32 results about "Bacterial lysis" patented technology

The invention discloses a nucleic acid extraction method. The method comprises the following steps: carrying out impurity removal treatment on a to-be-extracted biological sample containing nucleic acid by adopting first magnetic beads; carrying out adsorption treatment of adsorbing the nucleic acid by adopting a second magnetic bead; wherein the outer surface of the magnetic microsphere body of the first magnetic bead is provided with at least one polymer with a branched chain, and the branched chain contains a functional group. The first magnetic beads adsorb impurities after the bacterial liquid is cracked, so that a biological sample can meet the requirement of subsequent nucleic acid extraction without centrifugation, a manner of needing a centrifugation step in a traditional process is replaced, the method is more efficient and quicker, and the extracted nucleic acid is high in purity and good in quality. The invention further develops a nucleic acid extraction kit which can be suitable for operation of an automatic nucleic acid extraction instrument, and the requirements of automation and high throughput are met.

The invention relates to the technical field of non-woven fabric preparation, in particular to biochemical modified antibacterial non-woven fabric and a preparation method thereof.After nano-zinc oxide and nano-titanium dioxide are modified through a silane coupling agent KH-570, the dispersity of nano-particles can be improved, and the antibacterial property of the non-woven fabric is improved; under illumination or contact conditions, active oxygen is released to destroy bacterial cell membranes and inhibit metabolism of the bacterial cell membranes, so that the antibacterial modified polypropylene master batch has stable antibacterial property; loading is stabilized through a polydopamine coating, so that the antibacterial performance of the lysozyme is stabilized, the lysozyme can specifically hydrolyze a peptidoglycan layer of a bacterial cell wall to cause bacterial lysis, thiourea dioxide serving as a reducing agent can maintain the activity of the lysozyme, oxidative inactivation is avoided, meanwhile, the activity of bacterial metabolic enzymes is inhibited, and the antibacterial activity of the lysozyme is improved. Through the inorganic / biological double-effect synergistic effect, gram-positive bacteria, gram-negative bacteria and fungi can be covered, so that the antibacterial performance of the non-woven fabric is improved to a certain extent.

The present application relates to the technical field of food safety, and particularly relates to a low-power water bath ultrasonic foodborne pathogenic bacteria lysis method and application thereof. The present application uses a low-power water bath ultrasonic machine, adds ABS small balls with a size of about 1 mm in a solution sample, uses ultrasonic to cause high-frequency vibration of the ABS small balls, and the local high sound pressure field and high-density cavitation field generated by the vibration can rapidly lyse bacteria. Meanwhile, the movement of the small balls can accelerate the rapid material exchange of the solution at different positions in the tube, so that 1 mL of the to-be-tested bacterial sample can be lysed within 3 minutes. The method provided by the present application has high lysis efficiency and short time consumption. When applied to foodborne pathogenic bacteria detection, nucleic acid purification and enrichment of the lysis liquid are not needed, the time and labor cost required for nucleic acid detection can be greatly shortened, and a new method of efficient, economical and practical bacterial lysis and nucleic acid extraction is provided for the field of food safety detection.

The integrated bacterial gene extraction and detection device comprises a stirring module, a bearing table, an illumination module, a temperature control module and a magnetic separation module, a bearing hole communicated with the interior of the bearing table is formed in the bearing table, and the stirring module is mounted at the top end of the bearing table on one side of the bearing hole; the stirring module is arranged to be capable of stirring a sample in the test tube, the temperature control module is arranged in the bearing table and can control the temperature of the sample in the test tube, and the illumination module is arranged in the bearing table, located below the temperature control module and used for providing a visible light source for the test tube; the magnetic separation module is installed on one side wall of the bearing table and can provide or not provide magnetic attraction force for the test tube, bacteria lysis, nucleic acid extraction, isothermal amplification and fluorescence image detection are integrated, and full-process detection from a sample to a result can be completed outdoors or on site under the condition that an external large instrument is not needed.

