39 results about "Pseudomonas nitroreducens" patented technology

The invention belongs to the field of microbial preparations, and in particular relates to a composite microbial preparation for treating domestic sewage and a preparation method thereof. The composite microbial preparation for treating the domestic sewage comprises rhodopseudomonas palustris ACCC10649, saccharomyces cerevisiae ACCC20251, lactobacillus acidophilus ACCC11073, bacillus subtilis ACCC10619, pseudomonas nitroreducens ACCC04184, acinetobacter lwoffii ACCC01091 and candida tropicalis ACCC20004. According to the composite microbial preparation for treating the domestic sewage and the preparation method thereof, which are provided by the invention, organic matters in the sewage are converted into simple inorganic matters through decomposition of microorganisms, so that the sewage is treated effectively.

A microbial process is provided for selective cleavage of only organic C-N bonds while leaving C-C bonds intact which may be used for reducing the nitrogen content of nitrogen-containing organic carbonaceous materials. Microorganisms of Pseudomonas ayucida have been found which have the ability of selective cleavage of organic C-N bonds. A particularly preferred microorganism is Pseudomonas ayucida strain ATCC No PTA-806. Other microorganisms useful in the cleavage of organic C-N bonds are Aneurinibacillus sp, Pseudomonas stutzeri, Yokenella sp. and Pseudomonas nitroreducens.

The invention discloses diffusible signal factor (DSF) quorum sensing signal degradation genes and application thereof. The degradation genes are degradation genes dig1, dig2, dig3 and dig4 separatedfrom pseudomonas nitroreducens bacterial strains HS-18 and having a remarkable degradation effect on quorum sensing signal DSF family signals. The degradation genes are subjected to heterologous expression in nonpathogenic bacteria or biocontrol bacteria, and the degradation ability of the nonpathogenic bacteria or the biocontrol bacteria to DSFs can be remarkably improved; the degradation genes are subjected to heterologous expression in DSF family signal mediated pathogenic bacteria, expression of virulent factors regulated and controlled by the DSF family signals can be remarkably influenced, and the pathogenicity of the pathogenic bacteria to plant hosts is lowered; and thus the DSF quorum sensing signal degradation genes have important practical application value and application prospects in the aspect of biological prevention and treatment of the DSF family signal mediated pathogenic bacteria.

The invention relates to the field of cellulose degradation and discloses pseudomonas nitroreducens as well as a fermentation product and application thereof. The preservation number of the pseudomonas nitroreducens provided by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.13412. The invention further discloses a method for culturing the pseudomonas nitroreducens; the method comprises the following steps: inoculating the pseudomonas nitroreducens into a fermentation culture medium and fermenting. The invention further discloses a method for degrading cellulose and the method comprises the step of enabling a degradation agent to be in contact with a raw material containing the cellulose; the degradation agent is the pseudomonas nitroreducens and / or the fermentation product of the pseudomonas nitroreducens. Furthermore, the invention discloses application of the pseudomonas nitroreducens and / or the fermentation product of the pseudomonas nitroreducens to degradation of the cellulose. The pseudomonas nitroreducens disclosed by the invention can be used for effectively degrading the cellulose, the fermentation product has various enzyme activities and the enzyme activities are relatively high.

The invention discloses a method for preparing theanine by a biological process, which comprises the following steps: preparing cells by using Pseudomonas nitroreducens NTLC4.002 with the collection number of CCTCC NO:M2014254 as the initial strain, and preparing natural theanine by cell transformation. The novel theanine strain applicable to theanine production is separated and screened from the Chinese traditional fermentation product; and the glutaminase generated by the strain can be used for efficiently producing theanine by acting on glutamine and ethylamine under the conditions of pH 9-10 and 35-40 DEG C, wherein the conversion rate can reach more than 80%.

A biological method for removing organic polymers is provided. The method includes the cultivation of a bacterial strain Pseudomonas nitroreducens TX1. The deposit numbers are PTA-6168 at ATCC and BCRC910228 at Bioresources Collection and Research Center in Taiwan (Republic of China). A nitrogen source and inorganic salts are required for bacterial growth. Moreover, the method also provides the information of the supply of air and the water content in soils when this invention is applied to the removal of organic polymers in soils. In addition, the viability of cultivated bacteria is not affected by endogenous bacteria in soils and able to maintain the capacity of degrading organic polymers in the method. The present invention is able to remove the organic polymers in a wide range of amounts effectively and useful in the bioremediation of contamination from organic polymers in both soils and water.

