39 results about "Pseudomonas nitroreducens" patented technology

The invention discloses Pseudomonas nitroreducens and application thereof, belonging to the field of environmental microorganism. The Pseudomonas nitroreducens CQ1 of the invention is derived from thesewage of oxidation ponds in the sewage treatment system of Beijing Yanshan Petrochemical Co., Ltd., and obtained by enriching, separating and purifying. The Pseudomonas nitroreducens CQ1 is culturedand collected in the China General Microbiological Culture Collection Center (hereinafter referred to as CGMCC) with the collection number being CGMCC No.3159. The invention further discloses the application of Pseudomonas nitroreducens in the biodegradation of quinoline or derivatives thereof. Quinoline or derivatives thereof with the concentration reaching 700mg / L can be degraded by Pseudomonasnitroreducens of the invention, and the degradability can reach 100% after treating for 72h. Moreover, the Pseudomonas nitroreducens CQ1 of the invention is sensitive to kanamycin sulfate, tetracycline and gentamicin and does not carry plasmids.

The Pseudomonas nitroreducens S1 is prepared by separating out from active sludge in sewage disposal tank of petrochemical plant, purifying and culturing. The preservative number is CGMCC No. 1488. It has excellent efficient degradable performance for benzothiazole and can be used to dispose waste water and waste gas containing benzothiazole, polluted water and soil.

The invention belongs to the field of microbial preparations, and in particular relates to a composite microbial preparation for treating domestic sewage and a preparation method thereof. The composite microbial preparation for treating the domestic sewage comprises rhodopseudomonas palustris ACCC10649, saccharomyces cerevisiae ACCC20251, lactobacillus acidophilus ACCC11073, bacillus subtilis ACCC10619, pseudomonas nitroreducens ACCC04184, acinetobacter lwoffii ACCC01091 and candida tropicalis ACCC20004. According to the composite microbial preparation for treating the domestic sewage and the preparation method thereof, which are provided by the invention, organic matters in the sewage are converted into simple inorganic matters through decomposition of microorganisms, so that the sewage is treated effectively.

A microbial process is provided for selective cleavage of only organic C-N bonds while leaving C-C bonds intact which may be used for reducing the nitrogen content of nitrogen-containing organic carbonaceous materials. Microorganisms of Pseudomonas ayucida have been found which have the ability of selective cleavage of organic C-N bonds. A particularly preferred microorganism is Pseudomonas ayucida strain ATCC No PTA-806. Other microorganisms useful in the cleavage of organic C-N bonds are Aneurinibacillus sp, Pseudomonas stutzeri, Yokenella sp. and Pseudomonas nitroreducens.

The invention discloses a method for preparing theanine by a biological process, which comprises the following steps: preparing cells by using Pseudomonas nitroreducens NTLC4.002 with the collection number of CCTCC NO:M2014254 as the initial strain, and preparing natural theanine by cell transformation. The novel theanine strain applicable to theanine production is separated and screened from the Chinese traditional fermentation product; and the glutaminase generated by the strain can be used for efficiently producing theanine by acting on glutamine and ethylamine under the conditions of pH 9-10 and 35-40 DEG C, wherein the conversion rate can reach more than 80%.

The invention provides a Pseudomonas nitroreducens. strain and application thereof. The strain is Pseudomonas nitroreducens. Pf2, and is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2021274. The strain can synchronously perform nitrification and denitrification by taking organic carbon as a carbon source under an aerobic condition, and is suitable for removing organic wastewater containing ammonia nitrogen or nitrate, the NH4 < + > -N removal rate can reach 92.7 percent, and the NO3 < - > -N removal rate can reach 100 percent; and the treatment process can be greatly simplified, the treatment efficiency is improved, and the operation and maintenance cost is reduced.