The invention relates to a micro-fluidic chip based on light-cured liquid drops, which comprises an oil phase liquid inlet used for introducing an oil phase containing a photopolymerization initiator and a light-cured monomer; the water phase liquid inlet is used for introducing a water phase containing a test sample, a bacterial lysis reagent and an enzymatic reaction substrate; the liquid drop generation intersection is used for forming water-in-oil liquid drops; the tree-shaped dispersion structure comprises a multi-stage bifurcated micro-channel and an inlet dispersion assembly and is used for realizing preliminary dispersion of liquid drops; the incubation chamber is a flat plate cavity, an internal dispersion assembly is arranged in the incubation chamber, liquid drops are solidified and fixed through an oil phase, bacteria are cracked through a water phase, and a substrate is catalyzed to generate a fluorescent product. The invention also relates to a method for detecting escherichia coli by using the micro-fluidic chip. The water-in-oil liquid drops are cured in situ, the problem that the liquid drops are easy to crack and fuse in incubation is thoroughly solved, the spatial fixity of the cured liquid drops facilitates the positioning collection of fluorescence signals, the signal drift interference in the traditional flowing liquid drop detection is reduced, and the method is suitable for ddPCR technology.

The invention discloses a paper-based sensor device based on a deoxyribozyme probe and application, and belongs to the technical field of analysis and detection. The paper-based sensor based on the deoxyribozyme probe by using the fluorescence signal is constructed, complex treatment on a sample is not needed, only a bacterial lysis buffer needs to be added into the sample for lysis, the deoxyribozyme is enabled to cut the deoxyribozyme probe by the generated escherichia coli CIM specificity, so that a sequence fragment containing an FAM group modified on the deoxyribozyme probe falls off, and the detection result is accurate. A complementary sequence modified at a detection line is used for capturing, a fluorescence signal is read to realize the detection purpose, and the whole reaction process can be completed within 30 minutes. The method integrates the processes of bacterial lysis, reaction sampling, bacterial detection and the like, avoids the complex and time-consuming operation process of a traditional method, has the analysis performance of rapidness, simplicity, rapidness, low cost and the like, and has potential application value in analysis and detection of various complex liquid bacteria in the clinical medicine field.

TITLE: MIXTURE OF BACTERIA, LYSATE, AND ISOLATED METABOLITES FROM BUHTAN'S CURCUMA LONGA TO INCREASE YIELD AND ANTI-INFLAMMATORY PROPERTIES IN AGRICULTURAL PRODUCTION. The present invention relates to a plant treatment composition characterized in that it contains a mixture of live bacteria, bacterial lysate, and metabolites from the rhizomes of Curcuma longa. Spraying or applying said composition to plants increases plant fertility and enhances the anti-inflammatory properties of cereals, fruits, and vegetables.

The present invention relates to orally administered postbiotic compositions, and discloses orally administered postbiotic compositions used for the treatment of solid tumors in patients, particularly colon cancer and / or lung cancer. The compositions comprise lysates of probiotic microorganisms belonging to the genera Bacillus, Bifidobacterium, Lactobacillus, Saccharomyces, and Streptococcus.

The present application discloses an oral metastatic composition for use in the treatment of solid tumors in a patient, in particular colon and / or lung cancer, comprising lysates of probiotic microorganisms derived from the genus Bacillus, Bifidobacterium, Lactobacillus, Saccharomyces and Streptococcus.

The invention provides a kit for detecting shigella by a Marker method, and the Marker method represents fluorescence intensity corresponding to shigella with different concentrations and is a basis for establishing a Marker graph; the kit comprises a Marker graph, a magnetic aptamer, a detection aptamer, a bacterial lysis solution, an enzyme digestion solution, a binding solution, a washing solution and a magnetic frame. The detection method comprises the following steps: the kit is used for splitting the shigella through a bacterial lysis solution to release DNA, the DNA is cut through an enzyme digestion solution to obtain a fragment rich in an ipaH gene, the magnetic aptamer and the detection aptamer are specifically combined with the ipaH fragment under the action of a binding solution to form a compound, the compound is washed with washing liquid, the fluorescence intensity is detected and compared with a Marker diagram, and quantitative detection of the shigella is realized. The method does not need to treat a sample in advance, shortens the detection time, and has strong specificity and high sensitivity.