The invention discloses a method for producing L-theanine by using a fermentation method. The method comprises the following steps of: inoculating brevibacteriumflavum into a seed culture medium for expanding culture to obtain first seed liquid; inoculating nitro-reducing pseudomonas into the seed culture medium for expanding culture to obtain second seed liquid; inoculating the first seed liquidand the second seed liquid into a fermentation culture medium containing matcha powder for fermentation to obtain first fermentation liquid, adding ethylamine into the first fermentation liquid, and continuously fermenting and culturing to obtain second fermentation liquid; adding ethylamine into the second fermentation liquid to obtain third fermentation liquid. According to the invention, the glutamine generated by microbial fermentation is directly utilized through the combined fermentation culture of the brevibacteriumflavum and the nitro-reducing pseudomonas, so that the production process is simplified, and the production cost is reduced. According to the invention, the matcha powder is added into the fermentation culture medium, and the ethylamine is added in batches in the fermentation process, so that the fermentation time is shortened, the yield of the L-theanine is improved.

The invention discloses a microbial quorum sensing signal molecule degrading bacterium and its application in disease prevention and control. The nitroreductive pseudomonas is nitroreductive pseudomonas ( Pseudomonas nitroreducens ) strain W‑7, which was deposited in the China Center for Type Culture Collection on November 2, 2017, with the preservation number CCTCC NO: M2017649. The strain has stable activity and can grow with quorum sensing signal molecules AHLs such as OdDHL as the only carbon source, and has a significant and rapid degradation effect on signal molecules. At the same time, the strain has good degradation of other quorum sensing signal molecules such as OHHL and DSF Moreover, the strain also has the characteristics of simple cultivation method, fast growth speed, not easy to mutate, and strong adaptability. It has great potential for popularization and application. The invention can reduce the problem of pesticide abuse, and at the same time provides a new idea for biological control of plant diseases.

The invention provides a kind of nitroreducing Pseudomonas (Pseudomonas nitroreducens) 5-hydroxymethylfurfural oxidase and a preparation method thereof, specifically cloning the DNA sequence of the 5-hydroxymethylfurfural oxidase into a plasmid, And the recombinant plasmid is integrated into the host bacteria to obtain a genetically engineered strain that can express the enzyme heterologously, and the 5-hydroxymethylfurfural oxidase prepared by the heterologous expression of the strain can oxidize 5-hydroxymethylfurfural into 2 ,5‑furandicarboxylic acid, 2,5‑furandicarbaldehyde and 5‑formyl‑2‑furandicarboxylic acid. This patent provides a technical basis for the preparation of furan bulk chemicals by biologically oxidizing 5‑hydroxymethylfurfural.

The present invention belongs to the technical field of molecular biological control. The present invention finds that the nitroreductive Pseudomonas strain HS-18 has good degradation activity to the AHLs with C4-C14 chain length used for detection, and the degradation effect is highly efficient and significant. A new biocontrol agent is provided for the biological control of pathogenic bacteria mediated by AHLs, thereby broadening the types of group quenching bacteria of AHLs signaling molecules.

The invention discloses a pseudomonas nitroreducens and application thereof in degradation of DSF (diffusible signal factor) and / or DSF analogues. The pseudomonas nitroreducens disclosed by the invention is named as HS-18, and is preserved in China Center for Type Culture Collection on May 12, 2017 with a preservation number of CCTCC NO:M2017257. The strain has stable activity, can take DSF as thesole carbon source for growth, can completely degrade DSF within 24h, has a significant and rapid degradation effect on DSF, and has high application value in quorum quenching direction, black rot disease inhibition, and control of the harm of DSF mediation dependent pathogenic bacteria. The pseudomonas nitroreducens provided by the invention can reduce the pesticide abuse problem, and at the same time provides a new idea for biological control of the harm of phytopathogen.

A method for cleavage of the ether-linkage of polyethoxylates is described. A novel pure microbial culture, Pseudomonas nitroreducens TX1 (Depository No.: BCRC910228), is used under aerobic condition to cleave the ether-linkage adjacent to the carboxylated terminus or ethoxyl terminus. This method is useful to cleave sequentially ether-linkage of polyethoxylates in an aqueous buffer solution with an initial concentration of the polyethoxylates between 0.05% and 20%. This method is also effective for the cleavage of the ether-linkages in short-chain polyethoxylates with one to three ethoxyl units.