and dispersibility so that uniform mixing of feeds is facilitated so as to avoid sticking of the feedson walls when the feeds are used for feeding. And thus, the compound plant essential oil micro-capsule preparation is capable of reducing consumption loss, and increasing breeding benefits.

The invention discloses a medical antibacterial hydrogel dressing and a preparation method thereof. The hydrogel dressing is prepared from the following raw materials: thiolated chitosan, sodium glycerophosphate hydrate, carbomer, oxidized sodium alginate, L-arginine ethyl ester dihydrochloride, theophylline, glycine fumarate, L-carnitine powder, stachyose, hydroxyethyl methylacrylate, tri(propylene glycol) diacrylate, ginkgo flavonol, polyhexamethylene biguanidine, povidone iodine, alcoholic extract of herba andrographitis, triethanolamine and de-ionized water. The hydrogel dressing prepared by the invention can achieve inhibitory rates 91% and 94% or above on pseudomonas aeruginosa and staphylococcus aureus separately, and the hydrogel dressing has a broad-spectrum antibacterial property and a durable antibacterial effect; and in addition, the hemostasis time of the dressing is just 9-18s and healing time is shorter than 10d, and healed regenerated skin has a smooth and delicate surface; therefore, the hydrogel dressing is excellent in wound healing capacity and quite good in healing effect.

This invention is a method how to make a paper has bacteria-static function. It uses the nanopowder it has the function of bacteria-static combine with paper to produce antibiotic paper. The elementary nanopowder has bacteria-static function because they have been combined with quarter-nary ammonic-salt or quarter-nary phosphate antibiotic polymer use the co-valence decorated method. Put the antibiotic nanopowder into paper pulp according to the ratio of 0.01-0.1% then make up a bacteria-static paper.

The invention relates to sorghum and quinoa multi-grain bread made through a liquid fermentation method, and a processing method thereof, and belongs to the field of agriculture product processing. The bread is made from 20-30 parts of whole sorghum flour, 10-20 parts of whole quinoa flour, 50-70 parts of high gluten wheat flour, 0.8-1.2 parts of dry yeast, 2-4 parts of butter, 2-4 parts of skim milk powder, 0.3-0.5 part of bread improver, 4-8 parts of egg liquid, 10-20 parts of milk, 0.6-0.8 part of table salt, 2-4 parts of white granulated sugar, 25-30 parts of water and 2-3 parts of beer. The made bread is good in quality, reasonable in raw material matching, comprehensive in nutrition, mellow in taste and long in refreshing time, and fragrance is stronger due to the addition of beer.

The invention relates to a synthesis method of metal nanometer particle and polysaccharide polymer composite material, which comprises subjecting solid phase polysaccharide polymers to oxidation reduction reaction in complex solution of liquid phase metal salt, reducing metallic ions in the solution into metallic atoms under the action of reducing agent, coordinating with hydroxyl on the polysaccharide polymer and and growing into atom clusters and nano particles. The physical chemical properties of the polysaccharide polymers can be substantially improved.

The invention relates to a human body lubricant which mainly comprises a wetting agent, a nutrient, a thickening agent, a disinfectant and deionized water. The invention also relates to a method for preparing the human body lubricant, and the method comprises the following steps: mixing the components in sequence; uniformly stirring; and filling. The human body lubricant has proper stickiness, is not easily dried, can be cleaned easily and has the advantages of broad-spectrum antibacterial property, bactericidal effect, no toxicity or irritation, non sensitization and safety in use.

The invention relates to the field of stainless steel materials, in particular to medical anti-infection stainless steel. The stainless steel comprises the following chemical compositions in percentage by weight: less than or equal to 0.08 percent of C, less than or equal to 1.00 percent of Si, less than or equal to 2.00 percent of Mn, 8-16 percent of Ni, 16-20 percent of Cr, 3.0-5.0 percent of Cu, 0-4.0 percent of Mo and the balance of Fe. The content of impurity elements in steel is consistent with a corresponding requirement in a medical stainless steel national standard. After special thermal treatment on the stainless steel, bar-shaped or particulate copper-rich precipitated phases are uniformly dispersed and distributed in a stainless steel substrate, so that the stainless steel is endowed with an anti-bacterial-infection function. The medical anti-infection stainless steel has a unique anti-bacterial-infection function and can be widely applied to various stainless steel medical instruments used in the medical clinical fields of the department of orthopedics, the department of stomatology, cardiovascular brackets and the like; and the problems of bacterial infection and the like caused by medical instruments such as medical stainless steel and the like in the prior art are solved.

