11 results about "Pseudomonas nitroreducens" patented technology

The invention belongs to the technical field of microorganism application, and particularly relates to pseudomonas and application thereof in agricultural waste compost deodorization. The strain is Pseudomonas nitroreducens XYT03, and is preserved in the China General Microbiological Culture Collection Center, called CGMCC for short, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 14, 2025, and the preservation number is CGMCC NO.35224. The invention further discloses a preparation method of the Pseudomonas nitroreducens XYT03. The invention also provides an application of the strain in agricultural waste compost deodorization, and the strain can efficiently degrade ammonia gas, hydrogen sulfide and other malodorous substances generated in the composting process, and has the advantages of high deodorization efficiency, strong environmental adaptability, no secondary pollution and the like.

The invention discloses a pseudomonas nitroreducens MHY01 and an application of the pseudomonas nitroreducens MHY01 as a thifluzamide synergist. According to the invention, a strain capable of promoting rice to absorb thifluzamide is separated from healthy rice root soil, the strain is named as pseudomonas nitroreducens MHY01, and a composite flora capable of promoting pesticide absorption is further obtained. The strain and the composite flora can significantly reduce pesticide soil adsorption loss, reduce soil and water pollution risks, alleviate drug resistance development and promote agricultural sustainable development on the premise of ensuring the disease and pest control effect in practical application. And secondly, the strain is derived from healthy rice rhizosphere, is green and safe, has no obvious influence on rice growth, and ensures the quality safety and ecological safety of agricultural products. Therefore, the utilization efficiency of the pesticide is improved by developing rhizosphere microorganisms, and the problems that the utilization rate is low and the dosage is increased due to the fact that the pesticide is adsorbed by soil are fundamentally solved.

The present application belongs to the technical field of biological fermentation, and relates to a culture medium-free biological conversion method of citrus essential oil based on compound strains, comprising the following steps: 1, preparing wet bacteria bodies of Bacillus cereus GJ-02 and Pseudomonas nitroreducens GJ-01 respectively; 2, compounding the obtained two kinds of wet bacteria bodies; 3, suspending the compounded wet bacteria bodies in a buffer solution, and adding citrus essential oil to perform a culture medium-free biological conversion reaction; and 4, performing post-treatment on the obtained reaction liquid to recover a perfume product; the culture medium in the conversion stage is omitted, the process is simple, the cost is low, and there is no pollution, and the product synergism is realized through strain compounding, the obtained perfume has full and coordinated aroma, and is particularly suitable for tobacco flavoring, food and daily chemical perfume fields.

The application discloses a bacterial composition for promoting the growth and saponin accumulation of Panax notoginseng, which is composed of three strains, including Paenarthrobacter nicotinovorans SQ 15, Bacillus velezenis SQ 24 and Pseudomonas nitroreducens SQ 63, which are preserved in the China General Microbiological Culture Collection Center with the preservation numbers of CGMCC No.31366, CGMCC No.31369 and CGMCC No.31367 respectively. The three strains of beneficial bacteria have the functions of resisting diseases and promoting growth, can promote the growth of the tuberous roots of Panax notoginseng, increase the saponin accumulation in the roots of Panax notoginseng, prevent the root rot of Panax notoginseng and improve the quality of Panax notoginseng.

This invention relates to the field of microbial technology, and particularly to a soil LiTFSI-degrading bacterium and its application in environmental remediation. The LiTFSI-degrading bacterium is *Pseudomonas nitroreductoides* (Nitroreducing Pseudomonas). Pseudomonas nitroreducens LiT2 or LiT3 were deposited on December 1, 2025, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou, China, with accession numbers GDMCC No: 67389 and GDMCC No: 67390, respectively. This soil LiTFSI-degrading bacterium can use LiTFSI as its sole carbon source and exhibits excellent degradation efficiency for LiTFSI. This invention also provides an environmental remediation agent that can precisely and rapidly intervene in and enhance the degradation process of LiTFSI in soil, thereby significantly reducing the pollution levels of LiTFSI and its intermediate metabolites TFNH2 and TFOH.

The invention discloses pseudomonas nitroreducens GXN01 and application thereof, the pseudomonas nitroreducens GXN01 is preserved in Guangdong Microbial Culture Collection Center on December 20, 2024, the preservation number of the pseudomonas nitroreducens GXN01 is GDMCC NO: 65658, and the preservation address of the pseudomonas nitroreducens GXN01 is the 5th building, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The pseudomonas nitroreducens provided by the invention has a remarkable and rapid degradation effect on quorum sensing signal molecules BDSF, and has huge application potential in the aspect of prevention and treatment of pathogenic bacteria caused by DSF family quorum sensing signal molecules, so that a new possibility is provided for replacing chemical prevention and treatment with biological prevention and treatment; and a new development way is provided for a prevention and control strategy taking a pathogenic bacteria quorum sensing system as a new target.

