Preparation of antimicrobial nanofiber complex film with biological activity and application thereof

A nanofiber and biologically active technology, applied in the direction of non-woven fabrics, textiles and papermaking, etc., can solve the problems of difficult action of lysozyme and unsatisfactory antibacterial effect, and achieve good toughness, low toxicity, and low toxicity biocompatibility Sexuality and broad application prospects

Inactive Publication Date: 2013-05-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the peptidoglycan layer of Gram-positive bacteria is located on the outer layer of the cell wall, lysozyme has a good antibacterial effect on most Gram-positive bacteria; while the peptidoglycan layer of Gram-negative bacteria is located on the outer layer of the cell membrane. The middle layer is blocked by lipopolysaccharide, an outer membrane substance, which makes it difficult for lysozyme to act on the peptidoglycan layer in the middle, so the antibacterial effect of lysozyme on most Gram-negative bacteria is not ideal

Method used

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  • Preparation of antimicrobial nanofiber complex film with biological activity and application thereof
  • Preparation of antimicrobial nanofiber complex film with biological activity and application thereof
  • Preparation of antimicrobial nanofiber complex film with biological activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Weigh 6g of polyvinyl alcohol and dissolve it in 100mL of distilled water, and magnetically stir for 4 hours at a temperature of 80°C to obtain a polyvinyl alcohol solution;

[0029] (2) After the polyvinyl alcohol solution obtained in step (1) is cooled to room temperature, add egg white lysozyme (purchased from American Amresco Company) in a proportion of 2% of the mass of the polyvinyl alcohol solution, and add EDTA at the same time; stir magnetically at room temperature for 1 h , to obtain a spinning solution; the concentration of the EDTA in the spinning solution is 4mmol / L;

[0030] (3) Inhale the spinning solution obtained in step (2) into a 20mL syringe, install a needle with a diameter of 0.6mm, adjust the distance from the needle to the receiver to 18cm, adjust the voltage at 15kV, and control the flow rate of the spinning solution at 0.19mL / h. silk for 4 hours, and then vacuum-dried at 45°C for 2 hours to obtain a 6%PVA+2%Lysozyme+4mmol / LEDTA nanofiber co...

Embodiment 2

[0032] (1) Weigh 8g of polyvinyl alcohol and dissolve it in 100mL of distilled water, and magnetically stir for 4 hours at a temperature of 80°C to obtain a polyvinyl alcohol solution;

[0033] (2) After the polyvinyl alcohol solution obtained in step (1) is cooled to room temperature, add egg white lysozyme (purchased from American Amresco Company) in a proportion of 5% of the mass of the polyvinyl alcohol solution, and add EDTA at the same time; stir magnetically at room temperature for 1 h , to obtain a spinning solution; the concentration of the EDTA in the spinning solution is 4mmol / L;

[0034] (3) Inhale the spinning solution obtained in step (2) into a 20mL syringe, install a needle with a diameter of 0.6mm, adjust the distance from the needle to the receiver to 12cm, adjust the voltage at 15kV, and control the flow rate of the spinning solution at 0.17mL / h. Spinning for 4 hours, and then vacuum drying at 45°C for 2 hours to obtain 8%PVA+5%Lysozyme+4mmol / LEDTA nanofiber...

Embodiment 3

[0036] (1) Weigh 6g of polyvinyl alcohol and dissolve it in 100mL of distilled water, and magnetically stir for 4 hours at a temperature of 80°C to obtain a polyvinyl alcohol solution;

[0037] (2) After the polyvinyl alcohol solution obtained in step (1) is cooled to room temperature, add egg white lysozyme (purchased from American Amresco Company) in a proportion of 2% of the mass of the polyvinyl alcohol solution, and add EDTA at the same time; stir magnetically at room temperature for 1 h , to obtain a spinning solution; the concentration of the EDTA in the spinning solution is 6mmol / L;

[0038] (3) Inhale the spinning solution obtained in step (2) into a 20mL syringe, install a needle with a diameter of 0.8mm, adjust the distance from the needle to the receiver to 18cm, adjust the voltage at 15kV, and control the flow rate of the spinning solution at 0.18mL / h. Spinning for 4 hours, and then vacuum drying at 50°C for 2 hours to obtain 8%PVA / 2%Lysozyme / 6mmolEDTA nanofiber c...

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Abstract

The invention belongs to the technical field of nano material preparation, and discloses a method of preparing antimicrobial nanofiber complex film of polyvinyl alcohol / muramidase / ethylene diamine tetraacetic acid (EDTA) from electrostatic spinning and the application of the antimicrobial nanofiber complex film of the polyvinyl alcohol / muramidase / EDTA. The preparation comprises the following steps: solving the polyvinyl alcohol in distilled water, carrying out magnetic stirring and obtaining polyvinyl alcohol solution; cooling the obtained polyvinyl alcohol solution to room temperature, and adding the muramidase and the EDTA to the polyvinyl alcohol solution; carrying out magnetic stirring to obtain spinning solution; and sucking the spinning solution into an injection syringe, controlling the flow speed of the injection syringe, spinning, drying in vacuum, and obtaining the antimicrobial nanofiber complex film with the biological activity. The antimicrobial nanofiber complex film with the biological activity has obvious bacteriostasis to escherichia coli and staphylococcus aureus. The staphylococcus aureus has good toughness and low toxicity features, and has wide application prospect in the field of food packaging materials and biological materials.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and in particular relates to a method for preparing antibacterial polyvinyl alcohol / lysozyme / EDTA (PVA / Lysozyme / EDTA) nanofiber composite film with biological activity by electrospinning and its application. Background technique [0002] Lysozyme (Lysozyme), also known as muramidase, its main antibacterial mechanism is to act on the β-1,4 between N-acetylmuramic acid (NAM) and acetylglucosamine (NAG) of peptidoglycan molecule The glycosidic bond makes the bacterial cell wall loose, loses the protective effect on the cell, and finally causes the cell to lyse and die. Since the peptidoglycan layer of Gram-positive bacteria is located on the outer layer of the cell wall, lysozyme has a good antibacterial effect on most Gram-positive bacteria; while the peptidoglycan layer of Gram-negative bacteria is located on the outer layer of the cell membrane. The middle layer is blocked by li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/4309D04H1/728D01F6/50D01F1/10
Inventor 吴虹朱定和宗敏华娄文勇
Owner SOUTH CHINA UNIV OF TECH
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