19 results about "Bacterial lysis" patented technology

The present application relates to the technical field of food safety, and particularly relates to a low-power water bath ultrasonic foodborne pathogenic bacteria lysis method and application thereof. The present application uses a low-power water bath ultrasonic machine, adds ABS small balls with a size of about 1 mm in a solution sample, uses ultrasonic to cause high-frequency vibration of the ABS small balls, and the local high sound pressure field and high-density cavitation field generated by the vibration can rapidly lyse bacteria. Meanwhile, the movement of the small balls can accelerate the rapid material exchange of the solution at different positions in the tube, so that 1 mL of the to-be-tested bacterial sample can be lysed within 3 minutes. The method provided by the present application has high lysis efficiency and short time consumption. When applied to foodborne pathogenic bacteria detection, nucleic acid purification and enrichment of the lysis liquid are not needed, the time and labor cost required for nucleic acid detection can be greatly shortened, and a new method of efficient, economical and practical bacterial lysis and nucleic acid extraction is provided for the field of food safety detection.

The integrated bacterial gene extraction and detection device comprises a stirring module, a bearing table, an illumination module, a temperature control module and a magnetic separation module, a bearing hole communicated with the interior of the bearing table is formed in the bearing table, and the stirring module is mounted at the top end of the bearing table on one side of the bearing hole; the stirring module is arranged to be capable of stirring a sample in the test tube, the temperature control module is arranged in the bearing table and can control the temperature of the sample in the test tube, and the illumination module is arranged in the bearing table, located below the temperature control module and used for providing a visible light source for the test tube; the magnetic separation module is installed on one side wall of the bearing table and can provide or not provide magnetic attraction force for the test tube, bacteria lysis, nucleic acid extraction, isothermal amplification and fluorescence image detection are integrated, and full-process detection from a sample to a result can be completed outdoors or on site under the condition that an external large instrument is not needed.

TITLE: MIXTURE OF BACTERIA, LYSATE, AND ISOLATED METABOLITES FROM BUHTAN'S CURCUMA LONGA TO INCREASE YIELD AND ANTI-INFLAMMATORY PROPERTIES IN AGRICULTURAL PRODUCTION. The present invention relates to a plant treatment composition characterized in that it contains a mixture of live bacteria, bacterial lysate, and metabolites from the rhizomes of Curcuma longa. Spraying or applying said composition to plants increases plant fertility and enhances the anti-inflammatory properties of cereals, fruits, and vegetables.

The present invention relates to orally administered postbiotic compositions, and discloses orally administered postbiotic compositions used for the treatment of solid tumors in patients, particularly colon cancer and / or lung cancer. The compositions comprise lysates of probiotic microorganisms belonging to the genera Bacillus, Bifidobacterium, Lactobacillus, Saccharomyces, and Streptococcus.

The present application discloses an oral metastatic composition for use in the treatment of solid tumors in a patient, in particular colon and / or lung cancer, comprising lysates of probiotic microorganisms derived from the genus Bacillus, Bifidobacterium, Lactobacillus, Saccharomyces and Streptococcus.

The application provides a nucleic acid extraction chip, a bacterial micro-fluidic integrated detection platform and a detection method. The nucleic acid extraction chip comprises a shunt layer, a filter layer and a flow aggregation layer which are closely attached in sequence; a sample inlet of the shunt layer is connected with a plurality of shunt ports through a plurality of first micro-channels; the filter layer comprises filter paper for enriching nucleic acid; a sample outlet of the flow aggregation layer is connected with a plurality of flow aggregation ports through a plurality of second micro-channels, and the number and distribution of the flow aggregation ports correspond to those of the shunt ports; the filter paper is arranged between the flow aggregation ports and the shunt ports. The detection platform comprises a bacterial lysis unit and a nucleic acid amplification reaction unit; the bacterial lysis unit comprises the nucleic acid extraction chip and a first temperature control module. The nucleic acid extraction chip and the detection platform have less demand for samples and reagents, shorter time consumption, reduced infection risk of detection personnel and improved extraction efficiency. The isothermal amplification method is adopted, the sensitivity is high, the reagent is pre-frozen and embedded in the detection hole, and the operation process is simplified.

The invention relates to the technical field of biology, in particular to a bacterial nucleic acid extraction kit and application thereof.The bacterial nucleic acid extraction kit comprises a bacterial lysis solution, cellulose microspheres, eluent and a matched nucleic acid extraction device and can efficiently lyse cell walls of gram-negative bacteria and gram-positive bacteria and rapidly purify nucleic acid; the time consumed by the whole nucleic acid extraction link does not exceed 10 minutes, the downstream PCR detection sensitivity can reach 10 <-9 > ng / mu L, and the timeliness and reliability of nucleic acid extraction are remarkably improved.

