19 results about "Bacteriolytic enzyme" patented technology

A general formula for lysins based on which the activity and functionality are further screened and 24 lysins are identified and further investigated, nine of which are characterized. These lysins exhibit potent bactericidal activity, capable of lysing multiple species of Staphylococcus, Streptococcus, and / or Enterococcus; demonstrate robust environmental resistance and stability, retaining activity under varying temperatures, NaCl concentrations, pH levels, and in immune serum; and effectively treat localized and systemic infections. These lysins and their variants are promising as antimicrobial agents for eradicating bacteria, preventing bacterial infections, or treating bacterial infections (caused by genera Staphylococcus, Streptococcus, and / or Enterococcus).

The invention relates to the technical field of feed additives, and discloses a composite functional feed additive as well as a preparation method and application thereof. The composite functional feed additive comprises the following components in parts by mass: 20-80 parts of antibacterial peptide, 1-15 parts of tannic acid and 10-100 parts of a plant fiber carrier. According to the additive, expressions of key antioxidant genes such as sod1, cat, gst and gr in the liver are remarkably up-regulated on the molecular level, and it is indicated that an Nrf2 signal channel is successfully activated, so that the endogenous antioxidant defense capacity of cells is enhanced from the source; in the immune level, the additive can effectively regulate inflammatory response, remarkably down-regulate expression of proinflammatory factors and apoptosis genes, up-regulate expression of anti-inflammatory factors and improve the activity of immune enzymes such as lysozyme, so that excessive inflammation and cell apoptosis are inhibited, and the immune homeostasis is maintained; when the feed is used for aquaculture, the oxidative stress of livers of aquatic animals can be remarkably relieved, cell apoptosis is inhibited, and the disease resistance is improved.

The invention belongs to the technical field of polymer protective materials. The invention provides a pair of self-repairing antibacterial allergy-free butyronitrile gloves, the base material of which is prepared from the following components in parts by weight: 100 parts of nitrile rubber, 3 to 5 parts of dynamic cross-linking agent, 4 to 6 parts of lysozyme-chitosan composite microspheres, 12 to 15 parts of plasticizer, 1.0 to 1.2 parts of vulcanizing agent, 0.8 to 1.0 part of accelerant, 1.0 to 1.2 parts of stabilizer and 200 to 220 parts of deionized water, the dynamic cross-linking agent is dithiodipropionic acid; 0.5-1.0 part of hyaluronic acid moisturizing coating is coated on the inner surface of the base material. Aiming at the technical defects that the existing butyronitrile gloves are easy to damage, an antibacterial agent is easy to lose and the sensitization is high, a disulfide bond dynamic cross-linked network is constructed in a nitrile rubber matrix, a lysozyme-chitosan composite microballoon enzyme catalytic antibacterial system is introduced, and a phytosterol plasticizer and a hyaluronic acid inner coating are matched, so that the anti-allergenicity butyronitrile gloves are prepared. The coordination of triple functions of self-repairing, long-acting antibacterial and low sensitization is realized.

The solid formulation (tablet) prepared from a specific liquid mixture containing bacteriophages and endolysins active against the important pathogens Staphylococcus aureus and Pseudomonas aeruginosa as active ingredients is therapeutically applicable and dose-stable for the treatment of infections caused by these antibiotic-resistant pathogens. It also allows the bacteriophages and their enzymes to be maintained and stored at relatively high temperatures and other conditions that are unfavorable for these active ingredients.

Unglycosylated lysostaphin variant protein, nucleic acid molecule, vector and host cell, as well as a method for production of unglycosylated lysostaphin variant protein in a yeast expression system are provided. The proteins are produced in a Pichia pastoris expression system and have been shown to have activity equivalent to wild-type lysostaphin. The lysostaphin variant proteins can be used as therapeutic proteins for treatment of diseases such as Staphylococcus aureus infection.