The invention relates to the technical field of microorganisms, in particular to a soil LiTFSI degrading bacterium and application thereof in the field of environmental remediation. The LiTFSI degrading bacteria are Pseudomonas nitroreducens LiT2 or LiT3, and the LiTFSI degrading bacteria are Pseudomonas nitroreducens LiT2 or LiT3, and the LiTFSI degrading bacteria are Pseudomonas nitroreducens LiT2 or LiT3. The strain is preserved in the Guangdong Microbial Culture Collection Center on December 01, 2025, the preservation address is the 5th floor of the building 59, 100 courtyard, Xianlie Middle Road, Guangzhou City, China, and the preservation numbers are respectively GDMCC No: 67389 and GDMCC No: 67390. The soil LiTFSI degrading bacterium can take LiTFSI as a unique carbon source, and has excellent degradation efficiency on LiTFSI. The invention also provides an environmental remediation microbial agent which can accurately and quickly intervene and strengthen the degradation process of LiTFSI in soil, so that the pollution level of LiTFSI and intermediate metabolites TFNH2 and TFOH thereof is obviously reduced.

The invention discloses an alpha / beta hydrolase as a 3-hydroxypalmitate signal molecule degrading enzyme and application of the alpha / beta hydrolase. Alpha / beta hydrolase is obtained through amplification in pseudomonas nitroreducens, and research finds that the alpha / beta hydrolase is a main quenching enzyme with the capability of degrading 3-hydroxypalmitate signal molecules in the pseudomonas nitroreducens and can effectively degrade quorum sensing signals, namely 3-OH PAME, generated by ralstonia solanacearum, so that the quorum sensing signals generated by ralstonia solanacearum can be effectively degraded, and the quorum sensing signals generated by ralstonia solanacearum can be effectively degraded. Normal expression of ralstonia solanacearum pathogenic factors is interfered, and quorum sensing between ralstonia solanacearum is blocked, so that the pathogenicity of ralstonia solanacearum is inhibited, and plant bacterial wilt is effectively prevented and treated. The invention provides a new target for green prevention and treatment of bacterial wilt, and has a wide application prospect in the field of agricultural biocontrol.

The invention discloses an application of Pseudomonas nitroreducens ZPN-25 in prevention and treatment of cotton diseases, and aims to solve the technical current situation that the application of Pseudomonas nitroreducens in prevention and treatment of cotton diseases is not recorded yet. Separated Pseudomonas nitroreducens ZPN-25 is selected for preparing liquid seeds, 0.9% NaCl normal saline is adopted for culture for resuspension, through root irrigation application, the liquid seeds have prevention and treatment effects on verticillium wilt, fusarium wilt, damping-off and red rot, expression of cotton plant defense related enzymes CAT, PRO and POD and expression quantities of jasmonic acid pathway, salicylic acid pathway and ethylene pathway marker genes can be obviously induced, and the application of the liquid seeds in prevention and treatment of cotton plants is promoted. Therefore, the capability of cotton for resisting pathogenic bacteria is enhanced. The plant height, the leaf area, the stem diameter, the root dry weight, the root length and the overground plant dry weight are all remarkably improved. The pH value and the microbial community structure in a soil sample can be obviously changed, cotton diseases are prevented and controlled by changing the rhizosphere microbial environment, and the method has huge potential development value for green planting of cotton.

The application belongs to the technical field of heavy metal pollution treatment, and relates to a preparation of a weak alkaline soil cadmium passivator and a method for passivating soil lead. The weak alkaline soil cadmium passivator mainly comprises the following components in parts by weight: graphene oxide modified biochar 20-30 parts, montmorillonite 5-10 parts, bentonite 5-10 parts and microbial inoculum 5-10 parts, wherein the microbial inoculum is a composite inoculum composed of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus thuringiensis and Pseudomonas nitroreducens. The weak alkaline soil cadmium passivator has a good repairing effect on cadmium and lead in contaminated soil, can significantly improve the physicochemical properties of cadmium and / or lead contaminated soil, increase the soil organic matter content, reduce the migration in the environment, reduce the cadmium content in crops, and has great market promotion value.

The invention relates to the field of microbial environmental protection technology and agricultural waste treatment, in particular to a deodorizing compound microbial agent as well as a preparation method and application thereof. The microbial agent comprises the following active components: pseudomonas nitroreducens XYT03, and paracoccus pantotrophus XDF08, and the active components of the microbial agent comprise the following components: pseudomonas nitroreducens XYT03, and paracoccus pantotrophus XDF08. The preparation method comprises the following steps: carrying out activation culture on each strain, preparing a seed solution, carrying out fermentation propagation, and mixing fermentation broth of each strain. The microbial inoculum can efficiently degrade ammonia gas, hydrogen sulfide and other malodorous gases generated in the composting process of pig manure through the synergistic effect of all strains. When the microbial agent is applied to pig manure and agricultural waste compost, the emission reduction rate of ammonia gas can reach 30-75%; and the emission reduction rate of hydrogen sulfide reaches 30-65%. The invention effectively solves the problems of single function and insufficient emission reduction efficiency of the existing microbial deodorization fungicide, has the advantages of high deodorization efficiency, strong environmental adaptability, no secondary pollution and the like, and is suitable for environment-friendly treatment and resource utilization of livestock and poultry breeding feces.