The invention discloses a preparation method of nano-silver / diatomite / calcium alginate composite antibacterial hydrogel microspheres. The preparation method comprises the following steps: purifying natural diatomite; mixing silver nitrate, absolute ethyl alcohol and the obtained diatomite, and performing ultrasonic dispersion to obtain dispersion liquid A; mixing sodium hydroxide and absolute ethyl alcohol to obtain a solution B; slowly adding B into A, conducting uniform stirring and mixing, successively performing centrifuging, washing and centrifuging, and then carrying out drying to obtain diatomite-based nano-silver particles; dissolving sodium alginate in deionized water, adding the obtained diatomite-based nano-silver particles, and conducting uniform stirring and dispersing; and dropwise adding the obtained dispersion liquid into an aqueous solution of calcium chloride at a constant speed, and filtering out cured gel beads to obtain the composite antibacterial hydrogel microspheres. On the basis of having antibacterial effect, the obtained antibacterial microspheres effectively slow down overflow of silver ions, the fillers have good biocompatibility, and the microspheres have high mechanical strength, are beneficial to recycling and provide guarantee for long-acting sterilization of domestic water.

The invention relates to a cleanser and discloses an acne-removal cleanser and a preparation method thereof. The acne-removal cleanser is prepared from 0.45%-2.52% by weight of a guava extract and the balance of auxiliaries. The cleanser can be further prepared from the guava extract, PEG-160 sorbitan triisostearate, glycerin and the like. The acne-removal cleanser has remarkable efficacy on treatment of whelks, acne and the like, the whelks, the acne and the like cannot relapse after the cleanser is used for a long time, and skin is fine and glossy and has good elasticity.

The invention provides a high-strength-plasticity antibacterial high-entropy alloy and a preparation method thereof. The expression of the high-strength-plasticity antibacterial high-entropy alloy isAl<x>CoCrCu<y>FeNi, wherein x is greater than or equal to 0 and less than or equal to 0.5, y is greater than or equal to 0.1 and less than or equal to 1.2, and the ratio of the elements is the molar ratio. The preparation method comprises the steps of adjusting a vacuum arc furnace to an anaerobic atmosphere, adding Al, Co, Cr, Cu, Fe and Ni in sequence, controlling the vacuum degree in the furnace, introducing argon at specific air pressure, and conducting smelting and casting to obtain the high-strength-plasticity antibacterial high-entropy alloy. The antibacterial high-entropy alloy prepared through the method has the broad-spectrum antibacterial performance, also has the high strength and plasticity and can meet the special requirements in some severe environments.

The invention discloses a ternary metal organic framework sterilizing material and a preparation method and application thereof. The method comprises the following steps: taking a solvent as a reaction medium, uniformly mixing nickel acetate, cobalt acetate, ferrous acetate and 2-aminoterephthalic acid, and reacting for 1-3 hours at room temperature to obtain the nano flower-shaped ternary metal organic framework sterilizing material. The ternary metal organic framework sterilizing material disclosed by the invention is in a nano flower shape, has a good sterilizing effect, and has dual effects of direct sterilization of nickel, cobalt and iron and decomposition catalytic sterilization of H2O2. Compared with the prior art, the ternary metal organic framework sterilizing material disclosedby the invention combines a metal organic framework and heterogeneous Fenton-like reaction to achieve the aim of efficient synergistic sterilization, and the dosage of the sterilizing material and H2O2 is effectively reduced, so that the toxic and side effects are reduced, and the utilization rate of the sterilizing material and H2O2 is improved.

The invention discloses a preparation method of antibacterial polyester fabrics and belongs to the technical field of textile materials. The preparation method comprises the following steps: after sequentially carrying out continuous modification treatment on polyester fabric original samples including clothing, tablecloth and curtains by virtue of a Na2CO3 solution, a mixed solution of alizarin red and sodium cyclamate, a mixed solution of aniline, hydrochloride and ammonium persulfate, a mixed solution of DL-mercaptosuccinic acid and D-penicillamine and a nano-silver solution, putting the treated polyester fabric original samples into a silver tetrafluoroborate aqueous solution, carrying out reaction at room temperature for 30 minutes, washing the product by a great deal of water, and drying at the temperature of 40 DEG C to obtain the antibacterial polyester fabrics. The antibacterial polyester fabrics prepared by the preparation method have the broad-spectrum antibacterial property, are good in antibacterial effect particularly on staphylococcus aureus, bacillus subtilis, Escherichia coli and pseudomonas aeruginosa, are strong in persistence and have the single-strain inhibitory rate reaching 95-99.7 percent after being washed for 30 times.