The application provides a nucleic acid extraction chip, a bacterial micro-fluidic integrated detection platform and a detection method. The nucleic acid extraction chip comprises a shunt layer, a filter layer and a flow aggregation layer which are closely attached in sequence; a sample inlet of the shunt layer is connected with a plurality of shunt ports through a plurality of first micro-channels; the filter layer comprises filter paper for enriching nucleic acid; a sample outlet of the flow aggregation layer is connected with a plurality of flow aggregation ports through a plurality of second micro-channels, and the number and distribution of the flow aggregation ports correspond to those of the shunt ports; the filter paper is arranged between the flow aggregation ports and the shunt ports. The detection platform comprises a bacterial lysis unit and a nucleic acid amplification reaction unit; the bacterial lysis unit comprises the nucleic acid extraction chip and a first temperature control module. The nucleic acid extraction chip and the detection platform have less demand for samples and reagents, shorter time consumption, reduced infection risk of detection personnel and improved extraction efficiency. The isothermal amplification method is adopted, the sensitivity is high, the reagent is pre-frozen and embedded in the detection hole, and the operation process is simplified.

The invention relates to the technical field of biology, in particular to a bacterial nucleic acid extraction kit and application thereof.The bacterial nucleic acid extraction kit comprises a bacterial lysis solution, cellulose microspheres, eluent and a matched nucleic acid extraction device and can efficiently lyse cell walls of gram-negative bacteria and gram-positive bacteria and rapidly purify nucleic acid; the time consumed by the whole nucleic acid extraction link does not exceed 10 minutes, the downstream PCR detection sensitivity can reach 10 <-9 > ng / mu L, and the timeliness and reliability of nucleic acid extraction are remarkably improved.

The application belongs to the field of nanomaterials and biomedicine, and provides a X03 phage-cerium oxide nanoscale enzyme composite treatment platform based on chemical cross-linking of polyethylene glycol, a synergistic treatment platform integrating bactericidal and detoxification functions, namely X03@CeO2, is constructed by covalently coupling phage X03 targeting pseudomonas aeruginosa and CeO2 nanoscale enzyme with phosphatase activity, which solves the problem that antibiotic and phage therapy exacerbate systemic inflammatory storm due to massive release of lipopolysaccharide caused by bacterial lysis when killing multiple drug-resistant gram-negative bacteria. In vitro and in vivo experiments show that the complex can efficiently remove bacteria and biofilm, simultaneously degrade released LPS, significantly inhibit the production of inflammatory factors, active oxygen burst and TLR4 pathway activation, greatly improve the survival rate in a lethal sepsis mouse model and reverse multiple organ damage. The application is suitable for treating sepsis caused by drug-resistant gram-negative bacteria.

The invention discloses peptidoglycan hydrolase as well as a biological material and application thereof. The technical problem to be solved by the invention is how to inhibit bacteria, including bacteriostasis and sterilization. The invention discloses application of peptidoglycan hydrolase, and particularly discloses application of peptidoglycan hydrolase as well as pharmaceutical salt or derivative thereof. The application is any one of the following items: A1) application in preparation of bacterial lysis products; a2) is applied to preparation of peptidoglycan products from lysis bacteria; a3) application in preparation of a product for inhibiting bacterial growth; a4) in the preparation of a product for inhibiting the formation of a bacterial biofilm; a5) application in preparation of a product for enhancing the sensitivity of bacteria to antibiotics; and A6) application in preparation of a sterilization product combined with a cell membrane permeation accelerant. A7) application in preparation of a product combined with a cell membrane permeability accelerant to disintegrate a biofilm; and A8) application in preparation of sterilization products. The protein is any one of the following items: B1) a protein with an amino acid sequence being a sequence 2 or a sequence 4; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the peptidoglycan hydrolase in B1), has 80% or more of identity with the protein shown in B1) and has the same activity with the protein shown in B1); b3), a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The protein disclosed by the invention has an obvious antibacterial effect when being used for gram-negative bacteria, and can be used for industrial production.

This invention relates to the field of nonwoven fabric preparation technology, specifically to a biochemically modified antibacterial nonwoven fabric and its preparation method. This invention modifies nano-zinc oxide and nano-titanium dioxide using the silane coupling agent KH-570, improving the dispersibility of the nanoparticles. Under light or contact conditions, the nanoparticles release reactive oxygen species to disrupt bacterial cell membranes and inhibit their metabolism, thereby giving the antibacterial modified polypropylene masterbatch stable antibacterial properties. Furthermore, this invention stabilizes the loading through a polydopamine coating, thus stabilizing the antibacterial properties of lysozyme. Lysozyme specifically hydrolyzes the peptidoglycan layer of bacterial cell walls, leading to bacterial lysis. Thiourea dioxide, as a reducing agent, maintains lysozyme activity, preventing oxidative inactivation, while simultaneously inhibiting bacterial metabolic enzyme activity. Through the synergistic effect of inorganic and biological dual-effects, this invention can cover Gram-positive bacteria, Gram-negative bacteria, and fungi, thereby improving the antibacterial properties of the nonwoven fabric to a certain extent.