The invention belongs to the technical field of non-woven fabrics, and particularly relates to bio-enzyme bacteriostatic cloth and a preparation method thereof, a bacteriostatic base material is bio-enzyme, the cloth base material comprises non-woven fabrics, spunlace fabrics, wiping fabrics or hot air fabrics and the like, the bio-enzyme comprises one or more of lysozyme or protease, and the loading capacity of the bio-enzyme on the cloth base material is 1g / m < 2 >-5g / m < 2 >; the preparation method comprises the following steps: 1 to 4, selecting a biological enzyme, dissolving the biological enzyme in a buffer solution, adding a certain amount of a stabilizer and a protective agent to prepare a biological enzyme solution, and uniformly stirring the biological enzyme solution for later use; the cloth base material is placed on Kiss Lora liquid feeding equipment, parameters are set, the Kiss Lora liquid feeding equipment is started, and the biological enzyme solution is evenly fed to the surface of the cloth base material. According to the preparation method disclosed by the invention, the biological enzyme is loaded on various cloth products through a Kiss Lora process, and an optimized drying technology is combined, so that the biological enzyme antibacterial cloth with efficient antibacterial performance is prepared.

The present invention relates to a live attenuated Gram-negative bacterium comprising a modified hlyCABD operon, wherein the modified hlyCABD operon is split into a first segment and a second segment, the first segment being operably linked to a first independently controlled promoter, wherein the first independently controlled promoter is a strong constitutive promoter or a strong vacuole-induced promoter, and the second segment being operably linked to a second independently controlled promoter, wherein the second independently controlled promoter is a strong vacuole-induced promoter, and wherein the first segment comprises a heterologous polynucleotide encoding a lysin upstream of a hlyAs translocation sequence, wherein the heterologous polynucleotide encoding one or more cargo molecules replaces a hlyA gene, and wherein the second segment comprises hly genes involved in secretion.

An anhydrous composition comprising an endolysin derived from a Staphylococcus aureus phage and mannitol. The present invention relates to an anhydrous composition comprising (i) at least one endolysin derived from a Staphylococcus aureus phage; and (ii) mannitol, the endolysin comprising a protein sequence having at least 80% sequence identity with an amino acid sequence selected from the group consisting of the reference amino acid sequences SEQ ID NO: 1 and SEQ ID NO: 2. It also relates to a composition comprising, in a physiologically acceptable medium, at least one anhydrous composition of the invention.It also covers the use of such a composition to prevent and / or treat a skin disorder related to Staphylococcus aureus colonization in an individual in need, and a non-therapeutic cosmetic process for the care of keratinous materials comprising at least one step of topical application of such a composition to said keratinous materials. Finally, it covers a process for stabilizing an endolysin derived from a Staphylococcus aureus phage. Figure for the abstract: None.

This invention relates to the field of nonwoven fabric preparation technology, specifically to a biochemically modified antibacterial nonwoven fabric and its preparation method. This invention modifies nano-zinc oxide and nano-titanium dioxide using the silane coupling agent KH-570, improving the dispersibility of the nanoparticles. Under light or contact conditions, the nanoparticles release reactive oxygen species to disrupt bacterial cell membranes and inhibit their metabolism, thereby giving the antibacterial modified polypropylene masterbatch stable antibacterial properties. Furthermore, this invention stabilizes the loading through a polydopamine coating, thus stabilizing the antibacterial properties of lysozyme. Lysozyme specifically hydrolyzes the peptidoglycan layer of bacterial cell walls, leading to bacterial lysis. Thiourea dioxide, as a reducing agent, maintains lysozyme activity, preventing oxidative inactivation, while simultaneously inhibiting bacterial metabolic enzyme activity. Through the synergistic effect of inorganic and biological dual-effects, this invention can cover Gram-positive bacteria, Gram-negative bacteria, and fungi, thereby improving the antibacterial properties of the nonwoven fabric to a certain extent.

Disclosed herein is a non-chromosomal dynamic activation system (ADAS) derived from a parent bacterial cell, the ADAS comprising at least one genetic loss-of-function modification in a lytic enzyme to increase the stability of the ADAS. Also disclosed is a method for disrupting sporulation in the parent bacterial cell in combination with a lytic enzyme deletion or other loss-of-function mutation.

Disclosed herein is a non-chromosomal dynamic activation system (ADAS) derived from a parent bacterial cell that contains at least one loss-of-function genetic modification in a lytic enzyme to increase the stability of the ADAS and at least one gene loss-of-function in a protease to improve cargo expression, with or without an additional engineered cell wall-embedded anchor structure to optimize cargo presentation.