The invention provides pseudomonas nitroreducens 5-hydroxymethylfurfural oxidase and a preparation method thereof. Particularly, the DNA sequence of 5-hydroxymethylfurfural oxidase is cloned to plasmid, and recombinant plasmid is integrated into host bacteria to obtain a gene engineering strain capable of heterologous expression of the oxidase. The prepared 5-hydroxymethylfurfural oxidase subjected to heterologous expression of the strain can oxidize the 5-hydroxymethylfurfural oxidase and convert the 5-hydroxymethylfurfural oxidase into 2,5-furandicarboxylic acid, furan-2,5-dicarbaldehyde and5-formyl-2-furancarboxylic acid. The invention provides a technological base for preparing furan bulk chemicals by oxidizing 5-hydroxymethylfurfural through bioanalysis.

The invention discloses a method for preparing theanine by using a biological method. The method comprises the steps of bacteria preparation, cell transformation, decoloring, electrodialysis, preparation of theanine powder products or theanine crystal products, and the like. According to the invention, free pseudomonas nitroreducens is adopted for synthesizing theanine by catalysis, and by using a method for adding substrates in batches, the yield of theanine is improved, the cost is lowered, and a high-concentration reaction is realized. A technological enzyme reaction system does not contain culture elements such as a carbon source, a nitrogen source, and the like, so that compared with fermentation liquor, reaction liquid is simple in composition, therefore, the product purity can be greatly improved; and the method has the advantages of easiness for separating an enzyme source from the reaction liquid, simple post-processing, short production cycle, high yield, small environmental pollution, low cost, and the like.

The invention discloses a heterotrophic nitrification-aerobic denitrification pseudomonas bacterium agent as well as a preparation method and application thereof. The heterotrophic nitrification-aerobic denitrification pseudomonas bacterium agent is a pseudomonas sp. W12 strain and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation location is Institute of Microbiology, Chinese Science Academy, #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing; the registered preservation number is CGMCC No. 12460 and the preservation date is May 17, 2016. The heterotrophic nitrification-aerobic denitrification pseudomonas bacterium agent takes sweet potato wastewater as a main culture medium and the W12 strain is inoculated; air is introduced to culture the strain to obtain a pseudomonas W12 liquid bacterium agent. After a proper amount of the pseudomonas W12 liquid bacterium agent is added, the average removal rate of a membrane bioreactor onammonia nitrogen is remarkably improved, and the average removal rate on total nitrogen is more remarkably improved, so that the water quality of output water of the membrane bioreactor is improved.

The invention relates to the field of microorganisms and provides degradative bacteria capable of degrading allelochemicals in plant root exudates and an application of the degradative bacteria. The degradative bacteria are classified and named Achromobacter xylosoxidans and Pseudomonas nitroreducens, and strain preservation numbers are CGMCC NO.14517 and CGMCC NO.14518 respectively. The degradative bacteria for the allelochemicals can degrade the allelochemicals in multiple degraded plant root exudates.

The invention discloses diffusible signal factor (DSF) quorum sensing signal degradation genes and application thereof. The degradation genes are degradation genes dig1, dig2, dig3 and dig4 separatedfrom pseudomonas nitroreducens bacterial strains HS-18 and having a remarkable degradation effect on quorum sensing signal DSF family signals. The degradation genes are subjected to heterologous expression in nonpathogenic bacteria or biocontrol bacteria, and the degradation ability of the nonpathogenic bacteria or the biocontrol bacteria to DSFs can be remarkably improved; the degradation genes are subjected to heterologous expression in DSF family signal mediated pathogenic bacteria, expression of virulent factors regulated and controlled by the DSF family signals can be remarkably influenced, and the pathogenicity of the pathogenic bacteria to plant hosts is lowered; and thus the DSF quorum sensing signal degradation genes have important practical application value and application prospects in the aspect of biological prevention and treatment of the DSF family signal mediated pathogenic bacteria.

The invention discloses Pseudomonas (sp.) HC-1. The (sp.) HC-1is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 11924, and the preservation date of December 24, 2015. The Pseudomonas (sp.) HC-1 has high aerobic denitrifying property, and is applied to sewage biological nitrogen removal process, the total nitrogen removal rate reaches 88.2 percent, and the characteristics have great significance in the field of water treatment.