The invention discloses a recombinant mussel mucoprotein hyperbaric oxygen modification method which comprises the following steps: S1, optimizing a mussel mucoprotein gene sequence, cloning the optimized mussel mucoprotein gene sequence into an expression vector, and constructing a recombinant plasmid; s2, transforming the recombinant plasmids into competent cells, and culturing to obtain engineering bacteria; s3, culturing and fermenting the engineering bacteria, centrifugally collecting thalli, adding a bacteria breaking solution to break the thalli, purifying and collecting eluent, and dialyzing to obtain dialysate containing non-viscous recombinant mussel mucin; and S4, continuously introducing pure oxygen into the dialysate containing the non-viscous recombinant mussel mucoprotein, and adding a reinforcing agent for reaction to obtain the viscous recombinant mussel mucoprotein. The invention relates to a mussel mucoprotein modification method based on combination of a solid-phase catalyst physical separation technology and high-pressure oxygen, which realizes a green process without exogenous enzymes / chemical additives. The purity of the obtained product is greater than or equal to 98.9%, the protein recovery rate is greater than or equal to 95%, the adhesion strength and the DOPA content are relatively high, and a high-purity and low-cost mass production solution is provided for the fields of medical adhesives and the like.

The present invention relates to a method for determining the methicillin resistance properties of a strain of Staphylococcus aureus present in a biological sample, comprising the following steps: a) incubation of the biological sample containing said strain of Staphylococcus aureus for at least 15 minutes, in the presence of a beta-lactam antibiotic selected from the following group: cefoxitin and 6-APA (6-aminopenicillanic acid), b) isolation of the bacteria present in said biological sample, c) lysis of the bacteria and hydrolysis of the bacterial proteins, in order to obtain a mixture of peptides, d) analysis of this mixture of peptides by targeted mass spectrometry, the detection of at least one peptide from the PBP2a protein (SEQ ID NO. 1) or PBP2c protein (SEQ ID NO. 2) during this analysis step being indicative of the methicillin resistance of the strain of Staphylococcus aureus present in said biological sample.

The invention relates to the technical field of biology, in particular to a lysis solution for rapidly extracting bacterial nucleic acid and a rapid nucleic acid extraction method based on the bacterial lysis solution and magnetic bead separation and enrichment. The lysate is prepared from 50m of Mtris (pH = 7.5), 20mM of EDTA (Ethylene Diamine Tetraacetic Acid), 0.7 M of NaCl, 1.5 M of guanidine isothiocyanate, 1M of guanidine hydrochloride, 0.2 percent of SDS (Sodium Dodecyl Sulfate), 1.5 percent of Tween-20, 1.5 percent of Triton X-100 and 1 percent of PEPPG F68. The extraction method comprises the steps of sample pretreatment and cracking, magnetic bead combination, magnetic bead washing, magnetic bead redissolution and magnetic bead separation, and can be used for treating Gram-positive bacterium and Gram-negative bacterium samples. Silicon hydroxyl magnetic beads with specific particle sizes are adopted, a 2mM NaOH solution is used for washing, and a Tris-HCl (pH = 7.9) solution is used for redissolving. The lysate can be used for preparing a reagent for rapidly extracting bacterial nucleic acid, the extraction method can be applied to bacterial detection or molecular biology experiments, the nucleic acid extraction time is effectively shortened to be within 10 minutes, and an efficient and reliable technical means is provided for related research and detection work.