The invention discloses a low-sodium oil-rich salted duck egg pickling method and system based on biological enzymolysis, and the method comprises the following steps: enzymolysis pretreatment: carrying out surface modification on eggshells by using lysozyme with a specific pH value and cutin protease compound liquid in cooperation with ultrasonic waves; performing variable-pressure pulsation pickling, injecting a micro-emulsified low-sodium pickling solution prepared from sodium chloride and potassium chloride, and performing pickling by adopting a periodic variable-pressure process; low-temperature balancing; antibacterial film coating; and carrying out sectional variable-temperature baking and curing. According to the method, air holes are dredged through biological enzymolysis, and a micro-emulsification system and a variable-pressure pulsation technology are combined, so that the mass transfer efficiency is improved, and low-sodium pickling is realized; meanwhile, coating and variable-temperature baking processes are matched, so that the breakage rate of eggshells is reduced. The prepared salted duck eggs have the advantages of being low in sodium, healthy, high in yolk oil yield, good in taste and short in production cycle.

The invention relates to the technical field of beer brewing, and discloses a method for prolonging the refreshing time and improving the taste of craft beer by using egg white lysozyme, and the method comprises the following steps: carrying out wort preparation and main fermentation; adding an egg white lysozyme solution prepared by purification into the wine liquid in a stage from the end of main fermentation to the front of filling, and controlling the final concentration to be 15-90mg / L; the wine liquid is subjected to cold storage and after-ripening under the condition of 0-5 DEG C, lysozyme is used for cracking gram-positive putrefying bacteria, and biological preservation is completed under the non-thermal sterilization condition; and filtering and filling after cold storage is finished. According to the method, the specific bacteriostatic action of the high-activity lysozyme is utilized, thermal sterilization and chemical preservatives are replaced by a biological enzyme method, infectious microbes are effectively inhibited, the quality guarantee period is prolonged, meanwhile, damage of high temperature to flavor substances is avoided, the fresh taste and pure flavor of the craft beer are reserved, and the quality guarantee period of the craft beer is prolonged. The problem that fresh keeping and flavor of craft beer are difficult to consider at the same time is effectively solved.

To provide a beverage / food product composition which has an emulsifying function and can produce various food products inhibited in a smell specific to Bacillus natto or hay bacillus though containing a component derived from the bacilli, and reduced in adhesive strength to allow easy taking.SOLUTION: A beverage / food product composition is obtained by subjecting nutrient cells of at least one of hay bacillus and Bacillus natto to enzyme reaction processing with lysozyme or to fermentation processing with microbes (though excluding one obtained by further affecting protein degrading enzymes).SELECTED DRAWING: None

A carbomer hydrogel for maintaining the natural skin microflora and suppressing pathogenic bacteria that contains lytic enzymes and / or highly stable crude / purified Staphylococcus aureus phage lysates with a titer of at least 107 pfu / g of gel and crude / purified Cutibacterium acnes phage lysates with a titer of at least 105 pfu / g of gel.

The application provides a lactobacillus plantarum exopolysaccharide complex and a preparation method and application thereof, and belongs to the technical field of cosmetics. After fermentation of lactobacillus plantarum T1, crude polysaccharide is separated, and zymolytic enzyme is used to obtain a zymolytic system, the crude polysaccharide is added for treatment, modified exopolysaccharide is prepared, mixed with ferulic acid, activated, and then antibacterial peptide and amino-acetylated hyaluronic acid are added and stirred to prepare the lactobacillus plantarum exopolysaccharide complex. The lactobacillus plantarum exopolysaccharide complex prepared by the application has good moisturizing, antibacterial, anti-inflammatory, antioxidant and anti-allergic properties, so that the prepared product has good application and can be used as a safe, non-toxic and non-immunogenic effective cosmetic raw material.

The invention discloses an enzyme and chemical agent combined reverse osmosis membrane cleaning agent as well as a preparation method and application thereof, and belongs to the technical field of water treatment membrane cleaning agents. The invention provides an enzyme and chemical agent combined reverse osmosis membrane cleaning agent which comprises the following components in percentage by mass: 0.05%-0.2% of lysozyme, 0.2%-0.6% of amylase, 0.1%-0.4% of cellulase, 0.2%-0.8% of protease, 1%-3% of a non-oxidizing bactericide, 2%-5% of a chelating agent, 2%-8% of a surfactant and the balance of deionized water. When the reverse osmosis membrane cleaning agent is applied, biological pollution which is difficult to remove on the surface of a reverse osmosis membrane is effectively removed while membrane elements are not damaged and the use of chemical substances is reduced, and the membrane flux is improved.