Bio-enzyme antibacterial cloth and preparation method thereof

Abstract

The invention belongs to the technical field of non-woven fabrics, and particularly relates to bio-enzyme bacteriostatic cloth and a preparation method thereof, a bacteriostatic base material is bio-enzyme, the cloth base material comprises non-woven fabrics, spunlace fabrics, wiping fabrics or hot air fabrics and the like, the bio-enzyme comprises one or more of lysozyme or protease, and the loading capacity of the bio-enzyme on the cloth base material is 1g / m < 2 >-5g / m < 2 >; the preparation method comprises the following steps: 1 to 4, selecting a biological enzyme, dissolving the biological enzyme in a buffer solution, adding a certain amount of a stabilizer and a protective agent to prepare a biological enzyme solution, and uniformly stirring the biological enzyme solution for later use; the cloth base material is placed on Kiss Lora liquid feeding equipment, parameters are set, the Kiss Lora liquid feeding equipment is started, and the biological enzyme solution is evenly fed to the surface of the cloth base material. According to the preparation method disclosed by the invention, the biological enzyme is loaded on various cloth products through a Kiss Lora process, and an optimized drying technology is combined, so that the biological enzyme antibacterial cloth with efficient antibacterial performance is prepared.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of non-woven fabrics, and particularly relates to a bio-enzyme antibacterial fabric and a preparation method thereof. BACKGROUND

[0002] The antibacterial fabric material can be used as a bandage, a medical tool bag, a sanitary core, water jet fabric, wiping cloth, hot air cloth, etc., and is generally manufactured through a high-temperature melt-blowing process. Since the raw materials are directly killed by high-temperature melting, water jetting, etc., the sterilization of the finished product is high.

[0003] At present, people have increasingly high requirements for health and hygiene, and the antibacterial demand of various cloth products in daily life, medical treatment, industry, etc. is extremely urgent. Traditional antibacterial methods, such as chemical agent antibacterial, have problems such as chemical residues, potential harm to the human body and the environment, etc. As a natural, efficient and environmentally friendly catalyst, bio-enzyme has the advantages of strong specificity, mild action conditions and environmental friendliness, and has great potential when applied to the antibacterial treatment of cloth products.

[0004] However, there is currently a lack of an efficient and stable process for loading bio-enzyme on non-woven fabrics, sanitary cores, water jet fabrics, wiping cloths, hot air cloths and other sanitary products to fully exert the antibacterial performance of bio-enzyme. Therefore, the application proposes a bio-enzyme antibacterial fabric and a preparation method thereof. SUMMARY

[0005] The purpose of the application is to provide a bio-enzyme antibacterial fabric and a preparation method thereof. Bio-enzyme is loaded on various cloth products through the Kiss Lora process, and an optimized drying technology is combined to prepare a bio-enzyme antibacterial fabric with high antibacterial performance.

[0006] The technical scheme adopted by the application is as follows:

[0007] A bio-enzyme antibacterial fabric comprises a cloth substrate and bio-enzyme, and the cloth substrate comprises non-woven fabric, water jet fabric, wiping cloth or hot air cloth.

[0008] As an optional solution, the bio-enzyme comprises one or more of lysozyme or protease, and the loading amount of the bio-enzyme on the cloth substrate is 1 g / m 2 ~ 5 g / m 2 .

[0009] A preparation method of a bio-enzyme antibacterial fabric comprises the following steps:

[0010] Step 1, bio-enzyme solution preparation: select bio-enzyme, dissolve in a buffer solution, add a certain amount of stabilizer and protective agent, and prepare a bio-enzyme solution, and stir uniformly for standby;

[0011] Step two, liquid treatment: the online production of cloth substrate through Kiss Lora liquid equipment, set parameters, start Kiss Lora liquid equipment, make the biological enzyme solution uniform liquid on the cloth substrate surface;

[0012] Step three, drying treatment: the cloth substrate after liquid is directly dried, and the drying is carried out under the set temperature and humidity conditions, and the drying time is, until the cloth substrate is dried, the biological enzyme is firmly attached to the cloth substrate, and the antibacterial cloth is prepared, and then it is wound and collected;

[0013] Step four, quality detection: the antibacterial cloth product is detected, and the sample is selected, and the appearance detection, physical property detection and antibacterial property detection are carried out.

[0014] As an optional solution, the concentration of the biological enzyme solution in the step one is 0.5% to 2%.

[0015] As an optional solution, the liquid pressure of the step two is set to 0.1MPa to 0.5MPa, the flow of the biological enzyme solution is set to 5L / h to 20L / h, and the temperature is set to 15℃ to 35℃.