The invention discloses a pyrethroid insecticide degrading strain as well as a microbial inoculum and a degrading process thereof. The invention obtains pseudomonas nitroreducens CW-7 through screening and separation, the strain is preserved in Guangdong province microbial strain preservation center on June 17, 2020, and the preservation number is GDMCC No. 61063. The strain has broad spectrum fordegrading pyrethroid insecticides and can be used for repairing natural environments such as water bodies and soil polluted by the pyrethroid insecticides, and the residual quantity of the pyrethroidinsecticides in the water bodies and the soil can be reduced by more than 95% after directly applying the strain for 7 days; and the prepared excellent degrading bacterium agent is applied to bioremediation of environments such as water bodies and soil polluted by the pyrethroid insecticides, provides a new microbial resource for bioremediation of pesticide environmental pollution, enriches a germplasm resource library of pesticide degrading bacteria, and provides a theoretical basis and a practical basis for developing a new technology for treating pyrethroid insecticide pollution such as allethrin.

The invention relates to the field of cellulose degradation and discloses pseudomonas nitroreducens as well as a fermentation product and application thereof. The preservation number of the pseudomonas nitroreducens provided by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.13412. The invention further discloses a method for culturing the pseudomonas nitroreducens; the method comprises the following steps: inoculating the pseudomonas nitroreducens into a fermentation culture medium and fermenting. The invention further discloses a method for degrading cellulose and the method comprises the step of enabling a degradation agent to be in contact with a raw material containing the cellulose; the degradation agent is the pseudomonas nitroreducens and / or the fermentation product of the pseudomonas nitroreducens. Furthermore, the invention discloses application of the pseudomonas nitroreducens and / or the fermentation product of the pseudomonas nitroreducens to degradation of the cellulose. The pseudomonas nitroreducens disclosed by the invention can be used for effectively degrading the cellulose, the fermentation product has various enzyme activities and the enzyme activities are relatively high.

The invention discloses a method for preparing theanine by a biological process, which comprises the following steps: preparing cells by using Pseudomonas nitroreducens NTLC4.002 with the collection number of CCTCC NO:M2014254 as the initial strain, and preparing natural theanine by cell transformation. The novel theanine strain applicable to theanine production is separated and screened from the Chinese traditional fermentation product; and the glutaminase generated by the strain can be used for efficiently producing theanine by acting on glutamine and ethylamine under the conditions of pH 9-10 and 35-40 DEG C, wherein the conversion rate can reach more than 80%.

A biological method for removing organic polymers is provided. The method includes the cultivation of a bacterial strain Pseudomonas nitroreducens TX1. The deposit numbers are PTA-6168 at ATCC and BCRC910228 at Bioresources Collection and Research Center in Taiwan (Republic of China). A nitrogen source and inorganic salts are required for bacterial growth. Moreover, the method also provides the information of the supply of air and the water content in soils when this invention is applied to the removal of organic polymers in soils. In addition, the viability of cultivated bacteria is not affected by endogenous bacteria in soils and able to maintain the capacity of degrading organic polymers in the method. The present invention is able to remove the organic polymers in a wide range of amounts effectively and useful in the bioremediation of contamination from organic polymers in both soils and water.

The invention relates to the field of microorganisms, and discloses Pseudomonas nitroreducens, a microbial inoculum, application of the Pseudomonas nitroreducens and the microbial inoculum and a method for degrading acetaldehyde, and the preservation number of the Pseudomonas nitroreducens is CGMCC No.23134. According to the present invention, the Pseudomonas nitroreducens can survive and grow in acetaldehyde-containing pollutants, can maintain the acetaldehyde degradation ability in the presence of acrolein, and can coexist with formaldehyde degradation bacteria to achieve the good acetaldehyde degradation effect; and the invention provides an available strain for degrading acetaldehyde in acetaldehyde pollutants by a biological method, can realize in-situ pollutant degradation so as to reduce the cost of acetaldehyde degradation, has the advantages of simplicity and convenience in operation, less interference, less secondary pollution and the like, and is suitable for large-scale fermentation production and long-term use.