The invention provides a lyase general formula, and aiming at the general formula, the activity and the function of the lyase can be further screened. 24 lyases are preliminarily screened out, more specifically, the characteristics of 9 lyases are explored, and the 9 lyases have efficient bactericidal activity and can split various staphylococcus, streptococcus and / or enterococcus; the compound has good environmental resistance and stability, is stable in activity in solutions with different temperatures, different NaCl and different pH values, and is not easy to inactivate in immune serum; and local infection and systemic infection can be effectively treated. The lyase and variants thereof are expected to become antibacterial agents for removing bacteria, preventing bacterial infection or treating bacterial infection (staphylococcus, streptococcus and / or enterococcus bacteria).

When bacteria with different bacteriolysis targets are detected, it was necessary to lyse the individual bacteria separately with lysis solutions containing lysis enzymes suitable for each bacteriolysis target, resulting in a complicated and delayed bacteriolysis process. The purpose of this invention is to provide a new bacteriolysis method for lysing bacteria belonging to two or more species in a sample, making the bacteriolysis process more efficient and rapid. Provided is method for lysing a group of bacteria in the sample, comprising the step of lysing the group of bacteria in the sample in a mixture solution obtained by mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid, wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase, and wherein the mixture solution has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.5 to 8.5 mS / cm, whereby bacteriolysis of two or more species of bacteria becomes possible.

The invention provides a preparation method of lactobacillus paracasei metagen with anti-inflammatory activity, which comprises the following steps: activating lactobacillus paracasei, and suspending in an MRS culture medium to obtain lactobacillus paracasei bacterial liquid with the concentration of 106 CFU / mL; the preparation method comprises the following steps: carrying out low-temperature gradient inactivation to obtain an inactivated bacterial liquid, centrifuging, cleaning a precipitate with a phosphate buffer solution to obtain dead thalli, suspending with normal saline, crushing with an ultrasonic cell crusher, centrifuging the crushed dead thalli suspension, retaining the precipitate, namely a bacterial lysate, resuspending with an antibiotic-free DMEM (Dulbecco Modified Eagle Medium), and drying to obtain the antibiotic-free bacterial lysate. The lactobacillus paracasei metabiotics with the anti-inflammatory activity are obtained. The invention also provides an application for inhibiting the expression of a proinflammatory factor IL-1beta, promoting the expression of an anti-inflammatory factor IL-10 inflammatory factor and inhibiting the expression of the Myd88 gene. The lactobacillus paracasei metagen disclosed by the invention has relatively high anti-inflammatory activity and can be used for preparing fermented milk with a long shelf life.

The invention discloses a one-tube bacterial detection method and system based on bacterial lysis, RPA (recombinase polymerase amplification) and CRISPR-Cas12b (clustered regularly interspaced short palindromic repeats), and a rapid and portable detection scheme is constructed by organically combining bacterial lysis, RPA and CRISPR-Cas12b technologies. A physical separation technology is creatively adopted, RPA and CRISPR-Cas12b at different reaction temperatures are integrated in the same reaction tube, two-step reaction can be ensured to be independently carried out, and the condition that CRISPR consumes an RPA amplification substrate in advance is avoided, so that the detection sensitivity is remarkably improved, and meanwhile, the aerosol pollution risk caused by uncovering operation is eliminated. The detection scheme can be realized by combining a simple fluorescent reading device, has the characteristics of high specificity, high sensitivity and convenience in operation, is suitable for scenes such as home detection and on-site rapid screening, and shows a wide application prospect.

Provided is a new bacteriolysis method capable of bacteriolysing multiple species of bacteria and making bacteriolysis treatment more efficient and faster. A bacteriolysis method for lysing multispecies bacterial groups in a specimen, wherein bacteriolysis of multispecies bacterial groups is made possible by a step that reacts a specimen with a surfactant and lytic enzyme in a liquid and bacteriolyses the multispecies bacterial groups present in the specimen.

A method for maintaining skin corneum layer completeness, promoting hyaluronic acid production, improving skin cell mitochondrion activity and / or promoting skin cell proliferation in a subject in need thereof, includes administering to the subject a composition including bacterial lysate of a H. trueperi TCI66207 strain. The Halobacillus trueperi TCI66207 strain is deposited in Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures under an accession number of DSM 33381.

This disclosure relates to compositions and methods for inducing bacterial lysis using antirepressor proteins that are capable of inhibiting prophage-encoded repressor proteins. Provided herein are polynucleotides and vectors encoding the antirepressor proteins and methods of use thereof to lyse bacteria and to treat bacterial infections. In an embodiment, the antirepressor protein is derived from a phage-inducible chromosomal island (PICI), for example Sam protein from Staphylococcus aureus pathogenicity island (SaPI), and the bacterial infection is caused by S. aureus harbouring a prophage φNM1.