[0016] As an optional solution, the Kiss Lora liquid equipment includes a conveyor belt, a rack, a cloth guide roller, a biological enzyme solution storage tank, a conveying pipeline, a spray head, a liquid tank, a liquid roller, a liquid transfer roller, a water pressing roller, an omega drying air box and a winding roller.

[0017] Among them, the cloth guide roller, the liquid roller, the liquid transfer roller, the water pressing roller and the winding roller are rotatably installed in the rack, and the pipeline, the spray head, the liquid tank and the omega drying air box are fixed in the rack.

[0018] As an optional solution, in the drying process of the step three, a plurality of hot air outlets and circulating air ducts are arranged in the drying area, so that the hot air uniformly blows the surface of the cloth substrate, and a humidity sensor is used to monitor the drying environment humidity in real time.

[0019] As an optional solution, the drying time of the step three is set to 10s to 30s, and the drying temperature is set to 40℃ to 85℃.

[0020] As an optional solution, the appearance detection is observed by online detection, whether the cloth surface is uniform, whether there are stains and foreign matters.

[0021] As an optional solution, the physical property detection includes longitudinal strip tensile strength, longitudinal elongation, transverse strip tensile strength, transverse elongation, water absorption multiple, water absorption amount, water retention capacity and 5ML penetration time.

[0022] The technical effects obtained by the application are:

[0023] The present application loads bio-enzyme on various cloth products by Kiss Lora process, and combines with optimized drying technology, to prepare bio-enzyme antibacterial cloth with high efficient antibacterial performance, which has wide application prospect in medical health, daily life cleaning, industrial production and other fields. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is a front view of a bio-enzyme antibacterial cloth in embodiment one of the present application;

[0025] Figure 2 is a flow chart of a preparation method of a bio-enzyme antibacterial cloth in embodiment two of the present application;

[0026] Figure 3 is a structural schematic diagram of a Kiss Lora liquid feeding device in embodiment two of the present application.

[0027] In the drawings, the component list represented by each reference numeral is as follows:

[0028] 1, cloth guide roller; 2, bio-enzyme solution storage tank; 3, conveying pipeline; 4, spray head; 5, liquid feeding tank; 6, liquid feeding roller; 7, liquid feeding transfer roller; 8, water pressing roller; 9, Omega drying air box; 10, winding roller. DETAILED DESCRIPTION

[0029] In order to make the purpose and advantages of the present application more clear and explicit, the present application is specifically described below in combination with embodiments. It should be understood that the following text is only used to describe one or several specific embodiments of the present application, and does not strictly limit the protection scope of the present application.

[0030] Embodiment one:

[0031] As shown in Figure 1 , a bio-enzyme antibacterial cloth includes cloth substrate and bio-enzyme, the cloth substrate includes non-woven fabric, spunlaced fabric, wiping cloth, hot air cloth, etc., the bio-enzyme can be selected from lysozyme, protease, etc., since the bio-enzyme is closely combined with the cloth substrate, the cloth substrate is endowed with high efficient and durable antibacterial ability on the basis of maintaining the original physical properties (such as strength, softness, air permeability) of the cloth substrate, can effectively kill common harmful bacteria such as Escherichia coli, Staphylococcus aureus, Candida albicans, etc., the comprehensive antibacterial rate is more than 95%, and the bio-enzyme can still maintain good antibacterial activity after multiple washing, the antibacterial property of the bio-enzyme is verified by adding a certain amount of bio-enzyme solution after culture, using round culture medium and inoculating Escherichia coli, Staphylococcus aureus and Candida albicans respectively, the experimental results are shown in Table 1 below.

[0032] Table 1 experimental results of antibacterial property

[0033]

[0034] From the above table, the bio-enzyme used in the present embodiment can effectively kill common harmful bacteria such as E. coli, Staphylococcus aureus, and Candida albicans, and the comprehensive antibacterial rate is more than 95%, and the antibacterial activity can still be maintained after multiple water washing.

[0035] In addition, the finished product of the antibacterial cloth needs to be tested for safety, which is as follows:

[0036] 1. Skin sensitization test

[0037] Select a number of animals (such as mice, rabbits, etc.), directly apply bio-enzyme solution, or use antibacterial cloth with bio-enzyme to wipe the body, and observe for redness or edema symptoms after a certain period of time. The experimental results are shown in Table 2 below.

[0038] Table 2 Skin sensitization test results

[0039]

[0040]

[0041] Among them, the representation of initial body weight and final body weight is mean ± SD;

[0042] 2. Cosmetics human skin patch test

[0043] Select 30 subjects, apply antibacterial cloth with bio-enzyme to the surface of the body (such as arms), and observe for redness or edema reactions. The test results are shown in Table 3 below.

