41 results about "Bacteriolytic enzyme" patented technology

The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.

The invention discloses enzyme supplementation gum which is composed of 0.1%-0.3% of muramidase, 18%-22% of aluminium hydroxide, 2%-3% of vitamin C, 0.6%-1% of citric acid, 2%-3% of glycerinum, 20%-30% of gum base, 40%-45% of sorbitol, 4%-6% of mannitol, 0.5%-1% of essence and 6%-10% of water. According to the enzyme supplementation gum, the biological muramidase and the citric acid are added, the pH value of the gum can keep the activity of the muramidase to the maximum degree, and bacteria resistance and bacteriostasis, dental calculus prevention, caries prevention, ozostomia relieving, dental plaque inhibition and remarkable improvement of gingival bleeding are conducted on the aspect of biology; the aluminium hydroxide and the vitamin C can assist the muramidase in removing dental plaque and stain, the effect is more remarkable, gingival bleeding is inhibited, and the gum can taste sour and sweet and delicious; the teeth can be healthy and white and breath can be fresh through long-term use of the enzyme supplementation gum.

The invention provides a fusion protein. The fusion protein is formed by connecting an anti-iysozyme antibody or a variable region fragment of the anti-iysozyme antibody with a horseradish peroxidase reporter gene protein in series, the invention further provides a preparation method and an application of the fusion protein. The fusion protein is used for immunoassay which takes an immunolabelling technique as a basis, so that good biological activities of enzymes and the antibody are kept, and the detection efficiency is higher.

The invention relates to a bio-enzyme corrosion inhibitor for slowing down the carbon steel corrosion in a circulating water system. The bio-enzyme corrosion inhibitor is prepared from the following ingredients by weight portion: 1 portion of lipase and 1.5-2.5 portions of muramidase. The activity of the lipase is larger than 40000u/g, and the activity of the muramidase is larger than 10000u/g. The invention also discloses a method for slowing down the carbon steel corrosion in the circulating water system with the bio-enzyme corrosion inhibitor. The bio-enzyme corrosion inhibitor is formed by compounding the lipase and the muramidase according to a certain mass ratio and has the advantages of less dosage, convenience in use, wide sources, no toxicity, no pollution, good biodegradability and the like. The bio-enzyme corrosion inhibitor can generate a black dense matterl, namely ferroferric oxide on a carbon steel surface to prevent oil leaked from circulating water from further corroding carbon steel equipment. Meanwhile, the lipase in the bio-enzyme corrosion inhibitor can play a role of degrading diesel fuel, and therefore the corrosion inhibition effect is further increased.

The invention relates to a complex enzyme preparation for a leaching tobacco material and an enzyme leaching method, and belongs to the technical field of regenerated tobacco processing. The active component of the complex enzyme preparation comprises 0.1-1000U of lysozyme, 1-1000 U of cellulase, 0.1-1000U of alpha-amylase, 0.1-1000 U of proteolytic enzyme and 0.1-1000U of pectinase, wherein the amount is the adding amount in per gram of tobacco material. The temperature in leaching is controlled at 30-60 DEG C, and the liquor ratio is 1:5-10; the enzyme preparation is added to be stirred for 1-4 hours; and after solid-liquid separation, solid phase is taken and water is further added according to original solid weight for circularly extraction for 1-2 times. According to the invention, mesophyll cell and cabo fiber structures, composition, physical properties and the like of tobacco are adequately considered, combined wall breaking effects of lysozyme, pectinase and cellulase are introduced, and enzyme catalytic action of proteolytic enzyme, alpha-amylase and cellulase for degrading biomacromolecule is considered, so that the compact structure of tobacco and straw is dredged, leach of inclusions of materials is increased, the dissolution rate is enhanced and the quality of the extracting liquid is improved.

The present invention discloses a bacteriostatic protein and an encoding gene and an application thereof and a strain method. Chicken lysozyme is used as a starting template, an amino acid sequence ofscorpion-derived antimicrobial peptide IsCT is superposed to an N terminal of the chicken lysozyme protein through rational design, and meanwhile, an antioxidant peptide joint and an amino acid jointare directionally introduced to obtain a mutant LyIc; then according to a codon preference of pichia pastoris, a whole-gene synthesis technology is adopted to obtain a gene lysIs corresponding to themutant LyIc and a nucleotide sequence of the lysIs is shown as SEQ ID NO.1; then the lysozyme mutant gene lysIs is linked into an expression vector pPICZ alpha A, the vector is transferred into pichia pastoris X33, the recombinant microorganisms are subjected to fermentation culture and the lysozyme mutant LyIc is separated and purified; and finally, bacteriostatic effects of the lysozyme mutantLyIc and original lysozyme on common pathogenic bacteria are compared and analyzed. The lysozyme mutant LyIc effectively improves the bacteriostatic effect on common pathogenic bacteria (escherichia coli, salmonella and staphylococcus aureus) and lays foundation for wide application of the chicken lysozyme.

The invention provides a biological enzyme composite disinfectant as well as a preparation method and application thereof. The method comprises the following steps: dissolving folium eriobotryae, bamboo leaves, dandelion and houttuynia cordata in deionized water, heating the liquid to 100 DEG C, boiling the liquid for 30-60 minutes, and cooling the liquid; sequentially adding lysozyme and the plant extracting solution into the cooled water, and magnetically stirring the solution for 1-2 hours until the solution is uniformly mixed; and adding spices as required, uniformly stirring and sub-packaging the products. The enzyme composite disinfectant has good antibacterial and bactericidal effects, and the antibacterial and bactericidal rate reaches 99.9%; the preparation process is relatively simple and easy to operate.

The embodiment of the invention discloses a biological deodorant for pets and a preparation method thereof. The biological deodorant for pets comprises the following components: a plant extracting solution and a biological active enzyme, wherein the mass ratio of the plant extracting solution to the biological active enzyme is (2-10): (1-2); the plant extracting solution comprises a zanthoxylum bungeanum fruit extracting solution, a radix sophorae flavescentis extracting solution, an aloe barbadensis leaf extracting solution, a citrus peel extracting solution, a pseudolarix amabilis root and bark extracting solution and a persimmon fruit extracting solution; the bioactive enzyme comprises lipase, desulfhydrylase, oxidoreductase and lysozyme. According to the embodiment of the invention, the problems of low deodorization efficiency and incomplete deodorization of the existing deodorization product can be effectively solved, meanwhile, the biological deodorant for pets provided by the invention takes the plant extract liquid as a main component, and the plant extract liquid reacts with odor gas molecules to fundamentally remove odor and inhibit the reproduction and diffusion of bacteria after pets urinate and defecate, so that the biological deodorant for pets has a good deodorization effect. The pet food is safe, environment-friendly, free of secondary pollution and capable of meeting the requirement that the living environment of pets and owners thereof is odorless.

The invention discloses a method for constructing high-internal-phase emulsion gel based on a dihydromyricetin/egg lysozyme compound emulsifier, and belongs to the technical field of food. The method comprises the following steps: mixing dihydromyricetin and egg lysozyme and directly adding a mixture into water to serve as a water phase; taking edible oil as an oil phase; mixing the oil phase and the water phase, shearing at a high speed, and standing at room temperature to obtain the high-internal-phase emulsion gel. The prepared high-internal-phase emulsion gel is high in safety, good in stability and rich in natural antioxidant dihydromyricetin and edible bacteriostatic agent egg lysozyme, and can be used as a solid fat substitute and a functional component delivery body to be applied to the field of food.