The application belongs to the field of nanomaterials and biomedicine, and provides a X03 phage-cerium oxide nanoscale enzyme composite treatment platform based on chemical cross-linking of polyethylene glycol, a synergistic treatment platform integrating bactericidal and detoxification functions, namely X03@CeO2, is constructed by covalently coupling phage X03 targeting pseudomonas aeruginosa and CeO2 nanoscale enzyme with phosphatase activity, which solves the problem that antibiotic and phage therapy exacerbate systemic inflammatory storm due to massive release of lipopolysaccharide caused by bacterial lysis when killing multiple drug-resistant gram-negative bacteria. In vitro and in vivo experiments show that the complex can efficiently remove bacteria and biofilm, simultaneously degrade released LPS, significantly inhibit the production of inflammatory factors, active oxygen burst and TLR4 pathway activation, greatly improve the survival rate in a lethal sepsis mouse model and reverse multiple organ damage. The application is suitable for treating sepsis caused by drug-resistant gram-negative bacteria.

The invention discloses peptidoglycan hydrolase as well as a biological material and application thereof. The technical problem to be solved by the invention is how to inhibit bacteria, including bacteriostasis and sterilization. The invention discloses application of peptidoglycan hydrolase, and particularly discloses application of peptidoglycan hydrolase as well as pharmaceutical salt or derivative thereof. The application is any one of the following items: A1) application in preparation of bacterial lysis products; a2) is applied to preparation of peptidoglycan products from lysis bacteria; a3) application in preparation of a product for inhibiting bacterial growth; a4) in the preparation of a product for inhibiting the formation of a bacterial biofilm; a5) application in preparation of a product for enhancing the sensitivity of bacteria to antibiotics; and A6) application in preparation of a sterilization product combined with a cell membrane permeation accelerant. A7) application in preparation of a product combined with a cell membrane permeability accelerant to disintegrate a biofilm; and A8) application in preparation of sterilization products. The protein is any one of the following items: B1) a protein with an amino acid sequence being a sequence 2 or a sequence 4; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the peptidoglycan hydrolase in B1), has 80% or more of identity with the protein shown in B1) and has the same activity with the protein shown in B1); b3), a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The protein disclosed by the invention has an obvious antibacterial effect when being used for gram-negative bacteria, and can be used for industrial production.

This invention relates to the field of nonwoven fabric preparation technology, specifically to a biochemically modified antibacterial nonwoven fabric and its preparation method. This invention modifies nano-zinc oxide and nano-titanium dioxide using the silane coupling agent KH-570, improving the dispersibility of the nanoparticles. Under light or contact conditions, the nanoparticles release reactive oxygen species to disrupt bacterial cell membranes and inhibit their metabolism, thereby giving the antibacterial modified polypropylene masterbatch stable antibacterial properties. Furthermore, this invention stabilizes the loading through a polydopamine coating, thus stabilizing the antibacterial properties of lysozyme. Lysozyme specifically hydrolyzes the peptidoglycan layer of bacterial cell walls, leading to bacterial lysis. Thiourea dioxide, as a reducing agent, maintains lysozyme activity, preventing oxidative inactivation, while simultaneously inhibiting bacterial metabolic enzyme activity. Through the synergistic effect of inorganic and biological dual-effects, this invention can cover Gram-positive bacteria, Gram-negative bacteria, and fungi, thereby improving the antibacterial properties of the nonwoven fabric to a certain extent.

The invention relates to the technical field of biology, in particular to a lysis solution for rapidly extracting bacterial nucleic acid and a rapid nucleic acid extraction method based on the bacterial lysis solution and magnetic bead separation and enrichment. The lysate is prepared from 50m of Mtris (pH = 7.5), 20mM of EDTA (Ethylene Diamine Tetraacetic Acid), 0.7 M of NaCl, 1.5 M of guanidine isothiocyanate, 1M of guanidine hydrochloride, 0.2 percent of SDS (Sodium Dodecyl Sulfate), 1.5 percent of Tween-20, 1.5 percent of Triton X-100 and 1 percent of PEPPG F68. The extraction method comprises the steps of sample pretreatment and cracking, magnetic bead combination, magnetic bead washing, magnetic bead redissolution and magnetic bead separation, and can be used for treating Gram-positive bacterium and Gram-negative bacterium samples. Silicon hydroxyl magnetic beads with specific particle sizes are adopted, a 2mM NaOH solution is used for washing, and a Tris-HCl (pH = 7.9) solution is used for redissolving. The lysate can be used for preparing a reagent for rapidly extracting bacterial nucleic acid, the extraction method can be applied to bacterial detection or molecular biology experiments, the nucleic acid extraction time is effectively shortened to be within 10 minutes, and an efficient and reliable technical means is provided for related research and detection work.

When bacteria with different bacteriolysis targets are detected, it was necessary to lyse the individual bacteria separately with lysis solutions containing lysis enzymes suitable for each bacteriolysis target, resulting in a complicated and delayed bacteriolysis process. The purpose of this invention is to provide a new bacteriolysis method for lysing bacteria belonging to two or more species in a sample, making the bacteriolysis process more efficient and rapid. Provided is method for lysing a group of bacteria in the sample, comprising the step of lysing the group of bacteria in the sample in a mixture solution obtained by mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid, wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase, and wherein the mixture solution has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.5 to 8.5 mS / cm, whereby bacteriolysis of two or more species of bacteria becomes possible.