[0044] Table 3 Cosmetics human skin patch test results

[0045]

[0046]

[0047] From the cosmetics human skin patch test, it can be seen that the present antibacterial cloth product has high safety for human body and almost causes no adverse reactions;

[0048] 3. Small dose toxicology experiment

[0049] Taking the bio-enzyme solution of the present embodiment as an example, a number of animals were selected for oral toxicity test, and the individual state was observed. The experimental results are shown in Table 4 below.

[0050] Table 4 Small dose toxicology experiment results

[0051]

[0052] As can be seen from Table 4 above, no animal died in the case of using the biological enzyme solution in a small dose, and the production of the bacteriostatic cloth can be safely put into production;

[0053] 4. Large-dose toxicology experiment

[0054] Taking the biological enzyme solution of the present embodiment as an example, a number of animals were selected for oral toxicity test, and the individual state was observed. The experimental results are shown in Table 5 below.

[0055] Table 5: Results of large-dose toxicology experiment

[0056]

[0057] As can be seen from Table 5 above, no animal died in the case of using the biological enzyme solution in a large dose, and the biological enzyme solution was shown to be non-toxic to the individual in the acute oral toxicity test and the acute inhalation toxicity test, respectively, indicating that the production of the bacteriostatic cloth can be safely put into production.

[0058] Example 2

[0059] In order to facilitate the standardized manufacture of the biological enzyme bacteriostatic cloth in Example 1 by those skilled in the art, the relevant production steps are given in the present embodiment.

[0060] As shown in Figure 2 , a preparation method of a biological enzyme bacteriostatic cloth, comprising the following steps:

[0061] Step 1, biological enzyme solution preparation: select biological enzymes with high bacteriostatic activity, such as lysozyme and protease, dissolve them in a buffer solution according to a certain proportion, add an appropriate amount of stabilizer and protective agent, and the stabilizer and protective agent can be, for example, glycerol and trehalose (for example, after mixing the stabilizer and protective agent, mix the mixture with the biological enzyme at a mass ratio of 1:1), to prepare a biological enzyme solution with a concentration of 0.5%, and fully stir to make it uniform for use;

[0062] Among them, the buffer solution can be selected from any one of Tris-HCI buffer solution, phosphate buffer solution (PBS), HEPES buffer solution or borate buffer solution;

[0063] Step 2, liquid treatment: the cloth substrate made of non-woven cloth, spunlace cloth, wiping cloth or hot air cloth, etc. is conducted by the transmission roller of the Kiss Lora liquid application equipment, the liquid application device parameters are adjusted, the equipment is turned on, the biological enzyme solution is uniformly applied to the surface of the cloth substrate, and the biological enzyme solution is uniformly distributed on the surface of the cloth substrate in the form of mist or fine stream by using the principle of pressure difference and capillary action. The liquid application amount is controlled at 1 g of biological enzyme per square meter of cloth substrate;

[0064] Wherein, the liquid pressure is controlled at 0.1 MPa, the flow rate of the biological enzyme solution is 5 L / h, and the temperature is maintained at 15℃;

[0065] Referring to Figure 3 , the Kiss Lora liquid feeding device comprises a conveyor belt, a rack, a cloth guide roller 1, a biological enzyme solution storage tank 2, a conveying pipeline 3, a spray head 4, a liquid feeding tank 5, a liquid feeding roller 6, a liquid transfer roller 7, a water pressing roller 8, an Omega drying air box 9, and a winding roller 10. The cloth guide roller 1, the liquid feeding roller 6, the liquid transfer roller 7, the water pressing roller 8, and the winding roller 10 are all rotatably installed inside the rack. The pipeline 3, the spray head 4, the liquid feeding tank 5, and the Omega drying air box 9 are all installed inside the rack through a steel structure.

[0066] In operation, the cloth substrate is sequentially wound around the cloth guide roller 1, the liquid transfer roller 7, the water pressing roller 8, and the winding roller 10. The biological enzyme solution in the biological enzyme solution storage tank 2 is intermittently pumped out by a liquid pump, sprayed along the conveying pipeline 3 and the spray head 4 to the liquid feeding roller 6, and the excess biological enzyme solution falls into the liquid feeding tank 5. Since the liquid feeding roller 6 is in contact with the liquid transfer roller 7, the biological enzyme solution adheres to the surface of the liquid transfer roller 7. Thus, the biological enzyme solution is absorbed by the cloth substrate between the liquid transfer roller 7 and the water pressing roller 8, and passes through the Omega drying air box 9 upstream of the winding roller 10 for drying treatment in step three.