The invention discloses a method for producing L-theanine by using a fermentation method. The method comprises the following steps of: inoculating brevibacteriumflavum into a seed culture medium for expanding culture to obtain first seed liquid; inoculating nitro-reducing pseudomonas into the seed culture medium for expanding culture to obtain second seed liquid; inoculating the first seed liquidand the second seed liquid into a fermentation culture medium containing matcha powder for fermentation to obtain first fermentation liquid, adding ethylamine into the first fermentation liquid, and continuously fermenting and culturing to obtain second fermentation liquid; adding ethylamine into the second fermentation liquid to obtain third fermentation liquid. According to the invention, the glutamine generated by microbial fermentation is directly utilized through the combined fermentation culture of the brevibacteriumflavum and the nitro-reducing pseudomonas, so that the production process is simplified, and the production cost is reduced. According to the invention, the matcha powder is added into the fermentation culture medium, and the ethylamine is added in batches in the fermentation process, so that the fermentation time is shortened, the yield of the L-theanine is improved.

The invention discloses a tetracycline antibiotic degrading bacterium and its application in remediation of soil pollution. The degrading bacterium is Pseudomonas nitroreducens. Each single strain, which is obtained after pig manure samples undergo screening, domestication, isolation and purification with tetracycline antibiotic tolerant bacteria, is inoculated into an inorganic salt medium containing the only carbon source of tetracycline antibiotics for degradation experiments and screening to obtain the strain with the strongest ability of degrading tetracycline antibiotics in the inorganicsalt medium. According to the invention, immobilized microbial inoculum is safe, effective, harmless, environmentally-friendly, green and cheap in repairing soil tetracycline antibiotic oxytetracycline and tetracycline pollution without causing secondary pollution; meanwhile, the bioavailability of tetracycline antibiotics can be improved, and the efficiency of microbial degradation of tetracycline antibiotics is further enhanced.

The invention discloses an isoeugenol monooxygenase mutant and application thereof, and belongs to the field of bioengineering. The isoeugenol monooxygenase gene derived from Pseudomonas nitroreducens Jin1 is subjected to site-specific mutagenesis, so that the 83rd site of the coded amino acid sequence is mutated from lysine K to arginine R, the 95th site of the coded amino acid sequence is mutated from lysine K to arginine R, and the 273rd site of the coded amino acid sequence is mutated from leucine to phenylalanine F. The specific activity of the isoeugenol monooxygenase mutant K83R / K95R / L273F is 2.1 times that before mutation, and the half-life periods of the isoeugenol monooxygenase mutant K83R / K95R / L273F at 25 DEG C, 30 DEG C and 35 DEG C are respectively improved by 3.0 times, 12.0 times and 24.7 times. The isoeugenol monooxygenase mutant disclosed by the invention can catalyze propenyl on a benzene ring of a substrate isoeugenol to oxidize in a relatively wide temperature range under the action of oxygen so as to generate vanillin, can be widely applied to industries such as food, chemical engineering and pharmacy, and has a wide prospect.

The invention belongs to the technical field of molecular biological control, and the research finds that the pseudomonas nitroreducens strain HS-18 has good degradation activity on AHLs with C4-C14 chain length for detection, the degradation effect is efficient and remarkable, and a new biocontrol preparation is provided for biological control of AHLs mediated pathogenic bacteria, so that the species of the quorum quenching sterilization of the AHLs signal molecules are widened.