Provided is a new bacteriolysis method capable of bacteriolysing multiple species of bacteria and making bacteriolysis treatment more efficient and faster. A bacteriolysis method for lysing multispecies bacterial groups in a specimen, wherein bacteriolysis of multispecies bacterial groups is made possible by a step that reacts a specimen with a surfactant and lytic enzyme in a liquid and bacteriolyses the multispecies bacterial groups present in the specimen.

This disclosure relates to compositions and methods for inducing bacterial lysis using antirepressor proteins that are capable of inhibiting prophage-encoded repressor proteins. Provided herein are polynucleotides and vectors encoding the antirepressor proteins and methods of use thereof to lyse bacteria and to treat bacterial infections. In an embodiment, the antirepressor protein is derived from a phage-inducible chromosomal island (PICI), for example Sam protein from Staphylococcus aureus pathogenicity island (SaPI), and the bacterial infection is caused by S. aureus harbouring a prophage φNM1.

The application discloses an antibacterial dendritic polyester macromolecule, a preparation method and application thereof, and belongs to the biomedical field. The amino is grafted on the branch of the dendritic macromolecule to prepare an ideal antibacterial polyester macromolecule; the protonation of the amino contains a positive charge structure, which can effectively penetrate into the bacterial cell membrane structure to cause the antibacterial mechanism of the biological macromolecule modification, so that the grafting abundance of the macromolecule amino is improved, and the antibacterial effect of the polyester macromolecule is enhanced.

This invention discloses a method for preparing Fe3O4-based magnetic nanomaterials. The nanomaterials use iron(III) oxide (Fe3O4) as a core, and phenylenediamine and copper are co-modified onto their surface via a stirring method. On one hand, the phenylenediamine surface is rich in functional groups, which is beneficial for subsequent functionalization modification; its aniline-like structure can effectively enhance the photothermal effect of the nanomaterials. On the other hand, after modification with copper, the nanomaterials have a high affinity for bacteria, achieving rapid enrichment and efficient capture of pathogenic bacteria through electrostatic and metal coordination interactions. Furthermore, the photothermal effect generated by this magnetic nanomaterial under infrared light irradiation can synergistically enhance its enzyme-like activity, achieving highly efficient sterilization. Most importantly, this material can also efficiently remove harmful products generated after bacterial lysis, including peptidoglycan and lipopolysaccharide. This invention effectively solves the technical defects of traditional antibacterial materials, such as limited functionality and inability to remove toxic lysis products, demonstrating strong practicality and broad application prospects.

The invention relates to the technical field of microbiological analysis and detection, in particular to a rapid detection method for flora characteristics. The detection method disclosed by the invention is a method for rapidly detecting flora characteristics of a target sample based on rapid bacterial lysis and a high-throughput sequencing technology, bacteria can be fully lysed, preparation of a 16S library can be rapidly completed, and then the bacteria are accurately quantified through high-throughput sequencing; the copy number of the bacteria is corrected by adopting the 16S rDNA content of the total bacteria in the sample to be detected, so that the difference between the reads number of the bacteria and the copy number result caused by different sampling amounts can be eliminated, the sampling deviation is eliminated, the detection result is more accurate, the rapid detection of the bacterial flora characteristics in the target sample is realized, and the detection efficiency is improved. The method has a wide application prospect in the field of bacterial diversity analysis.

The invention belongs to the technical field of food safety detection, and relates to a method for simultaneously extracting and detecting nucleic acid of listeria monocytogenes and salmonella. The method comprises the following steps: cracking listeria monocytogenes and salmonella by using a bacterial lysis solution and releasing nucleic acid of listeria monocytogenes and salmonella; the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are extracted at the same time through the synthesized magnetic beads Fe3O4 and Al < 3 + >, and the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are detected at the same time through double RPA / RT-RPA and RPA-LFA test paper. The method has the characteristics of rapidness, high sensitivity and low equipment requirement, and has practical application value in on-site instant detection of food safety.

The invention discloses application of Phocaecola massiliensis in preparation of a product for treating and / or improving ulcerative colitis, and belongs to the technical field of biological medicine and microbiology. The application of the Phocaecola massiliensis strain from the human body in preparation of the product for treating and / or improving the ulcerative colitis is provided for the first time, and a new direction is provided for treating and / or improving the ulcerative colitis. According to the present invention, the Phocaeola massiliensis is firstly provided as the component of the product for treating and / or improving the ulcerative colitis, the Phocaeola massiliensis is used for preparing the pharmaceutical composition or the functional food in the form of the viable bacteria, the inactivated bacteria or the bacterial lysate, and the pharmaceutical composition comprises the effective dose of the bacterial strain and the pharmaceutically acceptable carrier; the product can slow down weight loss, reduce disease activity index score, increase colon length, reduce colon histopathologic score or reduce pro-inflammatory cytokine level.