[0067] Step three, drying treatment: the cloth substrate after liquid feeding is directly dried under the set temperature and humidity conditions. The drying time is 10 s, and the drying temperature is set to 40℃. Until the cloth substrate is completely dried, the biological enzyme is firmly attached to the cloth substrate, and the antibacterial cloth is prepared. Finally, the winding roller 10 is used for winding and collecting.

[0068] A plurality of hot air outlets and circulating air ducts are arranged in the drying area to uniformly blow the hot air on the surface of the cloth substrate. A humidity sensor is used to monitor the drying environment humidity in real time, and the monitoring result is sent to the upper computer in real time.

[0069] Step four, quality detection: the finished antibacterial cloth is subjected to multiple detections, and samples are selected for appearance detection, physical performance detection, and antibacterial performance detection, as follows:

[0070] The appearance detection is mainly performed by visual inspection to observe whether the cloth surface is uniform and whether there are stains.

[0071] The physical performance detection includes longitudinal strip sample tensile strength, longitudinal elongation, transverse strip sample tensile strength, transverse elongation, water absorption multiple, water absorption amount, water retention capacity, and 5ML penetration time.

[0072] The antibacterial performance detection is mainly performed by the antibacterial experiment in Example 1 to ensure that the product quality meets the requirements. The detection experimental results are shown in Table 6.

[0073] Table 6 Quality detection experiment results

[0074]

[0075] Example Three:

[0076] A preparation method of a biological enzyme bacteriostatic cloth, and example two are basically same, its difference lies in:

[0077] Step one: preparation of a concentration of 2% biological enzyme solution;

[0078] Step two, liquid treatment: the flow of biological enzyme solution is 5L / h or 20L / h;

[0079] Step four, quality detection: the detection experiment results are as follows in table 7.

[0080] Table 7 Quality detection experiment results

[0081]

[0082]

[0083] Example Four:

[0084] A preparation method of a biological enzyme bacteriostatic cloth, and example three are basically same, its difference lies in:

[0085] Step two, liquid treatment: the liquid pressure is controlled at 0.1MPa;

[0086] Step four, quality detection: the detection experiment results are as follows in table 8.

[0087] Table 8 Quality detection experiment results

[0088]

[0089] Example Five:

[0090] A preparation method of a biological enzyme bacteriostatic cloth, and example two are basically same, its difference lies in:

[0091] Step two, liquid treatment: the liquid pressure is controlled at 0.5MPa, and the flow of biological enzyme solution is 20L / h; Step four, quality detection: the detection experiment results are as follows in table 9. Table 9 Quality detection experiment results

[0092]

[0093] Example Six:

[0094] A preparation method of a biological enzyme bacteriostatic cloth, and example three are basically same, its difference lies in:

[0095] Step two, liquid treatment: the liquid pressure is controlled at 0.5 MPa, and the flow rate of the biological enzyme solution is 20 L / h; Step four, quality detection: the detection results are shown in Table 10.

[0096] Table 10 Quality detection results

[0097]

[0098]

[0099] Example seven:

[0100] A method for preparing a biological enzyme bacteriostatic cloth, which is basically the same as that of Example two, with the difference being that:

[0101] Step two, liquid treatment: the temperature is kept at 35℃, and the liquid pressure is controlled at 0.1 MPa or 0.5 MPa; Step four, quality detection: the detection results are shown in Table 11.

[0102] Table 11 Quality detection results

[0103]

[0104] Example eight:

[0105] A method for preparing a biological enzyme bacteriostatic cloth, which is basically the same as that of Example three, with the difference being that:

[0106] Step two, liquid treatment: the temperature is kept at 15℃, and the liquid pressure is controlled at 0.1 MPa or 0.5 MPa; Step three, drying treatment: the drying time is 10 s, and the drying temperature is set to 40℃ or 85℃;

[0107] Step four, quality detection: the detection results are shown in Table 12.

[0108] Table 12 Quality detection results

[0109]

[0110]

[0111] Example nine:

[0112] A method for preparing a biological enzyme bacteriostatic cloth, which is basically the same as that of Example two, with the difference being that:

[0113] Step two, liquid treatment: the temperature is kept at 35℃, and the liquid pressure is controlled at 0.1 MPa or 0.5 MPa; Step three, drying treatment: the drying time is 30 s, and the drying temperature is set to 40℃ or 85℃;

[0114] Step four, quality detection: the detection results are shown in Table 13.