The application discloses an antibacterial dendritic polyester macromolecule, a preparation method and application thereof, and belongs to the biomedical field. The amino is grafted on the branch of the dendritic macromolecule to prepare an ideal antibacterial polyester macromolecule; the protonation of the amino contains a positive charge structure, which can effectively penetrate into the bacterial cell membrane structure to cause the antibacterial mechanism of the biological macromolecule modification, so that the grafting abundance of the macromolecule amino is improved, and the antibacterial effect of the polyester macromolecule is enhanced.

This invention discloses a method for preparing Fe3O4-based magnetic nanomaterials. The nanomaterials use iron(III) oxide (Fe3O4) as a core, and phenylenediamine and copper are co-modified onto their surface via a stirring method. On one hand, the phenylenediamine surface is rich in functional groups, which is beneficial for subsequent functionalization modification; its aniline-like structure can effectively enhance the photothermal effect of the nanomaterials. On the other hand, after modification with copper, the nanomaterials have a high affinity for bacteria, achieving rapid enrichment and efficient capture of pathogenic bacteria through electrostatic and metal coordination interactions. Furthermore, the photothermal effect generated by this magnetic nanomaterial under infrared light irradiation can synergistically enhance its enzyme-like activity, achieving highly efficient sterilization. Most importantly, this material can also efficiently remove harmful products generated after bacterial lysis, including peptidoglycan and lipopolysaccharide. This invention effectively solves the technical defects of traditional antibacterial materials, such as limited functionality and inability to remove toxic lysis products, demonstrating strong practicality and broad application prospects.

The invention relates to the technical field of microbiological analysis and detection, in particular to a rapid detection method for flora characteristics. The detection method disclosed by the invention is a method for rapidly detecting flora characteristics of a target sample based on rapid bacterial lysis and a high-throughput sequencing technology, bacteria can be fully lysed, preparation of a 16S library can be rapidly completed, and then the bacteria are accurately quantified through high-throughput sequencing; the copy number of the bacteria is corrected by adopting the 16S rDNA content of the total bacteria in the sample to be detected, so that the difference between the reads number of the bacteria and the copy number result caused by different sampling amounts can be eliminated, the sampling deviation is eliminated, the detection result is more accurate, the rapid detection of the bacterial flora characteristics in the target sample is realized, and the detection efficiency is improved. The method has a wide application prospect in the field of bacterial diversity analysis.

The invention belongs to the technical field of food safety detection, and relates to a method for simultaneously extracting and detecting nucleic acid of listeria monocytogenes and salmonella. The method comprises the following steps: cracking listeria monocytogenes and salmonella by using a bacterial lysis solution and releasing nucleic acid of listeria monocytogenes and salmonella; the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are extracted at the same time through the synthesized magnetic beads Fe3O4 and Al < 3 + >, and the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are detected at the same time through double RPA / RT-RPA and RPA-LFA test paper. The method has the characteristics of rapidness, high sensitivity and low equipment requirement, and has practical application value in on-site instant detection of food safety.

The invention relates to a micro-fluidic chip based on electric cracking and protein purification. A metal electrode layer is combined on a glass substrate; the bacterial lysis and protein purification detection module is connected with the glass substrate; the bacterial lysis and protein purification detection module is internally provided with a lysis cavity, an enzyme substrate cavity and a chromogenic reaction cavity which are communicated through a micro-channel at the bottom, the metal electrode layer comprises a silver paste interdigital electrode, and the silver paste interdigital electrode is aligned with the lysis cavity, is positioned below the lysis cavity and is in an exposed state in the lysis cavity; the silver paste interdigital electrode generates a strong electric field to realize efficient splitting decomposition of bacteria. The invention also relates to application of the micro-fluidic chip in biological sample analysis. According to the present invention, the electric splitting method and the microfluidic technology are integrated, the splitting and the accurate quantitative detection of the bacteria are achieved, and compared with the prior art, the method has the great advantages in the detection sensitivity, the process automation, the integration level and the like, and has the wide application prospect in the molecular diagnosis aspect.