The invention relates to a preparation method of a nanometer preservative containing composite extracts and the corrosion resistance application of the nanometer preservative. According to the method disclosed by the invention, on the basis that composite clove / cinnamon / fresh ginger extracts (1:1-2:1-2) are extracted and compounded, the nanometer preservative containing the composite extracts is compounded, and the particle diameter can reach 8.69nm. The combination of the added 0.5g / kg of the nanometer preservative with the condition of disinfection for 15-20 minutes at the temperature of 85 DEG C can be applied to corrosion resistance and bacteriostasis of sauce products, so that the products can have the storage shelf life of one year. The nanometer preservative containing the composite clove / cinnamon / fresh ginger extracts, disclosed by the invention, has extensive bacteriostasis scopes, and has high safety, waster solubility and stability. The combination of the composite natural agent for corrosion resistance and bacteriostasis with flexible disinfection conditions can well maintain the nutrient substances and the flavor characteristics of products, and wide application prospects.

The invention provides an aromatic antibacterial microcapsule with a carbon skeleton and a preparation method thereof. In the invention, essence oil is used as a core material, natural polymer gelatinand Arabic gum are used as wall materials, and the antibacterial carbon skeleton aromatic microcapsule is prepared through a complex coacervation method. The preparation method comprises the following steps: firstly, treating plant pollen, calcining the pollen into a hollow carbon skeleton, and supporting silver nanoparticles on the surface of the carbon skeleton through a sol supporting method;then dispersing and coating the core material essence essential oil in the hollow carbon skeleton; and finally, gradually depositing gelatin and Arabic gum on the surface of the carbon skeleton to coat the core material, and adding a cross-linking agent for curing the material to form the long-acting slow-release aromatic antibacterial microcapsule with a stable structure. The carbon skeleton microcapsule prepared by the invention has aromatic odor and antibacterial property, and has the characteristics of environmental protection, no toxicity, slow release, bacteriostasis, good mechanical strength, good heat resistance and the like. According to the preparation method, the application stability of the essence microcapsules is improved, and the service life is prolonged.

The invention provides a method for preparing underwear with an antibacterial effect by utilizing an electrostatic spinning method. The method comprises the following steps: preparing hydrophobicallymodified chitosan by adopting chitosan; dissolving a natural antibacterial agent into the chitosan solution, and crosslinking glutaraldehyde to obtain a hydrophobically modified chitosan coated natural antibacterial agent; adding carboxymethyl chitosan and hydrazine hydrate into silver nitrate, and obtaining a nano-silver antibacterial solution; dispersing the hydrophobic modified chitosan coatednatural antibacterial agent and the nano-silver antibacterial solution into a high-molecular polymer solution, and defoaming to obtain a nano-antibacterial spinning solution; adding the nano-antibacterial spinning solution into electrostatic spinning, and electrospinning a composite nano-antibacterial fiber layer on the surface of the base cloth layer; obtaining the underwear fabric with the antibacterial effect through a hot pressing process; and preparing the underwear with the antibacterial effect. The silver and a natural antibacterial agent are processed into nanofibers and rapidly embedded into the fabric, multiple antibacterial materials are compounded into the nano antibacterial fibers at a time, and the nano antibacterial fibers have high porosity and high specific surface area and bring air and moisture permeability and antibacterial effects.

The invention belongs to the technical field of nano material preparation, and discloses a method of preparing antimicrobial nanofiber complex film of polyvinyl alcohol / muramidase / ethylene diamine tetraacetic acid (EDTA) from electrostatic spinning and the application of the antimicrobial nanofiber complex film of the polyvinyl alcohol / muramidase / EDTA. The preparation comprises the following steps: solving the polyvinyl alcohol in distilled water, carrying out magnetic stirring and obtaining polyvinyl alcohol solution; cooling the obtained polyvinyl alcohol solution to room temperature, and adding the muramidase and the EDTA to the polyvinyl alcohol solution; carrying out magnetic stirring to obtain spinning solution; and sucking the spinning solution into an injection syringe, controlling the flow speed of the injection syringe, spinning, drying in vacuum, and obtaining the antimicrobial nanofiber complex film with the biological activity. The antimicrobial nanofiber complex film with the biological activity has obvious bacteriostasis to escherichia coli and staphylococcus aureus. The staphylococcus aureus has good toughness and low toxicity features, and has wide application prospect in the field of food packaging materials and biological materials.

The invention relates to bacillus velezensis as well as a culture method and application thereof. The endophytic antagonistic strain bacillus velezensis TA-3-BV of sweet cherries is found for the first time, and the nucleotide sequence of 16S rDNA of the bacillus velezensis TA-3-BV is shown as SEQ ID NO.1. The bacillus velezensis TA-3-BV has broad-spectrum antibacterial activity on sweet cherries,has an inhibition effect on various pathogenic fungi and has an excellent inhibition effect on sweet cherry soft rot pathogenic bacteria rhizopus stolonifer, and the inhibition rate can reach 69.82 + / -1.43%. Besides, the bacillus velezensis TA-3-BV has a relatively good inhibition effect on postharvest decay of the sweet cherries, the decay process of the fruits can be delayed, the decay of the fruits is delayed by 2-3 days, and a novel method is provided for postharvest preservation and fresh keeping of the sweet cherries.