[0115] Table 13 quality detection results

[0116]

[0117]

[0118] In summary, by comparing the quality detection results of each of the experiments of Examples 2 to 9, it can be seen that the best comprehensive antibacterial performance of the finished antibacterial cloth is obtained when the following conditions are met: 2% of the biological enzyme solution is used, the liquid feeding pressure is controlled at 0.1 MPa, the flow rate of the biological enzyme solution is controlled at 5 L / h, the temperature is maintained at 35°C, and the subsequent drying time is 10s-30s.

[0119] The above description is only optional embodiments of the present application. It should be noted that, for those skilled in the art, without departing from the principles of the present application, a number of improvements and attempts can be made, and these improvements and attempts should also be considered as the protection scope of the present application. The structures, devices and operation methods not specifically described and explained in the present application, such as without special description and limitation, are implemented according to the conventional means in the art.

Claims

1. A bio-enzyme antibacterial cloth, characterized by: The invention comprises a cloth substrate and a biological enzyme, wherein the cloth substrate comprises materials such as non-woven fabric, spunlace fabric, wiping cloth or hot air cloth.

2. The bio-enzyme antibacterial cloth according to claim 1, characterized in that: The biological enzyme includes one or more of lysozyme or protease, and the loading amount of the biological enzyme on the cloth substrate is 1g / m 2 ~5g / m 2 .

3. A method for preparing a bio-enzyme antibacterial cloth, applied to a bio-enzyme antibacterial cloth according to any one of claims 1-2, characterized in that: The following steps are involved: Step 1: Prepare the enzyme solution: select the enzyme, dissolve it in a buffer solution, add a certain amount of stabilizer and protective agent to prepare the enzyme solution, and stir it evenly for later use; Step 2: Liquid application: Place the fabric substrate on the Kiss Lora liquid application device, set the parameters, and start the Kiss Lora liquid application device to evenly apply the enzyme solution to the surface of the fabric substrate; Step 3: Drying: The cloth substrate after being coated with the liquid is directly dried under the set temperature and humidity conditions until the cloth substrate is dry and the biological enzyme is firmly attached to the cloth substrate to make an antibacterial cloth, which is then rolled up and collected; Step 4: Quality inspection: Conduct multiple tests on the finished antibacterial cloth, select samples, and conduct appearance inspection, physical property inspection and antibacterial performance inspection respectively.

4. The bio-enzyme antibacterial cloth and preparation method thereof according to claim 3, characterized in that: The concentration of the biological enzyme solution in step 1 is 0.5% to 2%.

5. The bio-enzyme antibacterial cloth and preparation method thereof according to claim 3, characterized in that: The upper liquid pressure in step 2 is set to 0.1 MPa to 0.5 MPa, the flow rate of the enzyme solution is set to 5 L / h to 20 L / h, and the temperature is set to 15° C. to 35° C.

6. The bio-enzyme antibacterial cloth and preparation method thereof according to claim 3, characterized in that: The KissLora liquid application equipment comprises a conveyor belt, a frame, a cloth guide roller (1), a biological enzyme solution storage tank (2), a conveying pipeline (3), a nozzle (4), an upper liquid tank (5), a liquid application roller (6), a liquid application transfer roller (7), a water pressure roller (8), an Omega drying bellows (9), and a winding roller (10); The cloth guide roller (1), the liquid application roller (6), the liquid application transfer roller (7), the water pressure roller (8) and the winding roller (10) are all rotatably mounted inside the frame, and the pipeline (3), the nozzle (4), the liquid application tank (5) and the Omega drying bellows (9) are all fixed inside the frame.

7. The bio-enzyme antibacterial cloth and preparation method thereof according to claim 3, characterized in that: During the drying process in step three, multiple hot air outlets and circulating air ducts are set in the drying area to allow the hot air to blow evenly on the surface of the cloth substrate, and a humidity sensor is used to monitor the humidity of the drying environment in real time.

8. The bio-enzyme antibacterial cloth and preparation method thereof according to claim 3, characterized in that: The drying time of step 3 is set to 10s to 30s, and the drying temperature is set to 40°C to 85°C.

9. The bio-enzyme antibacterial cloth and preparation method thereof according to claim 3, characterized in that: The appearance inspection is carried out by online inspection to observe whether the cloth surface is uniform and whether there are stains or foreign objects.

10. The bio-enzyme antibacterial cloth and preparation method thereof according to claim 3, characterized in that: The physical property tests include longitudinal strip tensile strength, longitudinal elongation, transverse strip tensile strength, transverse elongation, water absorption multiple, water absorption capacity, water retention capacity and 5ML penetration time.

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