The invention belongs to the technical field of molecular biological prevention and treatment, and finds that an N-acyl homoserine lactone acyltransferase coding gene is cloned through a molecular biological technology on the basis that pseudomonas nitroreducens HS-18 can efficiently degrade AHLs signal molecules with the acyl chain length of C4-C14 in early-stage research. According to the invention, in early-stage research, on the basis that pseudomonas nitroreducens HS-18 can efficiently degrade AHLs signal molecules with C4-C14 acyl chains, an N-acyl homoserine lactone acyltransferase coding gene is cloned through a molecular biological technology. The gene has broad-spectrum and efficient quenching activity on AHLs with different side chain lengths and different side chain substituted band groups. By expressing the N-homoserine lactone quenching gene in AHLs pathogenic pathogenic bacteria, the motility of the pathogenic bacteria, biofilm formation and generation of virulence factors such as extracellular enzymes can be remarkably weakened, and the pathogenicity of the pathogenic bacteria to host plants is remarkably weakened.

The invention belongs to the technical field of molecular biological prevention and treatment, and finds that an N-acyl homoserine lactone acyltransferase coding gene is cloned through a molecular biological technology on the basis that pseudomonas nitroreducens HS-18 can efficiently degrade AHLs signal molecules with the acyl chain length of C4-C14 in early-stage research. The N-acyl homoserine lactone acyltransferase coding gene has broad-spectrum quenching activity on AHLs, and has a good degradation effect on N-acyl homoserine lactones with different carbon chain lengths and modified with different substituent groups. The previous research results show that most of the N-acyl homoserine lactone acyltransferase coding genes have good quenching activity on long-chain AHLs. The N-acyl homoserine lactone acyltransferase coding gene lays an important foundation for enriching N-acyl homoserine lactone acyltransferase coding gene resources.

The invention relates to the technical field of microorganisms, and particularly discloses a microbial agent suitable for corn planting, and application thereof. The microbial agent disclosed by the invention comprises at least one of the following strains: acinetobacter johnsonii, acinetobacter lwoffii, brevundimonas natans, burkholderia cepacia, microbacterium paraoxidans, pseudomonas aeruginosa, pseudomonas oryzae, pseudomonas ferrawiae, pseudomonas nitroreducens, pseudomonas formosana, rhodococcus ruber. serratia marcescens and neosphingosine resinophila. The microbial agent provided by the invention can promote absorption of beneficial mineral elements by corn, improves the problem of enrichment of heavy metals in soil by corn in an existing planting system, and reduces the heavy metal content of corn; moreover, compared with a conventional corn planting method, a planting method in the invention has the advantages that the corn germination speed and germination rate can be better increased, and the development condition of the root system of the corn in the seedling stage and the leaf surface development condition of the corn in the seedling stage can be improved.

The invention relates to the field of microorganisms, discloses chili endophytic bacteria highly producing ironophore and an application of the chili endophytic bacteria to production increase of chilies, and specifically provides the chili endophytic bacteria highly producing ironophore. The chili endophytic bacteria highly producing ironophore are pseudomonas nitroreducens PEB40, and the preservation number is CCTCC No: M2019683. The invention also provides a microbial inoculum. The microbial inoculum contains the chili endophytic bacteria highly producing ironophore. The invention also provides a method of increasing production of chilies. The method comprises the steps of enabling the chili endophytic bacteria highly producing ironophore and / or the microbial inoculum to be in contact with chili seedlings and / or chili plants. According to the strain provided by the invention, the microbial inoculum capable of promoting growth of chilies is prepared by a simple and quick operating method, so that the chilies treated by the microbial inoculum can be exuberant in vigor, and the strain is simple and convenient to use, low in cost and notable in effects, and besides, green, organic and pollution-free.

The invention discloses a pseudomonas nitroreducens and application thereof in degradation of DSF (diffusible signal factor) and / or DSF analogues. The pseudomonas nitroreducens disclosed by the invention is named as HS-18, and is preserved in China Center for Type Culture Collection on May 12, 2017 with a preservation number of CCTCC NO:M2017257. The strain has stable activity, can take DSF as thesole carbon source for growth, can completely degrade DSF within 24h, has a significant and rapid degradation effect on DSF, and has high application value in quorum quenching direction, black rot disease inhibition, and control of the harm of DSF mediation dependent pathogenic bacteria. The pseudomonas nitroreducens provided by the invention can reduce the pesticide abuse problem, and at the same time provides a new idea for biological control of the harm of phytopathogen.